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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 12,282 results for: LEA

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Showing: 4061 - 4080 of 12282 results

  1. DHFR-GFP-th-NL1

    Plasmid
    #173007
    Purpose
    For light regulated release of neuroligin 1 from the endoplasmic reticulum. Driven by synapsin promoter. Thrombin site is included between GFP and NL1.
    Depositor
    Matthew Kennedy
    Publication
    Bourke et al J Cell Biol. 2021 Sep 6;220(9). pii: 212
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    DHFR-GFP-NL1
    Tags
    DHFR and EGFP
    Expression
    Mammalian
    Promoter
    human synapsin
    Available since
    Aug. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    173007-plasmid-map-sequence-id-339111

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  4. pmSod1-G93A-N’His

    Plasmid
    #117705
    Purpose
    mutation on stability/solubility of SOD1
    Depositor
    Elizabeth Fisher
    Insert
    Sod1 (Sod1 Mouse)
    Tags
    His tag with Thrombin cleavage site
    Expression
    Bacterial
    Mutation
    G93A
    Promoter
    T7
    Available since
    Nov. 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    117705-plasmid-map-sequence-id-227526

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  6. pTE3330

    Plasmid
    #107534
    Purpose
    Expresses Lb crRNA and human codon optimized LbCpf1(RVR mutant) in mammalian cells.
    Depositor
    Ervin Welker
    Publication
    Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510
    Inserts
    Lb crRNA
    hLbCpf1(RVR mutant)
    Tags
    3xHA and NLS
    Expression
    Mammalian
    Mutation
    G532R, K538V, Y542R
    Promoter
    CMV and human U6
    Available since
    March 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    107534-plasmid-map-sequence-id-207314

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  9. pCL1271

    Plasmid
    #217963
    Purpose
    Mammalian expression of Importin 5 (Imp5, IPO5) with an N-terminal eGFP fusion. Splice isoform 1.
    Depositor
    Andrew Bowman
    Publication
    Apta-Smith et al EMBO J. 2018 Oct 1;37(19):e98714. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Imp5 (IPO5 Human)
    Tags
    eGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    April 18, 2024
    Availability
    Academic Institutions and Nonprofits only
    217963-plasmid-map-sequence-id-430217

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  12. pCAG-AsCas12a-Plus-mCherry

    Plasmid
    #191231
    Purpose
    To express the AsCas12a nuclease variant "AsCas12a-Plus" that enhanced the editing activity and specificity
    Depositor
    Zhili Rong
    Publication
    Huang et al BMC Biol. 2022 Apr 25;20(1):91. doi: 10.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    AsCas12a-Plus-P2A-mCherry
    Use
    CRISPR
    Mutation
    E174R, R951K, R955A
    Available since
    May 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    191231-plasmid-map-sequence-id-392988

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  18. pASPIre2

    Plasmid
    #154843
    Purpose
    Derivative of pASPIre1 carrying a bxb1-sfGFP translational fusion in place of bxb1
    Depositor
    Markus Jeschek
    Publication
    Hollerer et al Nat Commun. 2020 Jul 15;11(1):3551. doi:
    Insert
    Bxb1-sfGFP fusion controlled by rhamnose-inducible promoter; attB/attP-flanked discriminator containing mCherry; rhaRS operon
    Expression
    Bacterial
    Available since
    Aug. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    154843-plasmid-map-sequence-id-307634

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Showing: 4061 - 4080 of 12282 results