We narrowed to 8,603 results for: sars

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FKBP-alpha(740-977)-GFP

Plasmid

#100731

Purpose

To express a clathrin "hook" in mammalian cells to hot-wire endocytosis (negative control)

Depositor

Stephen Royle

Article

Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

Insert

AP2A1 (Ap2a1 Mouse)

Tags

FKBP and GFP

Expression

Mammalian

Mutation

Long isoform

Promoter

CMV

Available Since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

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HA-HIF2α P531A-pBabe-puro

Plasmid

#26091

Depositor

William Kaelin

Article

Kondo et al Cancer Cell. 2002 Apr . 1(3):237-46.

Insert

Hypoxia inducible factor 2 alpha (EPAS1 Human)

Use

Retroviral

Tags

HA-tag

Expression

Mammalian

Mutation

cDNA with point mutation P531A

Available Since

Sept. 8, 2010

Availability

Academic Institutions and Nonprofits only

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pBMN mCherry-NDP52(V136S/C443K)

Plasmid

#119686

Purpose

For mammalian expression of LIR/UBD double mutant NDP52

Depositor

Michael Lazarou

Article

Padman et al Nat Commun. 2019 Jan 24;10(1):408. doi:

Insert

NDP52 (CALCOCO2 Human)

Use

Retroviral

Tags

mCherry

Expression

Mammalian

Mutation

V136S/C443K

Available Since

Jan. 30, 2019

Availability

Academic Institutions and Nonprofits only

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PVT1-luciferase

Plasmid

#113350

Purpose

evaluate FOXA1 DIV motif enhancer activity near 50 kb of PVT1 gene

Depositor

Ralf Jauch

Article

Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

Insert

PVT1-enhancer (PVT1 Human)

Use

Luciferase

Available Since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pHAGE2-EF1a-MCS-hPOT1_IRES-blast

Plasmid

#166411

Purpose

express hPOT1 in mammalian cells

Depositor

Agnel Sfeir

Article

Pinzaru et al Genes Dev. 2020 Dec 1;34(23-24):1619-163

Insert

hPOT1 (POT1 Human)

Use

Lentiviral

Tags

myc

Expression

Mammalian

Promoter

ef-1a

Available Since

May 10, 2021

Availability

Academic Institutions and Nonprofits only

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U6-Slc1a3 sgRNA; EF1a-dCas9-KRAB-GFP

Plasmid

#194283

Purpose

EF1a-dCas9-KRAB-GFP with Slc1a3 sgRNA

Depositor

Elizabeth Kirby

Article

Rieskamp et al iScience. 2023 Jun 8;26(7):107068. doi:

Insert

dCas9-KRAB-GFP

Use

CRISPR and Lentiviral

Tags

GFP

Promoter

EF1a

Available Since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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TetO-FUW-PLAGL1

Plasmid

#61542

Purpose

Expresses PLAGL1 in mammalian cells

Depositor

Rudolf Jaenisch

Article

Cassady et al Stem Cell Reports. 2014 Nov 6. pii: S221

Insert

PLAGL1 (PLAGL1 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Jan. 22, 2015

Availability

Academic Institutions and Nonprofits only
GFP-Tollip M240A,F241A

Plasmid

#178051

Purpose

GFP fused to human Tollip cDNA carrying mutations in the CUE domain. Localisation and expression in mammalian cells.

Depositor

David Tumbarello

Article

Ryan et al EMBO J. 2020 Jun 2;39(11):e102539. doi:

Insert

Toll-Interacting Protein (TOLLIP Human)

Tags

GFP

Expression

Mammalian

Mutation

Changed Methionine 240 to Alanine and changed Phe…

Promoter

CMV

Available Since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pBlastR-12xLexAOp-mCherry-MiniWhite

Plasmid

#165917

Purpose

Blasticidin resistant 12xLexAOp mCherry Mini-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screening

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Selectable LexAOp Response Vector

Use

Synthetic Biology

Available Since

April 30, 2021

Availability

Academic Institutions and Nonprofits only

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pMP71-mIL15-IL12rb1-sushi-il12rb2

Plasmid

#174606

Purpose

Expresses a constitutively activated murine IL-12 receptor tagged with extracellular myc and flag tags

Depositor

Stanley Riddell

Article

Veatch et al J Clin Invest. 2021 Aug 16;131(16):e1441

Insert

Expresses a constitutively activated murine IL-12 receptor tagged with extracellular myc and flag tags

Expression

Mammalian

Available Since

Oct. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pEDF-PhdRS

Plasmid

#127445

Purpose

Double mutant of wild type mmPylRS designed for incorporation of non-cannonical amino acids in to M13 bacteriophage

Depositor

Wenshe Liu

Article

Tharp et al Nat Commun. 2020 Mar 13;11(1):1392. doi:

Insert

Pyrrolysyl-tRNA synthetase/pyrrolysyl -tRNA pair

Expression

Bacterial

Mutation

N346A-C348A mutations on PylRS

Promoter

araBAD

Available Since

April 15, 2022

Availability

Academic Institutions and Nonprofits only

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pBMN mCherry-TAX1BP1(V142S/ΔUBD)

Plasmid

#119690

Purpose

For mammalian expression of LIR mutant TAX1BP1 without its UBD

Depositor

Michael Lazarou

Article

Padman et al Nat Commun. 2019 Jan 24;10(1):408. doi:

Insert

TAX1BP1 (TAX1BP1 Human)

Use

Retroviral

Tags

mCherry

Expression

Mammalian

Mutation

V142S & UBD deletion (1-755), See depositor c…

Available Since

Jan. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pLKO mouse ULK2 shRNA 93

Plasmid

#27635

Depositor

Reuben Shaw

Article

Egan et al Science. 2011 Jan 28;331(6016):456-61. d

Insert

mouse ULK2 shRNA 93

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

March 16, 2011

Availability

Academic Institutions and Nonprofits only
pET28a-His-hPKM2

Plasmid

#42515

Depositor

Dimitrios Anastasiou

Article

Anastasiou et al Science. 2011 Dec 2;334(6060):1278-83. d

Insert

Pyruvate Kinase M2 (PKM Human)

Tags

His

Expression

Bacterial

Available Since

March 7, 2013

Availability

Academic Institutions and Nonprofits only
PVT1-Tol2

Plasmid

#113381

Purpose

evaluate FOXA1 DIV motif enhancer activity near 50 kb of PVT1 gene

Depositor

Ralf Jauch

Article

Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

Insert

PVT1-enhancer (PVT1 Human)

Use

Tol2 reporter

Available Since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only
TetO-FUW-Hes5

Plasmid

#61536

Purpose

Expresses Hes5 in mammalian cells

Depositor

Rudolf Jaenisch

Article

Cassady et al Stem Cell Reports. 2014 Nov 6. pii: S221

Insert

Hes5 (Hes5 Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

Jan. 16, 2015

Availability

Academic Institutions and Nonprofits only
pET28a His6Flag2AUF1(p37)

Plasmid

#135939

Purpose

Expresses AUF1p37 in E. coli

Depositor

Yimon Aye

Article

Poganik et al FASEB J. 2019 Nov 1:fj201901930R. doi: 1

Insert

heterogeneous nuclear ribonucleoprotein D (HNRNPD Human)

Tags

Flag2 and His6

Expression

Bacterial

Promoter

T7

Available Since

Jan. 10, 2020

Availability

Academic Institutions and Nonprofits only

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p3XFlag-Mettl4

Plasmid

#86665

Purpose

Expresses murine Mettl4 containing N-terminal 3XFlag tag

Depositor

Douglas Feldman

Article

Kweon et al Mol Cell. 2019 Apr 5. pii: S1097-2765(19

Insert

Mettl4 (Mettl4 Mouse)

Tags

3xFlag

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 6, 2017

Availability

Academic Institutions and Nonprofits only

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pET28a His6Flag2AUF1(p45)

Plasmid

#135942

Purpose

Expresses AUF1p45 in E. coli

Depositor

Yimon Aye

Article

Poganik et al FASEB J. 2019 Nov 1:fj201901930R. doi: 1

Insert

heterogeneous nuclear ribonucleoprotein D (HNRNPD Human)

Tags

Flag2 and His6

Expression

Bacterial

Promoter

T7

Available Since

Jan. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pTE4254

Plasmid

#89050

Purpose

Expresses St chimeric gRNA and human codon-optimized StCas9

Depositor

Ervin Welker

Article

Toth et al Biol Direct. 2016 Sep 14;11:46. doi: 10.

Inserts

St chimeric gRNA
hStCas9

Use

CRISPR

Tags

SV40 NLS

Expression

Mammalian

Promoter

CMV and human U6

Available Since

April 14, 2017

Availability

Academic Institutions and Nonprofits only

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pAT15415-BEAR-GFP-target-mCherry

Plasmid

#162995

Purpose

plasmid expressing an sgRNA targeting the BEAR-GFP plasmid along with an mCherry marker

Depositor

Ervin Welker

Article

Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

Insert

sgRNA targeting the BEAR-GFP plasmid

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Sept. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pIRESneo2 mycBioID-Tollip

Plasmid

#178054

Purpose

myc-BioID fused to human Tollip cloned into the pIRESneo2 plasmid. For generation of stable expressing mammalian cells used for proximity dependent biotin labelling.

Depositor

David Tumbarello

Article

Ryan et al EMBO J. 2020 Jun 2;39(11):e102539. doi:

Insert

Toll-Interacting Protein (TOLLIP Human)

Tags

mycBioID

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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V5-bArr1

Plasmid

#201491

Purpose

Expression of the beta-arrestin1 containing a N-terminal V5 tag.

Depositor

Patrick Giguère

Article

Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

Insert

V5-bArr1 (ARRB1 Human)

Tags

V5

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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mCherry-MyosinIIC-N-18

Plasmid

#55108

Purpose

Localization: Cytoskeleton, Excitation: 587, Emission: 610

Depositor

Michael Davidson

Article

Ebrahim et al Curr Biol. 2013 Apr 22;23(8):731-6. doi:

Insert

MyosinIIC

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only
pU6-sgGAL4-4

Plasmid

#46916

Purpose

Human pSico-based U6 vector containing murine U6 promoter and sgRNA targeting GAL4 UAS promoter (negative control)

Depositor

Stanley Qi, Jonathan Weissman

Article

Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

Inserts

sgGAL4-4
Puromycin resistance and mCherry

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

CMV and U6

Available Since

Oct. 1, 2013

Availability

Academic Institutions and Nonprofits only

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p3xFlag-Alkbh4

Plasmid

#105240

Purpose

Expresses murine Alkbh4 containing an N-ternimal 3xFlag tag

Depositor

Douglas Feldman

Article

Kweon et al Mol Cell. 2019 Apr 5. pii: S1097-2765(19

Insert

Alkbh4 (Alkbh4 Mouse)

Tags

Flag and HA

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-dLight1.3a

Plasmid

#111055

Purpose

Expresses dLight1.3a in mammalian cells

Depositor

Lin Tian

Article

Patriarchi et al Science. 2018 Jun 29;360(6396). pii: sci

Insert

dLight1.3a

Tags

Flag tag

Expression

Mammalian

Promoter

CAG

Available Since

July 20, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a-DONSON

Plasmid

#208797

Purpose

Xenopus laevis His6-Myc-DONSON wild type

Depositor

Aga Gambus

Article

Cvetkovic et al Mol Cell. 2023 Nov 16;83(22):4017-4031.e

Insert

DONSON (donson.L Frog)

Tags

His and Myc

Expression

Bacterial

Promoter

T7

Available Since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pLJM1 p18 mito

Plasmid

#26638

Depositor

David Sabatini

Article

Sancak et al Cell. 2010 Apr 16. 141(2):290-303.

Insert

p18 mito (LAMTOR1 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Available Since

Oct. 26, 2010

Availability

Academic Institutions and Nonprofits only
pXPR_BRD003 sgKEAP1-2

Plasmid

#74986

Purpose

sgKEAP1-2, puromycin selection

Depositor

William Hahn

Article

Krall et al Elife. 2017 Feb 1;6:e18970. doi: 10.7554

Insert

KEAP1

Use

CRISPR

Expression

Mammalian

Available Since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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