We narrowed to 5,839 results for: pcas

1. pMPO 59

   Plasmid

   #68364

   Purpose

   Expression vectors for the salycilate cascade expression system (Pnah/NahR-Psal/XylS2).ApR, expression vector with rrnBT1T2-Pm-T7 SD sequence-MCSII-sspH2 signal peptide, ColE1 replication origin

   Depositor

   Eduardo Santero

   Article

   Medina et al PLoS One. 2011;6(8):e23055. doi: 10.1371

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   Oct. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMPO 52

   Plasmid

   #68359

   Purpose

   Expression vectors for the salycilate cascade expression system (Pnah/NahR-Psal/XylS2).ApR, expression vector with rrnBT1T2-Pm-T7 SD sequence-MCSII, ColE1 replication origin

   Depositor

   Eduardo Santero

   Article

   Medina et al PLoS One. 2011;6(8):e23055. doi: 10.1371

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   Oct. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pARD (pKD-G4)

   Plasmid

   #62683

   Purpose

   Mammalian expression of amiR-eGFP from the broadly active CAG promoter/enhancer

   Depositor

   Masato Ohtsuka

   Article

   Miura et al PLoS One. 2015 Aug 18;10(8):e0135919. do

   Insert

   amiR-eGFP419
   

   Use

   RNAi

   Expression

   Mammalian

   Available Since

   Aug. 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. NG-ABE8e

   Plasmid

   #138491

   Purpose

   A-to-G base editor

   Depositor

   David Liu

   Article

   Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

   Insert

   ecTadA(8e)-nSpCas9 (NG)
   

   Expression

   Mammalian

   Available Since

   March 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTRANS_221

   Plasmid

   #91115

   Purpose

   Transformation, Type: T-DNA, Plant Selection: 2x35S:npt II, Viral Replication: BeYDV

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Type

   Empty backbone

   Expression

   Plant

   Promoter

   35S

   Available Since

   Sept. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pH-ePPE

   Plasmid

   #183097

   Purpose

   For plant prime editing in rice plants

   Depositor

   Caixia Gao

   Article

   Zong et al Nat Biotechnol. 2022 Mar 24. doi: 10.103

   Insert

   Nls-nCas9-NC-nls-MLV-Nls
   

   Use

   CRISPR

   Expression

   Plant

   Mutation

   D10A in SpCas9

   Available Since

   April 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. ABE8e

   Plasmid

   #138489

   Purpose

   A-to-G base editor

   Depositor

   David Liu

   Article

   Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

   Insert

   ecTadA(8e)-nSpCas9
   

   Expression

   Mammalian

   Available Since

   March 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHL-sec

   Plasmid

   #99845

   Purpose

   Mammalian expression with secretion signal sequence

   Depositor

   Edith Yvonne Jones

   Article

   Aricescu et al Acta Crystallogr D Biol Crystallogr. 200

   Insert

   ephrin b2 (EFNB2 Human)
   

   Tags

   His

   Expression

   Mammalian

   Available Since

   Aug. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. HSP70-pp7i-mCh-UPG

   Plasmid

   #161011

   Purpose

   C-terminal mCherry fusion to Arabidopsis HSP70 with intronic PP7

   Depositor

   Krishna Niyogi

   Article

   Alamos et al Nat Plants. 2021 Aug;7(8):1037-1049. doi

   Insert

   PP7 and H2B-mScarlet
   

   Expression

   Plant

   Available Since

   Dec. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. Eef1a-1955/-1>nls::Cas9::nls

    Plasmid

    #59987

    Purpose

    Eef1a (EF1alpha) promoter driving nls::Cas9::nls

    Depositor

    Lionel Christiaen

    Article

    Stolfi et al Development. 2014 Nov;141(21):4115-20. d

    Insert

    nls::Cas9::nls
    

    Use

    CRISPR

    Mutation

    Reverted mutations from dCas9 to wiltdtype

    Promoter

    Ciinte.Eef1a (EF1alpha) -1955/-1

    Available Since

    Nov. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBECKP-km

    Plasmid

    #117235

    Purpose

    A cytidine deaminase-mediated base-editing plasmid in Klebsiella Pneumoniae

    Depositor

    Quanjiang Ji

    Article

    Wang et al Appl Environ Microbiol. 2018 Sep 14. pii

    Insert

    ncas9-BEC, fusion protein of rAPOBEC1 and spCas9 nickase
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Available Since

    Nov. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pX330-AAVS1

    Plasmid

    #227272

    Purpose

    Expresses SpCas9 and a sgRNA targeting the AAVS1 loci for knock-in.

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    sgRNA Targeting AAVS1 (AAVS1 Synthetic)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Nov. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. TrkB Acceptor

    Plasmid

    #83953

    Purpose

    FRET acceptor for TrkB mEGFP

    Depositor

    Ryohei Yasuda

    Article

    Harward et al Nature. 2016 Sep 28;538(7623):99-103. do

    Insert

    Phospholipase C gamma 1 (Plcg1 Mouse)
    

    Tags

    mRFP

    Expression

    Mammalian

    Available Since

    April 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJRH-1187 VSVGmut

    Plasmid

    #201913

    Purpose

    Expresses a VSV-G variant (K47Q R354A) defective for LDL-R receptor binding (CAG promoter)

    Depositor

    Jennifer Doudna

    Article

    Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

    Insert

    VSV-G (K47Q, R354A)
    

    Expression

    Mammalian

    Mutation

    K47Q, R354A

    Promoter

    CAG

    Available Since

    Aug. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. wtTDP43tdTOMATOHA

    Plasmid

    #28205

    Depositor

    Zuoshang Xu

    Article

    Yang et al PLoS One. 2010 . 5(12):e15878.

    Insert

    Tar DNA binding protein (TARDBP Human)
    

    Tags

    HA tag and tdtomato

    Expression

    Mammalian

    Available Since

    June 1, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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16. Gg 3kb Green opsin GFP

    Plasmid

    #72919

    Purpose

    green opsin promoter from chicken (Gallus gallus) gDNA chr26:4,504,913–4,501,931 in galGal4 driving GFP

    Depositor

    Joseph Corbo

    Article

    Enright et al J Comp Neurol. 2015 Mar 1;523(4):649-68.

    Insert

    chicken green opsin promoter
    

    Available Since

    Feb. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCRISPomyces-2

    Plasmid

    #61737

    Purpose

    Streptomyces expression of codon-optimized Cas9 and custom gRNA

    Depositor

    Huimin Zhao

    Article

    Cobb et al ACS Synth Biol. 2014 Dec 8.

    Inserts

    sSpCas9
    gRNA cassette
    

    Use

    CRISPR

    Expression

    Bacterial

    Mutation

    BbsI-flanked lacZ cassette inserted in place of s…

    Promoter

    gapdhp(EL) and rpsL(XC)-BbsI

    Available Since

    Jan. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCJ1021

    Plasmid

    #228761

    Purpose

    Plasmid expressing Cas9 and 2 gRNAs for mouse Ift88. Use for disruption of mouse Ift88 in cultured cells.

    Depositor

    Bradley Yoder

    Insert

    Ift88 gRNAs (Ift88 Mouse)
    

    Use

    CRISPR

    Tags

    3XFLAG and GFP

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    Feb. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. p2T-CMV-ABEmax-BlastR

    Plasmid

    #152989

    Purpose

    A-to-G base editor

    Depositor

    David Liu

    Article

    Arbab et al Cell. 2020 Jun 9. pii: S0092-8674(20)306

    Insert

    ABE7.10-nSpCas9
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMSCV-Cas9-2A-GFP-sgRNA

    Plasmid

    #124889

    Purpose

    Contains spCas9-2A-GFP and guide RNA scaffold in single retroviral vector

    Depositor

    Harvey Lodish

    Article

    Li et al Haematologica. 2016 Jun;101(6):e216-9. d

    Insert

    Cas9
    

    Use

    Retroviral

    Tags

    2A-GFP

    Promoter

    MSCV

    Available Since

    May 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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