We narrowed to 5,870 results for: SPI;

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pC0.006

Plasmid

#119670

Purpose

Level 0 part. Promoter, cpc560 promoter, extended spacer region, upstream TFB site region duplicated.

Depositor

Alistair McCormick

Article

Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

Insert

Pcpc560-Ux2

Use

Synthetic Biology

Mutation

bp 89 T to C, bp 187 C to A, Duplicated upstream …

Available Since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA5-puro-eGFP-HEC1-d80

Plasmid

#114025

Purpose

Inducible expression of eGFP-tagged HEC1-d80 in FLPin Trex cell lines

Depositor

Geert Kops

Article

Etemad et al Nat Commun. 2015 Dec 1;6:8987. doi: 10.1

Insert

eGFP-d80-HEC1 (NDC80) (NDC80 Human)

Expression

Mammalian

Mutation

Hec1 - 1 to 80 deleted

Available Since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA5-hygro-eGFP-HEC1-d80

Plasmid

#114030

Purpose

Inducible expression of eGFP-tagged HEC1-d80 in FLPin Trex cell lines

Depositor

Geert Kops

Article

Etemad et al Nat Commun. 2015 Dec 1;6:8987. doi: 10.1

Insert

eGFP-d80-HEC1 (NDC80) (NDC80 Human)

Expression

Mammalian

Mutation

Hec1 - 1 to 80 deleted

Available Since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA5-hygro-eGFP-HEC1-8A/S15D

Plasmid

#114031

Purpose

Inducible expression of eGFP-tagged HEC1-8A/S15D in FLPin Trex cell lines

Depositor

Geert Kops

Article

Etemad et al Nat Commun. 2015 Dec 1;6:8987. doi: 10.1

Insert

eGFP-HEC1-8A/S15D (NDC80) (NDC80 Human)

Expression

Mammalian

Mutation

Hec1 - 8A/S15D

Available Since

Oct. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pmCherry_C1 I3

Plasmid

#61398

Purpose

Expresses mCherry-tagged Inhibitor-3 in mammalian cells

Depositor

Hemmo Meyer

Article

Eiteneuer et al EMBO J. 2014 Oct 8. pii: e201489054.

Insert

Inhibitor-3 (PPP1R11 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 25, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA5FRT/TO-GFP-I3

Plasmid

#61401

Purpose

Expresses GFP-tagged Inhibitor-3 in mammalian cells

Depositor

Hemmo Meyer

Article

Eiteneuer et al EMBO J. 2014 Oct 8. pii: e201489054.

Insert

Inhibitor-3 (PPP1R11 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 25, 2015

Availability

Academic Institutions and Nonprofits only
pT7 cyclin A1-164 venus cks1 (2196)

Plasmid

#61839

Purpose

expression of N terminal cyclin A and cks1

Depositor

Jonathon Pines

Article

Di Fiore et al J Cell Biol. 2010 Aug 23;190(4):501-9.

Insert

cyclin A (CCNA2 Human)

Use

In vitro transcription

Tags

Venus

Mutation

L2V

Promoter

T7

Available Since

June 8, 2015

Availability

Academic Institutions and Nonprofits only
pMSCV-puro-mMCL1 Delta C22

Plasmid

#45820

Depositor

Joseph Opferman

Article

Perciavalle et al Nat Cell Biol. 2012 Apr 29;14(6):575-83.

Insert

mMCL-1 Delta C22

Expression

Mammalian

Mutation

C-terminal 22 amino acids are deleted from full l…

Available Since

July 24, 2013

Availability

Academic Institutions and Nonprofits only
pMSCV-puro-Delta N67-mMCL1

Plasmid

#45819

Depositor

Joseph Opferman

Article

Perciavalle et al Nat Cell Biol. 2012 Apr 29;14(6):575-83.

Insert

Delta N67-mMCL-1

Expression

Mammalian

Mutation

N-terminus 67 amino acids deleted from full lengt…

Available Since

July 24, 2013

Availability

Academic Institutions and Nonprofits only
pBABE-miR-28

Plasmid

#35686

Depositor

George Calin

Article

Almeida et al Gastroenterology. 2012 Jan 10.

