168,234 results

1. pMTS-HA-GFP11

   Plasmid

   #177725

   Purpose

   Eleventh β-strand of GFP (GFP11) fused to a mitochondria signal sequence for mitochondrial targeting, HA tagged

   Depositor

   Erik Böttger

   Article

   Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:

   Insert

   MTS-HA-GFP11
   

   Tags

   GFP11 and HA

   Expression

   Bacterial and Mammalian

   Available Since

   Jan. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1-HA-RAD52-WT

   Plasmid

   #235676

   Purpose

   Expresses HA-tagged wild type human RAD52 in mammalian cells.

   Depositor

   Simon Powell

   Article

   Jalan et al Nat Commun. 2024 Sep 5;15(1):7776. doi:

   Insert

   RAD52 (RAD52 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   wild type

   Available Since

   April 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA5/FRT/TO(Neo)-Venus-Flag-WT wobbled cdc20 (1991)

   Plasmid

   #61848

   Purpose

   for making stable RPE cells expressing WT cdc20

   Depositor

   Jonathon Pines

   Insert

   cdc20 (CDC20 Human)
   

   Tags

   Flag and Venus

   Expression

   Mammalian

   Mutation

   wobbled

   Promoter

   CMV

   Available Since

   Feb. 4, 2015

   Availability

   Academic Institutions and Nonprofits only

4. GreenGate 2.0 Toolkit

   Plasmid Kit

   #1000000241

   Purpose

   The GreenGate 2.0 (GG2.0) toolkit enables the assembly and stacking of complex transcriptional units with the GreenGate Cloning System. It is backwards compatible with most existing GreenGate modules.

   Depositor

   Alexis Maizel

   Available Since

   April 11, 2024

   Availability

   Academic Institutions and Nonprofits only

5. pcDNA3.3_eGFP

   Plasmid

   #26822

   Depositor

   Derrick Rossi

   Article

   Warren et al Cell Stem Cell. 2010 Nov 5;7(5):618-30.

   Insert

   5'UTR-eGFP-3'UTR
   

   Expression

   Mammalian

   Available Since

   Nov. 17, 2010

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. sfTq2ox-PBP5

   Plasmid

   #117960

   Purpose

   Periplasmic expression of superfolder mTurquoise2ox (pNM077 pTHV037-DsbAss-LEGPAGL-sfTq2ox-EFGS-∆1-17-PBP5)

   Depositor

   Tanneke den Blaauwen

   Article

   Meiresonne et al Mol Microbiol. 2019 Apr;111(4):1025-1038

   Insert

   sfTq2ox-PBP5 (dacA )
   

   Expression

   Bacterial

   Available Since

   Jan. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMTU-DO-HPH-C4

   Plasmid

   #160343

   Purpose

   High-copy expression vector for K. marxianus, hygromycin selection

   Depositor

   John Morrissey

   Article

   Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

   Type

   Empty backbone

   Use

   Kluyveromyces marxianus

   Expression

   Yeast

   Promoter

   n/a

   Available Since

   Nov. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRG2

   Plasmid

   #104174

   Purpose

   sgRNA expression

   Depositor

   Jin-Soo Kim

   Article

   Kim et al Genome Biol. 2017 Nov 15;18(1):218. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Dec. 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCW57.1_AOX-FLAG

   Plasmid

   #177984

   Purpose

   All-in-one dox-inducible expression of Alternative Oxidase

   Depositor

   David Sabatini, Jessica Spinelli

   Article

   Spinelli et al Science. 2021 Dec 3;374(6572):1227-1237.

   Insert

   AOX
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Codon optimized cDNA for expression in human cells

   Promoter

   TRE

   Available Since

   Dec. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. Flag-GFP-HRP-TM_pLX304

    Plasmid

    #202021

    Purpose

    expresses GFP-HRP anchored to the extracellular side of the plasma membrane, lentiviral vector

    Depositor

    Alice Ting

    Article

    Qin et al Cell. 2023 Jun 23:S0092-8674(23)00596-2.

