Addgene

We narrowed to 6,928 results for: Cad

Showing: 1051 - 1100 of 6928 results

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pJexpress414_OmpA171_H4568

Plasmid

#64296

Purpose

OmpA redesign hybrid with wild-type strands 4568

Depositor

Timothy Whitehead

Article

Stapleton et al Proc Natl Acad Sci U S A. 2015 Jul 21. p

Insert

OmpA_H4568

Use

Synthetic Biology

Tags

6His and FlAsH

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 27, 2015

Availability

Academic Institutions and Nonprofits only
pJexpress414_OmpA171_H1457

Plasmid

#64295

Purpose

OmpA redesign hybrid with wild-type strands 1457

Depositor

Timothy Whitehead

Article

Stapleton et al Proc Natl Acad Sci U S A. 2015 Jul 21. p

Insert

OmpA_H1457

Use

Synthetic Biology

Tags

6His and FlAsH

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 27, 2015

Availability

Academic Institutions and Nonprofits only
pJexpress414_OmpA171_H2467

Plasmid

#64294

Purpose

OmpA redesign hybrid with wild-type strands 2467

Depositor

Timothy Whitehead

Article

Stapleton et al Proc Natl Acad Sci U S A. 2015 Jul 21. p

Insert

OmpA_H2467

Use

Synthetic Biology

Tags

6His and FlAsH

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 27, 2015

Availability

Academic Institutions and Nonprofits only
pJexpress414_OmpA171_H1248

Plasmid

#64293

Purpose

OmpA redesign hybrid with wild-type strands 1248

Depositor

Timothy Whitehead

Article

Stapleton et al Proc Natl Acad Sci U S A. 2015 Jul 21. p

Insert

OmpA_H1248

Use

Synthetic Biology

Tags

6His and FlAsH

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 27, 2015

Availability

Academic Institutions and Nonprofits only
pJexpress414_OmpA171_H2578

Plasmid

#64291

Purpose

OmpA redesign hybrid with wild-type strands 2578

Depositor

Timothy Whitehead

Article

Stapleton et al Proc Natl Acad Sci U S A. 2015 Jul 21. p

Insert

OmpA_H2578

Use

Synthetic Biology

Tags

6His and FlAsH

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 27, 2015

Availability

Academic Institutions and Nonprofits only
pJexpress414_OmpA171_H1678

Plasmid

#62652

Purpose

OmpA redesign hybrid with wild-type strands 1678

Depositor

Timothy Whitehead

Article

Stapleton et al Proc Natl Acad Sci U S A. 2015 Jul 21. p

Insert

OmpA_H1678

Use

Synthetic Biology

Tags

6His and FlAsH

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 27, 2015

Availability

Academic Institutions and Nonprofits only
pJexpress414_OmpA171_H2368

Plasmid

#62651

Purpose

OmpA redesign hybrid with wild-type strands 2368

Depositor

Timothy Whitehead

Article

Stapleton et al Proc Natl Acad Sci U S A. 2015 Jul 21. p

Insert

OmpA_H2368

Use

Synthetic Biology

Tags

6His and FlAsH

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 27, 2015

Availability

Academic Institutions and Nonprofits only
pJexpress414_OmpA171_H3567

Plasmid

#62650

Purpose

OmpA redesign hybrid with wild-type strands 3567

Depositor

Timothy Whitehead

Article

Stapleton et al Proc Natl Acad Sci U S A. 2015 Jul 21. p

Insert

OmpA_H3567

Use

Synthetic Biology

Tags

6His and FlAsH

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 27, 2015

Availability

Academic Institutions and Nonprofits only
pJexpress414_OmpA171_H2345

Plasmid

#62649

Purpose

OmpA redesign hybrid with wild-type strands 2345

Depositor

Timothy Whitehead

Article

Stapleton et al Proc Natl Acad Sci U S A. 2015 Jul 21. p

Insert

OmpA_H2345

Use

Synthetic Biology

Tags

6His and FlAsH

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 27, 2015

Availability

Academic Institutions and Nonprofits only
pJexpress414_OmpA171_H1256

Plasmid

#62648

Purpose

OmpA redesign hybrid with wild-type strands 1256

Depositor

Timothy Whitehead

Article

Stapleton et al Proc Natl Acad Sci U S A. 2015 Jul 21. p

Insert

OmpA_H1256

Use

Synthetic Biology

Tags

6His and FlAsH

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 27, 2015

Availability

Academic Institutions and Nonprofits only
pJexpress414_OmpA171_H1346

Plasmid

#62647

Purpose

OmpA redesign hybrid with wild-type strands 1346

Depositor

Timothy Whitehead

Article

Stapleton et al Proc Natl Acad Sci U S A. 2015 Jul 21. p

Insert

OmpA_H1346

Use

Synthetic Biology

Tags

6His and FlAsH

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 27, 2015

Availability

Academic Institutions and Nonprofits only
pEXP5-NT-AlleyCat

Plasmid

#41715

Depositor

William DeGrado, Ivan Korendovych

Article

Korendovych et al Proc Natl Acad Sci U S A. 2011 Apr 26;10

Insert

mutated C-terminal portion of calmodulin gene (M76-K148)

Use

Tags

Polyhistidine (6×His) region, TEV recognition sit…

Expression

Bacterial

Mutation

F92E

Promoter

T7

Available since

May 8, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
-833pSia-Luc

Plasmid

#32989

Depositor

Sergei Sokol

Article

Fan et al Proc Natl Acad Sci U S A. 1998 May 12;95

Insert

Siamois promoter (sia1.L Frog)

Use

Luciferase

Tags

Luciferase

Expression

Mutation

Promoter

Siamois

Available since

Jan. 5, 2012

Availability

Academic Institutions and Nonprofits only
pRRP51A+intron-LacZ (pHZ18)

Plasmid

#33131

Depositor

Michael Rosbash

Article

Teem et al Proc Natl Acad Sci U S A. 1983 Jul;80(14

Insert

RP51A+intron-LacZ (RPS17A Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Dec. 20, 2011

Availability

Academic Institutions and Nonprofits only
PYBS375

Plasmid

#18887

Depositor

Elaine Elion

Article

Choi et al Cell. 1994 Aug 12. 78(3):499-512.

Insert

Ste11 (STE11 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Lex-Ste11C (amino-terminal 413 amino acids)

Promoter

Available since

July 27, 2008

Availability

Academic Institutions and Nonprofits only
pCAG-Cre

Plasmid

#13775

Purpose

Mammalian expression of Cre recombinase from the broadly active CAG promoter/enhancer

Depositor

Connie Cepko

Article

Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

Insert

Cre

Use

Cre/Lox

Tags

Myc

Expression

Mammalian

Mutation

Promoter

CAG

Available since

April 20, 2007

Availability

Academic Institutions and Nonprofits only

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PPRE X3-TK-luc

Plasmid

#1015

Purpose

PPAR reporter. 3xDR1 sites upstream of a luciferase reporter.

Depositor

Bruce Spiegelman

Article

Kim et al Proc Natl Acad Sci U S A 1998 Apr 14;95(

Insert

PPRE

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 3, 2005

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA HA HP1 alpha

Plasmid

#24078

Depositor

Naoko Tanese

Article

Vassallo et al Proc Natl Acad Sci U S A. 2002 Apr 30. 9

Insert

HP1 alpha (CBX5 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 27, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA HA HP1 gamma

Plasmid

#24080

Depositor

Naoko Tanese

Article

Vassallo et al Proc Natl Acad Sci U S A. 2002 Apr 30. 9

Insert

HP1 gamma (CBX3 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 27, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pMSCV-Zeo

Plasmid

#75088

Purpose

retrovirus-mediated gene transfer into mammalian cells

Depositor

David Mu

Article

Kendall et al Proc Natl Acad Sci U S A. 2007 Oct 16;10

Type

Empty backbone

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 4, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAGIG

Plasmid

#11159

Purpose

Mammalian expression vector for cloning and expressing your gene on a bicistronic mRNA also encoding IRES-GFP

Depositor

Connie Cepko

Article

Matsuda et al Proc Natl Acad Sci U S A. 2004 Jan 6. 10

Has Service

Cloning Grade DNA

Insert

IRES-EGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 13, 2006

Availability

Academic Institutions and Nonprofits only

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pGTR

Plasmid

#63143

Purpose

PCR template to construct polycistronic tRNA-gRNA

Depositor

Yinong Yang

Article

Xie et al Proc Natl Acad Sci U S A. 2015 Mar 17;11

Insert

gRNA-tRNA fusion

Use

CRISPR and Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

April 3, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pRB035

Plasmid

#181944

Purpose

aTc-inducible expression of mNeonGreen

Depositor

Jeffrey Tabor

Article

Butcher et al Proc Natl Acad Sci U S A. 2022 Aug 30;11

Insert

mNeonGreen; tetR (AX04_RS23485 Synthetic)

Use

Tags

Expression

Bacterial

Mutation

Promoter

mNG-PLtetO-1; tetR-J23106

Available since

March 11, 2022

Availability

Academic Institutions and Nonprofits only

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pDF850

Plasmid

#228713

Purpose

Mammalian expression of EVOLVEpro PsaCas12f (epPsaCas12f)

Depositor

Jonathan Gootenberg, Omar Abudayyeh

Article

Jiang et al Science 0, eadr6006

Insert

epPsaCas12f

Use

CRISPR

Tags

Expression

Mammalian

Mutation

I178A/K333V/K454P

Promoter

Available since

Nov. 25, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS2-nCas9n

Plasmid

#47929

Purpose

expression of an optimized Cas9 for genome-editing in zebrafish

Depositor

Wenbiao Chen

Article

Jao et al Proc Natl Acad Sci U S A. 2013 Aug 5.

Insert

nls-zcas9-nls

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_RFP

Plasmid

#206177

Purpose

mammalian expression of RFP

Depositor

Christopher Goodnow

Article

Russell et al Proc Natl Acad Sci U S A. 2021 Aug 24;11

Insert

RFP

Use

Tags

Expression

Mammalian

Mutation

NA

Promoter

CMV

Available since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28:GFP

Plasmid

#60733

Purpose

pET28 driving expression of GFP, biobrick part Bba_E0040

Depositor

Matthew Bennett

Article

Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

Insert

GFP

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 4, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-Cre:GFP

Plasmid

#13776

Purpose

Mammalian expression of Cre recombinase fused to GFP

Depositor

Connie Cepko

Article

Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

Insert

Cre-GFP fusion protein

Use

Cre/Lox

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CAG

Available since

April 20, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-mKelly2

Plasmid

#119783

Purpose

Expresses mKelly2 tagged N-terminally with an MHHHHHHGSG tag

Depositor

Stephen Mayo

Article

Wannier et al Proc Natl Acad Sci U S A. 2018 Nov 27;11

Insert

mKelly2

Use

Tags

6xHis tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 28, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
TRE-mCherry-p2A-eNpHR3.0-TS

Plasmid

#158703

Purpose

Reporter construct

Depositor

Alice Ting

Article

Sanchez et al Proc Natl Acad Sci U S A. 2020 Dec 15. p

Insert

mCherry-p2A-eNpHR3.0-TS

Use

AAV

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

TRE

Available since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEN-luxCDABE

Plasmid

#44918

Depositor

Harry Mobley

Article

Lane et al Proc Natl Acad Sci U S A. 2007 Oct 16;10

Insert

sigma 70 promoter

Use

Luciferase

Tags

lux transcriptional fusion

Expression

Mutation

Promoter

em7

Available since

May 3, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
mPlum-pBAD

Plasmid

#54564

Purpose

Localization: Protein Expression Vector , Excitation: 590, Emission: 649

Depositor

Michael Davidson, Roger Tsien

Article

Wang et al Proc Natl Acad Sci U S A. 2004 Nov 30;10

Type

Empty backbone

Use

Tags

6xHis and mPlum

Expression

Bacterial

Mutation

Promoter

Available since

July 25, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pMXs-Ms-L-Myc

Plasmid

#26023

Depositor

Shinya Yamanaka

Article

Nakagawa et al Proc Natl Acad Sci U S A. 2010 Jul 26. (

Insert

L-Myc (Mycl Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

None

Promoter

Available since

Aug. 20, 2010

Availability

Academic Institutions and Nonprofits only
pVSV Venus VSV-G

Plasmid

#36399

Depositor

Connie Cepko

Article

Beier et al Proc Natl Acad Sci U S A. 2011 Sep 13;10

Insert

VSV Genome

Use

Tags

Expression

Mutation

Promoter

VSV promoters

Available since

March 6, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pVoPo-04

Plasmid

#187839

Purpose

Suicide vector to generate markerless gene deletions in Fusobacterium nucleatum

Depositor

Jörg Vogel

Article

Ponath et al Proc Natl Acad Sci U S A. 2022 Oct 4;119

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 23, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-D12L

Plasmid

#89161

Purpose

Express vaccinia virus D12L in mammalian cells

Depositor

Takeshi Kobayashi

Article

Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. p

Insert

Vaccinia virus D12L

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG promotor

Available since

April 18, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
GST HP1 alpha

Plasmid

#24074

Depositor

Naoko Tanese

Article

Vassallo et al Proc Natl Acad Sci U S A. 2002 Apr 30. 9

Insert

HP1 alpha (CBX5 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 27, 2010

Availability

Academic Institutions and Nonprofits only
pMB80 (R26-CreER)

Plasmid

#12168

Depositor

Tyler Jacks

Article

McLaughlin et al Proc Natl Acad Sci U S A. 2007 Feb 27. 1

Insert

Rosa26 CreER^T2

Use

Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 23, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pBMPC3

Plasmid

#31367

Depositor

Aziz Sancar

Article

Ye et al J Biol Chem. 2011 May 27. ():.

Insert

Bmal1 (Bmal1 Mouse)

Use

Tags

His

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 18, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
Flag-TRIM24

Plasmid

#28138

Depositor

Michelle Barton

Article

Allton et al Proc Natl Acad Sci U S A. 2009 Jul 14. 1

Insert

TRIM24 (TRIM24 Human)

Use

Tags

FLAGX2

Expression

Mammalian

Mutation

Promoter

Available since

June 6, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
[pSK131] pCOLADuet p14|MP1

Plasmid

#136145

Purpose

bacterial expression of p14 and MP1

Depositor

David Sabatini, Kuang Shen

Article

Shen et al Proc Natl Acad Sci U S A. 2018 Sep 18;11

Insert

p14 and MP1

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
GFP-SMN

Plasmid

#37057

Depositor

Greg Matera

Article

Shpargel et al Proc Natl Acad Sci U S A. 2005 Nov 29;10

Insert

Human SMN1 (SMN1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 22, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
co luxE pcDNA3.1(+)

Plasmid

#129089

Purpose

Expression of luxE in mammalian cells

Depositor

Stefan Hell

Article

Gregor et al Proc Natl Acad Sci U S A. 2019 Dec 2. pi

Insert

co luxE

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 8, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
co luxC pcDNA3.1(+)

Plasmid

#129087

Purpose

Expression of luxC in mammalian cells

Depositor

Stefan Hell

Article

Gregor et al Proc Natl Acad Sci U S A. 2019 Dec 2. pi

Insert

co luxC

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 8, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
co luxD pcDNA3.1(+)

Plasmid

#129088

Purpose

Expression of luxD in mammalian cells

Depositor

Stefan Hell

Article

Gregor et al Proc Natl Acad Sci U S A. 2019 Dec 2. pi

Insert

co luxD

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 8, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
co frp pcDNA3.1(+)

Plasmid

#129090

Purpose

Expression of frp in mammalian cells

Depositor

Stefan Hell

Article

Gregor et al Proc Natl Acad Sci U S A. 2019 Dec 2. pi

Insert

co frp

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 8, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
co luxA pcDNA3.1(+)

Plasmid

#129085

Purpose

Expression of luxA in mammalian cells

Depositor

Stefan Hell

Article

Gregor et al Proc Natl Acad Sci U S A. 2019 Dec 2. pi

Insert

co luxA

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 8, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
co luxB pcDNA3.1(+)

Plasmid

#129086

Purpose

Expression of luxB in mammalian cells

Depositor

Stefan Hell

Article

Gregor et al Proc Natl Acad Sci U S A. 2019 Dec 2. pi

Insert

co luxB

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 8, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL2-2xCLEAR-luciferase

Plasmid

#81120

Purpose

Reporter activity of the CLEAR network

Depositor

Sung Hee Baek

Article

Shin et al Nature. 2016 Jun 15. doi: 10.1038/nature

Insert

2xCLEAR

Use

Luciferase

Tags

luciferase

Expression

Mutation

Promoter

2xCLEAR

Available since

Dec. 3, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
EndophilinA1-mRuby2

Plasmid

#171945

Purpose

Expresses Endophilin A1 in mammalian cells

Depositor

Cheng-han Yu

Article

Feng et al Proc Natl Acad Sci U S A. 2021 May 11;11

Insert

Endophilin A1 (SH3GL2 Human)

Use

Tags

mRuby2

Expression

Mammalian

Mutation

Promoter

Available since

July 16, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge

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