We narrowed to 22,015 results for: cas9 genes

1. pAAV-FLEX-SaCas9-U6-sgGria2

   Plasmid

   #124854

   Purpose

   Mutagenesis of Gria2

   Depositor

   Larry Zweifel

   Article

   Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

   Insert

   Gria2 (Gria2 Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. xCas9(3.6)-ABE(7.10)

   Plasmid

   #108387

   Purpose

   Mammalian expression vector for xCas9(3.6)-ABE

   Depositor

   David Liu

   Article

   Hu et al Nature. 2018 Feb 28. pii: nature26155. d

   Insert

   xCas9(3.6)-ABE(7.10)
   

   Use

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   E108G, S217A, A262T, S409I, E480K, E543D, M694I, …

   Promoter

   Available since

   March 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. AAV_NLS-SaCas9-NLS-VPR

   Plasmid

   #68496

   Purpose

   AAV vector containing SaCas9 fused to VPR

   Depositor

   George Church

   Article

   Kiani et al Nat Methods. 2015 Sep 7. doi: 10.1038/nm

   Insert

   SaCas9-VPR
   

   Use

   AAV and CRISPR

   Tags

   VPR

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. hRad51(K133R)–Cas9(D10A)

   Plasmid

   #125568

   Purpose

   Expresses the Rad51(K133R)–Cas9(D10A) fusion construct in mammalian cells under CMV promoter

   Depositor

   David Liu

   Article

   Rees et al Nat Commun. 2019 May 17;10(1):2212. doi:

   Insert

   Rad51(K133R)–Cas9(D10A) (RAD51 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPB-VPR-dSpCas9[StaPL(TI)]

   Plasmid

   #111508

   Purpose

   Expresses a VPR transcriptional activator fused to the nuclease-deactivated S. pyogenes Cas9, which is governed by an inserted StaPL(TI) module, in mammalian cells. [TI = telaprevir inhibited].

   Depositor

   Michael Lin

   Article

   Jacobs et al Nat Methods. 2018 Jul;15(7):523-526. doi

   Insert

   VPR-dSpCas9[StaPL(TI)]
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   The HCV NS3 protease carries F43L, Q80K, and S122…

   Promoter

   PGK

   Available since

   July 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-FLEXfrt-SaCas9-U6-sgSlc17a8

   Plasmid

   #124861

   Purpose

   Mutagenesis of Slc17a8

   Depositor

   Larry Zweifel

   Article

   Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

   Insert

   Slc17a8 (Slc17a8 Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. p2T-CAG-KKHSaCas9-BlastR

   Plasmid

   #107189

   Purpose

   Confers constitutive expression of KKH SaCas9.

   Depositor

   Richard Sherwood

   Article

   Shen et al Nature. 2018 Nov;563(7733):646-651. doi:

   Insert

   KKH SaCas9 (NEWENTRY )
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   E782K/N968K/R1015H

   Promoter

   Chicken ß-Actin

   Available since

   Dec. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-SMVP-Cas9N-U6-gRNA P2

   Plasmid

   #80944

   Purpose

   Expresses Cas9N in mammalian cells; expresses gRNA P2 for Pd-l1 binding

   Depositor

   George Church

   Article

   Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

   Insert

   Cas9N
   

   Use

   AAV, CRISPR, and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pX330-Flag-wtSpCas9-H840A

   Plasmid

   #80453

   Purpose

   SpCas9 mammalian expression vector

   Depositor

   Ervin Welker

   Article

   Simon et al Elife. 2022 Feb 23;11. pii: 69504. doi:

   Insert

   SpCas9_H840A
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available since

   Aug. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET-FLAG-SpCas9-HF1

    Plasmid

    #126770

    Purpose

    Expression of increased fidelity SpCas9-HF1 in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

    Insert

    SpCas9-HF1
    

    Use

    CRISPR

    Tags

    3xFLAG, 6xHis, MBP, and NLS

    Expression

    Bacterial

    Mutation

    N497A, R661A, Q695A, Q926A

    Promoter

    T7

    Available since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. p-dCas9-LSD1-K661A-Hygro

    Plasmid

    #104414

    Purpose

    transient expression of dCas9-dLSD1 fusion protein

    Depositor

    Stephan Beck

    Insert

    dLSD1 (KDM1A Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    IRATp970A0364D (Source Bioscience) has a P405H ch…

    Promoter

    CMV promoter

    Available since

    April 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. 3xFLAG-dCas9/pTEF1p-CYC1t

    Plasmid

    #62190

    Purpose

    Expresses 3xFLAG-dCas9 in budding yeast for enChIP analysis to purify specific genomic regions of interest.

