We narrowed to 19,393 results for: IRE
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Plasmid#153959PurposeUsed for EGxxFP assay with CD55 intron 3, exon 4, and intron 4 sequencesDepositorInsertCD55 (a 1,016-bp fragment from intron 3, exon 4, and intron 4)
UseA plasmid specific for egxxfp assayTagsN/AExpressionMammalianMutationNo mutationPromoterCAG promoterAvailable SinceDec. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAA2
Plasmid#154162PurposeFirefly luciferase under the control of 4 copies of: the enhancer for the Drosophila melanogaster Metchnikowin gene, 4 Rel1A-specific NF-_B sites, 3 GATA sites and 3 putative ecdysone-response elements, plus an additional NF-_B site.DepositorInsertFirefly luciferase
UseLuciferaseMutation16 wt NF-κB sites were replaced with Rel1A-specif…Available SinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAA1
Plasmid#154161PurposeFirefly luciferase under the control of 3 copies of: the enhancer for the Drosophila melanogaster Metchnikowin gene, 4 Rel1A-specific NF-_B sites, 3 GATA sites and 3 putative ecdysone-response elements, plus an additional NF-_B site.DepositorInsertFirefly luciferase
UseLuciferaseMutation12 wt NF-κB sites were replaced with Rel1A-specif…Available SinceAug. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKM62
Plasmid#154160PurposeFirefly luciferase under the control of the enhancer for the Drosophila melanogaster Metchnikowin gene, with 4 Rel2-specific (VII) NF-_B sites, 3 GATA sites and 3 putative ecdysone-response elements, plus an additional NF-_B site.DepositorInsertFirefly luciferase
UseLuciferaseMutation4 wt NF-κB sites were replaced with Rel2-specific…Available SinceAug. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKM59
Plasmid#154158PurposeFirefly luciferase under the control of the enhancer for the Drosophila melanogaster Metchnikowin gene, a TATA box and Kozak sequence of the Metchnikowin gene and additional NF-κB site.DepositorInsertFirefly luciferase
UseLuciferasePromoterEnhancer for the Drosophila melanogaster Metchnik…Available SinceAug. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKM52
Plasmid#154155PurposeFirefly luciferase under the control of the Drosophila melanogaster minimal Hsp70 promoter with 8 copies of the Aedes aegypti Rel2 NF-κB siteDepositorInsertFirefly luciferase
UseLuciferasePromoterDrosophila melanogaster minimal Hsp70 promoter wi…Available SinceAug. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKM51
Plasmid#154154PurposeFirefly luciferase under the control of the Drosophila melanogaster minimal Hsp70 promoter with 8 copies of the Aedes aegypti Rel1A NF-κB siteDepositorInsertFirefly luciferase
UseLuciferasePromoterDrosophila melanogaster minimal Hsp70 promoter wi…Available SinceAug. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDK616
Plasmid#134202PurposeBacterial expression of human Ska1 microtubule binding domain (MTBD) mutantDepositorAvailable SinceFeb. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pIW040
Plasmid#134127PurposeBacterial expression of human Ska1 microtubule binding domain (MTBD)DepositorAvailable SinceFeb. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pIW039
Plasmid#134126PurposeBacterial expression of human Ska1 microtubule binding domain (MTBD)DepositorInsertHis-Precission-human Ska1 MTBD (SKA1 Human)
ExpressionBacterialMutationaa 132-255 and K203A K206AAvailable SinceFeb. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pIW028
Plasmid#134125PurposeBacterial expression of human Ska1 microtubule binding domain (MTBD)DepositorInsertHis-Precission-human Ska1 MTBD (SKA1 Human)
ExpressionBacterialMutationaa 132-255 and K183A K184AAvailable SinceFeb. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-myc-H2-Dd-VN
