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Showing: 6421 - 6450 of 8432 results

  3. pTEI128

    Plasmid
    #126209
    Purpose
    Module 1 vector of PlaMiNGo toolkit for cloning of candidate gene at N-terminus of GFP11x3 and expression in plant cell
    Depositor
    Ralf-Bernd Klösgen
    Article
    Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs
    Inserts
    ccdB Cassette (BsaI)
    GFP11x3 (5 aa linker)
    Use
    Binary, module 1
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    126209-plasmid-map-sequence-id-246907

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  7. pEPOR2KN0039

    Plasmid
    #117606
    Purpose
    Level2 MoClo construct, containing level1 plant expression cassettes for AsCas12a(pEPOR1CB0014) and sgRNA_AtADH1 {AT1G77120}(pEPOR1CB0028)
    Depositor
    Nicola Patron
    Article
    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do
    Insert
    [35S:AsCas12a(pEPOR1CB0014) ] +[AtU6-26:sgRNA]
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Nov. 16, 2018
    Availability
    Academic Institutions and Nonprofits only
    117606-plasmid-map-sequence-id-228336

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  13. pEPOR2KN0079

    Plasmid
    #117646
    Purpose
    Level2 MoClo construct, containing level1 plant expression cassettes for SaCas9(pEPOR1CB0015) and sgRNA_ENTH/ANT {AT1G14910}(pEPOR1CB0100)and sgRNA_ENTH/ANT {AT2G01600}(pEPOR1CB0111)
    Depositor
    Nicola Patron
    Article
    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do
    Insert
    [35S:SaCas9(pEPOR1CB0015) ] +[AtU6-26:sgRNA]
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Nov. 16, 2018
    Availability
    Academic Institutions and Nonprofits only
    117646-plasmid-map-sequence-id-228398

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  15. pCALNL-eGFP-Esp3I

    Plasmid
    #81226
    Purpose
    Cloning plasmid derived from loxP reporter. The Kan and pA where digested and removed with XhoI/MluI and replaced with two Esp3I sites
    Depositor
    David Liu
    Article
    Chaikind et al Nucleic Acids Res. 2016 Aug 11. pii: gkw
    Insert
    Esp31-Neo-Esp31 deletion cassette upstream of EGFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    pCBa
    Available since
    March 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    81226-plasmid-map-sequence-id-168098

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  16. pCALNL_Ch10

    Plasmid
    #81224
    Purpose
    pCALNL reporter contains two target sites consisting of PAM Cas9 site-gix psuedo site-Cas9 site-PAM that match region in PCDH15 locus on chromosome 10
    Depositor
    David Liu
    Article
    Chaikind et al Nucleic Acids Res. 2016 Aug 11. pii: gkw
    Insert
    gix-Neo-gix deletion cassette upstream of EGFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    pCBa
    Available since
    Nov. 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    81224-plasmid-map-sequence-id-163294

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  17. pGS1044

    Plasmid
    #63797
    Purpose
    Vector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal 3xFLAG tag and a LEU2 selection marker
    Depositor
    Bjoern Sander, Monika Golas
    Article
    Lin et al Biochim Biophys Acta. 2015 Mar;1854(3):1
    Insert
    3xFLAG followed by KlLEU2
    Use
    E. coli cloning vector
    Tags
    3xFLAG
    Expression
    Mutation
    Promoter
    none
    Available since
    April 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    63797-plasmid-map-sequence-id-110117

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  18. NBS1-Halo HRD

    Plasmid
    #207084
    Purpose
    Homologous recombination donor for insertion of a HaloTag at the C-terminus of the endogenous NBS1 locus.
    Depositor
    Jens Schmidt
    Article
    Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75
    Insert
    HaloTag followed by a LoxP-PuroR cassette flanked by human NBS1 locus sequences
    Use
    Tags
    HaloTag
    Expression
    Mammalian
    Mutation
    Promoter
    None
    Available since
    May 15, 2025
    Availability
    Academic Institutions and Nonprofits only
    207084-plasmid-map-sequence-id-471046