    Depositor

    Hodaka Fujii

    Article

    Fujii et al Biol Methods Protoc. 2022 Oct 17;7(1):bp

    Insert

    3xFLAG-dCas9
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Yeast

    Mutation

    human codon-optimized, D10A, H840A

    Promoter

    TEF1 promoter

    Available since

    Feb. 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pU6-(BsaI)_CBh-UN-Cas9

    Plasmid

    #135011

    Purpose

    Expression vector for sgRNAs cloned into the BsaI sites and for expression of Cas9 with 126aa MRN-recruiting domain from HSV-1 UL12 fused to N-terminus of Cas9

    Depositor

    Yosef Shaul

    Article

    Reuven et al Biomolecules. 2019 Oct 8;9(10). pii: bio

    Insert

    humanized S. pyogenes Cas9
    

    Use

    Tags

    126aa domain from HSV-1 UL12 fused to the N-termi…

    Expression

    Mammalian

    Mutation

    126aa domain from HSV-1 UL12 fused to the N-termi…

    Promoter

    CBh

    Available since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-FLEXfrt-SaCas9-U6-sgGria1

    Plasmid

    #124867

    Purpose

    Mutagenesis of Gria1

    Depositor

    Larry Zweifel

    Article

    Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

    Insert

    Gria1 (Gria1 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. PB-tight-DDdCas9GFP-IRES-Neo

    Plasmid

    #102907

    Purpose

    PiggyBac construct with doxycyline inducible TRE-tight promoter expression of dCas9-GFP followed by IRES-Neomycin selection cassette.

    Depositor

    Timo Otonkoski

    Article

    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

    Insert

    DDdCas9GFP-IRES-Neo
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    TRE-tight

    Available since

    July 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. MiniCoopR U6:gRNA cdkn2a, mitfa:Cas9

    Plasmid

    #118842

    Purpose

    Targets cdkn2a and expresses zebrafish mitfa specifically in zebrafish melanocytes

    Depositor

    Leonard Zon

    Article

    Ablain et al Science. 2018 Nov 1. pii: science.aau650

    Insert

    cdkn2a gRNA (LOC100329528 Zebrafish)
    

    Use

    CRISPR; Tol2

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. PB-CMV-NLS-dCas9-NLS

    Plasmid

    #99908

    Purpose

    For the expression of NLS-dCas9-NLS

    Depositor

    Yujie Sun

    Article

    Shao et al Nucleic Acids Res. 2016 May 19;44(9):e86

    Insert

    NLS-dCas9-NLS
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLenti-xCas9RA-P2A-Puro

    Plasmid

    #110871

    Purpose

    Lentiviral vector for constitutive expression of xCas9(3.7)RA in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    xCas9(3.7)RA
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    A262T, R324L, S409I, E480K, E543D, M694I, E1219V

    Promoter

    EF1s

    Available since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-FLEX-SaCas9-U6-sgSlc17a7

    Plasmid

    #124846

    Purpose

    Mutagenesis of Slc17a7

    Depositor

    Larry Zweifel

    Article

    Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

    Insert

    Slc17a7 (Slc17a7 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. PX429 SpCas9 N863A nickase

    Plasmid

    #68706

    Purpose

    SpCas9 N863A nickase

    Depositor

    Feng Zhang

    Article

    Nishimasu et al Cell. 2014 Feb 27;156(5):935-49. doi: 10

    Insert

    SpCas9 N863A nicking mutant
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Cbh

    Available since

    Sept. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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