Plasmid#135517PurposeMammalian expression of VN-fused and myc-tagged H2-DdDepositorInsertH2-Dd (H2-D1 Mouse)
TagsExtracellular c-myc epitope tag, Signal peptide f…ExpressionMammalianMutationC121SPromoterCMVAvailable SinceFeb. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-myc-H2-Ld-VN
Plasmid#135520PurposeMammalian expression of VN-fused and myc-tagged H2-LdDepositorInsertH2-Ld
TagsExtracellular c-myc epitope tag, Signal peptide f…ExpressionMammalianPromoterCMVAvailable SinceFeb. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pIW070
Plasmid#134138PurposeRetroviral expression of GFP-Ska1DepositorInsertGFP-human Ska1 delta MTBD CRISPR/Cas9 hardened (SKA1 Human)
UseRetroviralTagsGFPExpressionMammalianMutationresidues 1-131, CRISPR resistantAvailable SinceJan. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
U6-FnCas12a-TLR-MCV1-gRNA
Plasmid#117412PurposeFnCas12a-gRNA targeting TLR2.0DepositorInsertFnCas12a gRNA targeting TLR 2.0
PromoterU6Available SinceDec. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
DsRed2-M11-92
Plasmid#134467PurposeM11-92 expressionDepositorInsertM1 Spastin Amino Acids 1-92 (SPAST Human)
ExpressionMammalianMutationM1 Spastin Amino Acids 1-92PromoterCMVAvailable SinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
mEmerald-M11-92
Plasmid#134465PurposeM11-92 expressionDepositorInsertM1 Spastin Amino Acids 1-92 (SPAST Human)
ExpressionMammalianMutationM1 Spastin Amino Acids 1-92PromoterCMVAvailable SinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLJM1-zeo-ORF30-2xStrep
Plasmid#130958PurposeLentiviral vector for expression of KSHV ORF30 with a C-terminal 2xStrep tagDepositorInsertORF30 (ORF30 Kaposi's Sarcoma Associated Herpesvirus (HHV-8))
UseLentiviralTags2xStrepPromoterCMVAvailable SinceOct. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLJM1-zeo-ORF24-3xFLAG
Plasmid#130959PurposeLentiviral vector for expression of KSHV ORF24 with a C-terminal 3xFLAG tagDepositorInsertORF24 (ORF24 Kaposi's Sarcoma Associated Herpesvirus (HHV-8))
UseLentiviralTags3xFLAGPromoterCMVAvailable SinceOct. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGWB12_TtNAM-B1
Plasmid#124573PurposeExpresses full-length TtNAM-B1 with an N-terminal FLAG tag under the 35s promoter.DepositorInsertTaNAM-B1
TagsFLAGExpressionPlantPromoter35sAvailable SinceAug. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCR8_TtNAM-B1
Plasmid#124575PurposeTtNAM-B1 CDS in the Gateway-compatible pCR8 vector.DepositorInsertTaNAM-B1
UseTopo gateway cloning vectorPromoterNoneAvailable SinceAug. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCR8_TaNAM-A1(1-217)
Plasmid#124576PurposeNAC domain (1-217) of TaNAM-A1 in the Gateway-compatible pCR8 vector.DepositorInsertTaNAM-A1 (1-217)
UseTopo gateway cloning vectorPromoterNoneAvailable SinceAug. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCR8_TtNAM-B1(1-215)
Plasmid#124577PurposeNAC domain (1-215) of TtNAM-B1 in the Gateway-compatible pCR8 vector.DepositorInsertTtNAM-B1 (1-215)
UseTopo gateway cloning vectorPromoterNoneAvailable SinceAug. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGL4.13/GUG, AAA(30)-FFLuc-3XFLAG
Plasmid#127335PurposeExpress 3xFLAG tagged firefly luciferase (FFLuc) from GUG start codon (AUG at codon 30 mutated to AAA)DepositorInsertFirefly luciferase
Tags3xFLAGExpressionMammalianPromoterSV40Available SinceAug. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
Pmyo-3::hpo-30 gDNA (pBAB204)
Plasmid#117385PurposeAllows for body-wall muscle expression of HPO-30DepositorInsertgenomic DNA of hpo-30 (833 bp)
ExpressionWormAvailable SinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
T7T3-PacI_MafB_ISH
