We narrowed to 12,017 results for: pcdna

1. pcDNA3.1-nAChr-Alpha6-mCherry

   Plasmid

   #50480

   Purpose

   this report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRF

   Depositor

   Henry Lester

   Article

   Drenan et al Mol Pharmacol. 2008 Jan;73(1):27-41. Epu

   Insert

   nAChr-alpha6 (Chrna6 Mouse)
   

   Use

   Tags

   mCherry fusion in M3-M4 loop (after residue A405)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Jan. 10, 2014

   Availability

   Academic Institutions and Nonprofits only

2. pcDNA3-HA-PHLPP1 N-terminal truncation

   Plasmid

   #22404

   Purpose

   Expresses a HA tagged, N-terminal truncation of human PHLPP1 in mammalian cells

   Depositor

   Alexandra Newton

   Article

   Gao et al Mol Cell. 2005 Apr 1. 18(1):13-24.

   Insert

   PHLPP1 (PHLPP1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 29, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA-Nup37-EGFP-polyA

   Plasmid

   #104562

   Purpose

   Express Nup37-EGFP in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

   Depositor

   Yi Zhang

   Article

   Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

   Insert

   Nup37 (Nup37 Mouse)
   

   Use

   Tags

   EGFP tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA5/FRT EGFP-miniAID

   Plasmid

   #101714

   Purpose

   For amplification of a EGFP-miniAID for N-terminal tagging

   Depositor

   Andrew Holland

   Article

   Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

   Insert

   EGFP-miniAID
   

   Use

   Tags

   EGFP-miniAID

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-HCMVgH-8his (Merlin)

   Plasmid

   #136447

   Purpose

   Mammalian expression plasmid for HCMV glycoprotein H (Merlin strain) extracellular domain with 8his purification tag

   Depositor

   Erik Procko

   Article

   Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.

   Insert

   glycoprotein H (UL75 Human betaherpesvirus 5)
   

   Use

   Tags

   Short GGSG linker and 8his tag

   Expression

   Mammalian

   Mutation

   Extracellular domain (a.a. 1-716). Codon-optimize…

   Promoter

   CMV

   Available Since

   July 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA5-FRT/TO-N-mEGFP-PARP7

   Plasmid

   #178004

   Purpose

   Expresses N-terminally mEGFP-tagged PARP7

   Depositor

   Karla Feijs

   Article

   Weixler et al Life Sci Alliance. 2022 Nov 11;6(1):e202

   Insert

   PARP7 (TIPARP Human)
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. LARG mCherry sspB in pcDNA3.1

   Plasmid

   #90459

   Purpose

   RhoA mediated cell migration

   Depositor

   Narasimhan Gautam

   Article

   O'Neill et al Dev Cell. 2018 Jun 15. pii: S1534-5807(1

   Inserts

   LARG DH-PH (aa 766–1138) (ARHGEF12 Human)
   mCherry
   sspB
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   R73Q

   Promoter

   CMV

   Available Since

   June 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1-mCherry-CyrCR1.mutant

   Plasmid

   #128746

   Purpose

   Expresses mCherry in mammalian cells. ~200 nt of Cyrano with 5 point mutations in miR-7 site inserted in mCherry 3'UTR.

   Depositor

   David Bartel

   Article

   Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

   Insert

   Cyrano conserved region (CR1) with mutant miR-7 site (OIP5-AS1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   5 point mutations disrupting the miR-7 site

   Promoter

   CMV

   Available Since

   Aug. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1(+)/AUG-nLuc-3XFLAG

   Plasmid

   #127299

   Purpose

   Express 3xFLAG tagged nanoLuciferase (nLuc) from AUG start codon

   Depositor

   Jeremy Wilusz

   Article

   Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

   Insert

   nanoLuciferase
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   June 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3 Flag TSC2 (S939A)

    Plasmid

    #14132

    Depositor

    Brendan Manning

    Article

    Manning et al Mol Cell. 2002 Jul . 10(1):151-62.

    Insert

    TSC2 S939A (TSC2 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    S939A.

    Promoter

    Available Since

    March 2, 2007

    Availability

    Academic Institutions and Nonprofits only

11. pcDNA3.1-N-Myc_1-560 USP7

    Plasmid

    #131244

    Purpose

    Mammalian expression of N-terminally Myc-tagged USP7, minus the UBL domains

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

    Insert

    Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Expresses USP7 amino acids 1 - 560. This truncati…

    Promoter

    Available Since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA-NLS-PhoCl-mCherry

    Plasmid

    #87691

    Purpose

    Express NLS-PhoCl-mCherry in mammalian cells

    Depositor

    Robert Campbell

    Article

    Zhang et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

    Insert

    NLS-PhoCl-mCherry
    

    Use

    Tags

    nuclear localization signal

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA-Dest47- C11orf83-GFP

    Plasmid

    #65844

    Purpose

    Mammalian expression of C terminally GFP-tagged C11orf83/UQCC3

    Depositor

    Amos Bairoch

    Article

    Desmurs et al Mol Cell Biol. 2015 Apr;35(7):1139-56. d

    Insert

    UQCC3 WT (UQCC3 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available Since

    July 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1(-) MID49 4xMyc Hisx6

    Plasmid

    #44596

    Depositor

    David Chan

    Article

    Loson et al Mol Biol Cell. 2013 Jan 2.

    Insert

    MiD49 (Mief2 Mouse)
    

    Use

    Tags

    4xMyc and 6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 24, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1-CarO-BFP2-TSERex

    Plasmid

    #124795

    Purpose

    Expresses the proton pump rhodopsin in mammalian cells under CAG promoter

    Depositor

    Yulong Li

    Article

    Wu et al Elife. 2019 Jan 14;8. pii: 43366. doi: 1

    Insert

    CarO-BFP2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    May 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1-Mac-BFP2-TSERex

    Plasmid

    #124793

    Purpose

    Expresses the proton pump rhodopsin in mammalian cells under CAG promoter

    Depositor

    Yulong Li

    Article

    Wu et al Elife. 2019 Jan 14;8. pii: 43366. doi: 1

    Insert

    Mac-BFP2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    May 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCDNA5/FRT-ChemoL-NAD

    Plasmid

    #193827

    Purpose

    CMV overexpression of ChemoL-NAD in mammalian cell lines

    Depositor

    Julien Hiblot

    Article

    Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

    Insert

    ChemoL-NAD
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    EGFP:A206K, T225R; HT7: L271E

    Promoter

    CMV

    Available Since

    June 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA4-TO-Hygromycin-mVenus-MAP

    Plasmid

    #44099

    Depositor

    Dannel McCollum

    Article

    Ma et al Mol Cell Proteomics. 2012 Aug;11(8):501-

    Type

    Empty backbone

    Use

    Tags

    mVenus-MAP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1-CD81-(TEV)-Cre

    Plasmid

    #167939

    Purpose

    human CD81-TEV cleavage site-Cre

    Depositor

    Masaharu Somiya

    Article

    Somiya et al J Extracell Vesicles. 2021 Nov;10(13):e1

    Insert

    human CD81 (CD81 Human)
    

    Use

    Tags

    Cre recombinase and TEV protease cleavage site

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCDNA-3xFLAG-NLS-TPP1

    Plasmid

    #53585

    Purpose

    Mammalian expression of 3xFLAG tagged, nuclear localized TPP1, N-terminal.

    Depositor

    Steven Artandi

    Article

    Zhong et al Cell. 2012 Aug 3;150(3):481-94. doi: 10.

    Insert

    TPP1 (ACD Human)
    

    Use

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    Silent mutation to eliminate PacI site

    Promoter

    CMV

    Available Since

    June 9, 2014

    Availability

    Academic Institutions and Nonprofits only