We narrowed to 11,997 results for: pcDNA

1. pcDNA3.3−3xFLAG−AGO2(D669A)

   Plasmid

   #220461

   Purpose

   Express catalytically-dead mutant human AGO2 with N-terminal 3xFLAG tag in mammalian cells

   Depositor

   David Bartel

   Article

   Wang et al Mol Cell. 2024 Jul 10:S1097-2765(24)0053

   Insert

   Human AGO2 (AGO2 Human)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Aspartate 669 to alanine

   Promoter

   CMV

   Available since

   July 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1+_FH-AGO2-5xE

   Plasmid

   #92008

   Purpose

   Expresses FLAG-HA-AGO2 [S824E, S828E, T830E, S831E, S834E (5xE)]

   Depositor

   Joshua Mendell

   Article

   Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

   Insert

   AGO2 (AGO2 Human)
   

   Use

   Tags

   FLAG-HA

   Expression

   Mammalian

   Mutation

   S824E, S828E, T830E, S831E, S834E (5xE)

   Promoter

   Available since

   Aug. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1/opto-b2AR-EYFP

   Plasmid

   #20948

   Purpose

   Mammalian expression of opto-b2AR fused to EYFP for optical control of GPCR signaling

   Depositor

   Karl Deisseroth

   Article

   Airan et al Nature. 2009 Apr 23;458(7241):1025-9. do

   Insert

   opto-b2AR
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 28, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCDNA5/FRT-ChemoL-CaM

   Plasmid

   #193830

   Purpose

   CMV overexpression of ChemoL-CaM in mammalian cell lines

   Depositor

   Julien Hiblot

   Article

   Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

   Insert

   ChemoL-CaM
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   EGFP:A206K, E299D; HT7: L271E

   Promoter

   CMV

   Available since

   June 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1-TAT-1-101-FLAG

   Plasmid

   #35981

   Depositor

   Susana Valente

   Article

   Mousseau et al Cell Host Microbe. 2012 Jul 19;12(1):97-

   Insert

   transactivating protein (tat )
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   tat from HIV1 101 amino acids

   Promoter

   Available since

   Sept. 6, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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6. Yellow Cameleon-Nano65/pcDNA3

   Plasmid

   #51965

   Purpose

   mammalian expression of Yellow Cameleon-Nano65, an ultrasensitive Ca2+ indicator with Kd = 65 nM

   Depositor

   Takeharu Nagai

   Article

   Horikawa et al Nat Methods. 2010 Sep;7(9):729-32. doi:

   Insert

   Yellow Cameleon-Nano65
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 15, 2014

   Availability

   Academic Institutions and Nonprofits only

7. mScarletI-T2A-mTq2 pcDNA3.1

   Plasmid

   #230048

   Purpose

   Expresses mScarletI-T2A-mTq2 in human cells

   Depositor

   Bas Teusink

   Article

   Botman et al bioRxiv 2024.11.29.626064

   Insert

   mScarletI-T2A-mTq2
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 23, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pcDNAintron-CHIKV-Envelope protein

   Plasmid

   #215700

   Purpose

   Produces the Chikungunya virus envelope protein E3-E2-6K-E1

   Depositor

   Melinda Brindley

   Article

   Reyes Ballista et al J Virol. 2024 Jul 15:e0077524. doi: 10.1

   Insert

   CHIKV E3-E2-6K-E1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 1, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pcDNAintron-CHIKV-capsid protein

   Plasmid

   #215701

   Purpose

   Produces the Chikungunya virus capsid protein

   Depositor

   Melinda Brindley

   Article

   Reyes Ballista et al J Virol. 2024 Jul 15:e0077524. doi: 10.1

   Insert

   CHIKV capsid
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 1, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. pcDNA3. Flag-CSL R192H

    Plasmid

    #167005

    Purpose

    Mammalian expression of CSL R192H

    Depositor

    Gian-Paolo Dotto

    Article

    Bottoni et al Nat Commun. 2019 Aug 29;10(1):3884. doi:

    Insert

    CSL (RBPJ Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    R192H

    Promoter

    CMV

    Available since

    April 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3-HA-CUL1 delta N53

    Plasmid

    #19950

    Depositor

    Yue Xiong

    Article

    Liu et al Mol Cell. 2002 Dec . 10(6):1511-8.

