We narrowed to 19,248 results for: Tat
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Plasmid#83503PurposeGeneration of transgenic mice overexpressing the HGF under the control of the WAP gene promoterDepositorInsertHGF (Hgf Mouse)
UseMouse TargetingMutationalso contains beta-globin sequences from pUC198Promotermouse WAP (whey acidic protein)Available SinceFeb. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
Flag_HsIRE1a_deltaP29_D408_K599A_pBabePuro
Plasmid#58492Purposeretroviral pBabe puro vector encoding N-terminally FLAG tagged mutant human IRE1a deleted from amino acids P28 to D408 and bearing mutation K599A (kinase domain mutant)DepositorInsertIRE1a (ERN1 Human)
UseRetroviralTagsFLAG and preprotrypsin signal sequenceExpressionMammalianMutationdeleted amino acids P29 to D408 and bearing mutat…Available SinceSept. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX6-P3nmt1-3HA
Plasmid#39283DepositorInsertnmt1 promoter (nmt1 Fission Yeast)
UseYeast genomic targetingTags3xHA, A. gossypii translation elongation factor 1…Mutation-1163 to +6 of nmt1 locusAvailable SinceAug. 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
p3xFLAG-CMV-Tlr4(Lps)
Plasmid#27149DepositorInserttoll-like receptor 4(Lps) (Tlr4 Mouse)
Tags3x FLAGExpressionMammalianMutationchanged Proline 712 to Histidine. Native signal p…Available SinceFeb. 10, 2011AvailabilityAcademic Institutions and Nonprofits only -
CBP-Bromo-HAT-CH3-sp65
Plasmid#21026DepositorInsertCBP (Crebbp Mouse)
UseIn vitro tntTagsFlagMutationBromo+HAT+CH3 domains aa 1098-1877; E1239G mutati…Available SinceAug. 14, 2009AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO HIS HSPA1L
Plasmid#19538DepositorAvailable SinceOct. 6, 2008AvailabilityAcademic Institutions and Nonprofits only -
pSH-CAG-AtAFB2.F74A-mCherry-IRES-puro
Plasmid#216244PurposeExpress AtAFB2.F74A mutant with mCherry tag for AID2DepositorInsertAtAFB2.F74A-mCherry
UseCRISPR, TALEN, and Unspecified ; Human safe harbo…TagsmCherryExpressionMammalianMutationF74A mutationPromoterCAGAvailable SinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMega-MaFRSA
Plasmid#200226PurposeThe plasmid encodes an orthogonal aminoacyl-tRNA synthetase/tRNA-CUA pair for amber codon suppression with meta-trifluoromethylphenylalanine..DepositorInsertsPyrrolysyl-tRNA synthetase
pyrrolysyl-tRNA
TagsnoneExpressionBacterialMutationmutated Asparagine 166 to Alanine and Valine 168 …PromoterproK-lacO and tacIAvailable SinceJune 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPGK-T7/2-CD44v9 (fl)
Plasmid#137818Purposeexpression of CD44 proteins in mammalian cellsDepositorInsertCD44v9 (fl) (CD44 Human)
TagsGFPExpressionMammalianMutationfull length and mutation H48LPromoterPGKAvailable SinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLL1 sgRNA(MS2)-MS2-AIDmono
Plasmid#112127Purposeempty sgRNA cloning vector with MS2-AIDmonoDepositorInsertAID mono mutant (AICDA Human)
UseCRISPRExpressionMammalianMutationN- mutated and C- terminal truncated, H130A/R131E…PromoterhU6,CBhAvailable SinceSept. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-DIO {hCAR}off-{ChETA-mRuby2}on-W3SL
Plasmid#111391PurposeAAV vector with hSynapsin promoter, Cre-OFF hCAR (for efficient CAV-2 infection), Cre-ON ChETA-mRuby2 (for optogenetic activation), and W3SL regulatory cassette (for maximize cloning capacity)DepositorInsertsUseAAVTagsMyc and mRuby2ExpressionMammalianPromoterhSynapsinAvailable SinceJune 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTO_CHMP2B_LAP
