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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 12,282 results for: LEA

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Showing: 4181 - 4200 of 12282 results

  1. Lenti-pNFkB::Cre-mRuby3

    Plasmid
    #164137
    Purpose
    Lentiviral construct for building stable cell lines expressing Cre recombinase and mRuby3 in the presence of TNFa
    Depositor
    Hyongbum Kim
    Publication
    Park et al Cell. 2021 Jan 28. pii: S0092-8674(21)00
    Insert
    NFkB promoter-Cre-T2A-mRuby3
    Use
    CRISPR, Cre/Lox, Lentiviral, and Synthetic Biolog…
    Expression
    Mammalian
    Promoter
    NFkB synthetic promoter
    Available since
    Feb. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    164137-plasmid-map-sequence-id-323778

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  2. pGBW-m4134552

    Plasmid
    #152652
    Purpose
    Mammalian expression plasmid for SARS-CoV-2 N (nucleocapsid)
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SARS-CoV-2 N (nucleocapsid) (N Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;3xFlag
    Expression
    Mammalian
    Mutation
    wt
    Promoter
    CMV
    Available since
    July 30, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    152652-plasmid-map-sequence-id-320770

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  3. pGBW-m4134371

    Plasmid
    #152394
    Purpose
    Mammalian expression plasmid for SARS-CoV-2 M (membrane)
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SARS-CoV-2 M (membrane) (M Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;3xFlag
    Expression
    Mammalian
    Mutation
    wt
    Promoter
    CMV
    Available since
    July 30, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    152394-plasmid-map-sequence-id-310887

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  4. pCEP4-AD8gp160

    Plasmid
    #123260
    Purpose
    Mammalian expression plasmid for Env from the AD8 HIV-1 isolate
    Depositor
    Erik Procko
    Publication
    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    HIV-1 (AD8) Env
    Tags
    CD5 leader peptide
    Expression
    Mammalian
    Mutation
    Codon-optimized synthetic gene
    Promoter
    CMV
    Available since
    April 1, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    123260-plasmid-map-sequence-id-235225

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  7. TRIM33 (TRIM33B-c100)

    Plasmid
    #98243
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    TRIM33B (TRIM33 Human)
    Tags
    His-6-TEV
    Expression
    Bacterial
    Mutation
    pfam00628:PHD 889-932, pfam00439:Bromodomain 963-…
    Promoter
    T7
    Available since
    Sept. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    98243-plasmid-map-sequence-id-193421

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  8. pBAD mApple

    Plasmid
    #91772
    Purpose
    Bacterial expression of a fluorescent protein, monomeric Apple (mApple). Note: Addgene identified several mutations compared to the final published sequence.
    Depositor
    Erik Rodriguez, Roger Tsien
    Publication
    Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:
    Insert
    monomeric Apple
    Tags
    Hexa-Histidine tag, Xpress epitope for detection …
    Expression
    Bacterial
    Mutation
    R97K, E152S, A180S, V207T compared to mApple (FP …
    Promoter
    araBAD
    Available since
    June 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    91772-plasmid-map-sequence-id-181643

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  9. pEGFP-ninein N-ter

    Plasmid
    #73520
    Purpose
    Encodes the N-terminus of mouse ninein (residues 1-373) with an EGFP tag at its N-terminus
    Depositor
    Michel Bornens
    Publication
    Delgehyr et al J Cell Sci. 2005 Apr 15;118(Pt 8):1565-7
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ninein N-ter (residues 1-373) (Nin Mouse)
    Tags
    EGFP tag
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    April 29, 2016
    Availability
    Academic Institutions and Nonprofits only

  10. pmalc4x_E323K_TEV

    Plasmid
    #47699
    Purpose
    Expresses MBP myocilin olfactomedin domain fusion protein with a TEV protease recognition sequence and the mutation E323K
    Depositor
    Raquel Lieberman
    Publication
    Burns et al Biochemistry. 2011 Jul 5;50(26):5824-33.
    Insert
    Maltose Binding Protein and Olfactomedin-like domain with TEV cleavage site (MYOC Human)
    Tags
    MBP
    Expression
    Bacterial
    Mutation
    changed glutamic acid 323 to lysine
    Promoter
    Ptac
    Available since
    Sept. 9, 2013
    Availability
    Industry, Academic Institutions, and Nonprofits
    47699-plasmid-map-sequence-id-68104

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  13. pGBW-m4133639

    Plasmid
    #152344
    Purpose
    Mammalian expression plasmid for SARS-CoV-2 3C-like proteinase
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SARS-CoV-2 3C-like proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;3xFlag
    Expression
    Mammalian
    Mutation
    codon optimized
    Promoter
    CMV
    Available since
    June 17, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    152344-plasmid-map-sequence-id-300328

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  14. pcDNA3.1(+)-ExRai-AKAR(T/A)

    Plasmid
    #118406
    Purpose
    Negative-control mutant for ExRai-AKAR biosensor.
    Depositor
    Jin Zhang
    Publication
    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ExRai-AKAR(T/A)
    Tags
    6xHis, T7 tag (gene 10 leader), and Xpress (TM) t…
    Expression
    Mammalian
    Mutation
    Contains Thr-to-Ala mutation in substrate sequenc…
    Promoter
    CMV
    Available since
    April 25, 2019
    Availability
    Academic Institutions and Nonprofits only
    118406-plasmid-map-sequence-id-242821

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  18. pcDNA3-pmEMBer

    Plasmid
    #174441
    Purpose
    Plasma membrane-targeted ERK monobody binder for local inhibition of ERK activity in live cells.
    Depositor
    Jin Zhang
    Publication
    Keyes et al Elife. 2020 May 26;9. pii: 57410. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    pmEMBer
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Aug. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    174441-plasmid-map-sequence-id-344972

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  19. pAAV-SynI-CreOn-FlpOff-Kir2.1MUT-P2A-EGFP

    Plasmid
    #176279
    Purpose
    Viral vector for co-expression of non-functional Kir2.1 and EGFP in cells expressing Cre AND NOT Flp driven by the human Synapsin promoter.
    Depositor
    Martin Riccomagno
    Publication
    Ubina et al J Cell Sci. 2021 Oct 15;134(20). pii: 27
    Insert
    Kir2.1MUT-P2A-EGFP (Kcnj2 Synthetic, Mouse)
    Use
    AAV and Cre/Lox
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    GYG to AAA (aa144-146)
    Promoter
    human Synapsin I
    Available since
    Jan. 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    176279-plasmid-map-sequence-id-350684

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Showing: 4181 - 4200 of 12282 results