We narrowed to 36,948 results for: kan

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pEGFP-TDP-43

Plasmid

#190093

Purpose

Expresses TDP-43 in mammalian cells. Note: EGFP and TDP-43 are expressed in different reading frames.

Depositor

Bowen Liu

Publication

Liu et al Commun Biol. 2022 Aug 13;5(1):816. doi:

Insert

TAR DNA-Binding Protein 43 (TARDBP Human)

Expression

Mammalian

Available since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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phCMV-229E-D15

Plasmid

#188908

Purpose

express truncated 229E spike for pseudovirus production

Depositor

David Nemazee

Insert

229E spike (S 229E)

Expression

Mammalian

Mutation

c-terminal 15aa deletion

Promoter

CMV

Available since

Aug. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pH2B-miRFP670nano3

Plasmid

#184670

Purpose

Histone 2B labelled with near-infrared fluorescent protein miRFP670nano3

Depositor

Vladislav Verkhusha

Publication

Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

Insert

H2B-miRFP670nano3

Tags

miRFP670nano3

Expression

Mammalian

Promoter

CMV

Available since

July 25, 2022

Availability

Academic Institutions and Nonprofits only

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pFX-Tom20-ｍCherry

Plasmid

#174188

Purpose

Express mCherry with mitochondria-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Publication

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

mCherry with mitchondria-targeting sequence

Tags

the mitochondria-targeting sequence of TOM20

Expression

Mammalian

Promoter

CMV

Available since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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RLC11

Pooled library

#163955

Purpose

Targets open reading frames and non-coding RNAs in M. smegmatis.

Depositor

Jeremy Rock

Publication

Bosch et al Cell. 2021 Jul 16. pii: S0092-8674(21)00

Expression

Bacterial

Use

CRISPR

Available since

Oct. 1, 2021

Availability

Academic Institutions and Nonprofits only
psgRNAv1-empty

Plasmid

#171611

Purpose

sgRNA_v1 plasmid for AsCas12f1 in bacteria

Depositor

Quanjiang Ji

Publication

Wu et al Nat Chem Biol. 2021 Sep 2. pii: 10.1038/

Insert

AsCas12f1_sgRNA_1

Expression

Bacterial

Available since

Sept. 10, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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plRL58

Plasmid

#166886

Purpose

Optimized M. tuberculosis CRISPRi plasmid; contains an L5 attP region (no L5 Integrase) that allows for integration when co-transformed with an L5 Integrase delivery plasmid (e.g. plRL19, ID #163634)

Depositor

Jeremy Rock

Publication

Bosch et al Cell. 2021 Jul 16. pii: S0092-8674(21)00

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Promoter

Tet-On

Available since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

11 Articles

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pEGFP-BLM

Plasmid

#110299

Purpose

Mammalian expression of a EGFP-tagged full length Bloom's syndrome helicase

Depositor

Chris Kok-Lung Chan

Insert

BLM (Bloom's Syndrome) helicase (BLM Human)

Tags

EGFP

Expression

Mammalian

Available since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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Creb1

Plasmid

#154942

Purpose

Expresses Creb1 in mammalian cells

Depositor

Georgios Stathopoulos

Insert

Creb1 (Creb1 Mouse)

Expression

Mammalian

Available since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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PB-CAG-CasRX-P2A-EGFP-BGH PolyA

Plasmid

#154004

Purpose

Express CasRx

Depositor

Hui Yang

Publication

He et al Protein Cell. 2020 Mar 18. pii: 10.1007/

Insert

CasRx

Use

CRISPR

Promoter

CAG

Available since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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GFP-Trak1

Plasmid

#127621

Purpose

N-terminal GFP-tagged mouse Trak1

Depositor

Josef Kittler

Publication

Birsa et al J Biol Chem. 2014 May 23;289(21):14569-8

Insert

Trak1 (Trak1 Mouse)

Tags

eGFP

Expression

Mammalian

Available since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pmRuby3-24xGCN4

Plasmid

#132413

Purpose

overexpression in human cells

Depositor

Ling-Ling Chen

Publication

Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

Insert

mRuby3

Expression

Mammalian

Mutation

no

Available since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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ITPKA_3xmNeonGreen

Plasmid

#129606

Purpose

In vivo visualization of F-actin (can be used for colocalization studies)

Depositor

Dorus Gadella

Publication

Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

Insert

F-tractin

Expression

Mammalian

Promoter

CMV

Available since

Jan. 7, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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SpyTag003-Talin rod-mCherry

Plasmid

#133567

Purpose

Expresses SpyTag003-Talin rod-mCherry in mammalian cells

Depositor

Mark Howarth

Publication

Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

Insert

SpyTag003-Talin rod-mCherry

Expression

Mammalian

Promoter

CMV

Available since

Dec. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pHRE

Plasmid

#134909

Purpose

Overexpression of recombinant proteins in plants thanks to efficient translational efficiency. Empty vector, clone ORF using Bsa1 sites. Test UTR combinations by swapping 5' and 3' UTRs.

Depositor

George Lomonossoff

Publication

Peyret et al Plant Methods. 2019 Sep 18;15:108. doi:

Type

Empty backbone

Use

Synthetic Biology

Expression

Plant

Promoter

double 35S

Available since

Dec. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pMM113

Plasmid

#127210

Purpose

BeYDV replicon with constitutive Luc expression, WUS2 and AtSTM, sgRNA targeting PDS

Depositor

Daniel Voytas

Publication

Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

Insert

WUS2, AtSTM, sgRNA, Luc

Expression

Plant

Mutation

codon optimized CDSs

Available since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pMM136

Plasmid

#127217

Purpose

BeYDV replicon with constitutive Luc expression, MP-delta, sgRNA targeting PDS

Depositor

Daniel Voytas

Publication

Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

Insert

Luc, MP-delta, sgRNA

Expression

Plant

Mutation

codon optimized CDSs

Available since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-Rheb

Plasmid

#133767

Purpose

Expresses GFP-tagged Rheb in mammalian cells

Depositor

Shawn Ferguson

Publication

Angarola et al Mol Biol Cell. 2019 Oct 15;30(22):2750-2

Insert

Rheb (RHEB Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available since

Nov. 26, 2019

Availability

Academic Institutions and Nonprofits only

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phNFL

Plasmid

#132589

Purpose

Expresses human NFL

Depositor

Anthony Brown

Publication

Stone et al Cytoskeleton (Hoboken). 2019 Jul;76(7-8)

Insert

NEFL (NEFL Human)

Expression

Mammalian

Promoter

CMV

Available since

Nov. 20, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pENTR_L1-miniTurbo-YFP-NLS-STOP-L2

Plasmid

#127362

Purpose

gateway entry vector for expressing nuclear miniTurbo-YFP under a promoter of choice

Depositor

Dominique Bergmann

Publication

Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

Insert

miniTurbo (BirA mutant)

Use

Gateway Cloning

Tags

GS linker, NLS, V5, and YFP

Mutation

aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

Promoter

no promoter

Available since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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