    Insert

    Flag-GFP-HRP-TM
    

    Use

    Lentiviral

    Tags

    Flag and GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. NeonGreen

    Plasmid

    #197924

    Purpose

    Monomer expression vector

    Depositor

    Salvatore Chiantia

    Article

    Petrich et al PLoS One. 2023 Aug 3;18(8):e0285486. doi

    Insert

    NeonGreen
    

    Expression

    Mammalian

    Mutation

    none

    Available Since

    April 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. Mouse GeCKO Lentiviral sgRNA Library (LentiCRISPR)

    Pooled Library

    #1000000052

    Purpose

    CRISPR gRNA knockout pooled library for targeting the mouse genome

    Depositor

    Feng Zhang

    Available Since

    July 23, 2014

    Availability

    Academic Institutions and Nonprofits only

13. GfaABC1D-DIO-mNeonGreen-mScarlet-l-WPRE

    Plasmid

    #223668

    Purpose

    Cre-dependently expresses mNeonGreen, mScarlet-I in astrocytes

    Depositor

    Jun Nagai

    Article

    Dewa et al Nature. 2025 Oct 15. doi: 10.1038/s41586

    Insert

    mNeonGreen-mScarlet-l
    

    Use

    AAV and Cre/Lox

    Promoter

    GfaABC1D

    Available Since

    Oct. 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLenti-HA-CHAC1

    Plasmid

    #242710

    Purpose

    human CHAC1 gene expression

    Depositor

    Xingguo Zhu

    Insert

    CHAC1 (CHAC1 Human)
    

    Use

    Lentiviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Multiple wobble mutations

    Promoter

    CMV

    Available Since

    Oct. 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. VEE-mScarlet3-IRES-E3-NSs-L*-EGFP-IRES-srIκBα-Smad7-SOCS1

    Plasmid

    #242412

    Purpose

    Self-amplifying RNA (saRNA) construct expresses dsRNA pathway inhibitors (E3, NSs, L*) and inflammatory signaling inhibitors, srIκBα, Smad7, and SOCS1.

    Depositor

    Ewan St. John Smith

    Article

    Lim et al bioRxiv 2024.09.24.614636

    Inserts

    mScarlet3
    IRES-E3-NSs-L*-EGFP
    IRES-srIκBα-Smad7-SOCS1
    

    Use

    Mouse Targeting; Self-amplifying rna

    Expression

    Mammalian

    Available Since

    Sept. 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. scAAV-hSyn-Cre

    Plasmid

    #138253

    Purpose

    A self-complementary AAV vector expresses Cre recombinase from hSyn promoter.

    Depositor

    Li Zhang

    Article

    Zingg et al J Neurosci. 2020 Apr 15;40(16):3250-3267

    Insert

    Cre recombinase
    

    Use

    AAV and Cre/Lox

    Expression

    Mammalian

    Promoter

    hSyn

    Available Since

    July 29, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV gfaABC1D:mScarlet-I3

    Plasmid

    #239684

    Purpose

    mScarlet-I3 expressed under an astrocyte-specific promoter.

    Depositor

    Marianne Fyhn

    Insert

    mScarlet-I3
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    gfaABC1D

    Available Since

    July 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. P2RX7-LgBiT(TK) Vector

    Plasmid

    #237101

    Purpose

    Express P2RX7-LgBiT Fusion Protein in Mammalian Cells under a TK promoter

    Depositor

    Promega Corporation

    Has Service

    DNA

    Insert

    P2RX7 (P2RX7 Human)
    

    Tags

    LgBiT

    Expression

    Mammalian

    Mutation

    None

    Promoter

    TK

    Available Since

    July 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. NanoLuc-MALT1 Vector

    Plasmid

    #236936

    Purpose

    Express NanoLuc(R)-MALT1 Fusion Protein in Mammalian Cells under a CMV promoter

    Depositor

    Promega Corporation

    Has Service

    DNA

    Insert

    MALT1 (MALT1 Human)
    

    Tags

    NanoLuc (R)

    Expression

    Mammalian

    Mutation

    None

    Promoter

    CMV

    Available Since

    July 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. pNL [NlucP/SRF/Hygro] Vector

    Plasmid

    #236835

    Purpose

    Express SRF response element in luciferase reporter assay

    Depositor

    Promega Corporation

    Has Service

    DNA

    Insert

    SRF (SRF Human)
    

    Use

    Luciferase

    Tags

    NanoLuc (R)

    Mutation

    None

    Promoter

    minP

    Available Since

    July 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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21. IRF5-NanoLuc Fusion Vector

    Plasmid

    #236792

    Purpose

    Express IRF5-NanoLuc(R) Fusion Protein in Mammalian Cells under a CMV promoter

    Depositor

    Promega Corporation

    Has Service

    DNA

    Insert

    IRF5 (IRF5 Human)
    

    Tags

    NanoLuc (R)

    Expression

    Mammalian

    Mutation

    None

    Promoter

    CMV

    Available Since

    July 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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22. pNL3.2.CMV.NLuc-PEST.Hygro

    Plasmid

    #209998

    Purpose

    In mammalian cells expresses NanoLuc mRNA with no TH as a control. Also expresses a hygromycin resistance marker.

    Depositor

    Kevin Weeks

    Article

    Hatfield et al Biochemistry. 2025 Sep 16;64(18):3854-38

    Inserts

    NanoLuc
    Hygromycin resistance marker
    

    Expression

    Mammalian

    Promoter

    CMV and SV40

    Available Since

    July 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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23. pEF.DEST51-ANXA6-mScarlet

    Plasmid

    #215475

    Purpose

    Expression vector with Annexin A6 tagged with mScarlet

    Depositor

    Johanna Ivaska

    Article

    Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

    Insert

    ANXA6 (ANXA6 Human)
    

    Tags

    mScarlet

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    May 29, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

24. pMXs-HRAS(G12V)-IRES-SNAP-p53DD

    Plasmid

    #233200

    Purpose

    Retroviral expression of HRAS(G12V) and SNAP-p53DD

    Depositor

    Kate Galloway

    Article

    Wang et al Cell Syst. 2025 Mar 10:101206. doi: 10.1

    Insert

    HRAS(G12V)-IRES-SNAP-p53DD (Trp53, HRAS Human, Mouse)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    May 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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25. pMXs-SNAP-p53DD-IRES-HRAS(G12V)

    Plasmid

    #233198

    Purpose

    Retroviral expression of SNAP-p53DD and HRAS(G12V)

    Depositor

    Kate Galloway

    Article

    Wang et al Cell Syst. 2025 Mar 10:101206. doi: 10.1

    Insert

    SNAP-p53DD-IRES-HRAS(G12V) (HRAS, Trp53 Human, Mouse)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    March 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pAIP4_3RZY

    Plasmid

    #234156

    Purpose

    Heterologous protein expression of fatty acid-binding protein, adipocyte (ALBP) in Escherichia coli

    Depositor

    Christopher Bahl

    Insert

    3RZY (FABP4 Human)
    

    Tags

    10x HisTag-Smt3

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 6, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

27. pGRACE

    Plasmid

    #230910

    Purpose

    Tracing of CRISPR activity in whole animals.

    Depositor

    Michael Boutros

    Article

    Port et al bioRxiv 2024.11.26.625385

    Insert

    GFP
    

    Use

    CRISPR

    Expression

    Insect

    Available Since

    Feb. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. Str-Endo-KKXX_SBP-EGFP-LIMP2

    Plasmid

    #222323

    Purpose

    Synchronize the trafficking of LIMP2 from the ER.

    Depositor

    Franck Perez

    Article

    Ecard et al Mol Biol Cell. 2024 Mar 1;35(3):ar42. do

    Insert

    Streptavidin-KDEL and LIMP2 fused to SBP-EGFP (SCARB2 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. Is3xP3-mCherry

    Plasmid

    #220091

    Purpose

    Expresses in ISE6 (Ixodes scapularis) cells, heat inducible. Promoter consists of 3 Pax 6 sites from 3xP3 with a minimal IsHSP70 promoter. Plasmid backbone derived from pGL-4.79 [Rluc] (Promega)

    Depositor

    Monika Gulia-Nuss

    Article

    Pham et al iScience. 2024 Jul 8;27(8):110468. doi:

    Insert

    3 Pax6 binding sites from 3xP3 with minimal HSP70 promoter from Ixodes scapularis
    

    Promoter

    Is3xP3

    Available Since

    Jan. 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pGL4_NFAT-RE-CMVmin-BC0524-luc2

    Plasmid

    #227139

    Purpose

    Barcoded assay, Ca2+ sensor; barcode BC0524

    Depositor

    Michael Wehr

    Article

    Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

    Insert

    6x clustered NFAT element linked to CMV minimal promoter driving barcode 0524 and a luciferase reporter gene
    

    Expression

    Mammalian

    Available Since

    Nov. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pX552-hsyn DIO GCaMP8f(with gRNA scaffold)

    Plasmid

    #199580

    Purpose

    Expresses GCaMP8f in a Cre-dependent manner

    Depositor

    Bryan Copits

    Article

    Moffa et al eNeuro. 2024 Jul 5;11(7):ENEURO.0438-23.

    Insert

    His-DIO GCaMP8f
    

    Use

    AAV, CRISPR, and Cre/Lox

    Tags

    6xHis

    Expression

    Mammalian

    Promoter

    hsyn

    Available Since

    Oct. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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32. pscALPSpuro-MDA5-S88D

    Plasmid

    #174226

    Purpose

    Expresses human MDA5 S88D mutant

    Depositor

    Jeremy Luban

    Article

    Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

    Insert

    MDA5 (IFIH1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    S88D

    Promoter

    SFFV

    Available Since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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33. CMV-IgK-pHluorin-TM-mRuby

    Plasmid

    #185546

    Purpose

    Expresses surface-localized pHluorin fused to intracellular expression of mRuby under CMV promoter

    Depositor

    Jaime de Juan-Sanz

    Article

    Cuhadar et al Cell Rep. 2024 May 28;43(5):114186. doi:

    Insert

    IgK-pHluorin-TM-mRuby
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. MTNR1B-DuET

    Plasmid

    #213349

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    MTNR1B (MTNR1B Human)
    

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Promoter

    CMV, T7

    Available Since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. CMKLR1-DuET

    Plasmid

    #213212

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    CMKLR1 (CMKLR1 Human)
    

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Promoter

    CMV, T7

    Available Since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pFBOH-MHL_CDC20:161-477

    Plasmid

    #210943

    Purpose

    Insect expression of human CDC20 fragment S161 to P477. N-terminal 6X His tag with TEV cleavage.

    Depositor

    Cheryl Arrowsmith

    Article

    Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

    Insert

    CDC20:S161-P477 (CDC20 Human)
    

    Tags

    MHHHHHHSSGRENLYFQG

    Expression

    Insect

    Mutation

    wild type

    Promoter

    Polyhedrin

    Available Since

    March 19, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

37. pNLF1-C_CDC20:1-499

    Plasmid

    #211062

    Purpose

    Mammalian expression of full-length human CDC20. C-terminal NanoLuc luciferase tag.

    Depositor

    Cheryl Arrowsmith

    Article

    Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

    Insert

    CDC20:M1-R499 (CDC20 Human)
    

    Tags

    NanoLuc luciferase

    Expression

    Mammalian

    Mutation

    wild type

    Promoter

    CMV

    Available Since

    March 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pNLF1-N_CDC20:1-499

    Plasmid

    #211058

    Purpose

    Mammalian expression of full-length human CDC20. N-terminal NanoLuc luciferase tag.

    Depositor

    Cheryl Arrowsmith

    Article

    Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

    Insert

    CDC20:M1-R499 (CDC20 Human)
    

    Tags

    NanoLuc luciferase

    Expression

    Mammalian

    Mutation

    wild type

    Promoter

    CMV

    Available Since

    March 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pBiT3.1-N_CDC20:1-499

    Plasmid

    #211122

    Purpose

    Mammalian expression of full-length human CDC20. N-terminal HiBiT tag.

    Depositor

    Cheryl Arrowsmith

    Article

    Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

    Insert

    CDC20:M1-R499 (CDC20 Human)
    

    Tags

    MVSGWRLFKKISGSSGGSSG

    Expression

    Mammalian

    Mutation

    wild type

    Promoter

    CMV

    Available Since

    March 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pBiT3.1-C_CDC20:1-499

    Plasmid

    #211163

    Purpose

    Mammalian expression of full-length human CDC20. C-terminal HiBiT tag.

    Depositor

    Cheryl Arrowsmith

    Article

    Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

    Insert

    CDC20:M1-R499 (CDC20 Human)
    

    Tags

    GSSGGSSGVSGWRLFKKIS

    Expression

    Mammalian

    Mutation

    wild type

    Promoter

    CMV

    Available Since

    March 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge