We narrowed to 14,149 results for: nts

1. pNTI820

   Plasmid

   #216629

   Purpose

   cpTurboID (V5) for mammalian expression and lentivirus production

   Depositor

   Nicholas Ingolia

   Article

   Kim et al Nat Chem Biol. 2024 Jul;20(7):894-905. d

   Insert

   cpTurboID
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pNTI819

   Plasmid

   #216628

   Purpose

   Cal-ID (V5) for mammalian expression and lentivirus production

   Depositor

   Nicholas Ingolia

   Article

   Kim et al Nat Chem Biol. 2024 Jul;20(7):894-905. d

   Insert

   Cal-ID
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pScPPX2

   Plasmid

   #112877

   Purpose

   Expresses His-tagged yeast exopolyphosphatase for purification from E. coli

   Depositor

   Michael Gray, Ursula Jakob

   Article

   Gray et al Mol Cell. 2014 Mar 6;53(5):689-99. doi:

   Insert

   PPX1 (PPX1 Budding Yeast)
   

   Use

   Tags

   6X His tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pNL4-3 envFS eGFP gag2xFS

   Plasmid

   #101341

   Purpose

   1st frameshift is CG nucleotide insertion in MA at nt 832. 2nd is a CTAG addition in CA at nt 1508.

   Depositor

   Jeremy Luban

   Article

   McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

   Insert

   NL4-3
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pNP561

   Plasmid

   #222723

   Purpose

   Target and Recombinase Golden Gate Landing Pad Backbone for LT-RT with T7 Recombinase

   Depositor

   Patrick Hsu

   Article

   Durrant et al Nature. 2024 Jun;630(8018):984-993. doi:

   Type

   Empty backbone

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pNP555

   Plasmid

   #222722

   Purpose

   sfGFP-ON BsaI Golden Gate Landing Pad Backbone for RE-LE (bRNA in cis expression)

   Depositor

   Patrick Hsu

   Article

   Durrant et al Nature. 2024 Jun;630(8018):984-993. doi:

   Type

   Empty backbone

   Use

   Tags

   sfGFP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pNTI757 P(PGK1)-citrine P(HIS4)-mCherry

   Plasmid

   #164917

   Purpose

   Divergent promoter expression cassette, P(HIS4) expression with P(PGK1) normalizer

   Depositor

   Nicholas Ingolia

   Article

   Muller et al Science. 2020 Dec 11;370(6522). pii: 370

   Insert

   P(PGK1)-citrine and P(HIS4)-mCherry
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   PGK1 (yeast) and HIS4 (yeast)

   Available since

   Feb. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pYO831

   Plasmid

   #235754

   Purpose

   Protein expression of ZZ-ScVPS38 (1-212F)

   Depositor

   Roger Williams

   Article

   Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

   Insert

   VPS38
   

   Use

   Tags

   ZZ-3xTEV

   Expression

   Yeast

   Mutation

   aa 1-212F

   Promoter

   Available since

   May 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pYO225

   Plasmid

   #235729

   Purpose

   Protin expression of FL yeast VPS15 -ZZ in yeast

   Depositor

   Roger Williams

   Article

   Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

   Insert

   VPS15
   

   Use

   Tags

   3xTEV-ZZ

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pYO290

    Plasmid

    #235731

    Purpose

    Protein expression in FL yeast VPS34 D731N mutant in yeast

    Depositor

    Roger Williams

    Article

    Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

    Insert

    VPS34
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    S2A, D731N

    Promoter

    Available since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pYO478

    Plasmid

    #235757

    Purpose

    Expression of FL ScVPS34-3xFLAG under its own promoter

    Depositor

    Roger Williams

    Article

    Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

    Insert

    VPS34
    

    Use

    Tags

    3xFLAG

    Expression

    Yeast

    Mutation

    Promoter

    ScVPS34 promoter

    Available since

    May 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pYO764

    Plasmid

    #235761

    Purpose

    Expression of ScVPS38-EGFP under its own promoter

    Depositor

    Roger Williams

    Article

    Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

    Insert

    VPS38
    

    Use

    Tags

    EGFP

    Expression

    Yeast

    Mutation

    Promoter

    ScVPS38 promoter

    Available since

    May 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pYO932

    Plasmid

    #235764

    Purpose

    Expression of ScVPS34 HELCAT (268-875)-3xFLAG under its own promoter

    Depositor

    Roger Williams

    Article

    Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

    Insert

    VPS34 HELCAT
    

    Use

    Tags

    3xFLAG

    Expression

    Yeast

    Mutation

    aa 268-875

    Promoter

    ScVPS34 promoter

    Available since

    May 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pNTI763 p(Z)-mCherry + UBC9 sgRNA

    Plasmid

    #164918

    Purpose

    CEN/ARS with P(Z)-mCherry controlled by the ZEM transcription factor, also encode UBC9 sgRNA

    Depositor

    Nicholas Ingolia

    Article

    Muller et al Science. 2020 Dec 11;370(6522). pii: 370

    Insert

    P(Z)-mcherry
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    P(Z), synthetic promoter

    Available since

    Feb. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pNTI751 pPGK1-GCN4(UTR)-ZEM(TF)

    Plasmid

    #164916

    Purpose

    Expresses the ZEM transcription factor under the translational control of the yeast GCN4 5'UTR

    Depositor

    Nicholas Ingolia

    Article

    Muller et al Science. 2020 Dec 11;370(6522). pii: 370

    Insert

    GCN4(UTR)-ZEM(TF)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    PGK1 (yeast)

    Available since

    Feb. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRPL28exon1-(Nhe/Mfe) SpHIS5 (pNTI619)

    Plasmid

    #115432

    Purpose

    Vector for expressing RPL28 with mutagenized exon2

    Depositor

    Nicholas Ingolia

    Article

    McGeachy et al ACS Synth Biol. 2019 Apr 19;8(4):844-856

    Inserts

    RPL28 (RPL28 Budding Yeast)
    HIS5
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    exon 2 deleted, with MfeI / NheI cloning site

    Promoter

    Ashbya gospii TEF1 and endogenous

    Available since

    Oct. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. 4G-Cloning Plasmid Kit

    Plasmid Kit

    #1000000252

    Purpose

    The 4G (Golden-Gate-guided Gibson) cloning kit provides elements for rapid one-day assembly of multi-gene expression vectors in bacterial and eukaryotic expression hosts.

    Depositor

    Stephan Gruber

    Available since

    Jan. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

18. pICH41331::pU6cm-pegR::EURb7

    Plasmid

    #227695

    Purpose

    Golden Gate level 0 acceptor vector for cloning altered epegRNA: altered epegRNA being driven by U6 composite promoter (pU6cm) and terminated by a dual terminator (EURb7).

    Depositor

    Jae-Yean Kim

    Article

    Vu et al Nat Plants. 2024 Sep 6. doi: 10.1038/s41

    Insert

    Altered epegRNA backbone with cloning sites for gRNA and RTT.
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    U6 composite

    Available since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pICH47742::p35S-PE2max-NC::tEURb7

    Plasmid

    #227693

    Purpose

    Golden Gate level 1, position 2, PE2max-NC expression cassette.

    Depositor

    Jae-Yean Kim

    Article

    Vu et al Nat Plants. 2024 Sep 6. doi: 10.1038/s41

    Insert

    PE2max-NC being driven by long CaMV 35S (p35S) and terminated by a dual terminator (EURb7).
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    CaMV 35S

    Available since

    Nov. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pICH47742:: pRPS5a-PE2max-NC::tHSP

    Plasmid

    #227694

    Purpose

    Golden Gate level 1, position 2, PE2max-NC expression cassette for Arabidopsis.

    Depositor

    Jae-Yean Kim

    Article

    Vu et al Nat Plants. 2024 Sep 6. doi: 10.1038/s41

    Insert

    PE2max-NC being driven by pRPS5a and terminated by tHSP
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    pRPS5a

    Available since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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