We narrowed to 7,158 results for: ef1a

1. pLenti-EF1a-Backbone(NG)

   Plasmid

   #27963

   Depositor

   Feng Zhang

   Article

   Zhang et al Nat Biotechnol. 2011 Feb;29(2):149-53. d

   Insert

   TAL-Backbone(NG)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 18, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEF1a-Usp9x_CD-mCherry

   Plasmid

   #170380

   Purpose

   Vector for mammalian expression of the Usp9x catalytic domain tagged with mCherry, in pEF1a-IRES-Neo backbone.

   Depositor

   Miguel Ramalho-Santos

   Article

   Macrae et al Nat Commun. 2021 Mar 25;12(1):1865. doi:

   Insert

   Ubiquitin specific protease 9x catalytic domain (Usp9x Mouse)
   

   Use

   Tags

   mCherry-3xFlag

   Expression

   Mammalian

   Mutation

   Catalytic domain

   Promoter

   Available since

   June 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pKLV2-EF1a-Cas9GFP-W

   Plasmid

   #200100

   Purpose

   Lentiviral vector expressing Cas9 fused with GFP at the C-terminus

   Depositor

   Kosuke Yusa

   Article

   Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

   Insert

   GFP
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHL-EF1a-SphcCas9-iP-A

   Plasmid

   #60599

   Purpose

   Expresses human codon-optimized Cas9 (derived from Streptococcus pyogenes) and pruomycin resistance gene.

   Depositor

   Akitsu Hotta

   Article

   Li et al Stem Cell Reports. 2014 Nov 25. pii: S22

   Insert

   CRISPR Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Codon usage optimized for human usage

   Promoter

   Available since

   Dec. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEF1alpha-mClover3-bGHpA

   Plasmid

   #172286

   Purpose

   Constitutive expression of mClover3

   Depositor

   Patrik Verstreken

   Insert

   mClover3
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   EF1alpha

   Available since

   Aug. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLV-EF1a-V5-LIC

   Plasmid

   #120247

   Purpose

   For LIC cloning with N-terminal V5 tag

   Depositor

   Larry Gerace

   Article

   Cheng et al Nucleus. 2019 Dec;10(1):126-143. doi: 10

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mutation

   Promoter

   Available since

   Jan. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. PlayBack-Ef1a-EcoR1

   Plasmid

   #203311

   Purpose

   For expression of eukaryotic genes of interest with Ef1a promoter with EcoRI as a PlayBack Restriction Enzyme

   Depositor

   Aleksandar Necakov

   Article

   Foran et al Biotechniques. 2023 Oct;75(4):168-178. d

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHAGE-EF1alphaL-hAAT-W

   Plasmid

   #24527

   Purpose

   Lentiviral overexpression

   Depositor

   Darrell Kotton

   Article

   Wilson et al J Clin Invest. 2010 Jan . 120(1):379-89.

   Insert

   alpha-1 antitrypsin (SERPINA1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 30, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEF1alpha-rtTA-Antares

   Plasmid

   #197587

   Purpose

   Expresses TET-On reverse transactivator labeled with Antares

   Depositor

   Mikhail Shapiro

   Article

   Hurt et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   rtTA-P2A-Antares
   

   Use

   Tags

   Antares

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1alpha

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pENTR-EF1adCas9VP64_T2A_MS2p65HSF1-IRESbsdpA

    Plasmid

    #112922

    Purpose

    Gateway entry vector carrying human EF1a promoter-driven dCas9VP64-T2A-MS2p65HSF1 with Blasticidine resistant marker

    Depositor

    Kosuke Yusa

    Article

    Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

    Insert

    EF1a promoter, dCas9VP94, MS2p65HSF1, IRESneo
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEF1a_FL MUT TET1

    Plasmid

    #124081

    Purpose

    Expression of catalytic inactive form of full-length TET1

    Depositor

    Silvia Monticelli

    Article

    Vincenzetti et al Eur J Immunol. 2019 Jan 30. doi: 10.1002

    Insert

    TET1 - full length, mutated (TET1 Human)
    

    Use

    Tags

    Flag and HA

    Expression

    Mammalian

    Mutation

    H1672Y, D1674A

    Promoter

    EF1 alpha

    Available since

    April 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHeEf1a-mEGFP-NLS

    Plasmid

    #205012

    Purpose

    The vector contains 1kbp of the upstream and downstream regions of the ef1a gene from Hypsibius exemplaris, with the mEGFP gene with NLS sequence positioned in between.

    Depositor

    Kazuharu Arakawa

    Article

    Tanaka et al Proc Natl Acad Sci U S A. 2023 Jan 31;12

    Insert

    mEGFP-NLS flanked by 1kbp upstream and downstream of the ef1a gene from Hypsibius exemplaris
    

    Use

    Tardigrade expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHR_EF1a-->sGFP_PGK-->puroR

    Plasmid

    #162156

    Purpose

    Lentiviral vector for constitutive expression of GFP in mammalian cells

    Depositor

    Wendell Lim

    Article

    Toda et al Science. 2020 Oct 16;370(6514):327-331.

    Insert

    EGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-EF1a-sCre

    Plasmid

    #55635

    Purpose

    Expresses sCre in Mammalian Cells

    Depositor

    Karl Deisseroth

    Article

    Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

    Insert

    Cre
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    July 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. LV_EF1a_MAPK14-P2A-Hygro_Barcode

    Plasmid

    #170239

    Purpose

    Barcoded lentiviral vector to express MAPK14 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

    Depositor

    Prashant Mali

    Article

    Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

    Insert

    MAPK14 (MAPK14 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLVH-EF1A-BRD3R

    Plasmid

    #130696

    Purpose

    Lentiviral plasmids encoding human BRD3R

    Depositor

    Kejin Hu

    Article

    Shao et al Nat Commun. 2016 Mar 7;7:10869. doi: 10.

    Insert

    Human BRD3R (BRD3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pT3-EF1A-CCNE1

    Plasmid

    #162553

    Purpose

    Overexpression of CCNE1

    Depositor

    Amaia Lujambio

    Article

    Molina-Sanchez et al Gastroenterology. 2020 Aug 16. pii: S001

    Insert

    Overexpression of CCNE1 (CCNE1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    None

    Promoter

    EF1A

    Available since

    Feb. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pKLV-EF1a-BFP-W

    Plasmid

    #208757

    Purpose

    Lentiviral vector expressing BFP

    Depositor

    Kosuke Yusa

    Article

    Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pHAGE-EF1aL-nlsGFP-W

    Plasmid

    #126688

    Purpose

    Constitutive, ubiquitous, nuclear-localized GFP expression.

    Depositor

    Darrell Kotton

    Article

    Tang et al Nat. Phys. 18, 1371–1378 (2022).

    Insert

    nlsGFP
    

    Use

    Lentiviral

    Tags

    6xHIS and NLS

    Expression

    Mutation

    Promoter

    EF1aL

    Available since

    June 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEF1a_CD MUT TET1

    Plasmid

    #124083

    Purpose

    Expression of mutated catalytic domain only form of TET1

    Depositor

    Silvia Monticelli

    Article

    Vincenzetti et al Eur J Immunol. 2019 Jan 30. doi: 10.1002

    Insert

    TET1 - catalytic domain mutated (TET1 Human)
    

    Use

    Tags

    Flag and HA

    Expression

    Mammalian

    Mutation

    H1672Y, D1674A

    Promoter

    EF1 alpha

    Available since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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