Plasmid#87670PurposeIn situ probe for mouse MafbDepositorAvailable SinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKP105 (pRS416-YBR139Wp-YBR139W-PA-ADH1t)
Plasmid#106461PurposeExpresses Atg42/Ybr139w with a C-terminal PA tag in yeast cellsDepositorInsertYBR139W
TagsPAExpressionBacterial and YeastPromoterYBR139WAvailable SinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKP135 (pRS406-YBR139Wp-YBR139W-PA-ADH1t)
Plasmid#106470PurposeExpresses Atg42/Ybr139w with a C-terminal PA tag in yeast cellsDepositorInsertYBR139W
TagsPAExpressionBacterial and YeastPromoterYBR139WAvailable SinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pX459V2.0-eSpCas9(1.1)+K855A
Plasmid#108298PurposepX459 V2.0 (Plasmid #62988) with the K848A, K855A, K1003A, and R1060A mutationsDepositorTypeEmpty backboneUseCRISPRExpressionMammalianAvailable SinceMay 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pX459V2.0-eSpCas9(1.1)+K810A
Plasmid#108297PurposepX459 V2.0 (Plasmid #62988) with the K810A, K848A, K1003A, and R1060A mutationsDepositorTypeEmpty backboneUseCRISPRExpressionMammalianAvailable SinceMay 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pX459V2.0-eSpCas9(1.1)+K810A+K855A
Plasmid#108299PurposepX459 V2.0 (Plasmid #62988) with the K810A, K848A, K855A, K1003A, and R1060A mutationsDepositorTypeEmpty backboneUseCRISPRExpressionMammalianAvailable SinceMay 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
AY23_pgRNA.R5.2
Plasmid#100295PurposeCCR5-targeting gRNA expression plasmidDepositorInsertgRNA spacer
UseCRISPR and Synthetic BiologyExpressionMammalianPromoterhuman U6 Polymerase III snRNAAvailable SinceSept. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRRG245-Ras-related
Plasmid#99079PurposedsRNA and riboprobe synthesis for Schmidtea mediterranea Ras-related (also known as h.18.4a)DepositorInsertRas-related (also known as h.18.4a) in situ probe
UseT/a cloning vector for dsrna generation and the g…Available SinceAug. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
CDk4-luciferase fragment B
Plasmid#86659PurposeLuciferase reporter containing CDK4 promoterDepositorInsertCDK4 promoter (CDK4 Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutationgenerated by cleavage at the KpnI site from 880 (…PromoterCDK7Available SinceJune 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
GST-RP2-C86Y,P95L
Plasmid#86074PurposeA bacterial expression plasmid encoding ciliary localization effected RP2 (RP2-C86Y,P95L) with N-terminus GST-tag.DepositorInsertRP2 (RP2 Human)
TagsGSTExpressionBacterialMutationChanged cysteine-86 and proline-95 to tyrosine an…PromotertacAvailable SinceMay 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
CFFΔC-EGFP
Plasmid#86059PurposeA mammalian expression plasmid encoding fibrocystin mimentic clone CFFΔC (CD8a luminal domain, fibrocystin transmembrane and ciliary targeting signal) with C-terminus EGFP.DepositorInsertFibrocystin (Pkhd1 Mouse)
TagsGFPExpressionMammalianMutationcontains CD8a luminal and transmembrane domains a…PromoterCMVAvailable SinceMay 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
GST-cystin-CTS
Plasmid#86066PurposeA bacterial expression plasmid encoding cystin ciliary targeting signal with N-terminus GST-tag.DepositorInsertCystin (Cys1 Mouse)
TagsGSTExpressionBacterialMutationcontains the region from 28-35PromotertacAvailable SinceMay 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
CFF-Myc
Plasmid#86062PurposeA mammalian expression plasmid encoding fibrocystin mimentic clone CFF (CD8a luminal domain, fibrocystin transmembrane and cytosolic domains) with C-terminus Myc-tag.DepositorInsertFibrocystin (Pkhd1 Mouse)
TagsMycExpressionMammalianMutationcontains luminal and transmembrane domains of CD8…PromoterCMVAvailable SinceMay 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
