We narrowed to 15,789 results for: LEA

1. pcDNA3.1-subtilase A

   Plasmid

   #35948

   Depositor

   Zhaoyang Feng

   Article

   Yuan et al PLoS One. 2011;6(8):e22354. Epub 2011 Au

   Insert

   Subtilase A (subA E. Coli)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 14, 2012

   Availability

   Academic Institutions and Nonprofits only

2. pTORKm43GWR

   Plasmid

   #112900

   Purpose

   Multisite (attR3/attR4) Gateway vector for expression in oomycetes. Confers resistance to geneticin (G418). Contains additional cassette for constitutive expression of tdTomato.

   Depositor

   Sebastian Schornack

   Article

   Evangelisti et al MBio. 2019 Oct 1;10(5). pii: mBio.01516-

   Type

   Empty backbone

   Use

   Oomycete expression

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAAV-FLEX-HM4Di-YFP

   Plasmid

   #209193

   Purpose

   Cre-dependent expression of Hm4Di fused to YFP; Chemogenetics

   Depositor

   Larry Zweifel

   Article

   Sanford et al Neuron. 2017 Jan 4;93(1):164-178. doi: 1

   Insert

   Hm4Di
   

   Use

   AAV

   Tags

   YFP

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   June 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pGEX-GST-AGO1

   Plasmid

   #24316

   Depositor

   Carl Novina

   Article

   Wang et al Nat Struct Mol Biol. 2009 Dec . 16(12):1

   Insert

   human AGO1 (AGO1 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 7, 2010

   Availability

   Academic Institutions and Nonprofits only

5. pSCL.139

   Plasmid

   #184994

   Purpose

   Express Cas9-P2A-Eco1RT in human cells

   Depositor

   Seth Shipman

   Article

   Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

   Insert

   SpCas9-P2A-Eco1RT
   

   Use

   Tags

   SV40NLS

   Expression

   Mammalian

   Mutation

   Human codon optimized RT

   Promoter

   TetOn-3g

   Available since

   Nov. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCMV HDM2(C464A)

   Plasmid

   #12086

   Depositor

   Tyler Jacks

   Article

   Boyd et al Nat Cell Biol. 2000 Sep . 2(9):563-8.

   Insert

   HDM2 (MDM2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   C464A: RING-finger-domain mutant

   Promoter

   Available since

   June 8, 2006

   Availability

   Academic Institutions and Nonprofits only

7. pcDNA3.1 HA-rnSOX9

   Plasmid

   #62972

   Purpose

   Expresses HA-tagged rat SOX9

   Depositor

   Michael Ruppert

   Article

   Deng et al J Cell Sci. 2015 Jan 27. pii: jcs.162164

   Insert

   SOX9 (Sox9 Rat)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 28, 2015

   Availability

   Academic Institutions and Nonprofits only

8. pDONR223_BIRC6

   Plasmid

   #197974

   Purpose

   Entry clone for wt BIRC6

   Depositor

   Eric Fischer

   Article

   Hunkeler et al Science. 2023 Mar 17;379(6637):1105-1111

   Insert

   BIRC6 (BIRC6 Human)
   

   Use

   Other

   Tags

   Expression

   Mutation

   L1332V

   Promoter

   Available since

   March 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pSV-PGC1

   Plasmid

   #3

   Depositor

   Bruce Spiegelman

   Article

   Puigserver et al Cell 1998 Mar 20;92(6):829-39.

   Insert

   PGC-1 alpha (Ppargc1a Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 23, 2006

   Availability

   Academic Institutions and Nonprofits only

10. pcDNA-LBR-mRFP1-polyA

    Plasmid

    #59749

    Purpose

    Express LBR-mRFP1 in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes. This LBR contains N-terminal domain only.

    Depositor

    Yi Zhang

    Article

    Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

    Insert

    LBR-mRFP1
    

    Use

    Tags

    mRFP1

    Expression

    Mammalian

    Mutation

    aa 1-249 of LBR

    Promoter

    CMV and T7

    Available since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pVP2A-PIF6

    Plasmid

    #73369

    Purpose

    Expresses Adeno-associated virus VP2 with PIF6 insert.