    Insert

    cullin 1 delta N53 (CUL1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    deletion of 53 residues from the NH2-terminus(de…

    Promoter

    Available since

    April 1, 2009

    Availability

    Academic Institutions and Nonprofits only

12. pcDNA3.1 Hygro EYFP H148Q

    Plasmid

    #25873

    Depositor

    Peter Haggie

    Article

    Galietta et al FEBS Lett. 2001 Jun 22. 499(3):220-4.

    Insert

    EYFP H148Q
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    EYFP from pEYFP (Clontech) with H148Q mutation

    Promoter

    Available since

    Aug. 16, 2010

    Availability

    Academic Institutions and Nonprofits only

13. pCDNA3-V5-PTPN14-wild type

    Plasmid

    #61003

    Purpose

    Expresses wild type human PTPN14 in mammalian cells, N-terminus V5 tag

    Depositor

    Jianmin Zhang

    Article

    Wilson et al J Biol Chem. 2014 Aug 22;289(34):23693-7

    Insert

    PTPN14 (PTPN14 Human)
    

    Use

    Tags

    V5 tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3-HA-CUL1 delta C22

    Plasmid

    #19942

    Depositor

    Yue Xiong

    Article

    Furukawa et al Mol Cell Biol. 2000 Nov . 20(21):8185-97

    Insert

    cullin 1 delta C22 (CUL1 Human)
    

    Use

    Tags

    HA2

    Expression

    Mammalian

    Mutation

    Mutation of 22 amino acids from the C terminus

    Promoter

    Available since

    April 17, 2009

    Availability

    Academic Institutions and Nonprofits only

15. 1432 pcDNA3 flag MAGI1c

    Plasmid

    #10714

    Depositor

    William Sellers

    Article

    Vazquez et al J Biol Chem. 2001 Dec 28. 276(52):48627-

    Insert

    MAGI-1 (Magi1 Mouse)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 28, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3-CaMPARI W391F V398L

    Plasmid

    #60787

    Purpose

    Expresses CaMPARI W391F V398L in mammalian cells, a photoconvertible fluorescent protein-based calcium integrator

    Depositor

    Eric Schreiter, Loren Looger

    Article

    Fosque et al Science. 2015 Feb 13;347(6223):755-60. d

    Insert

    CaMPARI W391F V398L
    

    Use

    Tags

    Nuclear Export Signal

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 26, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3 MDM2 S166D S186D

    Plasmid

    #16236

    Depositor

    Mien-Chie Hung

    Article

    Zhou et al Nat Cell Biol. 2001 Nov . 3(11):973-82.

    Insert

    MDM2 (MDM2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Ser166 and Ser186 replaced by Asp

    Promoter

    Available since

    Nov. 30, 2007

    Availability

    Academic Institutions and Nonprofits only

18. pcDNA5-FRT-GFP-MYC

    Plasmid

    #185375

    Purpose

    For mammalian expression of human GFP-MYC

    Depositor

    Ayaz Najafov

    Insert

    MYC (MYC Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    June 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. 809 pcDNA3 GFP PTEN

    Plasmid

    #10759

    Depositor

    William Sellers

    Article

    Vazquez et al J Biol Chem. 2001 Dec 28. 276(52):48627-

    Insert

    PTEN (PTEN Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 13, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA-flag-mKdm4d-polyA

    Plasmid

    #61553

    Purpose

    For mRNA synthesis or expression in mammalian cells

    Depositor

    Yi Zhang

    Article

    Matoba et al Cell. 2014 Nov 6;159(4):884-95. doi: 10.

    Insert

    Kdm4d (Kdm4d Mouse)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and T7

    Available since

    Feb. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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