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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 31,681 results for: PLE

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Showing: 4081 - 4100 of 31681 results

  3. pSBbi-TIR1-tTA/Pur

    Plasmid
    #171683
    Purpose
    constitutive expression of TIR1-myc and tTA in sleeping beauty transposon vector with selection marker (puromycin)
    Depositor
    Randy Poon
    Article
    Yeung et al Mol Biol Cell. 2021 May 12:mbcE21020051.
    Inserts
    tetracycline-controlled transactivator
    Puromycin
    EF-1a promoter
    Expression
    Mammalian
    Promoter
    EF-1a promoter and RPBSA promoter
    Available Since
    Oct. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    171683-plasmid-map-sequence-id-338224

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  5. pDAC565

    Plasmid
    #195242
    Purpose
    Mammalian expression of Csm-GFP complex (RNase mut) from separate promoters (Used for RNA imaging)
    Depositor
    Jennifer Doudna
    Article
    Colognori et al Nat Biotechnol. 2023 Jan 23. doi: 10.103
    Insert
    FLAG-NLS-Csm1; FLAG-NLS-Csm2, FLAG-NLS-Csm3(RNase mut)-GFP; FLAG-NLS-Csm4; FLAG-NLS-Csm5; FLAG-NLS-Cas6; crRNA
    Expression
    Mammalian
    Available Since
    Jan. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    195242-plasmid-map-sequence-id-382950

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  10. pTP1174

    Plasmid
    #104162
    Purpose
    Bacterial expression plasmid of anti-mouse kappa chain nanobody TP1170 (1x Cysteine)
    Depositor
    Dirk Görlich
    Article
    Pleiner et al J Cell Biol. 2018 Mar 5;217(3):1143-1154
    Insert
    Anti-mouse kappa chain specific nanobody TP1170 (1xCysteine)
    Tags
    14xHistidine tag and NEDD8 from Brachypodium dist…
    Expression
    Bacterial
    Available Since
    Dec. 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    104162-plasmid-map-sequence-id-200079

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  14. pLV hU6-gRNA(anti-sense) hUbC-VP64-dCas9-VP64-T2A-GFP

    Plasmid
    #66707
    Purpose
    expresses a single gRNA and VP64-dCas9-VP64
    Depositor
    Charles Gersbach
    Inserts
    hU6-gRNA expression cassette
    S.Pyogenes VP64-dCas9-VP64
    Use
    Lentiviral and Synthetic Biology
    Tags
    VP64
    Mutation
    D10A and H840A
    Promoter
    hU6 and hUbC
    Available Since
    April 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    66707-plasmid-map-sequence-id-244291

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  17. RNA-Protein Landing Pad

    Plasmid
    #226162
    Purpose
    Used to generate a landing pad in Flp-In cells containing promoters for protein and MS2-circRNA barcode expression
    Depositor
    Dustin Maly
    Article
    Simon et al Nat Methods. 2024 Nov;21(11):2094-2106.
    Inserts
    mTagBFP2-P2A-iCasp9
    Reverse tetracycline transactivator 3
    Hygromycin-IRES-EGFP
    Use
    Synthetic Biology
    Tags
    HA
    Expression
    Mammalian
    Available Since
    Oct. 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    226162-plasmid-map-sequence-id-450826

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Showing: 4081 - 4100 of 31681 results