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pCas9-polyA

Plasmid

#72602

Purpose

express hCas9 byT7 promoter with polyadenine tails

Depositor

Tomoji Mashimo

Article

Yoshimi et al Nat Commun. 2016 Jan 20;7:10431. doi: 10

Insert

Cas9-polyadenine

Tags

81-bp polyadenylation

Promoter

T7

Available Since

July 15, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Tat HA (P#29)

Plasmid

#14654

Depositor

Matija Peterlin

Article

Cujec et al Genes Dev. 1997 Oct 15. 11(20):2645-57.

Insert

Tat (tat HIV)

Tags

HA

Expression

Mammalian

Available Since

June 30, 2008

Availability

Academic Institutions and Nonprofits only

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pLKO-RFP-shCntrl

Plasmid

#69040

Purpose

Lentiviral expression of control shRNA

Depositor

William Kaelin

Article

Lu et al Science. 2014 Jan 17;343(6168):305-9. do

Insert

control shRNA

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

U6

Available Since

Oct. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-GFAP104-ChR2-mCherry

Plasmid

#58892

Purpose

AAV-mediated expression of ChR2-mCherry under the GFAP promoter

Depositor

Edward Boyden

Article

Perea et al Nat Commun. 2014;5:3262. doi: 10.1038/nc

Has Service

AAV5

Insert

ChR2-mCherry

Use

AAV

Tags

mCherry

Expression

Mammalian

Promoter

GFAP104

Available Since

Aug. 14, 2014

Availability

Academic Institutions and Nonprofits only

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pDD363

Plasmid

#91829

Purpose

LoxP-flanked SEC donor for the SapTrap cloning system

Depositor

Bob Goldstein

Article

Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

Insert

LoxP-SEC-LoxP

Use

CRISPR and Cre/Lox

Expression

Worm

Available Since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFP-Rab21

Plasmid

#83421

Purpose

Mouse Rab21 (wild type)

Depositor

Johanna Ivaska

Article

Pellinen et al J Cell Biol. 2006 Jun 5;173(5):767-80.

Insert

Rab21 (Rab21 Mouse)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only

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stathmin-GFP

Plasmid

#86782

Purpose

Mammalian expression of stathmin-GFP

Depositor

Lynne Cassimeris

Article

Ringhoff et al Mol Biol Cell. 2009 Aug;20(15):3451-8. d

Insert

stathmin (STMN1 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pLB1080

Plasmid

#37826

Depositor

Ann Hubbard

Article

Kallay et al J Cell Biochem. 2006 Oct 1;99(2):647-64.

Insert

ATP7B (ATP7B Human)

Tags

mEGFP

Promoter

CMV

Available Since

Nov. 14, 2013

Availability

Academic Institutions and Nonprofits only

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pX330A_dCas9-1x4

Plasmid

#63598

Purpose

Expresses dCas9 and gRNA

Depositor

Takashi Yamamoto

Article

Nakagawa et al BMC Biotechnol. 2015 May 22;15(1):33.

Insert

humanized S. pyogenes dCas9

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Promoter

CBh

Available Since

June 1, 2015

Availability

Academic Institutions and Nonprofits only

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pX330A_dCas9-1x3

Plasmid

#63597

Purpose

Expresses dCas9 and gRNA

Depositor

Takashi Yamamoto

Article

Nakagawa et al BMC Biotechnol. 2015 May 22;15(1):33.

Insert

humanized S. pyogenes dCas9

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Promoter

CBh

Available Since

June 1, 2015

Availability

Academic Institutions and Nonprofits only

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pMito-PAGFP-FRB.

Plasmid

#60005

Purpose

Expresses MitoTrap tagged with photo-activatable GFP in mammalian cells.

Depositor

Stephen Royle

Article

Cheeseman et al J Cell Sci. 2013 May 1;126(Pt 9):2102-13

Type

Empty backbone

Use

Knocksideways

Tags

FRB and PAGFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA5 FRT/TO Plk4-mCherry

Plasmid

#80269

Purpose

mammalian expression plasmid for Plk4-mCherry

Depositor

Andrew Holland

Article

Moyer et al J Cell Biol. 2015 Jun 22;209(6):863-78.

Insert

Plk4 (PLK4 Human)

Tags

mCherry

Expression

Mammalian

Available Since

Aug. 10, 2016

Availability

Academic Institutions and Nonprofits only

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pET21b Apaf1 1-591

Plasmid

#26642

Depositor

Guy Salvesen

Article

Pop et al Mol Cell. 2006 Apr 21. 22(2):269-75.

Insert

Apoptotic protease activating factor 1 (APAF1 Human)

Tags

His

Expression

Bacterial

Mutation

Amino Acids 1-591

Available Since

Nov. 10, 2010

Availability

Academic Institutions and Nonprofits only
pUNIV backbone

Plasmid

#24705

Depositor

Cynthia Czajkowski

Article

Venkatachalan et al Pflugers Arch. 2007 Apr . 454(1):155-63.

Type

Empty backbone

Use

Xenopus oocyte expression

Expression

Bacterial, Mammalian, and Y…

Available Since

Dec. 2, 2010

Availability

Academic Institutions and Nonprofits only

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GFP-SENP5

Plasmid

#34555

Depositor

Mary Dasso

Article

Yun et al J Cell Biol. 2008 Nov 17;183(4):589-95.

Insert

SENP5 (SENP5 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 14, 2011

Availability

Academic Institutions and Nonprofits only
pLK65

Plasmid

#37257

Depositor

Ann Hubbard

Article

Kallay et al J Cell Biochem. 2006 Oct 1;99(2):647-64.

Insert

vang-like 2 (VANGL2 Human)

Use

Adenoviral

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 14, 2013

Availability

Academic Institutions and Nonprofits only
pYES2-Ub-R-GFP

Plasmid

#11953

Depositor

Nico Dantuma

Article

Heessen et al FEBS Lett. 2003 Dec 4. 555(2):397-404.

Insert

Ub-R-GFP

Expression

Yeast

Mutation

Ubiquitin fused to N-terminus of GFP. Arginine at…

Available Since

May 17, 2006

Availability

Academic Institutions and Nonprofits only

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pUAS:Cas9T2ACre;U6:sgRNA1;U6:sgRNA2

Plasmid

#74010

Purpose

Tissue-specific knock-out in zebrafish, Cas9 and Cre driven by a UAS promoter

Depositor

Filippo Del Bene

Article

Di Donato et al Genome Res. 2016 Mar 8.

Inserts

5xUAS
Cas9
Cre
U6 promoter

Use

CRISPR

Available Since

April 20, 2016

Availability

Academic Institutions and Nonprofits only

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mCD4 e/p Sal-(hCD2)

Plasmid

#24070

Depositor

Dan Littman

Article

Sawada et al Cell. 1994 Jun 17. 77(6):917-29.

Insert

CD2 (CD2 Human)

Tags

mCD 4 promoter

Expression

Mammalian

Available Since

Oct. 27, 2010

Availability

Academic Institutions and Nonprofits only
pGFP-Rab8A[T22N]

Plasmid

#24899

Depositor

Maxence Nachury

Article

Nachury et al Cell. 2007 Jun 15. 129(6):1201-13.

Insert

hRab8A[T22N]

Tags

GFP

Expression

Mammalian

Mutation

Changed Threonine 22 to Asparagine

Available Since

June 17, 2010

Availability

Academic Institutions and Nonprofits only

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