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169,450 results

Showing: 10701 - 10750 of 169450 results

  1. DD-DamV133A-tdTomato-LMNB1

    Plasmid
    #159604
    Purpose
    Shield-1 inducible transient mammalian expression of Dam-tdTomato-LMNB1 for DamID, containing the V133A mutant of Dam and a fused tdTomato to monitor fusion protein localization and expression levels.
    Depositor
    Aaron Streets
    Article
    Altemose et al Cell Syst. 2020 Oct 21;11(4):354-366.e9.
    Insert
    DD-DamV133A-tdTomato-LMNB1
    Expression
    Mammalian
    Mutation
    Dam V133A mutation
    Promoter
    CMV
    Available Since
    Sept. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    159604-plasmid-map-sequence-id-311954

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  8. PB CAG-mTagBFP2-T2A-iCreERT2

    Plasmid
    #177308
    Purpose
    CAG-mTagBFP2-T2A-iCre-ERT2 piggyBac transposon
    Depositor
    Mario Capecchi
    Insert
    mTagBFP2-T2A-iCreERT2
    Use
    Cre/Lox; Piggybac
    Expression
    Mammalian
    Available Since
    Nov. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    177308-plasmid-map-sequence-id-348855

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  12. pSBtet-GH

    Plasmid
    #60498
    Purpose
    SB-transposon with inducible SfiI cloning site for GOI (contains firefly luciferase) and constitutive expression of GFP, rtTA and hygromycin resistance gene
    Depositor
    Eric Kowarz
    Article
    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b
    Type
    Empty backbone
    Use
    Transposon
    Expression
    Mammalian
    Promoter
    TCE & RPBSA
    Available Since
    Feb. 11, 2015
    Availability
    Academic Institutions and Nonprofits only
    60498-plasmid-map-sequence-id-97549

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  13. TFORF1324

    Plasmid
    #141810
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    KLF17 (KLF17 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    141810-plasmid-map-sequence-id-427309

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  14. TFORF2168

    Plasmid
    #141954
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    GRHL3 (GRHL3 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    April 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    141954-plasmid-map-sequence-id-391432

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  18. sfCherry11-WT-PTPN22

    Plasmid
    #195384
    Purpose
    Mammalian expression of human wild-type PTPN22 with sfCherry(11) tag
    Depositor
    Patrick Concannon
    Insert
    PTPN22 (PTPN22 Human)
    Tags
    sfCherry(11)
    Expression
    Mammalian
    Available Since
    March 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    195384-plasmid-map-sequence-id-386968

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  20. TFORF1462

    Plasmid
    #143119
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    LMX1B (LMX1B Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    April 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    143119-plasmid-map-sequence-id-391498

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  23. Murine CD3 WTdelta-F2A-gamma-T2A-epsilon-P2A-zeta pMIG II

    Plasmid
    #52092
    Purpose
    Retroviral expression of all four WT CD3 subunits, co-expressed with EGFP
    Depositor
    Dario Vignali
    Article
    Holst et al Nat Immunol. 2008 Jun;9(6):658-66. doi:
    Inserts
    CD3 delta (Cd3d Mouse)
    CDC gamma (Cd3g Mouse)
    CD3 epsilon (Cd3e Mouse)
    CD3 zeta (Cd247 Mouse)
    Use
    Retroviral
    Tags
    F2A, IRES-EGFP, P2A, and T2A
    Expression
    Mammalian
    Available Since
    April 9, 2014
    Availability
    Academic Institutions and Nonprofits only
    52092-plasmid-map-sequence-id-80506

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  25. TFORF0519

    Plasmid
    #141629
    Purpose
    Lentiviral vector for overexpressing the PAX5 transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    PAX5 (PAX5 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    141629-plasmid-map-sequence-id-391535

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  30. pCBh_3x Flag-NLS-OsCas12f1-NLS_pA_phU6_gRNA scaffold-spacer_pCMV_mCherry_pA

    Plasmid
    #197024
    Purpose
    Vector encoding a human codon-optimized OsCas12f1 driven by CBh promoter, guide RNAs compatible with OsCas12f1 driven by hU6, and mCherry driven by CMV promoter
    Depositor
    Xiangfeng Kong
    Article
    Kong et al Nat Commun. 2023 Apr 11;14(1):2046. doi:
    Insert
    humanized OsCas12f1
    Expression
    Mammalian
    Promoter
    CBh, CMV, hU6
    Available Since
    May 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    197024-plasmid-map-sequence-id-388957

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  31. lox2272 Neo* lox2272

    Plasmid
    #168769
    Purpose
    lox2272 (lox variant)-flanked pPgk-pEM7-Neo*-bpA positive selection cassette
    Depositor
    Mario Capecchi
    Insert
    Neo*
    Use
    Cre/Lox and Mouse Targeting
    Expression
    Bacterial and Mammalian
    Available Since
    July 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    168769-plasmid-map-sequence-id-337468

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  35. hGli3 flag3x

    Plasmid
    #84921
    Purpose
    mammalian expression of Gli3
    Depositor
    Martin Fernandez-Zapico
    Insert
    Gli3 (GLI3 Human)
    Tags
    3xFlag
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Nov. 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    84921-plasmid-map-sequence-id-421628

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  36. pUBC_stdMCP_serinemod_E488QADAR_p2A_yGFP

    Plasmid
    #154787
    Purpose
    Expresses synonymized MS2 coat protein dimer (MCP) fused to hyperactive ADAR catalytic domain
    Depositor
    Robert Singer
    Article
    Biswas et al iScience. 2020 Jun 28;23(7):101318. doi:
    Insert
    Adenosine deaminse (Adar Synthetic, Fly)
    Use
    Lentiviral
    Tags
    GFP, V5, and stdMCP
    Expression
    Mammalian
    Mutation
    Synonymized serine to prevent autoediting of ADAR…
    Promoter
    UBC
    Available Since
    July 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    154787-plasmid-map-sequence-id-306329

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  37. pMito-TrxRFP1

    Plasmid
    #98997
    Purpose
    mammalian expression of a biosensor to monitor thioredoxin redox dynamics, mitochondrially locaclized
    Depositor
    Huiwang Ai
    Article
    Fan et al Nat Chem Biol. 2017 Jun 26. doi: 10.1038
    Insert
    TrxRFP1 and mitochondrial localization sequence
    Tags
    His-tag and Mitochondria localization sequence
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    July 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    98997-plasmid-map-sequence-id-189692

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  38. pAAV-syn-FLP-FRT-iGluSnFR4s-NGR-WPRE

    Plasmid
    #234453
    Purpose
    AAV-mediated expression of glutamate sensor with improved sensitivity and slow deactivation kinetics (4s = slow); NGR vector matches or outperforms PDGFR in all conditions tested.
    Depositor
    GENIE Project
    Article
    Aggarwal et al bioRxiv 2025.03.20.643984
    Insert
    iGluSnFR4s-NGR
    Use
    AAV; Flp/frt
    Tags
    IgK chain and Myc epi tag-NGR GPI anchor
    Expression
    Mammalian
    Promoter
    Synapsin
    Available Since
    March 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    234453-plasmid-map-sequence-id-465748

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  42. pFEndoNA2

    Plasmid
    #174667
    Purpose
    Expresses GFP-endoNA2 fusion protein for detection of polysialic acid. The endoNA2 is a catalytically inactive form of endosialidase from bacteriophage PK1A2.
    Depositor
    Jukka Finne
    Article
    Jokilammi et al J Immunol Methods. 2004 Dec;295(1-2):149
    Insert
    GFP-endoNA2 fusion protein
    Tags
    Histag
    Expression
    Bacterial
    Promoter
    T5
    Available Since
    Sept. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    174667-plasmid-map-sequence-id-343065

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  46. pAAV-CAG-SomaGCaMP6f2 (AAV8)

    Viral Prep
    #158757-AAV8
    Purpose
    Ready-to-use AAV8 particles produced from pAAV-CAG-SomaGCaMP6f2 (#158757). In addition to the viral particles, you will also receive purified pAAV-CAG-SomaGCaMP6f2 plasmid DNA. CAG-driven expression of soma-targeting GCaMP6f2. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Edward Boyden
    Article
    Shemesh et al Neuron. 2020 Jun 26. pii: S0896-6273(20)
    Promoter
    CAG promoter
    Available Since
    Feb. 23, 2021
    Availability
    Academic Institutions and Nonprofits only

  47. pcDNA3-HCMVgH-8his (TB40/E)

    Plasmid
    #136445
    Purpose
    Mammalian expression plasmid for HCMV glycoprotein H (TB40/E strain) extracellular domain with 8his purification tag
    Depositor
    Erik Procko
    Article
    Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.
    Insert
    glycoprotein H (UL75 Human betaherpesvirus 5)
    Tags
    Short GGSG linker and 8his tag
    Expression
    Mammalian
    Mutation
    Extracellular domain (a.a. 1-717). Codon-optimize…
    Promoter
    CMV
    Available Since
    July 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    136445-plasmid-map-sequence-id-269879

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Showing: 10701 - 10750 of 169450 results