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  19. Halo-ATG16L1 HRD

    Plasmid
    #207537
    Purpose
    Homologous recombination donor to integrate a 3xFLAG-HaloTag at the endogenous ATG16L1 locus in human cells
    Depositor
    Jens Schmidt
    Article
    Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007
    Insert
    HaloTag with internal PuroR cassette flanked by human ATG16L1 locus sequences
    Use
    Tags
    HaloTag
    Expression
    Mammalian
    Mutation
    Promoter
    Endogenous
    Available since
    May 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    207537-plasmid-map-sequence-id-470412

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  20. Rev7-Halo HRD

    Plasmid
    #207079
    Purpose
    Homologous recombination donor for insertion of a HaloTag at the C-terminus of the endogenous REV7 locus.
    Depositor
    Jens Schmidt
    Article
    Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75
    Insert
    HaloTag followed by a PolyA signal and PuroR cassette flanked by human REV7 locus sequences
    Use
    Tags
    HaloTag
    Expression
    Mammalian
    Mutation
    Promoter
    None
    Available since
    May 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    207079-plasmid-map-sequence-id-471050

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  25. pCMK83

    Plasmid
    #226793
    Purpose
    Integrates slr0214 (HIP1 methyltransferase) into gidB-atpI locus of E. coli DH10B
    Depositor
    David Nielsen
    Article
    Kamoku et al Biotechnol Prog. 2025 Jan 23:e3518. doi:
    Insert
    slr0214 (slr0214 Synechocystis sp. PCC 6803)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Tetracycline resistance cassette promoter (consti…
    Available since
    Jan. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    226793-plasmid-map-sequence-id-455428

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  26. EKAREN5-gl

    Plasmid
    #225958
    Purpose
    The YPet and mTurquoise sequence cassettes in EKAREN5(Addgene 167821) were replaced with a synonymous codon variant of YPet and mTurquoise-gl and subcloned into pCSII lentiviral backbone.
    Depositor
    Toru Hiratsuka
    Article
    Weisheng et al Cell Struct Funct. 2024 Dec 18. doi: 10.
    Insert
    EKAREN5-gl
    Use
    Lentiviral
    Tags
    nls localization motif
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1-alpha promoter
    Available since
    Jan. 22, 2025
    Availability
    Academic Institutions and Nonprofits only
    225958-plasmid-map-sequence-id-452543

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  27. Halo-MDC1 HRD

    Plasmid
    #207086
    Purpose
    Homologous recombination donor for insertion of a HaloTag at the N-terminus of the endogenous MDC1 locus.
    Depositor
    Jens Schmidt
    Article
    Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75
    Insert
    HaloTag with internal PuroR cassette flanked by human MDC1 locus sequences
    Use
    Tags
    HaloTag
    Expression
    Mammalian
    Mutation
    Promoter
    Endogenous
    Available since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    207086-plasmid-map-sequence-id-431126

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  28. Halo-ATM HRD

    Plasmid
    #207085
    Purpose
    Homologous recombination donor for insertion of a HaloTag at the N-terminus of the endogenous ATM locus.
    Depositor
    Jens Schmidt
    Article
    Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75
    Insert
    HaloTag with internal PuroR cassette flanked by human ATM locus sequences
    Use
    Tags
    HaloTag
    Expression
    Mammalian
    Mutation
    Promoter
    None
    Available since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    207085-plasmid-map-sequence-id-431785

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  29. ATM-Halo HRD

    Plasmid
    #207082
    Purpose
    Homologous recombination donor for insertion of a HaloTag at the C-terminus of the endogenous ATM locus.
    Depositor
    Jens Schmidt
    Article
    Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75
    Insert
    HaloTag followed by a PolyA signal and PuroR cassette flanked by human ATM locus sequences
    Use
    Tags
    HaloTag
    Expression
    Mammalian
    Mutation
    Promoter
    None
    Available since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    207082-plasmid-map-sequence-id-430992

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  30. Halo-53BP1 HRD

    Plasmid
    #207087
    Purpose
    Homologous recombination donor for insertion of a HaloTag at the N-terminus of the endogenous 53BP1 locus.
    Depositor
    Jens Schmidt
    Article
    Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75
    Insert
    HaloTag with internal PuroR cassette flanked by human 53BP1 locus sequences
    Use
    Tags
    HaloTag
    Expression
    Mammalian
    Mutation
    Promoter
    Endogenous
    Available since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    207087-plasmid-map-sequence-id-431238

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Showing: 6421 - 6450 of 8432 results