    Depositor

    Junghae Suh

    Article

    Gomez et al ACS Nano. 2015 Nov 30.

    Insert

    PIF6 (PIL2 Mustard Weed)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pmKalama1-Nuc

    Plasmid

    #14894

    Purpose

    Mammalian expression of mKalama1 blue fluorescent protein with NLS

    Depositor

    Robert Campbell

    Article

    Ai et al Biochemistry. 2007 May 22;46(20):5904-10

    Insert

    pmKalama1-Nuc
    

    Use

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    I178T mismatch, replace EYFP of the vector pEYFP-…

    Promoter

    Available since

    May 15, 2007

    Availability

    Academic Institutions and Nonprofits only

13. His-DERA

    Plasmid

    #203225

    Purpose

    For protein expression. E. coli 2-deoxy-D-ribose-5-phosphate aldolase. Converts dR5P to G3P and acetaldehyde. Pet28a plasmid, KAN resistant, N-terminal TEV-cleavable 6xHis tag.

    Depositor

    Vern Schramm

    Article

    Wagner et al Anal Biochem. 2023 Jul 1;672:115171. doi

    Insert

    deoxyribose aldolase
    

    Use

    Tags

    N-terminal TEV cleavable 6xHis tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pTRE2-Bla(HA–RILP)

    Plasmid

    #102425

    Purpose

    HA tag fused to the N-terminus of RILP for the expression in mammalian cells.

    Depositor

    Steven Weinman

    Article

    Wozniak et al Proc Natl Acad Sci U S A. 2016 Nov 1;113

    Insert

    Rab interacting lysosomal protein (RILP Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Tet-responsive

    Available since

    Oct. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET26b-Granzyme A (S-A)

    Plasmid

    #8825

    Depositor

    Judy Lieberman

    Article

    Beresford et al Proc Natl Acad Sci U S A 1997 Aug 19;94(

    Insert

    Human Granzyme A inactive (GZMA Human)
    

    Use

    Tags

    (His)6

    Expression

    Bacterial

    Mutation

    changed Serine 184 to Alanine

    Promoter

    Available since

    June 22, 2006

    Availability

    Academic Institutions and Nonprofits only

16. pcDNA3-ABKAR(T/A)

    Plasmid

    #140052

    Purpose

    Negative control mutant for ABKAR biosensor.

    Depositor

    Jin Zhang

    Article

    Sample et al Mol Biol Cell. 2015 May 15;26(10):1935-4

    Insert

    ABKAR(T/A)
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    Target Thr residue in substrate domain mutated to…

    Promoter

    CMV

    Available since

    Nov. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. MSP1594

    Plasmid

    #65775

    Purpose

    Human expression vector for S. thermophilus 1 Cas9: CAG-humanSt1Cas9-NLS

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

    Insert

    mammalian codon-optimized Streptococcus thermophilus1 Cas9-NLS
    

    Use

    CRISPR

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pEJS2712-CMV-eNme2-C-ABE8e-i1-L10

    Plasmid

    #228733

    Purpose

    Expresses domain inlaid eNme2-C nCas9 (D16A)-i1-ABE8e base editor with linker-10 in mammalian cells

    Depositor

    Erik Sontheimer

    Article

    Bamidele et al bioRxiv November 07, 2024

    Insert

    Domain inlaid eNme-C nCas9 (D16A)-i1-L10
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pOCC7

    Plasmid

    #118876

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal HIS6 tag, cleavable with 3C

    Depositor

    PEPC MPI CBG, Marc Bickle

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-NotI-ccdB-AscI-3C-HIS6-stop-HindIII cassette
    

    Use

    Tags

    HIS6, cleavable with 3C protease

    Expression

    Insect

    Mutation

    Promoter

    polH

    Available since

    Jan. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pPM1001

    Plasmid

    #167548

    Purpose

    Encodes genes that confer expression of Vibrio cholerae O antigens of the Inaba serotype in E. coli K-12

    Depositor

    Renato Morona

    Article

    Manning et al Infect Immun. 1986 Aug;53(2):272-7. doi:

    Insert

    Vibrio cholerae O antigens genes of the Inaba serotype
    

    Use

    Cosmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge