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Plasmid#97086PurposeEncodes an sgRNA that creates a SSB near the poly(A) site of human NEAT1_1 isoform.DepositorInsertsgRNA NEAT1_IS_v2
UseCRISPRTagsExpressionMutationPromoterU6Available sinceJuly 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSB_init-Sb44
Plasmid#159421PurposeExpression of the anti-TGP (thermostable GFP) synthetic nanobody Sb44DepositorInsertSb44
UseTagsc-Myc and poly-HisExpressionBacterialMutationPromoterAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLV.PARP1#5
Plasmid#14548DepositorInsertPARP1 shRNA (PARP1 Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceMarch 27, 2007AvailabilityAcademic Institutions and Nonprofits only -
pLV.PARP1#4
Plasmid#14547DepositorInsertPARP1 shRNA (PARP1 Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceMarch 27, 2007AvailabilityAcademic Institutions and Nonprofits only -
pSB_init_Sb68
Plasmid#159423PurposeExpression of the anti-TGP (thermostable GFP) synthetic nanobody Sb68DepositorInsertSb68
UseTagsc-Myc and poly-HisExpressionBacterialMutationPromoterAvailable sinceJan. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pT3TS-HiFi-nCas9n-A72
Plasmid#125646Purposemicro-injection of poly-adenylated HiFi-nCas9n RNADepositorInsertHiFi-nCas9n
UseRna in vitro transcription vectorTagsExpressionMutationR691A from wild-type Cas9PromoterT3Available sinceJuly 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSB_init_Sb92
Plasmid#159424PurposeExpression of the anti-TGP (thermostable GFP) synthetic nanobody Sb92DepositorInsertSb92
UseTagsc-Myc and poly-HisExpressionBacterialMutationPromoterAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYTSG
Plasmid#159419PurposeExpression of proteins with a C-terminal thermostable GFP (TGP) tag in Saccharomyces cerevisiaeDepositorInsertthermostable GFP (TGP)
UseTagspolyhistidine (poly-His)ExpressionYeastMutationPromoterAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLV.PARP1#3
Plasmid#14546DepositorInsertPARP1 shRNA (PARP1 Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceMarch 27, 2007AvailabilityAcademic Institutions and Nonprofits only -
pSB_init-Sb66
Plasmid#159422PurposeExpression of the anti-TGP (thermostable GFP) synthetic nanobody Sb66DepositorInsertSb66
UseTagsc-Myc and poly-HisExpressionBacterialMutationPromoterAvailable sinceApril 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
Pub-mCD8:GFP-T2A-DsRed:NLS-SV40
Plasmid#130665PurposePlasmid to test the efficiency of T2A multiplexing in Aedes aegyptiDepositorInsertAaegPUb-mCD8-GFP-T2A-dsRed-NLS-SV40
UseAe. aegypti expressionTagsExpressionInsectMutationPromoterAedes aegypti poly-ubiquitinAvailable sinceOct. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-SCP1-dSa VPR mini.-2X snRP-1 Afp
Plasmid#99696PurposeExpresses dSa Cas9 fused to optimized combination of truncated activation domains from SCP1 promoter with dual snRP1 poly adenylation signal, and Sa sgRNA for Afp, vector allows for strong activation of mouse Afp, can be packaged and delivered as AAVDepositorInsertdCas9 and gRNA targeting Afp (Afp Synthetic)
UseAAVTagsVPR miniExpressionMutationdead Cas9PromoterAvailable sinceNov. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHIT239
Plasmid#204522PurposeIn vitro transcription of Mos1 transposase mRNA with SL1 and poly(A) used for microinjection into nematodeDepositorInsertMos1_transposase::glh-2_3'UTR (glh-2 Nematode)
UseOtherTagsExpressionMutationPromoterT7 (minimum seq.)Available sinceAug. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJEP206-AAV-1.3CaMKII-MCS-pA.
Plasmid#78319PurposeAAV vector backbone containing a 1.3CaMKIIa promoter followed by a multiple cloning site(MCS) followed by a poly-Adenylation Signal (pA)DepositorTypeEmpty backboneUseAAVTagsExpressionMutationPromoter1.3CaMKIIaAvailable sinceMarch 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJEP210-AAV-0.4CaMKII P-MCS-pA
Plasmid#63084PurposeAAV2 vector backbone containing a 0.4CaMKIIa promoter followed by a multiple cloning site(MCS) followed by a poly-Adenylation Signal (pA)DepositorTypeEmpty backboneUseAAVTagsExpressionMutationPromoter0.4αCaMKIIAvailable sinceMarch 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJEP222-AAV-CMV-GFP-pA
Plasmid#62916PurposeAAV vector backbone designed to express GFP from a CMV promoter. It also contains a poly-Adenylation Signal (pA)DepositorInsertGreen Fluorescent protein (GFP)
UseAAVTagsExpressionMutationPromoterCMVAvailable sinceMarch 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJEP220-AAV-0.5Synapsin-GFP-pA
Plasmid#62920PurposeAAV vector backbone designed to express GFP from a 0.5Synapsin promoter. It also contains a poly-Adenylation Signal (pA)DepositorInsertGreen Fluorescent protein (GFP)
UseAAVTagsExpressionMutationPromoter0.5synapsinAvailable sinceMarch 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJEP219-AAV-1.1Synapsin-GFP-pA
Plasmid#62921PurposeAAV vector backbone designed to express GFP from a 1.1Synapsin promoter. It also contains a poly-Adenylation Signal (pA)DepositorInsertGreen Fluorescent protein (GFP)
UseAAVTagsExpressionMutationPromoter1.1SynapsinAvailable sinceMarch 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCRII-TOPO CMV-cGFP-SV40 pA + Laccase2 Exon 2
Plasmid#91802PurposeExpresses the Laccase2 circular RNA when the upstream SV40 poly(A) signal fails to be usedDepositorInsertCoral Green Fluorescent Protein (cGFP)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
c-Flag pcDNA3
Plasmid#20011PurposeThis pcDNA3 plasmid contains a new poly-linker and single C-terminal flag-tag sequence for expression in mammalian cells.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseTagsFlagExpressionMammalianMutationPromoterAvailable sinceJan. 28, 2009AvailabilityAcademic Institutions and Nonprofits only