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pPOTv7-blast-3Ty

Plasmid

#221047

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

3xTy epitopes

Use

Unspecified

Promoter

read through

Available Since

Aug. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pTriEx-mCherry-LOV2 I427V

Plasmid

#81044

Purpose

mammalian expression of LOV2 I427V

Depositor

Klaus Hahn

Article

Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

Insert

LOV I427V

Tags

mCherry

Expression

Bacterial, Insect, and Mamm…

Mutation

I427V

Available Since

Aug. 16, 2016

Availability

Academic Institutions and Nonprofits only

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SchBE4max

Plasmid

#192135

Purpose

Expresses AncBE4max and nSchCas9

Depositor

Yongming Wang

Article

Wang et al Adv Sci (Weinh). 2022 Feb;9(4):e2104789.

Insert

AncBE4max, nSchCas9

Expression

Mammalian

Mutation

SchCas9 (D9A)

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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Halo-Erk2 donor

Plasmid

#113104

Purpose

Donor plasmid for knock-in HaloTag fusing to the N-terminal of mouse Erk2 coding sequence

Depositor

Janet Rossant

Article

Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

Insert

N terminal Erk2 donor region including homology arms and HaloTag

Use

Synthetic Biology

Available Since

Aug. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pOPINVHH_cys_his

Plasmid

#210403

Purpose

For expression of VHH with C-terminal cysteine and his tag

Depositor

Ray Owens

Article

Eyssen et al Bio Protoc. 2024 Mar 20;14(6):e4962. doi

Type

Empty backbone

Tags

cys-his6

Expression

Bacterial

Promoter

lac

Available Since

March 26, 2024

Availability

Academic Institutions and Nonprofits only

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pOPINVHH_sortase_his

Plasmid

#210406

Purpose

For expression of VHH with C-terminal sortase and his tags

Depositor

Ray Owens

Article

Eyssen et al Bio Protoc. 2024 Mar 20;14(6):e4962. doi

Type

Empty backbone

Tags

sortase-his6

Expression

Bacterial

Promoter

lac

Available Since

March 26, 2024

Availability

Academic Institutions and Nonprofits only

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5FAI (EMG1)

Plasmid

#79519

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

EMG1 (EMG1 Human)

Tags

6xHis and TEV cleavage site

Expression

Bacterial

Promoter

T7-lacO

Available Since

June 15, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pGMCS-0X-P38-PanBmGFP

Plasmid

#27127

Depositor

Sabine Ehrt

Article

Gandotra et al PLoS Pathog. 2010 Aug 12;6(8):e1001040.

Insert

PanB

Tags

Linker (SGSGSG), GFPm2+

Expression

Bacterial

Available Since

May 17, 2011

Availability

Academic Institutions and Nonprofits only

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tdminiSOG-C1

Plasmid

#54854

Purpose

Localization: C1 Cloning Vector, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Article

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Type

Empty backbone

Tags

tdminiSOG

Expression

Mammalian

Available Since

June 20, 2014

Availability

Industry, Academic Institutions, and Nonprofits
tdminiSOG-N1

Plasmid

#54855

Purpose

Localization: N1 Cloning Vector, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Article

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Type

Empty backbone

Tags

tdminiSOG

Expression

Mammalian

Available Since

June 20, 2014

Availability

Industry, Academic Institutions, and Nonprofits
IZ1181

Plasmid

#21049

Depositor

Tadahide Izumi

Article

Busso et al Oncogene. 2009 Apr 2. 28(13):1616-25.

Insert

human MDM2 (MDM2 Human)

Tags

no tag

Expression

Mammalian

Mutation

no mutation. This is the wild-type human MDM2.

Available Since

July 9, 2009

Availability

Academic Institutions and Nonprofits only
pTriEx-mCherry-LOV2 V416T

Plasmid

#81040

Purpose

mammalian expression of LOV V416T

Depositor

Klaus Hahn

Article

Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

Insert

LOV2 V416T

Tags

mCherry

Expression

Bacterial, Insect, and Mamm…

Mutation

V416T

Available Since

Aug. 17, 2016

Availability

Academic Institutions and Nonprofits only

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Sa-SchCas9

Plasmid

#192137

Purpose

Expresses Sa-SchCas9, and cloning backbone for sgRNA

Depositor

Yongming Wang

Article

Wang et al Adv Sci (Weinh). 2022 Feb;9(4):e2104789.

Insert

Sa-SchCas9

Use

AAV

Expression

Mammalian

Available Since

March 22, 2023

Availability

Academic Institutions and Nonprofits only

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Phat2-dr-cPla2-C2-mCherry-6H

Plasmid

#187104

Purpose

Protein Expression

Depositor

Philipp Niethammer

Article

Shen et al Proc Natl Acad Sci U S A. 2022 Jan 4;119

Insert

ENSDARG00000024546 (pla2g4aa Zebrafish)

Tags

mCherry

Expression

Bacterial

Available Since

Aug. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pMOD_A3012

Plasmid

#91045

Purpose

Module A, Promoter: PvUbi1, Gene: GFP, Terminator: HSP

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

GFP

Use

Unspecified

Promoter

PvUbi1

Available Since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pMOD_A3010

Plasmid

#91044

Purpose

Module A, Promoter: ZmUbi, Gene: GFP, Terminator: HSP

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

GFP

Use

Unspecified

Promoter

ZmUbi

Available Since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

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pc3XB-ZF5

Plasmid

#13104

Depositor

Keith Joung

Article

Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

Insert

ZF5

Expression

Mammalian

Available Since

Feb. 8, 2007

Availability

Academic Institutions and Nonprofits only

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pFOSGE_COF1G09

Plasmid

#70837

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

TfIIEalpha (TfIIEalpha Fly)

Use

Gateway entry vector

Available Since

Nov. 24, 2015

Availability

Academic Institutions and Nonprofits only
PAS529 (TJF072)

Plasmid

#65603

Purpose

pETDuet-1 Containing FadD Q338R-His

Depositor

Pamela Silver

Article

Ford et al PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717

Insert

FadD

Tags

6x His

Expression

Bacterial

Mutation

Q338R

Promoter

T7 lacO

Available Since

June 3, 2015

Availability

Academic Institutions and Nonprofits only
PAS504 (TJF067)

Plasmid

#65571

Purpose

pETDuet-1 Containing FadD F447S

Depositor

Pamela Silver

Article

Ford et al PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717

Insert

FadD with F447S mutation

Expression

Bacterial

Mutation

F447S

Promoter

T7 lacO

Available Since

June 3, 2015

Availability

Academic Institutions and Nonprofits only