CFFΔC-Myc
Plasmid#86060PurposeA mammalian expression plasmid encoding fibrocystin mimentic clone CFFΔC (CD8a luminal domain, fibrocystin transmembrane and ciliary targeting signal domains) with C-terminus Myc-tag.DepositorInsertFibrocystin (Pkhd1 Mouse)
TagsMycExpressionBacterialMutationcontains CD8a luminal and transmembrane domains a…PromoterCMVAvailable SinceMay 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pY036_ATP1A1_G3
Plasmid#86617PurposeExpresses the ATP1A1 G3 crRNA in combination with AsCpf1-3xHA to target ATP1A1 exon 4. U6-crRNA(ATP1A1 G3)-CBh-AsCpf1DepositorInsertATP1A1 G3 crRNA + AsCpf1-3xHA
UseCRISPR; Co-selection via nhej using ouabainTags3xHAExpressionMammalianPromoterCBhAvailable SinceMarch 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDECKO-mCherry MALAT1_Enhancer.4
Plasmid#78539PurposeExperimental plasmidDepositorInsertgRNA1+scaffold+H1promoter+gRNA2
UseLentiviralExpressionMammalianPromoterU6 and U6 (for expressing sgRNA)Available SinceAug. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDECKO-mCherry MALAT1_Enhancer.3
Plasmid#78538PurposeExperimental plasmidDepositorInsertgRNA1+scaffold+H1promoter+gRNA2
UseLentiviralExpressionMammalianPromoterU6 and U6 (for expressing sgRNA)Available SinceJuly 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDECKO-mCherry MALAT1_Exon.3
Plasmid#78542PurposeExperimental plasmidDepositorInsertgRNA1+scaffold+H1promoter+gRNA2
UseLentiviralExpressionMammalianPromoterU6 and U6 (for expressing sgRNA)Available SinceJuly 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDECKO-mCherry MALAT1_Enhancer.2
Plasmid#78537PurposeExperimental plasmidDepositorInsertgRNA1+scaffold+H1promoter+gRNA2
UseLentiviralExpressionMammalianPromoterU6 and U6 (for expressing sgRNA)Available SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pNC2
Plasmid#72438PurposeProduces Escherichia coli N5-carboxyaminoimidazole ribonucleotide mutase (EcPurE1); NCAIR synthetase (EcPurK)DepositorInsertsN5-carboxyaminoimidazole ribonucleotide mutase
N5-carboxyaminoimidazole ribonucleotide synthetase
ExpressionBacterialMutationPro64 to Arg; Asp65 to His; Gln206 to ArgPromoterT7 and n/aAvailable SinceMay 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET30Xa-SBL1(160E)
Plasmid#66846PurposeExpresses soybean lipoxygenase-1 S160E mutant with N-term HistagDepositorTagsHis-tagExpressionBacterialMutationS160EPromoterT7lacAvailable SinceSept. 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET30Xa-SBL1(160S,NoCys)
Plasmid#66877PurposeExpresses a cysteine-free version of WTsoybean lipoxygenase-1 160S (4Cys to Ser) with N-term HistagDepositorInsertsoybean lipoxygenase-1
TagsHistagExpressionBacterialMutationfour (all) cysteines mutated to serinePromoterT7lacAvailable SinceAug. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pNB682
Plasmid#60143PurposeFirefly luciferase (Promega) and yeast codon-optimized yellow fluorescent protein (yEVenus) fusion with PEST degron driven by MET17 promoter.DepositorInsertFLuc-yEVenus-PEST
TagsPEST degronExpressionYeastMutationA4VPromoterMET17prAvailable SinceMarch 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1-hRIP1-FL I541P
Plasmid#61381PurposeExpresses Human full length RIPK1 with I541P mutation in the mammalian cellsDepositorInsertFull length human RIP1with I541P mutation (RIPK1 Human)
TagsEGFP and HA tagExpressionMammalianMutationchanged I541 to PAvailable SinceJan. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pUASp-Flag-Myc-Qin-Tudor-attB
Plasmid#44210DepositorInsertQin-Tudor-domain (qin Fly)
UseFly transformationTagsFlag and MycMutationlacks E3 domain on N-term (del aa1-274)Available SinceOct. 31, 2014AvailabilityAcademic Institutions and Nonprofits only