Insert

hsa-miR-28 (MIR28 Human)

Use

Retroviral

Expression

Mammalian

Available Since

Sept. 10, 2012

Availability

Academic Institutions and Nonprofits only
PGK-H2BmCherry

Plasmid

#21217

Depositor

Mark Mercola

Article

Kita-Matsuo et al PLoS ONE. 2009 . 4(4):e5046.

Insert

Phosphoglycerate kinase promoter (PGK1 Human)

Use

Lentiviral

Tags

H2B-mCherry

Available Since

July 1, 2009

Availability

Academic Institutions and Nonprofits only

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pLenti-Tet-coBxb1-2A-BFP_IRES-iCasp9-2A-Blast_rtTA3

Plasmid

#171588

Purpose

Lentivector encoding a DNA recombinase landing pad with Bxb1 integrase and iCasp9 negative selection behind a Tet-inducible promoter

Depositor

Kenneth Matreyek

Article

Shukla et al PLoS Pathog. 2021 Jul 16;17(7):e1009715.

Inserts

Bxb1 integrase
iCasp9
BFP
rtTA3G

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available Since

July 21, 2021

Availability

Academic Institutions and Nonprofits only

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pJRH-1363 LV EF1a-CD19 IRES-EGFP

Plasmid

#201919

Purpose

2nd generation lentiviral transfer plasmid for engineering cells to express human CD19

Depositor

Jennifer Doudna

Article

Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

Insert

CD19 (CD19 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-7 asyn WT

Plasmid

#36046

Depositor

Hilal Lashuel

Article

Paleologou et al J Biol Chem. 2008 Jun 13;283(24):16895-9

Insert

alpha-synuclein (SNCA Human)

Expression

Bacterial

Promoter

T7

Available Since

July 13, 2012

Availability

Academic Institutions and Nonprofits only

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AAV9retro

Plasmid

#224687

Purpose

AAV packaging plasmid expressing Rep/Cap genes

Depositor

Zhigang He

Article

Zhang et al Cell. 2024 May 3:S0092-8674(24)00447-1.

Insert

Rep2/Cap9retro

Use

AAV

Mutation

insertion of 10-mer peptide from AAV2retro (LADQD…

Available Since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pJRH-1365 LV EF1a-CD28 IRES-EGFP

Plasmid

#201921

Purpose

2nd generation lentiviral transfer plasmid for engineering cells to express human CD28

Depositor

Jennifer Doudna

Article

Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

Insert

CD28 (CD28 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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tdTomato-KIF1A

Plasmid

#102850

Purpose

Overexpresses KIF1A and cell fill TdTomato

Depositor

Erik Dent

Article

McVicker et al Nat Commun. 2016 Sep 23;7:12741. doi: 10

Insert

KIF1A (Kif1a Rat)

Tags

tdTomato

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 30, 2018

Availability

Academic Institutions and Nonprofits only

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TUBB5-Halo

Plasmid

#64691

Purpose

Expresses beta-tubulin labeled with Halo-tag

Depositor

Yasushi Okada

Article

Uno et al Nat Chem. 2014 Aug;6(8):681-9. doi: 10.1

Insert

TUBB

Tags

Halo-tag

Expression

Mammalian

Available Since

June 12, 2015

Availability

Academic Institutions and Nonprofits only

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pJRH-1345 U6-B2M sgRNA Gag-Cas9 v2

Plasmid

#201916

Purpose

Cas9-EDV production plasmid. Expresses the v2 Gag-Cas9 polypeptide (CAG promoter) and B2M sgRNA (human U6 promoter, 5’-GAGTAGCGCGAGCACAGCTA)

Depositor

Jennifer Doudna

Article

Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

Inserts

Gag-Cas9 v2
B2M sgRNA (Target-specific sequence: 5'- GAGTAGCGCGAGCACAGCTA)

Expression

Mammalian

Promoter

CAG and Human U6

Available Since

Aug. 4, 2023

Availability

Academic Institutions and Nonprofits only

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mEGFP-HRas

Plasmid

#18662

Depositor

Karel Svoboda

Article

Yasuda et al Nat Neurosci. 2006 Feb . 9(2):283-91.

Insert

H-Ras (HRAS Human)

Tags

monomeric EGFP

Expression

Mammalian

Promoter

CMV

Available Since

July 2, 2008

Availability

Academic Institutions and Nonprofits only

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