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We narrowed to 63,812 results for: cloning

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Showing: 4181 - 4200 of 63812 results

  1. pLIX403_Capture1_APOBEC_HA_P2A_mRuby

    Plasmid
    #183901
    Purpose
    Inducible lentiviral expression, TRE-gateway-APOBEC-HA-P2A-mRuby; PGK-puro-2A-rtTA
    Depositor
    Eugene Yeo
    Publication
    Brannan et al Nat Methods. 2021 May;18(5):507-519. doi
    Type
    Empty backbone
    Use
    Lentiviral; Gateway destination vector, doxycycli…
    Tags
    Capture1_APOBEC_HA_P2A_mRuby
    Expression
    Mammalian
    Promoter
    TRE promoter, Tet ON
    Available since
    Oct. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    183901-plasmid-map-sequence-id-374684

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  4. pCMV-T7-ABE8e-nSpCas9-NG-P2A-EGFP (KAC999)

    Plasmid
    #185914
    Purpose
    pCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nickase SpCas9-NG(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized ABE8e-nSpCas9-NG-P2A-EGFP
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS and BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpCas9-NG=D10A/L1111R/D1135V/G1218R/E1219F/A1322…
    Promoter
    CMV and T7
    Available since
    July 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    185914-plasmid-map-sequence-id-367363

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  13. pCMV-SaPE2

    Plasmid
    #174817
    Purpose
    Mammalian expression of SaCas9 prime editor 2
    Depositor
    David Liu
    Publication
    Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SaPE2
    Tags
    SV40 bpNLS
    Expression
    Mammalian
    Mutation
    Detailed in manuscript
    Promoter
    CMV
    Available since
    Oct. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    174817-plasmid-map-sequence-id-343608

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  14. pH-nCas9-PPE-V2

    Plasmid
    #170131
    Purpose
    For plant prime editing in rice plants or monocotyledons protoplasts
    Depositor
    Caixia Gao
    Publication
    Lin et al Nat Biotechnol. 2021 Mar 25. pii: 10.103
    Insert
    nCas9(H840A)-M-MLV
    Use
    CRISPR
    Expression
    Plant
    Mutation
    H840A for Cas9; D200N, T306K, W313F, T330P and L…
    Promoter
    maize Ubiquitin-1, OsU3
    Available since
    May 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    170131-plasmid-map-sequence-id-333607

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  15. pMuLE_EXPR_CMV-eGFP_TOP-NLuc1.1_12GLI-FLuc_CBF-GLuc

    Plasmid
    #113862
    Purpose
    Triple pathway reporter, 3P-Luc; wnt-NLuc1.1, hedgehog-FLuc, notch-GLuc; plus CMV-eGFP. Gateway expression vector for lentivirus generation.
    Depositor
    Manfred Ogris
    Publication
    Maier et al PLoS One. 2019 Dec 20;14(12):e0226570. d
    Inserts
    CMV-eGFP
    TOP-NLuc1.1
    12GLI-Fluc
    CBF-GLuc
    Use
    Lentiviral
    Expression
    Mammalian
    Available since
    May 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    113862-plasmid-map-sequence-id-328796

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  19. JD690

    Plasmid
    #160397
    Purpose
    Binary vector pGWB14 including citrus GRF4-GIF1 under 35S promoter/ Plant transformation
    Depositor
    Jorge Dubcovsky
    Publication
    Debernardi et al Nat Biotechnol. 2020 Oct 12. pii: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    35S::citrus GRF4-GIF1 chimera
    Promoter
    35S
    Available since
    Nov. 30, 2020
    Availability
    Academic Institutions and Nonprofits only
    160397-plasmid-map-sequence-id-318344

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Showing: 4181 - 4200 of 63812 results