161,271 results

1. CRY2high-mCherry

   Plasmid

   #104063

   Purpose

   Expresses fusion of CRY2high mutant (CRY2PHR E490R) with mCherry

   Depositor

   Bianxiao Cui

   Article

   Duan et al Nat Commun. 2017 Sep 15;8(1):547. doi: 1

   Insert

   CRY2high-mCherry
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   CRY2PHR E490R

   Promoter

   CMV

   Available since

   Dec. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-CAG-mTurquoise2

   Plasmid

   #99122

   Purpose

   An AAV genome that ubiquitously expresses the fluorescent protein mTurquoise2 from the CAG promoter

   Depositor

   Viviana Gradinaru

   Article

   Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

   Insert

   mTurquoise2
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   July 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCIneo-lambdaN-HA-HsEDC4

   Plasmid

   #66597

   Purpose

   Expresses lambdaN-HA-tagged EDC4 in mammalian cells

   Depositor

   Elisa Izaurralde

   Article

   Tritschler et al Proc Natl Acad Sci U S A. 2009 Dec 22. 1

   Insert

   EDC4 (EDC4 Human)
   

   Use

   Tags

   lambdaN-HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. Integrin β4-GFP

   Plasmid

   #205092

   Purpose

   Express GFP-tagged human integrin β4 in mammalian cells

   Depositor

   Bianxiao Cui

   Article

   Zhang et al Nat Cell Biol. 2023 Sep 28. doi: 10.1038

   Insert

   Integrin beta 4 (ITGB4 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCDH-EF1-copGFP-T2A-Puro

   Plasmid

   #72263

   Purpose

   Express copGFP and puromycin resistance gene under EF1 promoter

   Depositor

   Kazuhiro Oka

   Insert

   copGFP-T2A-Puro
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   elongation factor-1

   Available since

   Jan. 11, 2016

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pcDNA3-CXCL12-sfGFP

   Plasmid

   #98961

   Purpose

   Mammalian expression plasmid for superfolder GFP-fused human chemokine CXCL12

   Depositor

   Erik Procko

   Article

   Heredia et al J Immunol. 2018 Apr 20. pii: jimmunol.18

   Insert

   CXCL12 (CXCL12 Human)
   

   Use

   Tags

   Superfolder GFP

   Expression

   Mammalian

   Mutation

   Full-length, wildtype

   Promoter

   CMV

   Available since

   April 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV.CAG-FLEX.SF-iGluSnFR.A184S

   Plasmid

   #106186

   Purpose

   Tight affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

   Depositor

   Loren Looger

   Article

   Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

   Insert

   SF-iGluSnFR.A184S
   

   Use

   AAV

   Tags

   Expression

   Mutation

   GltI: A184S

   Promoter

   CAG

   Available since

   July 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPGKPhiC31obpA

   Plasmid

   #13795

   Purpose

   Expression of mouse codon-optimized PhiC31 recombinase with bovine growth hormone polyadenylation (bpA) sequence.

   Depositor

   Philippe Soriano

   Article

   Raymond et al PLoS ONE. 2007 . 2():e162.

   Insert

   PhiC31o
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 16, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCFD5_w

   Plasmid

   #112645

   Purpose

   Ubiquitous expression of one or multiple sgRNAs in Drosophila. Selection marker: Mini-white.

   Depositor

   Michael Boutros

   Insert

   U6:3-tRNA-sgRNA expression cassette
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Aug. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. CMV-mito-Citron1

    Plasmid

    #134305

    Purpose

    mitochondria-targeted direct-response citrate sensor

    Depositor

    Robert Campbell

    Article

    Zhao et al ACS Cent Sci. 2020 Aug 26;6(8):1441-1450

    Insert

    Citron1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pS648∙CsR

    Plasmid

    #197860

    Purpose

    Expresses Streptococcus pyogenes' cas9 and a tailored sgRNA for counterselection in gram-negative bacteria

    Depositor

    Susanne Häußler

    Article

    Pankratz et al Nat Protoc. 2023 Oct 5. doi: 10.1038/s41

    Insert

    gRNA scaffold - Gm resistance cassette - incP oriT
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    PEM7

    Available since

    Oct. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. SaCas9_sgRNA_expression_in_pBluescript

    Plasmid

    #122090

    Purpose

    U6 driven SaCas9 sgRNA expression vector for cloning own guides

    Depositor

    Scot Wolfe

    Article

    Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

    Insert

    SaCas9 tracr (NEWENTRY Synthetic, S. pyogenes)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA1 Flag ATXN1[30Q]

    Plasmid

    #33236

    Depositor

    Huda Zoghbi

    Article

    Skinner et al Nature. 1997 Oct 30;389(6654):971-4.

    Insert

    Ataxin-1 (ATXN1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    insert contains a stretch of 29 amino acids (27 Q…

    Promoter

    CMV

    Available since

    July 18, 2014

    Availability

    Academic Institutions and Nonprofits only

14. Lenti-EFS-BirAopt-GSQ-RBXN-P2A-BLAST

    Plasmid

    #208048

    Purpose

    Enables constitutive expression of BirAopt alone; RBXN represents a EcoR1-BsiW1-Xba1-Not1 polylinker; to perform bioE3; selection with blasticidin

    Depositor

    James Sutherland, Rosa Barrio

    Article

    Barroso-Gomila et al Nat Commun. 2023 Nov 23;14(1):7656. doi:

    Insert

    BirAopt
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    BirAopt is human-codon-optimized BirA (PMID 15707…

    Promoter

    EFS

    Available since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAM5411

    Plasmid

    #132663

    Purpose

    RSF1010-based broad host-range plasmid with RK2 bom site and pUC origin expressing YFP and Sp/Sm resistance

    Depositor

    Susan Golden

    Article

    Bishe et al iScience. 2019 Oct 25;20:216-228. doi: 1

    Inserts

    aadA
    YFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PaadA and PconII

    Available since

    Nov. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pWPI-TBX2

    Plasmid

    #185526

    Purpose

    Constitutive overexpression of human TBX2

    Depositor

    Sean Palecek, Eric Shusta

    Article

    Gastfriend et al Sci Adv. 2024 Feb 2;10(5):eadi1737. doi:

    Insert

    TBX2 (TBX2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF-1a

    Available since

    Feb. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. PB-GFP11x7-HygR

    Plasmid

    #223676

    Purpose

    To check the split GFP signals in mitochondria (2)

    Depositor

    Juan Melero-Martin

    Article

    Lin et al Nature. 2024 May;629(8012):660-668. doi:

    Insert

    GFP11x7
    

    Use

    Tags

    GFP11

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. 7TGP

    Plasmid

    #24305

    Depositor

    Roel Nusse

    Article

    Fuerer et al PLoS One. 2010 . 5(2):e9370.

    Insert

    7xTcf-eGFP // SV40-PuroR
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    EGFP is GFP with S65T and F64L mutations

    Promoter

    Available since

    April 5, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLVX-M-Flag-BAP1

    Plasmid

    #125840

    Purpose

    express wild type BAP1

    Depositor

    Boyi Gan

    Article

    Zhang et al Nat Cell Biol. 2018 Oct;20(10):1181-1192

    Insert

    BAP1 (BRCA1 associated protein 1) (BAP1 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mutation

    Promoter

    Available since

    June 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pRDA_221

    Plasmid

    #169142

    Purpose

    Constitutive expression of EGFP and sgRNAs targeted to EGFP for measurement of activity of AsCas12a\EnAsCas12a.

    Depositor

    David Root, John Doench

    Insert

    EGFP , EGFP sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pME-QF2

    Plasmid

    #83307

    Purpose

    codes for QF2 (truncated QF) in a gateway middle entry backbone.

    Depositor

    Marnie Halpern

    Insert

    QF
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pJFRC12-10XUAS-IVS-myr::GFP

    Plasmid

    #26222

    Depositor

    Gerald Rubin

    Article

    Pfeiffer et al Genetics. 2010 Aug 9. ():.

    Insert

    myr::GFP
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 19, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCDH-puro-CMV-VC3AI

    Plasmid

    #78907

    Purpose

    Indicator of caspase-3-like protease activity

    Depositor

    Binghui Li

    Article

    Zhang et al Nat Commun. 2013;4:2157. doi: 10.1038/nc

    Insert

    VC3AI
    

    Use

    Lentiviral

    Tags

    HA and Myc

    Expression

    Mutation

    S320A, deletion of AA1-200

    Promoter

    CMV

    Available since

    June 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pEGFP-N3 alpha-SSeCKS

    Plasmid

    #61294

    Purpose

    Mammalian expression of GFP-alpha-SSeCKS

    Depositor

    Irwin Gelman

    Insert

    alpha SSeCKS (Akap12 Rat)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 9, 2014

    Availability

    Academic Institutions and Nonprofits only

25. pCDNA5/FRT_CMV_NES-Caprola_on-mEGFP

    Plasmid

    #194686

    Purpose

    CMV driven expression of the consitutively active calcium recorder positive control Caprola_on in mammalian cell lines fused to mEGFP

    Depositor

    Kai Johnsson

    Article

    Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

    Insert

    Caprola_on-mEGFP
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pGEP163

    Plasmid

    #137881

    Purpose

    PiggyBac donor vector for expression of mRFP-T2A-BlasticidinR

    Depositor

    Lindy Barrett

    Article

    Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

    Insert

    mRFP-T2A-BlasticidinR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pHR-mCh-Cry2WT

    Plasmid

    #101221

    Purpose

    Blue-light dependent optogenetic protein Cry2

    Depositor

    Clifford Brangwynne

    Article

    Shin et al Cell. 2017 Jan 12;168(1-2):159-171.e14.

    Insert

    Cry2PHR
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    Feb. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. scAAV-Cre

    Plasmid

    #177933

    Purpose

    Expresses Cre-recombinase and can be packaged into AAV particles.

    Depositor

    Roland Rad

    Article

    Kaltenbacher et al Nat Protoc. 2022 Apr;17(4):1142-1188. do

    Insert

    CAG-Cre
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAAV-synapsin-HaloCaMP1a-EGFP

    Plasmid

    #138327

    Purpose

    chemigenetic calcium indicator

    Depositor

    Eric Schreiter

    Article

    Deo et al Nat Chem Biol. 2021 Jun;17(6):718-723. d

    Has Service

    AAV1 and AAV9

    Insert

    HaloCaMP1a
    

    Use

    AAV

    Tags

    EGFP, His-tag, and Nuclear Exclusion Signal

    Expression

    Mammalian

    Mutation

    Promoter

    synapsin

    Available since

    March 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pcDNA Flag Yap1

    Plasmid

    #18881

    Depositor

    Yosef Shaul

    Article

    Levy et al Mol Cell. 2008 Feb 15. 29(3):350-61.

    Insert

    Yap1 (YAP1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 14, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pAAV_hsyn_NES-his-CaMPARI2-WPRE-SV40 (AAV1)

    Viral Prep

    #101060-AAV1

    Purpose

    Ready-to-use AAV1 particles produced from pAAV_hsyn_NES-his-CaMPARI2-WPRE-SV40 (#101060). In addition to the viral particles, you will also receive purified pAAV_hsyn_NES-his-CaMPARI2-WPRE-SV40 plasmid DNA. Synapsin-driven, photoconvertible (irreversible green-to-red) calcium integrator his-tagged CaMPARI2. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Eric Schreiter

    Article

    Moeyaert et al Nat Commun. 2018 Oct 25;9(1):4440. doi:

    Promoter

    Syn

    Tags

    None

    Available since

    July 23, 2018

    Availability

    Academic Institutions and Nonprofits only

32. mCherry-PCNA

    Plasmid

    #215117

    Purpose

    Expresses mCherry-PCNA in mammalian cells from a lentiviral vector.

    Depositor

    Michele Pagano

    Article

    Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

    Insert

    PCNA (PCNA Human)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pET246-pUC57 12xMS2V6

    Plasmid

    #104390

    Purpose

    MS2V6 cloning

    Depositor

    Robert Singer, Evelina Tutucci

    Article

    Tutucci et al Nat Methods. 2017 Nov 13. doi: 10.1038/n

    Insert

    12xMS2V6
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    All Loops are U-variants, interspaced by 50 nucle…

    Promoter

    Available since

    Dec. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pHR-TRE3G-dCas9-EGFP-HaloTag

    Plasmid

    #169451

    Purpose

    expression of dCas9-EGFP-HaloTag

    Depositor

    Stanley Qi

    Article

    Wang et al Cell. 2018 Nov 15;175(5):1405-1417.e14.

    Insert

    dCas9-EGFP-HaloTag
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. GFP-Rab4B

    Plasmid

    #61801

    Purpose

    Human Rab4B fluorescently tagged with GFP on the N-terminus for mammalian expression

    Depositor

    Gia Voeltz

    Article

    Rowland et al Cell. 2014 Nov 20;159(5):1027-41. doi: 1

    Insert

    Rab4B (RAB4B Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    None

    Promoter

    CMV

    Available since

    Jan. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pMSGV-mCD105scFv-myc-CD28HTMcostim-T2A-Thy1.1

    Plasmid

    #193942

    Purpose

    a fully murine CAR targeting CD105 (endoglin), a component of the TGFβ receptor expressed on the surface of certain solid tumors and acute leukemias.

    Depositor

    Greg Delgoffe

    Article

    Lontos et al Oncoimmunology. 2022 Oct 18;11(1):213122

    Insert

    heavy- and light-chain regions of the MJ7/18 hybridoma and constructed a single chain variable fragment (scFv)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pMThy1.1_hDDX5_FL

    Plasmid

    #88873

    Purpose

    constitutive expression of DDX5 in mammalian cells

    Depositor

    Dan Littman

    Article

    Huang et al Nature. 2015 Dec 24;528(7583):517-22. do

    Insert

    DDX5 (DDX5 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    MSCV

    Available since

    June 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pRsetB-pHRed

    Plasmid

    #31472

    Depositor

    Gary Yellen

    Article

    Tantama et al J Am Chem Soc. 2011 Jul 6. 133(26):10034

    Insert

    pHRed
    

    Use

    Tags

    7xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 21, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pAAV-FLEX-SaCas9-U6-sgSlc18a2

    Plasmid

    #124849

    Purpose

    Mutagenesis of Slc18a2

    Depositor

    Larry Zweifel

    Article

    Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

    Insert

    Slc18a2 (Slc18a2 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pLV-EF1a-CRISPRoffv2.1-IRES-BlastR

    Plasmid

    #207180

    Purpose

    Lentiviral Expression Plasmid for CRISPRoff2.1 driven by EF1a Promoter

    Depositor

    David Bersten

    Article

    Allen et al bioRxiv 2023.11.21.568191

    Insert

    CRISPRoffv2.1
    

    Use

    CRISPR and Lentiviral

    Tags

    HA and TagBFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    April 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge