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173,176 results

Showing: 6451 - 6480 of 173176 results

  5. DT044A

    Plasmid
    #159405
    Purpose
    Inducible Perfringolysin O (PFO) expression and purification, controlled under hybrid IPTG-regulated T7/LacO promoter (pRT30)
    Depositor
    Sheref Mansy
    Article
    Toparlak et al Sci Adv. 2020 Sep 18;6(38). pii: 6/38/ea
    Insert
    pr.T7-LacO-6xHis-PFO (Pfo, Clostridium perfringens)
    Use
    Synthetic Biology
    Tags
    6xHis
    Expression
    Bacterial
    Promoter
    T7/LacO promoter
    Available Since
    Feb. 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    159405-plasmid-map-sequence-id-324289

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  13. TRIPZ-bioGEF-UBnc-PURO

    Plasmid
    #208044
    Purpose
    Enables inducible expression of low-affinity Avitag-UBnc; to perform bioE3; nc = non-cleavable mutation; selection with puromycin
    Depositor
    Rosa Barrio, James Sutherland
    Article
    Barroso-Gomila et al Nat Commun. 2023 Nov 23;14(1):7656. doi:
    Insert
    UBnc (UBC Human)
    Use
    Lentiviral
    Tags
    Low-affinity Avitag
    Mutation
    L73P Mutation near C-terminus suppresses cleavage…
    Promoter
    tetO/UBC
    Available Since
    Nov. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    208044-plasmid-map-sequence-id-410266

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  16. pGP-AAV-syn-FLEX-jGCaMP7s-WPRE (AAV PHP.eB)

    Viral Prep
    #104491-PHPeB
    Purpose
    Ready-to-use AAV PHP.eB particles produced from pGP-AAV-syn-FLEX-jGCaMP7s-WPRE (#104491). In addition to the viral particles, you will also receive purified pGP-AAV-syn-FLEX-jGCaMP7s-WPRE plasmid DNA. Synapsin-driven, Cre-dependent, GCaMP7s calcium sensor. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Douglas Kim, GENIE Project
    Article
    Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi
    Promoter
    Syn
    Available Since
    Aug. 7, 2018
    Availability
    Academic Institutions and Nonprofits only

  17. pCRISPomyces-2BD

    Plasmid
    #234428
    Purpose
    Plasmid for Streptomyces containing gene of modified Cas9-BD protein and sequence of sgRNA cloning template
    Depositor
    Min-Kyu Oh
    Article
    Kim et al Nat Commun. 2025 Jan 21;16(1):874. doi:
    Insert
    Modified Cas9 with polyaspartate linking
    Expression
    Bacterial
    Mutation
    Linked polyaspartate using glycine-serine linker
    Available Since
    April 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    234428-plasmid-map-sequence-id-468848

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  18. dpYPQ230-D156R-ABE8

    Plasmid
    #195569
    Purpose
    dLbCas12a-D156R based adenine base editor, ecTadA8e fused to the N terminal of dLbCas12a-D156R with the linker of (GSAGSAAGSGEF)x3
    Depositor
    Yiping Qi
    Article
    Cheng et al Plant Commun. 2023 Apr 13:100601. doi: 1
    Insert
    ecTadA8e-(GSAGSAAGSGEF)x3-dLbCas12a-D156R
    Use
    CRISPR; Gateway compatible ectada8e-(gsagsaagsgef…
    Tags
    5' and 3' NLS
    Expression
    Plant
    Promoter
    ZmUBI1
    Available Since
    May 17, 2023
    Availability
    Academic Institutions and Nonprofits only
    195569-plasmid-map-sequence-id-392989

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  25. pAAV-nEF Coff/Fon DREADD Gq-mCherry

    Plasmid
    #183534
    Purpose
    INTRSECT Chemogenetics - expresses DREADD Gq-mCherry in cells expressing Flp but not Cre
    Depositor
    Karl Deisseroth
    Insert
    DREADD Gq-mCherry
    Use
    AAV
    Mutation
    None
    Promoter
    nEF
    Available Since
    May 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    183534-plasmid-map-sequence-id-362029

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  28. PB-NEO-GFP-RFP

    Plasmid
    #157952
    Purpose
    seamless gene editing and component of Piggybac transposase system
    Depositor
    Yan-zhen Bi
    Type
    Empty backbone
    Use
    Mouse Targeting; Gene targeting
    Expression
    Mammalian
    Available Since
    Sept. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    157952-plasmid-map-sequence-id-310668

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Showing: 6451 - 6480 of 173176 results