Plasmid#101848PurposeTet-inducible expression of human CHMP2B with a C-terminal GFP and long linker (LAP tag), compatible with Flp-In recombination for stable integrationDepositorInsertCHMP2B (CHMP2B Human)
UseFlp-frt recombination, tet-inducible expressionTagsLocalization and Affinity Purification (LAP) tagExpressionBacterial and MammalianMutationsilent mutation in Thr 104; A to TPromoterCMVAvailable SinceDec. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
Delta DED FLIP-L D/A
Plasmid#13791DepositorInsertDELTA DED FLIP LONG (CFLAR Human)
Tags8 x His tagExpressionBacterialMutationDelta DED removes first 186 residues Asp to Ala m…Available SinceJan. 25, 2007AvailabilityAcademic Institutions and Nonprofits only -
pGH220_wtGFP
Plasmid#85410Purposelentiviral expression vector for wtGFPDepositorInsertwtGFP
UseLentiviralExpressionMammalianAvailable SinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
hNTo1-qgRNA-pYJA5
Plasmid#217781PurposeNon-targeting control 1 qgRNA-pYJA5 plasmid from the T.spiezzo library; it can be used as a ready-to-go control and provides the three constant regions for the three-fragment PCRs for qgRNA cloningDepositorInsertQuadruple sgRNA-expression cassette for nontargeting control for gene ablation
UseCRISPR, Lentiviral, and Synthetic BiologyExpressionMammalianPromoterhuman U6, mouse U6, human H1, human 7SKAvailable SinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pPMQAK1-Ptrc-[E*](104)-Cas9-B0015-lacZ-PnrsB-mazF-LVA
Plasmid#190790Purpose[E*](104) construct. Theophylline inducible Cas9 (S. pyogenes) expression. Two BsaI-sites allow for simultaneous insertion of sgRNA and donor DNA. Includes a nickel-inducible curing system.DepositorInsertsCas9
mazF-LVA
UseCRISPR and Synthetic BiologyTagsLVAExpressionBacterialMutationsilent mutations of BpiI-sites in original Cas9 g…PromoterPnrsB and Ptrc-[E*](104)Available SinceFeb. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL3-sgRab18.N-Cas9-T2A-mCherry-P2A-Puro
Plasmid#129418PurposeEncoding Cas9 and sgRAB18.N for CRISPR/Cas9 mediated HDR tagging of endogenous human RAB18 N-terminusDepositorInsertSpCas9 and sgRAB18.N (RAB18 Human)
UseCRISPRTagsT2A-mCherry-P2A-PuroExpressionMammalianPromoterhU6Available SinceAug. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
Desmoplakin I tension sensor (F40-based)
Plasmid#119186PurposeThe F40-based human desmoplakin I tension sensor detects forces in the range of 1-6 pN by changes in FRET between mTFP1 and mEYFP.DepositorInserthuman Desmoplakin I-[mTFP1-F40-mEYFP] (internal-1945) (DSP Human)
UseTransposonTagsmTFP1-F40-mEYFPExpressionMammalianMutationinserted F40-based tension sensor module after aa…PromoterTCEAvailable SinceDec. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pShuttle-EGFP-CLASP2 340-1362 9xS/A
Plasmid#24373DepositorInsertCLASP2 (340-1362) 9xS/A (CLASP2 Human)
TagsEGFPExpressionMammalianMutationNonphosphorylatable CLASP2 deletion mutant. M…Available SinceJune 29, 2010AvailabilityAcademic Institutions and Nonprofits only -
pBabe-TetCMV-puro-mCerulean-PA-Rac1
Plasmid#22031DepositorInsertmCerulean-PA-Rac1 (RAC1 Human, Avena sativa (oat))
UseRetroviralExpressionMammalianMutationRac1 starts at I4 and contains mutations Q61L, E9…Available SinceSept. 29, 2009AvailabilityAcademic Institutions and Nonprofits only