We narrowed to 7,696 results for: Pol
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Plasmid#17747DepositorPublicationAvailable sinceMay 14, 2008AvailabilityAcademic Institutions and Nonprofits only
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pTara:500
Plasmid#60717PurposeExpresses T7 RNAP from the arabinose inducible promoter PBAD with a RBS strength of 500 from the Salis RBS calculator. Also includes a constitutive araC ORFDepositorInsertT7 RNAP
UseSynthetic BiologyExpressionBacterialPromoterPBADAvailable sinceNov. 4, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pVITRO1-102.1F10-IgG1/λ
Plasmid#50366PurposeExpresses grass pollen allergen Phl p 7 specific human IgG1/λ antibody isotype (102.1F10-IgG1/λ)DepositorInsertsgrass pollen allergen Phl p 7 specific human Gamma 1 heavy chain expression cassette
grass pollen allergen Phl p 7 specific human lambda light chain expression cassette
ExpressionMammalianPromoterMouse Elongation Factor 1 Alpha and Rat Elongatio…Available sinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3 Plk4(Sak) wt (Nigg HR9)
Plasmid#41165DepositorAvailable sinceJune 7, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pOPINE GFP nanobody:Halo:His6
Plasmid#111090PurposeBacterial expression of a fusion protein consisting of a GFP nanobody (Dr. Brett Collins, Addgene plasmid # 49172), the Halo tag and a His6 tag.DepositorInsertGFP Nanobody
TagsHalo and His6ExpressionBacterial, Insect, and Mamm…PromoterT7 promoterAvailable sinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
FLAG-Pol-II 15
Plasmid#35177DepositorPublicationHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertRPBI (POLR2A Human)
TagsFLAGExpressionMammalianMutationContains the C-terminal repeats 16-52PromoterCMVAvailable sinceMarch 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
FLAG-Pol-II CC
Plasmid#35180DepositorPublicationInsertRPBI (POLR2A Human)
TagsFLAGExpressionMammalianMutationContains C-terminal domain repeats (1-22)X2PromoterCMVAvailable sinceMarch 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
pLV-Ftractin-mCherry
Plasmid#85131Purposelentiviral expression of an F-actin live-cell marker (mCherry)DepositorInsertFtractin-mCherry (amino acids 9-52 of rat ITPKA, C-temrinally tagged with mCherry)
UseLentiviralTagsmCherryPromoterEF1aAvailable sinceFeb. 10, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Flag-HA-PARP11
Plasmid#22556DepositorPublicationAvailable sinceNov. 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
PB-GFP-Gal8
Plasmid#127191PurposePiggybac transposon plasmid with CAG promoter GFP-Galectin 8 (GFP-Gal8) fusion protein. Useful as genetically encoded endosomal escape sensor.DepositorInsertGFP-Gal8 (LGALS8 Human)
TagsGal8 is fused to the c-terminus of GFPExpressionMammalianPromoterCMVAvailable sinceDec. 9, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEGFP-N1 human cofilin S3A
Plasmid#50860Purposeexpresses constituvely active cofilin fused to EGFP in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertcofilin 1 S3A (CFL1 Human)
TagsEGFPExpressionMammalianMutationS3A constitutively activePromoterCMVAvailable sinceFeb. 5, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLV-Golgi eGFP
Plasmid#79809PurposeTo express and target GFP into the Golgi apparatus using lentivirusesDepositorInsertGolgi eGFP (B4GALT1 Human)
UseLentiviralTagsFusion of the first 62 amino acids of the human b…ExpressionMammalianPromoterCMVAvailable sinceJuly 27, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Click editor utilizing mSA for template recruitment and ePhi29 DNA polymerase - pCMV-T7-mSA-nCas9-ePhi29 (JO1398)
Plasmid#217803PurposeVariant CE1 construct with mSA for template recruitment and ePhi29(D169A) DNA pol, expressed from CMV or T7 promoters.DepositorInsertmSA-linker-SV40NLS-nSpCas9-BPNLS-ePhi29-BPNLS
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLSExpressionMammalianMutationnSpCas9(H840A); Phi29(D169A/M8R/V51A/M97T/G197D/E…PromoterCMV and T7Available sinceSept. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDF0112 cmv-cluciferase-polya-ef1a-g-luciferase-polya
Plasmid#186980Purposemammalian expression of Cluc-Gluc reporterDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCluc, Gluc
UseLuciferaseAvailable sinceAug. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEAK-Flag/INPP4B
Plasmid#24324DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertInositol Polyphosphate 4-Phosphatase Type II (INPP4B Human)
TagsFlag and HAExpressionMammalianMutationnoneAvailable sinceMay 11, 2010AvailabilityAcademic Institutions and Nonprofits onlyCited by -
SpyLigase
Plasmid#51722PurposePlasmid for recombinant expression in bacteria of SpyLigase. The Howarth lab has created two improved peptide ligation enzymes. See depositor comments below!DepositorInsertSpyLigase
TagsHis6 TagExpressionBacterialPromoterT7Available sinceMay 1, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
phGluc
Plasmid#22522DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGaussia luciferase
ExpressionMammalianAvailable sinceDec. 7, 2009AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pOPINB-OTULIN (full-length, aa 1-352)
Plasmid#61464PurposeExpresses human OTULIN (full-length) in E. coli.DepositorAvailable sinceMarch 12, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
hGFAP-Cre
Plasmid#40591PurposeMouse astrocyte expression of CreDepositorPublicationInsertCre recombinase (GFAP Human, Mouse, Phage)
UseCre/Lox and Mouse TargetingTagsMP-1 fragment (mouse protamine 1 intron and polya…ExpressionMammalianMutationConstruct contains a nuclear-targeted Cre recombi…PromoterhGFAP promoterAvailable sinceSept. 20, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-UbC
Plasmid#62806PurposeTo express genes at high levels in neuronal cells. This UbC promoter is more active in neurons than the promoter in CMV-based vectors.DepositorTypeEmpty backboneUseAAV; EucaryoticExpressionMammalianPromoterhUbCAvailable sinceMarch 26, 2015AvailabilityAcademic Institutions and Nonprofits only