172,695 results
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Plasmid#49172PurposeBacterial expression of GFP nano bodyDepositorInsertGFP nanobody
TagsHHHHHHExpressionBacterial and MammalianPromoterT7Available SinceNov. 25, 2013AvailabilityAcademic Institutions and Nonprofits only -
The Mammalian Toolkit
Plasmid Kit#1000000180PurposePlasmids for Golden Gate-based assembly of cellular circuits for use in mammalian cell.DepositorApplicationCloning and Synthetic BiologyVector TypeMammalian ExpressionCloning TypeGolden GateAvailable SinceMarch 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV.hSyn.eGFP.WPRE.bGH
Plasmid#105539PurposeAAV expression of EGFP from hSyn promoterDepositorHas ServiceAAV1InsertEGFP
UseAAVExpressionMammalianPromoterhSynAvailable SinceMarch 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
LentiCRISPRv2-Opti
Plasmid#163126PurposeLentiCRISPRv2 variant with modified sgRNA scaffold from Chen et al. 2013 Cell.DepositorTypeEmpty backboneUseCRISPR and LentiviralAvailable SinceFeb. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYPQ136-tRNA2.0
Plasmid#158398PurposeGolden Gate entry vector to express the 6th gRNA with tRNA2.0 scaffold (with four MS2 binding sites) without promoterDepositorTypeEmpty backboneUseCRISPRExpressionPlantPromoterWithout promoterAvailable SinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pLifeAct-mScarlet3_N1
Plasmid#189767PurposeConstruct for labelling actin filaments in mammalian cells with lifeact-mScarlet3DepositorInsertLifeAct-mScarlet3
ExpressionMammalianPromoterCMVAvailable SinceMarch 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMXS-mitoGshF-blasticidin
Plasmid#210314PurposeExpresses mitochondrially targeted, humanized Streptococcus thermophilus GshF in mammalian cellsDepositorInsertBifunctional glutamate-cysteine ligase/glutathione synthetase GshF (gshAB Streptococcus thermophilus)
UseRetroviralTagsFLAG peptide, Homo sapiens ACO2 mitochondrial tar…MutationCodon optimized for expression in human cellsAvailable SinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1α-F-FLEX-mNaChBac-T2A-tdTomato (AAV8)
Viral Prep#60658-AAV8PurposeReady-to-use AAV8 particles produced from pAAV-EF1α-F-FLEX-mNaChBac-T2A-tdTomato (#60658). In addition to the viral particles, you will also receive purified pAAV-EF1α-F-FLEX-mNaChBac-T2A-tdTomato plasmid DNA. CAG-driven, Flp-dependent expression of the voltage-gated Na+ channel mNaChBac-T2A-tdTomato. These AAV preparations are suitable purity for injection into animals.DepositorPromoterEF1αAvailable SinceOct. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
Mito-B
Plasmid#209864PurposeDimerization dependent fluorescent protein B anchored to cytosolic face of the mitochondrial membrane with C-terminal targeting sequence of human MAVSDepositorInsertddGFP B
TagsTargeting domain of MAVS RPSPGALWLQVAVTGVLVVTLLVV…ExpressionMammalianPromoterCMVAvailable SinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMRX-IB-HaloTag7-mGFP-KDEL
Plasmid#184904PurposeStable expression of HaloTag7-mGFP-KDEL in mammalian cells to measure ER-phagy activityDepositorInsertKDEL
TagsHaloTag7 and monomeric EGFPExpressionMammalianAvailable SinceJune 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
Flag-SIRT1
Plasmid#1791PurposeMammalian expression of flag-tagged SIRT1DepositorAvailable SinceDec. 7, 2005AvailabilityAcademic Institutions and Nonprofits only -
PKC iota WT
Plasmid#21252DepositorAvailable SinceSept. 8, 2009AvailabilityAcademic Institutions and Nonprofits only -
pUAS-NanoLuc
Plasmid#87696PurposeGal4VP16 driven expression of NanoLucDepositorInsertNanoLuc
Tagsnuclear localization signalExpressionMammalianAvailable SinceApril 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDusk
Plasmid#43795PurposeEmpty backbone for blue-light-regulated expression of a gene of interest. Expression is repressed under blue illumination.DepositorTypeEmpty backboneExpressionBacterialAvailable SinceApril 9, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMV306G13+Lux
Plasmid#26160DepositorInsertG13 promoter + Bacterial luciferase operon
ExpressionBacterialMutationIt contains a gram-positive enhanced translation …Available SinceFeb. 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCDH-EF1-copGFP-T2A-Puro
Plasmid#72263PurposeExpress copGFP and puromycin resistance gene under EF1 promoterDepositorInsertcopGFP-T2A-Puro
UseLentiviralExpressionMammalianPromoterelongation factor-1Available SinceJan. 11, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET22b-csgA
Plasmid#89581PurposeExpress csgA curli protein with GS linker and HistaqDepositorAvailable SinceMay 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pKeima-LiveDrop
Plasmid#199695Purposelipophagy reporter system based on the Keima-fluorophoreDepositorAvailable SinceJan. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
tetO-MYOCD
Plasmid#170696Purposedoxycycline-inducible overexpression of MYOCDDepositorInsertMYOCD (MYOCD Human)
UseLentiviral; Doxycycline inducibleExpressionMammalianPromoterTRE promoter, Tet-ONAvailable SinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only