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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 19,393 results for: IRE

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Showing: 10101 - 10150 of 19393 results

  1. pGEX-2T-FRB-W2027F

    Plasmid
    #73341
    Purpose
    Point mutation Trp 2027 → Phe in the FKBP12-rapamycin binding (FRB) domain of mTOR
    Depositor
    Jie Chen
    Article
    Vilella-Bach et al J Biol Chem. 1999 Feb 12. 274(7):4266-72
    Insert
    FKBP12-rapamycin binding (FRB) domain of MTOR (MTOR Human)
    Tags
    GST-tagged
    Expression
    Bacterial
    Mutation
    FRB domain only aa2015-2114; W2027F
    Available Since
    April 19, 2016
    Availability
    Academic Institutions and Nonprofits only

  15. pLVX-TetOneZeo-ORF18(E36A_L37A)-3xFLAG

    Plasmid
    #129758
    Purpose
    Dox inducible expression of KSHV ORF18-3xFLAG (E36A_L37A) in mammalian cells
    Depositor
    Britt Glaunsinger
    Article
    Castaneda et al J Virol. 2018 Oct 10. pii: JVI.01488-18.
    Insert
    ORF18 (ORF18 Kaposi's sarcoma-associated herpesvirus (HHV-8))
    Use
    Lentiviral
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    changed Glutamic acid 36 to Alanine and Leucine 3…
    Promoter
    TRE3Gs and Tet inducible promoter
    Available Since
    Sept. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    129758-plasmid-map-sequence-id-257095

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  24. pGEX-2T FRB Y2105A

    Plasmid
    #68726
    Purpose
    Point mutation Tyr 2105 → Ala in the FKBP12-rapamycin binding (FRB) domain of mTOR
    Depositor
    Jie Chen
    Article
    Vilella-Bach et al J Biol Chem. 1999 Feb 12. 274(7):4266-72
    Insert
    FKBP12-rapamycin binding (FRB) domain of MTOR (MTOR Human)
    Tags
    GST-tagged
    Expression
    Bacterial
    Mutation
    FRB domain only aa2015-2114; Y2105A
    Available Since
    April 19, 2016
    Availability
    Academic Institutions and Nonprofits only

  27. pNB785

    Plasmid
    #60164
    Purpose
    Firefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion with PEST degron driven by SIC1 promoter.
    Depositor
    Nicolas Buchler
    Article
    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14
    Insert
    FLuc-yEVenus-PEST
    Tags
    PEST degron
    Expression
    Yeast
    Mutation
    FLuc has A4V & S504G (no functional effect)
    Promoter
    SIC1pr
    Available Since
    March 25, 2015
    Availability
    Academic Institutions and Nonprofits only
    60164-plasmid-map-sequence-id-96373

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  33. pNB787

    Plasmid
    #60152
    Purpose
    Firefly luciferase (Promega) and yeast codon-optimized yellow fluorescent protein (yEVenus) fusion with PEST degron driven by LEU1 promoter.
    Depositor
    Nicolas Buchler
    Article
    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14
    Insert
    FLuc-yEVenus-PEST
    Tags
    PEST degron
    Expression
    Yeast
    Mutation
    FLuc has A4V & S504G (no functional effect)
    Promoter
    LEU1pr
    Available Since
    March 25, 2015
    Availability
    Academic Institutions and Nonprofits only
    60152-plasmid-map-sequence-id-96317

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  39. pNB789

    Plasmid
    #60166
    Purpose
    Firefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion with PEST degron driven by RNR1 promoter.
    Depositor
    Nicolas Buchler
    Article
    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14
    Insert
    FLuc-yEVenus-PEST
    Tags
    PEST degron
    Expression
    Yeast
    Mutation
    FLuc has A4V & S504G (no functional effect)
    Promoter
    RNR1pr
    Available Since
    March 25, 2015
    Availability
    Academic Institutions and Nonprofits only
    60166-plasmid-map-sequence-id-96375

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Showing: 10101 - 10150 of 19393 results