We narrowed to 4,819 results for: U6

1. pLKO.1-puro-U6-AsCas12a-TLR-MCV1-gRNA

   Plasmid

   #117411

   Purpose

   AsCas12a-gRNA targeting TLR2.0

   Depositor

   Erik Sontheimer

   Article

   Iyer et al CRISPR J. 2022 Oct;5(5):685-701. doi: 10

   Insert

   AsCas12a gRNA targeting TLR 2.0
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Dec. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. AAV-U6-sgRNA-Scaffold-cTNT-SaCas9-HA-OLLAS

   Plasmid

   #209781

   Purpose

   Expresses SaCas9 by the specific cTNT promoter and sgRNA scaffold by U6 promoter

   Depositor

   Yuxuan Guo

   Article

   Yang et al Circulation. 2024 May 28;149(22):1778-17

   Insert

   cTNT
   

   Use

   AAV

   Tags

   HA and OLLAS

   Expression

   Mutation

   Promoter

   cTNT

   Available since

   May 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. AAV-U6-msCAMK2d-sgRNA-cTNT-SaCas9-HA-OLLAS

   Plasmid

   #209782

   Purpose

   Expresses SaCas9 by the specific cTNT promoter and sgRNA targeted the mouse Camk2d gene by U6 promoter

   Depositor

   Yuxuan Guo

   Article

   Yang et al Circulation. 2024 May 28;149(22):1778-17

   Insert

   sgRNA
   

   Use

   AAV

   Tags

   HA and OLLAS

   Expression

   Mutation

   Promoter

   cTNT

   Available since

   May 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLKO.1-puro U6 sgRNA BfuAI stuffer A-tract

   Plasmid

   #138525

   Purpose

   Expresses a guide RNA of choice cloned into the BfuI site extended at the 3' end to incorporate a 220-nt control A-tract repeat RNA sequence.

   Depositor

   Richard Jenner

   Article

   Beltran et al Nat Struct Mol Biol. 2019 Oct;26(10):899

   Insert

   Control non-PRC2 binding A-tract repeat RNA
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Aug. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-RSV-GFP-U6-Rosa26 gRNA (SpyCas9 scaffold)

   Plasmid

   #120296

   Purpose

   AAV vector; encodes GFP as well as a U6-driven Rosa26-targeting gRNA (SpyCas9 scaffold)

   Depositor

   Dominik Niopek

   Article

   Hoffmann et al Nucleic Acids Res. 2019 Apr 15. pii: 545

   Insert

   Rosa26 gRNA (SpCas9 scaffold)
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSLQ2853-5 pHR: U6-Sasgv2CXCR4-3 CMV-EGFP

   Plasmid

   #84256

   Purpose

   Expresses optimized Sa sgCXCR4-3 gRNA with an EGFP fluorescent marker

   Depositor

   Stanley Qi

   Article

   Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

   Insert

   Sa sgCXCR4-3
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   mouse U6

   Available since

   Nov. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSLQ2853-4 pHR: U6-Sasgv2CXCR4-2 CMV-EGFP

   Plasmid

   #84255

   Purpose

   Expresses optimized Sa sgCXCR4-2 gRNA with an EGFP fluorescent marker

   Depositor

   Stanley Qi

   Article

   Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

   Insert

   Sa sgCXCR4-2
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   mouse U6

   Available since

   Nov. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAV-U6+27-Linear-2_PolyU(1)_Tornado-Corn

   Plasmid

   #159487

   Purpose

   Tests for the impact of 1 uracil in a poly-uracil tract on human polymerase III transcription from a U6 promoter.

   Depositor

   Julius Lucks

   Article

   Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

   Insert

   Termination Module:Linear-2_PolyU(1)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6-27

   Available since

   Oct. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pMuLE ENTR U6 stuffer sgRNA scaffold R4-R3

   Plasmid

   #62131

   Purpose

   MuLE (Multiple Lentiviral Expression) Entry vector containing a U6 promoter and sgRNA scaffold module for gRNA expression. Compatible with MultiSite Gateway cloning

   Depositor

   Ian Frew

   Article

   Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

   Type

   Empty backbone

   Use

   CRISPR; Mule gateway entry vector

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. 1531_pAAV-U6-SA-Self1-gRNA-HLP-EmGFP-spA

    Plasmid

    #109318

    Purpose

    Plasmid for liver-specific expression of EmGFP with a gRNA against SaCas9

    Depositor

    William Lagor

    Article

    Li et al Mol Ther Methods Clin Dev. 2018 Dec 6;12

    Insert

    SaCas9 RuvC gRNA
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. p1193-scAAV-U6-sgRNA_Nme2Cas9_Rosa26-CB-PI-AcrIIC3Nme_FLAG_NLS-3XmiR122BS

    Plasmid

    #129531

    Purpose

    Self-complementary AAV vector expressing Nme2Cas9 sgRNA targeting Rosa26 and AcrIIC3Nme with 3x miR-122 binding sites in 3' UTR

    Depositor

    Erik Sontheimer

    Article

    Lee et al RNA. 2019 Aug 22. pii: rna.071704.119. d

    Inserts

    codon-optimized AcrIIC3Nme
    sgRNA_Rosa26
    

    Use

    AAV

    Tags

    FLAG/NLS

    Expression

    Mutation

    Promoter

    U6 and cytomegalovirus-enhancer chicken β-actin p…

    Available since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pMuLE ENTR U6 stuffer sgRNA scaffold L1-L4

    Plasmid

    #62128

    Purpose

    MuLE (Multiple Lentiviral Expression) Entry vector containing a U6 promoter and sgRNA scaffold module for gRNA expression. Compatible with MultiSite Gateway cloning

    Depositor

    Ian Frew

    Article

    Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

    Type

    Empty backbone

    Use

    CRISPR; Mule gateway entry vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. CAGGS-AsCpf1(D908A)-2A-GFP-U6-AAVS1-sgRNA

    Plasmid

    #194727

    Purpose

    CAGGS-AsCpf1(D908A)-2A-GFP-U6-sgRNA-cloning vector, with guide RNA array targeting hAAVS1.

    Depositor

    Rudolf Jaenisch

    Article

    Haiting et al bioRxiv

    Insert

    AsCpf1(D908A) (AAVS1 Acidaminococcus sp.)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pMuLE ENTR U6 stuffer sgRNA scaffold L1-R5

    Plasmid

    #62127

    Purpose

    MuLE (Multiple Lentiviral Expression) Entry vector containing a U6 promoter and sgRNA scaffold module for gRNA expression. Compatible with MultiSite Gateway cloning

    Depositor

    Ian Frew

    Article

    Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

    Type

    Empty backbone

    Use

    CRISPR; Mule gateway entry vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Lenti U6-HBG site1-EF1alpha-UGI-P2A-GFP

    Plasmid

    #157951

    Purpose

    Lentiviral expression of HBG site 1 sgRNA

    Depositor

    Dali Li

    Article

    Zhang et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

    Insert

    Lenti U6-HBG site1-EF1alpha-UGI-P2A-GFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMuLE ENTR U6 stuffer sgRNA scaffold L5-L4

    Plasmid

    #62129

    Purpose

    MuLE (Multiple Lentiviral Expression) Entry vector containing a U6 promoter and sgRNA scaffold module for gRNA expression. Compatible with MultiSite Gateway cloning

    Depositor

    Ian Frew

    Article

    Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

    Type

    Empty backbone

    Use

    CRISPR; Mule gateway entry vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pKLV2-U6(BsmBI)-EF1a-BFP-BSD-Dmap1(SDM)

    Plasmid

    #117139

    Purpose

    Expression vector encoding BFP-BSD-HA-Dmap1 resistant against gRNA encoded by pKLV2-U6(gDmap1 v4)--PGK-Puro-BFP

    Depositor

    Kosuke Yusa

    Article

    Yu et al Stem Cells. 2019 Apr 1. doi: 10.1002/ste

    Insert

    HA-Dmap1 (mutagenised) (Dmap1 Synthetic)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    modified BFP, modified Dmap1 CDS

    Promoter

    human U6 promoter, human EF1a promoter

    Available since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAAV-RSV-GFP-U6-CCR5 gRNA (SpyCas9 scaffold)

    Plasmid

    #113041

    Purpose

    AAV vector; encodes GFP as well as a U6-driven CCR5-targeting gRNA (SpyCas9 scaffold)

    Depositor

    Dominik Niopek

    Article

    Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

    Insert

    gRNA CCR5 (SpCas9 scaffold), co-expressed GFP (transfection marker)
    

    Use

    AAV, CRISPR, and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMuLE ENTR U6 stuffer sgRNA scaffold L5-L2

    Plasmid

    #62130

    Purpose

    MuLE (Multiple Lentiviral Expression) Entry vector containing a U6 promoter and sgRNA scaffold module for gRNA expression. Compatible with MultiSite Gateway cloning

    Depositor

    Ian Frew

    Article

    Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

    Type

    Empty backbone

    Use

    CRISPR; Mule gateway entry vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAV-RSV-GFP-U6-EMX1 gRNA (SpyCas9 scaffold)

    Plasmid

    #113040

    Purpose

    AAV vector; encodes GFP as well as a U6-driven EMX1-targeting gRNA (SpyCas9 scaffold)

    Depositor

    Dominik Niopek

    Article

    Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

    Insert

    gRNA EMX1 (SpCas9 scaffold), co-expressed GFP (transfection marker)
    

    Use

    AAV, CRISPR, and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pFB-U6-Chrna7-gRNA-hSyn-mCherry-WPRE-SV40pA

    Plasmid

    #128342

    Purpose

    pAAV encoding gRNA sequence for loss-of-function indels

    Depositor

    Ryan Drenan

    Article

    Peng et al Eur J Neurosci. 2018 May 19. doi: 10.111

    Insert

    Chrna7 (Chrna7 Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pAAV-RSV-GFP-U6-CFTR gRNA (SpyCas9 scaffold)

    Plasmid

    #113042

    Purpose

    AAV vector; encodes GFP as well as a U6-driven CFTR-targeting gRNA (SpyCas9 scaffold)

    Depositor

    Dominik Niopek

    Article

    Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

    Insert

    CFTR gRNA (SpCas9 scaffold), co-expressed GFP (transfection marker)
    

    Use

    AAV, CRISPR, and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pFB-U6-Chrna3-gRNA-hSyn-mCherry-WPRE-SV40pA

    Plasmid

    #128338

    Purpose

    pAAV encoding gRNA sequence for loss-of-function indels

    Depositor

    Ryan Drenan

    Article

    Peng et al Eur J Neurosci. 2018 May 19. doi: 10.111

    Insert

    Chrna3 (Chrna3 Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pFB-U6-sgNeuN-gRNA-hSyn-mCherry-WPRE-SV40pA

    Plasmid

    #128346

    Purpose

    pAAV encoding control gRNA for CRISPR gRNAs listed above

    Depositor

    Ryan Drenan

    Article

    Peng et al Eur J Neurosci. 2018 May 19. doi: 10.111

    Insert

    Rbfox3 (NeuN) (Rbfox3 Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pFB-U6-Chrna2-gRNA-hSyn-mCherry-WPRE-SV40pA

    Plasmid

    #128337

    Purpose

    pAAV encoding gRNA sequence for loss-of-function indels

    Depositor

    Ryan Drenan

    Article

    Peng et al Eur J Neurosci. 2018 May 19. doi: 10.111

    Insert

    Chrna2 (Chrna2 Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pFB-U6-Chrna4-gRNA-hSyn-mCherry-WPRE-SV40pA

    Plasmid

    #128339

    Purpose

    pAAV encoding gRNA sequence for loss-of-function indels

    Depositor

    Ryan Drenan

    Article

    Peng et al Eur J Neurosci. 2018 May 19. doi: 10.111

    Insert

    Chrna4 (Chrna4 Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pFB-U6-Chrnb3-gRNA-hSyn-mCherry-WPRE-SV40pA

    Plasmid

    #128344

    Purpose

    pAAV encoding gRNA sequence for loss-of-function indels

    Depositor

    Ryan Drenan

    Article

    Peng et al Eur J Neurosci. 2018 May 19. doi: 10.111

    Insert

    Chrnb3 (Chrnb3 Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pAAV-MYL2-HA-TadA8e-Sauri-U6-Camk2d sgRNA7

    Plasmid

    #220127

    Purpose

    Expresses SauriABE8e by MYL2 promoter and sgRNA targeting murine Camk2d intron7 5' splice site by U6 promoter, and add an HA tag fused to TadA

    Depositor

    Yuxuan Guo

    Article

    Liu et al Circ Res. 2025 Jan 16. doi: 10.1161/CIRC

    Insert

    MYL2, TadA, nSauriCas9
    

    Use

    AAV

    Tags

    HA tag

    Expression

    Mutation

    Promoter

    MYL2

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pUC19-U6-SpCas9_gRNA-OPA1-createR194G-A6-gRNA_(CJT88)

    Plasmid

    #226987

    Purpose

    pUC19 plasmid with U6 promoter and SpCas9 gRNA to create an OPA1-R194G cell line via adenine base editing (target base in position A6)

    Depositor

    Benjamin Kleinstiver

    Article

    Fry et al EMBO J. 2024 Feb;43(3):391-413. doi: 10.

    Insert

    SpCas9 gRNA A6 to create OPA1-R194G
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pUC19-U6-SpCas9_gRNA-OPA1-createR194G-A7-gRNA_(CJT89)

    Plasmid

    #226986

    Purpose

    pUC19 plasmid with U6 promoter and SpCas9 gRNA to create an OPA1-R194G cell line via adenine base editing (target base in position A7)

    Depositor

    Benjamin Kleinstiver

    Article

    Fry et al EMBO J. 2024 Feb;43(3):391-413. doi: 10.

    Insert

    SpCas9 gRNA A7 to create OPA1-R194G
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge