We narrowed to 13,164 results for: cdna

1. pcDNA3-HLA-cMyc-EcopT1R1

   Plasmid

   #113962

   Purpose

   mammalian expression plasmid for c-myc-tagged E. coli codon-optimized human T1R1 with signal peptide of HLA class I histocompatibility antigen A-2 alpha chain

   Depositor

   Erik Procko

   Article

   Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061

   Insert

   T1R1 (TAS1R1 Human)
   

   Use

   Tags

   Signal/leader sequence from HLA class I histocomp…

   Expression

   Mammalian

   Mutation

   truncate N-terminal 24 residues

   Promoter

   Available since

   Feb. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA5/FRT/TO-GAlphaz-RLuc8

   Plasmid

   #140978

   Purpose

   Encodes a G alpha subunit (GNAZ) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

   Depositor

   Bryan Roth

   Article

   Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

   Insert

   GAlphaz-Rluc8 (GNAZ Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   RLuc8 and flanking SGGGGS linkers have been inser…

   Promoter

   CMV

   Available since

   June 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-N-Flag-NLRP3 91-710

   Plasmid

   #75140

   Purpose

   Expresses N-terminal Flag-tagged amino acids 91-710 of NLRP3 (the nucleotide-binding domain) in mammalian cells

   Depositor

   Bruce Beutler

   Article

   Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

   Insert

   NLR family, pyrin domain containing 3 (Nlrp3 Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   fragment encoding amino acids 91-710

   Promoter

   CMV

   Available since

   May 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1+CMV-Ins-GLuc

   Plasmid

   #89929

   Purpose

   Expresses Insulin-GLuc from the CMV promoter. Gaussia Luc is inserted within the C-peptide and is co-secreted with insulin.

   Depositor

   Melanie Cobb

   Article

   Kalwat et al ACS Sens. 2016 Oct 28;1(10):1208-1212. E

   Insert

   human insulin-Gaussia-Luciferase (INS Human, Synthetic)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   DNA sequence for Gaussia luciferase was humanized…

   Promoter

   CMV

   Available since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1-FLAG-Dicer-delPAZ

   Plasmid

   #51367

   Depositor

   Narry Kim

   Article

   Lee et al EMBO J. 2006 Feb 8;25(3):522-32. Epub 20

   Insert

   Dicer (DICER1 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   From N terminus to PAZ domain deletion

   Promoter

   CMV

   Available since

   March 31, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1-FLAG-Dicer-delDEAD

   Plasmid

   #51364

   Depositor

   Narry Kim

   Article

   Lee et al EMBO J. 2006 Feb 8;25(3):522-32. Epub 20

   Insert

   Dicer (DICER1 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   From N terminus to DEAD domain deletion

   Promoter

   CMV

   Available since

   March 31, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1/Myc-His(-)-MSulf-2

   Plasmid

   #13008

   Depositor

   Steven Rosen

   Article

   Morimoto-Tomita et al J Biol Chem. 2002 Dec 20. 277(51):49175-

   Insert

   mouse sulfatase 2 (Sulf2 Mouse)
   

   Use

   Tags

   6xHis and Myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 30, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1-Clover-Prx2-mRuby2

   Plasmid

   #117906

   Purpose

   fluorescent reporter of cytosolic hydrogen peroxide

   Depositor

   Hadley Sikes

   Article

   Langford et al Nat Commun. 2018 Aug 7;9(1):3145. doi: 1

   Insert

   Clover-Prx2-mRuby2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1-FLAG-Dicer-delDUF

   Plasmid

   #51365

   Depositor

   Narry Kim

   Article

   Lee et al EMBO J. 2006 Feb 8;25(3):522-32. Epub 20

   Insert

   Dicer (DICER1 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   From N terminus to DUF domain deletion

   Promoter

   CMV

   Available since

   March 31, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3 MDM2 S166D S186D

    Plasmid

    #16236

    Depositor

    Mien-Chie Hung

    Article

    Zhou et al Nat Cell Biol. 2001 Nov . 3(11):973-82.

    Insert

    MDM2 (MDM2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Ser166 and Ser186 replaced by Asp

    Promoter

    Available since

    Nov. 30, 2007

    Availability

    Academic Institutions and Nonprofits only

11. pcDNA3-FLAG-Synaptojanin 1-170

    Plasmid

    #22292

    Depositor

    Pietro De Camilli

    Article

    Haffner et al FEBS Lett. 1997 Dec 15. 419(2-3):175-80.

    Insert

    Synaptojanin 1_170 (SYNJ1 Human)
    

    Use

    Tags

    Flag

    Expression

    Bacterial and Mammalian

    Mutation

    K334R compared to Genbank ID NM_003895

    Promoter

    Available since

    Oct. 9, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3-Venus-StagRFP-AktAR

    Plasmid

    #173020

    Purpose

    Yellow-red FRET-based Akt/PKB activity reporter for multiplexed imaging applications.

    Depositor

    Jin Zhang

    Article

    Mo et al Nat Commun. 2020 Apr 15;11(1):1848. doi:

    Insert

    Venus-StagRFP-AktAR
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1-N-Myc_1-205 USP7

    Plasmid

    #131245

    Purpose

    Mammalian expression of the USP7 TRAF-like domain with an N-terminal Myc tag.

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

    Insert

    Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Expresses a mutant form of our pcDNA3.1-N-Myc_1-5…

    Promoter

    Available since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1-MS2-HA-Hs4E-T_AA

    Plasmid

    #148233

    Purpose

    Mammalian Expression of Hs4E-T

    Depositor

    Elisa Izaurralde

    Insert

    Hs4E-T (EIF4ENIF1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1(+)/GUG-DAP5-3XFLAG

    Plasmid

    #127330

    Purpose

    Express 3xFLAG tagged DAP5 protein from GUG start codon

    Depositor

    Jeremy Wilusz

    Article

    Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

    Insert

    DAP5 (EIF4G2 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA5-FRT/TO-N-mEGFP-PARP7

    Plasmid

    #178004

    Purpose

    Expresses N-terminally mEGFP-tagged PARP7

    Depositor

    Karla Feijs

    Article

    Weixler et al Life Sci Alliance. 2022 Nov 11;6(1):e202

    Insert

    PARP7 (TIPARP Human)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. LARG mCherry sspB in pcDNA3.1

    Plasmid

    #90459

    Purpose

    RhoA mediated cell migration

    Depositor

    Narasimhan Gautam

    Article

    O'Neill et al Dev Cell. 2018 Jun 15. pii: S1534-5807(1

    Inserts

    LARG DH-PH (aa 766–1138) (ARHGEF12 Human)
    mCherry
    sspB
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R73Q

    Promoter

    CMV

    Available since

    June 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1/NDUFS7/myc-His

    Plasmid

    #127488

    Purpose

    Expresses myc-tagged NDUFS7 in mammalian cells

    Depositor

    Dieter Wolf, Shashi Jain

    Article

    Jain et al Cell Chem Biol. 2021 Nov 23. pii: S2451-

    Insert

    NDUFS7 (NDUFS7 Human)
    

    Use

    Tags

    myc-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3-FLAG-MTOR-I2500F

    Plasmid

    #69014

    Purpose

    activating MTOR mutation

    Depositor

    David Sabatini

    Article

    Grabiner et al Cancer Discov. 2014 May;4(5):554-63. doi

    Insert

    MTOR-I2500F (MTOR Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    change Isoleucine 2500 to Phenylalanine

    Promoter

    Available since

    Dec. 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA4-TO-Puromycin-mVenus-MAP

    Plasmid

    #44118

    Depositor

    Dannel McCollum

    Article

    Ma et al Mol Cell Proteomics. 2012 Aug;11(8):501-

    Type

    Empty backbone

    Use

    Tags

    mVenus-MAP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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