Skip to main content
Addgene
Showing: 6511 - 6540 of 8440 results

  3. LV TRE-GFP-TMD-DLL1-Nbox-mutant

    Plasmid
    #194937
    Purpose
    LV construct encoding a mutated GFP-TMD-DLL1 ligand containing an ablated Nbox site under dox-inducible TRE promoter and containing a PGK-rtTA-M2-IRES-Puro regulator/resistance marker cassette.
    Depositor
    John Ngo
    Article
    Sloas et al Nat Biotechnol. 2023 Jan 16. doi: 10.103
    Insert
    eGFP-(PDGFR)TMD-rDLL1 ICD-Nbox-AlaX3-mutant
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    194937-plasmid-map-sequence-id-386567

    Enlarge

  4. pSLS.539

    Plasmid
    #184958
    Purpose
    Test effect of a1/a2 length on editing fabH using retron recombineering
    Depositor
    Seth Shipman
    Article
    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d
    Insert
    Eco1 RT and recombineering ncRNA, fabH G954A, a1/a2 length: 12
    Use
    Tags
    Expression
    Bacterial
    Mutation
    fabH donor G954A
    Promoter
    T7/lac
    Available since
    Jan. 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    184958-plasmid-map-sequence-id-381448

    Enlarge

  6. pSCL.031

    Plasmid
    #184968
    Purpose
    Test effect of extending -Eco2 a1/a2 in yeast
    Depositor
    Seth Shipman
    Article
    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d
    Insert
    Eco2: RT and ncRNA(extended), a1/a2 length: 29
    Use
    Tags
    Expression
    Yeast
    Mutation
    human codon optimized RT, a1/a2 length extended t…
    Promoter
    Gal7
    Available since
    Nov. 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    184968-plasmid-map-sequence-id-376702

    Enlarge

  11. pSLS.544

    Plasmid
    #184961
    Purpose
    Test effect of a1/a2 length on editing murF using retron recombineering
    Depositor
    Seth Shipman
    Article
    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d
    Insert
    Eco1 RT and recombineering ncRNA, murF G1359A, a1/a2 length: 22
    Use
    Tags
    Expression
    Bacterial
    Mutation
    murF donor G1359A, a1/a2 length extended to 22 bp
    Promoter
    T7/lac
    Available since
    Nov. 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    184961-plasmid-map-sequence-id-376648

    Enlarge

  13. pSLS.543

    Plasmid
    #184960
    Purpose
    Test effect of a1/a2 length on editing murF using retron recombineering
    Depositor
    Seth Shipman
    Article
    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d
    Insert
    Eco1 RT and recombineering ncRNA, murF G1359A, a1/a2 length: 12
    Use
    Tags
    Expression
    Bacterial
    Mutation
    murF donor G1359A
    Promoter
    T7/lac
    Available since
    Nov. 2, 2022
    Availability
    Academic Institutions and Nonprofits only
    184960-plasmid-map-sequence-id-376545

    Enlarge

  15. pSLS.545

    Plasmid
    #184962
    Purpose
    Test effect of a1/a2 length on editing priB using retron recombineering
    Depositor
    Seth Shipman
    Article
    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d
    Insert
    Eco1 RT and recombineering ncRNA, priB G1069A, a1/a2 length: 12
    Use
    Tags
    Expression
    Bacterial
    Mutation
    priB donor G1069A
    Promoter
    T7/lac
    Available since
    Nov. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    184962-plasmid-map-sequence-id-376122

    Enlarge

  18. L1-AA027

    Plasmid
    #185752
    Purpose
    Level 1 loop assembly vector containing a transcriptional unit with a Hygromycin resistance cassette under the control of the A. agrestis EF1a promoter (pAaEF1a).
    Depositor
    Peter Szoevenyi
    Article
    Frangedakis et al New Phytol. 2021 Jun 2. doi: 10.1111/nph
    Insert
    HygR2
    Use
    Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    July 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    185752-plasmid-map-sequence-id-367857

    Enlarge

  19. pTS2341_Tier3(SB)-Puro

    Plasmid
    #169639
    Purpose
    Tier-3 vector for stable stable integration of up to three cassettes via sleeping beauty transposase including a PuroR resistance gene (5'ITR-A1-pA::A2-pA::PRPBSA-PuroR-pA-3'ITR)
    Depositor
    Martin Fussenegger
    Article
    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10
    Insert
    PRPBSA-driven PuroR expression
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    PRPBSA
    Available since
    July 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    169639-plasmid-map-sequence-id-366789

    Enlarge

  20. pTS2351_Tier3(SB)-Zeo

    Plasmid
    #169648
    Purpose
    Tier-3 vector for stable stable integration of up to three cassettes via sleeping beauty transposase including a ZeoR resistance gene (5'ITR-A1-pA::A2-pA::PRPBSA-ZeoR-pA-3'ITR)
    Depositor
    Martin Fussenegger
    Article
    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10
    Insert
    PRPBSA-driven ZeoR expression
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    PRPBSA
    Available since
    July 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    169648-plasmid-map-sequence-id-366198

    Enlarge

Showing: 6511 - 6540 of 8440 results