We narrowed to 54,624 results for: tra

1. pLX304-EZH1-D745N

   Plasmid

   #203585

   Purpose

   Expresses V5-tagged mutant version of EZH1 partially resistant to JQ-EZ-05 in mammalian cells.

   Depositor

   Abhishek Chakraborty, William Kaelin

   Article

   Chakraborty et al Sci Transl Med. 2017 Jul 12;9(398):eaal5

   Insert

   EZH1 (EZH1 Human)
   

   Use

   Lentiviral

   Tags

   V5-tagged

   Expression

   Mammalian

   Mutation

   changed Aspartic Acid 745 to Asparagine for parti…

   Promoter

   CMV

   Available since

   Feb. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pOTTC1822 - pAAV SYN1 DIO hChR2(H134R)-V5-ER-LBD (ChRERa)

   Plasmid

   #201821

   Purpose

   An adeno-associated viral vector expressing Cre-dependent channelrhodopsin-2 fused to a V5 epitope and the ligand-binding domain of estrogen receptor (ChRERa) from a synapsin promoter, for use in PET

   Depositor

   Christopher Richie

   Article

   Bonaventura et al Sci Transl Med. 2023 Jul 26;15(706):eadd

   Insert

   hChR2(H134R)-V5-ER-LBD (ChRERa)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   SYN1

   Available since

   Oct. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pOTTC1845 - pAAV CaMKII hChR2(H134R)-V5-ER-LBD (ChRERa)

   Plasmid

   #201822

   Purpose

   An adeno-associated viral vector expressing channelrhodopsin-2 fused to a V5 epitope and the ligand-binding domain of estrogen receptor (ChRERa) from a CaMKii promoter, for use in PET imaging

   Depositor

   Christopher Richie

   Article

   Bonaventura et al Sci Transl Med. 2023 Jul 26;15(706):eadd

   Insert

   hChR2(H134R)-V5-ER-LBD (ChRERa)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   CaMKii

   Available since

   Oct. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP (AAV Retrograde)

   Viral Prep

   #112677-AAVrg

   Purpose

   Ready-to-use AAV Retrograde particles produced from pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP (#112677). In addition to the viral particles, you will also receive purified pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP plasmid DNA. EF1a-driven, Cre-dependent color switch. This AAV directs expression of nuclear mCherry in the absence of Cre. In the presence of Cre, nuclear EGFP (and not mCherry) will be expressed. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Brandon Harvey

   Article

   Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

   Promoter

   EF1a

   Tags

   mCherry (Cre-negative cells), EGFP (Cre-positive cells)

   Available since

   July 16, 2019

   Availability

   Academic Institutions and Nonprofits only

5. pAAV-S5E2-dTom-nlsdTom (AAV PHP.eB)

   Viral Prep

   #135630-PHPeB

   Purpose

   Ready-to-use AAV PHP.eB particles produced from pAAV-S5E2-dTom-nlsdTom (#135630). In addition to the viral particles, you will also receive purified pAAV-S5E2-dTom-nlsdTom plasmid DNA. Bicistronic expression of dTomato and nuclear-targeted dTomato under the control of the E2 regulatory element. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Jordane Dimidschstein

   Article

   Vormstein-Schneider et al Nat Neurosci. 2020 Dec;23(12):1629-1636.

   Promoter

   Tags

   dTomato

   Available since

   Oct. 28, 2021

   Availability

   Academic Institutions and Nonprofits only

6. pAAV-S5E2-ChR2-mCherry (AAV PHP.eB)

   Viral Prep

   #135634-PHPeB

   Purpose

   Ready-to-use AAV PHP.eB particles produced from pAAV-S5E2-ChR2-mCherry (#135634). In addition to the viral particles, you will also receive purified pAAV-S5E2-ChR2-mCherry plasmid DNA. Expression of ChR2-mCherry under the control of the E2 regulatory element. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Jordane Dimidschstein

   Article

   Vormstein-Schneider et al Nat Neurosci. 2020 Dec;23(12):1629-1636.

   Promoter

   Tags

   mCherry

   Available since

   Oct. 28, 2021

   Availability

   Academic Institutions and Nonprofits only

7. pAAV-S5E2-GFP-fGFP (AAV PHP.eB)

   Viral Prep

   #135631-PHPeB

   Purpose

   Ready-to-use AAV PHP.eB particles produced from pAAV-S5E2-GFP-fGFP (#135631). In addition to the viral particles, you will also receive purified pAAV-S5E2-GFP-fGFP plasmid DNA. Bicistronic expression of GFP and membrane-targeted GFP under the control of the E2 regulatory element. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Jordane Dimidschstein

   Article

   Vormstein-Schneider et al Nat Neurosci. 2020 Dec;23(12):1629-1636.

   Promoter

   Tags

   EGFP

   Available since

   Nov. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

8. pAAV-S5E2-Gq-P2A-dTomato (AAV PHP.eB)

   Viral Prep

   #135635-PHPeB

   Purpose

   Ready-to-use AAV PHP.eB particles produced from pAAV-S5E2-Gq-P2A-dTomato (#135635). In addition to the viral particles, you will also receive purified pAAV-S5E2-Gq-P2A-dTomato plasmid DNA. Expression of Gq-DREADD and dTomato (physically separate) under the control of the E2 regulatory element. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Jordane Dimidschstein

   Article

   Vormstein-Schneider et al Nat Neurosci. 2020 Dec;23(12):1629-1636.

   Promoter

   Tags

   dTomato (physically separate, not a fusion protein)

   Available since

   Aug. 27, 2021

   Availability

   Academic Institutions and Nonprofits only

9. pAAV-S5E2-C1V1-eYFP (AAV PHP.eB)

   Viral Prep

   #135633-PHPeB

   Purpose

   Ready-to-use AAV PHP.eB particles produced from pAAV-S5E2-C1V1-eYFP (#135633). In addition to the viral particles, you will also receive purified pAAV-S5E2-C1V1-eYFP plasmid DNA. Expression of red-shifted opsin C1V1 fused to EYFP under the control of the E2 regulatory element. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Jordane Dimidschstein

   Article

   Vormstein-Schneider et al Nat Neurosci. 2020 Dec;23(12):1629-1636.

   Promoter

   Tags

   EYFP

   Available since

   Aug. 27, 2021

   Availability

   Academic Institutions and Nonprofits only

10. pDGB1alpha2_KanR_BastaR_Rb7_GFP

    Plasmid

    #186427

    Purpose

    optimisation of phosphinothricin (Basta) selection in plant cells; combines kanamycin resistance gene (nptII) and Basta resistance gene (bar) with fluorescent marker (eGFP)

    Depositor

    Lukas Fischer

    Article

    Kobercova et al Plant Cell Rep. 2023 Jan 7. doi: 10.1007

    Inserts

    KanR
    BastaR
    Rb7
    eGFP
    

    Use

    Synthetic Biology; Binary vector for escherichia …

    Tags

    Expression

    Plant

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    CaMV 35S and Pnos

    Available since

    Jan. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. MLRV2: NF-kb-SMAD-DBE-P53-AP1

    Plasmid

    #124536

    Purpose

    Multiplex luciferase reporter vector, with luciferase reporters for the pathways NFKB, SMAD, FOXO, P53 and AP1

    Depositor

    Koen Venken

    Article

    Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e1

    Inserts

    5 copies of the NF-kb DNA binding motif
    7xSMAD_RE::FLuc
    3xDBE_BE::Renilla (FOXO)
    2xP53_RE::NLuc
    6xAP-1_RE::GrRenilla
    

    Use

    Luciferase and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. MLRV3:HRE-P53-MAPK/JNK-FOXO-TGFβ

    Plasmid

    #178320

    Purpose

    Multiplex luciferase reporter vector, with luciferase reporters for the pathways HRE, P53, AP-1, FOXO and SMAD.

    Depositor

    Koen Venken

    Article

    Sarrion-Perdigones et al Methods Mol Biol. 2022;2524:409-432. doi

    Inserts

    HRE RedFirefly reporter
    P53 FLuc reporter
    AP-1 Renilla reporter
    FOXO NLuc Reporter
    SMAD GrRenilla reporter
    

    Use

    Luciferase and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTwist-SARS-CoV-2 Δ18 B.1.617.2v1

    Plasmid

    #179905

    Purpose

    Encodes SARS-CoV-2 B.1.1.529 Spike Protein lacking 18 C-terminal amino acids for pseudovirus production

    Depositor

    Alejandro Balazs

    Article

    Garcia-Beltran et al Cell. 2022 Jan 6. pii: S0092-8674(21)014

    Insert

    SARS-CoV-2 B.1.617.2v1 Spike truncated to remove 18 amino acids (S Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    T19R; G142D; E156G; Δ157-158; L452R; T478K; D614G…

    Promoter

    CAG

    Available since

    Jan. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAGGS-PDGFRb-IRES2-EGFP

    Plasmid

    #204354

    Purpose

    Expresses wild-type PDGFRb gene.

    Depositor

    Hirofumi Nakatomi

    Article

    Shima et al Sci Transl Med. 2023 Jun 14;15(700):eabq

    Insert

    Platelet derived growth factor receptor beta (PDGFRB) (PDGFRB Human)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRS2 (CK2alpha', CK2beta)

    Plasmid

    #27093

    Depositor

    David Litchfield

    Article

    Turowec et al Methods Enzymol. 2010;484:471-93.

    Inserts

    CK2beta (CSNK2B Human)
    CK2alpha' (CSNK2A2 Human)
    

    Use

    Tetracycline-regulated expression

    Tags

    HA and Myc

    Expression

    Mammalian

    Mutation

    Promoter

    bidirectional tet-responsive promoter

    Available since

    Oct. 14, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGV13 (CK2alpha K68M, CK2beta)

    Plasmid

    #27094

    Depositor

    David Litchfield

    Article

    Turowec et al Methods Enzymol. 2010;484:471-93.

    Inserts

    CK2beta (CSNK2B Human)
    CK2alpha K68M (CSNK2A1 Human)
    

    Use

    Tetracycline-regulated expression

    Tags

    HA on CK2alpha and Myc on CK2beta

    Expression

    Mammalian

    Mutation

    K68M on CK2alpha

    Promoter

    bidirectional tet-responsive promoter and bidirec…

    Available since

    Oct. 14, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGV7 (CK2alpha' K69M, CK2beta)

    Plasmid

    #27095

    Depositor

    David Litchfield

    Article

    Turowec et al Methods Enzymol. 2010;484:471-93.

    Inserts

    CK2beta (CSNK2B Human)
    CK2alpha' K69M (CSNK2A2 Human)
    

    Use

    Tetracycline-regulated expression

    Tags

    HA tag on CK2alpha' and Myc on CK2beta

    Expression

    Mammalian

    Mutation

    K69M on CK2alpha'

    Promoter

    bidirectional tet-responsive promoter

    Available since

    Oct. 14, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDCAF3-IS5

    Plasmid

    #65220

    Purpose

    Expresses N-demethylases for the degredation of caffeine and related methylxanthines. Can be used to measure caffeine content as described in the publication listed below.

    Depositor

    Jeffrey Barrick

    Article

    Quandt et al ACS Synth Biol. 2013 Jun 21;2(6):301-7.

    Inserts

    ndmA
    ndmB
    ndmC
    ndmD
    gst9
    chlR
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Changed start codon from gtg to atg and Changed s…

    Promoter

    BBa_J23100

    Available since

    Jan. 8, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pCEP4-myc-PDGFRA-R914W_EGFP-PEST_reporter

    Plasmid

    #172596

    Purpose

    Mammalian fluorescent (EGFP-PEST) reporter plasmid for PDGFRA-R914W mutant signaling.

    Depositor

    Daniel Kiss

    Article

    Yu et al Hum Mol Genet. 2022 Jul 21;31(14):2348-2

    Insert

    myc-PDGFRA-R914W (PDGFRA Human, Synthetic)
    

    Use

    Egfp-pest_reporter

    Tags

    Extracellular c-myc epitope tag and Signal peptid…

    Expression

    Mammalian

    Mutation

    PDGFRA-R914W

    Promoter

    CMV

    Available since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLViN-iRFP670-α-cateninA+ΔβH

    Plasmid

    #229707

    Purpose

    Lentiviral expression of iRFP670-alpha-cateninA+delta-beta-H in mammalian cells

    Depositor

    Alpha Yap

    Article

    Noordstra et al Dev Cell. 2023 Sep 25;58(18):1748-1763.e

    Insert

    CTNNA1 (alpha-catenin) Human (CTNNA1 Human)
    

    Use

    Lentiviral

    Tags

    iRFP670

    Expression

    Mammalian

    Mutation

    amino acids 670-673, RAIM--> GSGS

    Promoter

    CMV promoter

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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