Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

Filter By

282,636 results

Showing: 8721 - 8760 of 282636 results

  1. pAAV-S5E2-GCaMP6f (AAV PHP.eB)

    Viral Prep
    #135632-PHPeB
    Purpose
    Ready-to-use AAV PHP.eB particles produced from pAAV-S5E2-GCaMP6f (#135632). In addition to the viral particles, you will also receive purified pAAV-S5E2-GCaMP6f plasmid DNA. Expression of GCaMP6f under the control of the E2 regulatory element. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Jordane Dimidschstein
    Article
    Vormstein-Schneider et al Nat Neurosci. 2020 Dec;23(12):1629-1636.
    Promoter
    Tags
    None
    Available since
    Sept. 13, 2021
    Availability
    Academic Institutions and Nonprofits only

  2. CaMKII-somBiPOLES-mCerulean (AAV9)

    Viral Prep
    #154948-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from CaMKII-somBiPOLES-mCerulean (#154948). In addition to the viral particles, you will also receive purified CaMKII-somBiPOLES-mCerulean plasmid DNA. CamKII-driven expression of soma-targeted BiPOLES for optogenetic inhibition (blue light) and activation (red light). These AAV preparations are suitable purity for injection into animals.
    Depositor
    Simon Wiegert
    Article
    Vierock et al Nat Commun. 2021 Jul 26;12(1):4527. doi:
    Promoter
    CaMKIIa(0.4)
    Tags
    mCerulean
    Available since
    Sept. 1, 2021
    Availability
    Academic Institutions and Nonprofits only

  8. qTAG-AAVS1-Ef1a-Puro-moxGFP

    Plasmid
    #227273
    Purpose
    AAVS1 targeting donor for the insertion of Puro and a strong EF1a promoter expressing moxGFP. To be co-transfected with sgRNA plasmid px330-AAVS1 (Addgene #227272)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    AAVS1 Homology Arms flanking a 2A-Puro-EF1a-moxGFP cassette (AAVS1 Synthetic)
    Use
    CRISPR; Donor template
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Promoterless
    Available since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    227273-plasmid-map-sequence-id-452031

    Enlarge

  10. pAAV-syn-flex-iGluSnFR4s-NGR-WPRE

    Plasmid
    #234441
    Purpose
    AAV-mediated expression of glutamate sensor with improved sensitivity and slow deactivation kinetics (4s = slow); NGR vector matches or outperforms PDGFR in all conditions tested.
    Depositor
    GENIE Project
    Article
    Aggarwal et al bioRxiv 2025.03.20.643984
    Insert
    iGluSnFR4s-NGR
    Use
    AAV and Cre/Lox
    Tags
    IgK chain and Myc epi tag-NGR GPI anchor
    Expression
    Mammalian
    Mutation
    Promoter
    Synapsin
    Available since
    March 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    234441-plasmid-map-sequence-id-465749

    Enlarge

  11. pAAV-syn-iGluSnFR4s-NGR-WPRE

    Plasmid
    #234437
    Purpose
    AAV-mediated expression of glutamate sensor with improved sensitivity and slow deactivation kinetics (4s = slow); NGR vector matches or outperforms PDGFR in all conditions tested.
    Depositor
    GENIE Project
    Article
    Aggarwal et al bioRxiv 2025.03.20.643984
    Insert
    iGluSnFR4s-NGR
    Use
    AAV
    Tags
    IgK chain and Myc epi tag-NGR GPI anchor
    Expression
    Mammalian
    Mutation
    Promoter
    Synapsin
    Available since
    March 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    234437-plasmid-map-sequence-id-465724

    Enlarge

  13. nuclear NAD+ biosensor

    Plasmid
    #186789
    Purpose
    NAD+ biosensor comprised of a circularly permuted Venus fluorescent protein (cpVenus), a bipartite NAD+-binding domain modeled from bacterial DNA ligase, and a localization tag to target the nucleus
    Depositor
    Michael Cohen
    Article
    Cambronne et al Science. 2016 Jun 17;352(6292):1474-7. d
    Insert
    nuclear NAD+ biosensor
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    186789-plasmid-map-sequence-id-368062

    Enlarge

  22. iRFP-H2A

    Plasmid
    #158691
    Purpose
    This plasmid encodes an iRFP fused to H2A histone nuclear marker. It has CMV promoter driving the expression of iRFP fused to H2A. It has no selection markers
    Depositor
    Ravid Straussman
    Article
    Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:
    Insert
    iRFP670
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    May 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    158691-plasmid-map-sequence-id-311047

    Enlarge

  30. pAAV-hsyn-GRAB_5-HT1.0 (AAV9)

    Viral Prep
    #140552-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pAAV-hsyn-GRAB_5-HT1.0 (#140552). In addition to the viral particles, you will also receive purified pAAV-hsyn-GRAB_5-HT1.0 plasmid DNA. Synapsin-driven expression of GRAB_5-HT1.0 sensor. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Yulong Li
    Article
    Wan et al Nat Neurosci. 2021 Apr 5. pii: 10.1038/s
    Promoter
    Syn
    Tags
    None
    Available since
    Aug. 31, 2021
    Availability
    Academic Institutions and Nonprofits only

  32. pME-Cas9-T2A-GFP

    Plasmid
    #63155
    Purpose
    Middle Entry clone for Gateway containing a zebrafish codon-optimized Cas9 flanked by 2 NLS and followed by inframe GFP. Cas9 and GFP are separated by the sequence of a T2A self-cleaving peptide.
    Depositor
    Leonard Zon
    Article
    Ablain et al Dev Cell. 2015 Mar 4. pii: S1534-5807(15
    Insert
    Cas9-T2A-GFP
    Use
    CRISPR; Tol2 middle entry vector for gateway
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 1, 2015
    Availability
    Academic Institutions and Nonprofits only
    63155-plasmid-map-sequence-id-220487

    Enlarge

  35. pLL3.7-EF-EYFP-YAP1_5SA-PolyA

    Plasmid
    #112285
    Purpose
    Fluorescently labels human YAP1-2gamma isoform (504 aa) 5SA active mutant - serines 61, 109, 127, 164 and 397 (also known as 381 in other isoforms)
    Depositor
    Erik Sahai
    Article
    Ege et al Cell Syst. 2018 Jun 27;6(6):692-708.e13.
    Insert
    EYFP-YAP1 (YAP1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Human YAP1-2gamma with 5 Serines (61, 109, 127, 1…
    Promoter
    EF-1 alpha
    Available since
    May 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    112285-plasmid-map-sequence-id-227437

    Enlarge

  37. AAV-hSyn-Soma-jGCaMP8s (AAV9)

    Viral Prep
    #169256-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from AAV-hSyn-Soma-jGCaMP8s (#169256). In addition to the viral particles, you will also receive purified AAV-hSyn-Soma-jGCaMP8s plasmid DNA. hSyn-driven expression of soma-targeted calcium sensor GCaMP8s (more sensitive). These AAV preparations are suitable purity for injection into animals.
    Depositor
    Marianne Fyhn
    Article
    Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1
    Promoter
    Tags
    None
    Available since
    Feb. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
Showing: 8721 - 8760 of 282636 results