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pSAM2_mCherry_Gata6

Plasmid

#72694

Purpose

Doxycyline regulated expression of Gata6 in mammalian cells

Depositor

Timothy Kamp

Publication

Lalit et al Cell Stem Cell. 2016 Mar 3;18(3):354-67.

Insert

Gata 6 (Gata6 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV-Doxycycline

Available since

Oct. 17, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pCAG Cas9-2A-Citrine

Plasmid

#92358

Purpose

CAG-driven ubiquitous expression of Cas9. Contains 2A-Citrine reporter. For CRISPR mediated gene knockouts in chicken embryos.

Depositor

Tatjana Sauka-Spengler

Publication

Williams et al Development. 2018 Feb 23;145(4). pii: de

Insert

Cas9 2A Citrine

Expression

Mammalian

Available since

Aug. 24, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pGRG36-Kn-PA1-GFP

Plasmid

#79088

Purpose

pGRG36-Kn-PA1-GFP is a Kanamycine resistance plasmid used to integrate a GFP expression cassette into the chromosome of E.coli, described in Yang et al. 2016 (Pending publication Pubmed ID)

Depositor

Frank Rosenzweig

Publication

Yang et al Appl Environ Microbiol. 2020 Apr 1;86(8)

Insert

GFP

Use

Bacterial chromosome integration and expression o…

Expression

Bacterial

Promoter

PA1

Available since

July 12, 2017

Availability

Academic Institutions and Nonprofits only

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pLX_TRC311 ETV1

Plasmid

#74981

Purpose

Constitutive ETV1 ORF, blasticidin selection

Depositor

William Hahn

Publication

Wang et al Cell Rep. 2017 Feb 7;18(6):1543-1557. do

Insert

ETV1 (ETV1 Human)

Expression

Mammalian

Promoter

EF1a

Available since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pY31

Plasmid

#84746

Purpose

Expresses huLbCpf1-P2A-puro and crRNA guide

Depositor

Feng Zhang

Publication

Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

Inserts

huLbCpf1
puromycin resistance gene

Use

CRISPR

Tags

3xHA, NLS, and T2A

Expression

Mammalian

Promoter

CMV

Available since

Nov. 7, 2016

Availability

Academic Institutions and Nonprofits only

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h-beta-ENaC

Plasmid

#83429

Purpose

full length human beta ENaC with V5 tag

Depositor

Christie Thomas

Publication

Raikwar et al Am J Physiol Renal Physiol. 2008 May;294

Insert

human beta ENaC (SCNN1B Human)

Tags

V5

Expression

Mammalian

Promoter

CMV

Available since

Nov. 1, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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GUM BUB3

Plasmid

#84029

Purpose

Expression of mCherry tagged BUB3 in human cells

Depositor

Patrick Paddison

Publication

Toledo et al Dev Cell. 2014 Feb 10;28(3):282-94. doi:

Insert

BUB3 (BUB3 Human)

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Promoter

UCOE / SFFV

Available since

Oct. 13, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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Tet-CA-Src-GFP

Plasmid

#83469

Purpose

Lentiviral vector for doxycycline-inducible expression of constitutively active c-Src in mammalian cells

Depositor

Barry Gumbiner

Publication

Kim et al J Cell Biol. 2015 Aug 3;210(3):503-15. d

Insert

Src (SRC Chicken)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

Y527F (constitutively active)

Promoter

doxycycline-inducible promoter

Available since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pT-GEM(0)

Plasmid

#62891

Purpose

Trojan-Gal4 Expression Module in Phase 0. Insert T2A-Gal4 to genomic loci using the Crispr/Cas technology. Can be converted by cassette exchange into other Trojan exons

Depositor

Benjamin White

Publication

Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

Type

Empty backbone

Available since

July 2, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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Notch1 intracellular domain-pcw107

Plasmid

#64621

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Publication

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

NOTCH1 (NOTCH1 Human)

Use

Lentiviral

Mutation

intracellular domain *

Promoter

PGK

Available since

May 12, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles
pJZC78

Plasmid

#62339

Purpose

sgRNA + 1x COM with COM-KRAB effector for mammalian cells

Depositor

Wendell Lim, Stanley Qi

Publication

Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

Inserts

sgRNA + 1x COM binding module
COM-KRAB

Use

Lentiviral

Tags

KRAB

Expression

Mammalian

Mutation

Targets sv40 promoter, sequence: CATACTTCTGCCTGCT…

Promoter

CMV and U6

Available since

April 13, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCMU-ERr

Plasmid

#61170

Purpose

Fluorescent reporter for endoplasmic reticulum AtWAK2sp-mCherry-HDEL expressed under AtUBQ10 promoter

Depositor

Maria Harrison

Publication

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

AtWAK2-mCherry-HDEL

Tags

mCherry

Expression

Plant

Promoter

AtUBQ10

Available since

April 9, 2015

Availability

Academic Institutions and Nonprofits only

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pLX304-YAP1 _TA

Plasmid

#59143

Purpose

YAP1 mutants that harbor a deletion of the transcriptional activation domain

Depositor

William Hahn

Publication

Shao et al Cell. 2014 Jul 3;158(1):171-84. doi: 10.

Insert

YAP1 (YAP1 Human)

Use

Lentiviral

Mutation

TA (transactivation) domain deleted; aa291-497

Promoter

CMV

Available since

Oct. 23, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pKTrfp

Plasmid

#59772

Purpose

pKT230 derivative; PBAD_rfp; cmR

Depositor

Nathan Hillson

Publication

Bi et al Microb Cell Fact. 2013 Nov 13;12:107. do

Insert

rfp

Expression

Bacterial

Promoter

pBAD

Available since

Sept. 26, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDESTN-MTp-p53-Venus

Plasmid

#53736

Purpose

Expresses Venus tagged p53 under the metallothionein promoter

Depositor

Galit Lahav

Publication

Batchelor et al Mol Cell. 2008 May 9;30(3):277-89. doi:

Insert

p53 (TP53 Human)

Tags

Venus

Expression

Mammalian

Promoter

metallothionein promoter

Available since

June 16, 2014

Availability

Academic Institutions and Nonprofits only

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pFRT-TODestFLAGHAhTTP

Plasmid

#48738

Purpose

Expresses hTTP

Depositor

Thomas Tuschl

Insert

TTP (ZFP36 Human)

Tags

FLAG, HA, and V5

Expression

Mammalian

Promoter

CMV, 2x Tet

Available since

June 13, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pENTR-NICD1

Plasmid

#46048

Depositor

Xin Chen

Publication

Fan et al J Clin Invest. 2012 Aug 1;122(8):2911-5.

Insert

Notch1 (C-term) (Notch1 Mouse)

Use

Gateway Cloning

Tags

Myc tag

Mutation

Cytoplasmic domain initiating at amino acid 1753

Available since

July 2, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles
Active Wnt11-V5

Plasmid

#43824

Depositor

Xi He

Publication

MacDonald et al J Biol Chem. 2014 May 19. pii: jbc.M114.

Insert

human WNT11 (WNT11 Human)

Tags

V5

Expression

Mammalian

Promoter

CMV

Available since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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TXNRD1

Plasmid

#38863

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

TXNRD1 (Txnrd1 Human)

Tags

His Tev

Expression

Bacterial

Available since

March 18, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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Mek2

Plasmid

#40776

Depositor

Dustin Maly

Insert

MKK2 (MAP2K2 Human)

Tags

Myc

Expression

Mammalian

Mutation

Starting Met removed

Promoter

CMV

Available since

Oct. 15, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles