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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 5,870 results for: SPI;

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Showing: 4301 - 4320 of 5870 results

  1. pJRH051

    Plasmid
    #171625
    Purpose
    2nd generation lentiviral transfer plasmid encoding a U6 promoter expressing a spyCas9 sgRNA (no spacer, BsmBI cutsites) and an EF1-a promoter expressing mNeonGreen
    Depositor
    Jennifer Doudna
    Article
    Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:
    Inserts
    spyCas9 sgRNA-BsmBI-Destination
    mNeon
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    EF1-a and U6
    Available Since
    June 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    171625-plasmid-map-sequence-id-335505

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  5. pYPQ230-RRV

    Plasmid
    #188543
    Purpose
    Gateway entry clone (attL1 & attR5) for LbCas12a-RRV mediated genome editing
    Depositor
    Yiping Qi
    Article
    Zhang et al Genome Biol. 2023 Apr 30;24(1):102. doi:
    Insert
    LbCas12a-RRV
    Use
    CRISPR; Gateway compatible lbcas12a-rrv entry clo…
    Tags
    NLS
    Expression
    Plant
    Mutation
    G146R, D156R, R182V
    Available Since
    May 17, 2023
    Availability
    Academic Institutions and Nonprofits only
    188543-plasmid-map-sequence-id-369473

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  16. L2092-GPA-FullBRET-Retrovirus-TD142

    Plasmid
    #222877
    Purpose
    Retroviral vector encoding positive control receptor chain for BRET signal. FRB ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, full Nanoluciferase, RH linker, CyOFP1.
    Depositor
    Joshua Leonard
    Article
    Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1
    Insert
    FRB-GPA-NanoLuc-CyOFP1
    Use
    Retroviral
    Tags
    Myc
    Expression
    Mammalian
    Promoter
    MSCV LTR
    Available Since
    Aug. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    222877-plasmid-map-sequence-id-441620

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  17. pCAT003

    Plasmid
    #171628
    Purpose
    2nd generation lentiviral transfer plasmid encoding a U6 promoter expressing a spyCas9 sgRNA targeting TRAC and an EF1-a promoter expressing mNeonGreen
    Depositor
    Jennifer Doudna
    Article
    Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:
    Inserts
    spyCas9 sgRNA-TRAC
    mNeon
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    CAG and EF1-a
    Available Since
    June 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    171628-plasmid-map-sequence-id-336485

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  20. pLGB36

    Plasmid
    #135621
    Purpose
    Suicide vector for allelic replacement in Bacteroides species including B. fragilis strain 638R, erythromycin selection and aTC-inducible ss-Bfe3 counterselection
    Depositor
    Laurie Comstock
    Article
    Ito et al mBio. 2020 Feb 4;11(1). pii: mBio.03238-
    Insert
    bfe3
    Use
    Bacteroides suicide vector with inducible counter…
    Tags
    signal sequence for periplasmic targeting
    Promoter
    Bacteroides promoter regualted by TetR transcript…
    Available Since
    Jan. 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    135621-plasmid-map-sequence-id-267063

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Showing: 4301 - 4320 of 5870 results