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pRK5-HA-Ubiquitin-K33

Plasmid

#17607

Purpose

Mammalian expression of HA tagged ubiquitin with K33 only, other lysines mutated to arginines

Depositor

Ted Dawson

Publication

Lim et al J Neurosci. 2005 Feb 23. 25(8):2002-9.

Insert

Ubiquitin C (UBC Human)

Tags

HA

Expression

Mammalian

Mutation

K33 only, other lysines mutated to arginines; P19…

Available since

March 31, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

30 Articles

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GW1CMV-Perceval

Plasmid

#21737

Purpose

can be used to monitor the ATP:ADP ratio during live-cell imaging

Depositor

Gary Yellen

Publication

Berg et al Nat Methods. 2009 Feb;6(2):161-6. doi: 1

Insert

Perceval (fluorescent ATP:ADP sensor)

Tags

5'UTR

Expression

Mammalian

Mutation

improved Kozak sequence

Available since

Aug. 5, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pMXs-Nanog

Plasmid

#13354

Purpose

Retroviral expression of mouse Nanog

Depositor

Shinya Yamanaka

Publication

Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

Insert

Nanog homeobox (Nanog Mouse)

Use

Retroviral

Expression

Mammalian

Mutation

None

Available since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pcDNA3.1(+)IRES GFP

Plasmid

#51406

Purpose

Expresses GFP from internal ribosome entry sequence, cloning component

Depositor

Kathleen_L Collins

Publication

Schaefer et al J Immunol. 2008 Jun 15;180(12):7804-17.

Insert

IRES GFP

Expression

Mammalian

Promoter

CMV

Available since

May 21, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

27 Articles

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IKK-2 S177E S181E

Plasmid

#11105

Purpose

Mammalian expression of constitutively active human IKK-2

Depositor

Anjana Rao

Publication

Mercurio et al Science. 1997 Oct 31. 278(5339):860-6.

Insert

IKK-2 (IKBKB Human)

Tags

FLAG

Expression

Mammalian

Mutation

S177E and S181E, constitutively active

Available since

Feb. 14, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

34 Articles

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pcDNA3-HA-Sumo1

Plasmid

#21154

Purpose

Mammalian expression of human Sumo1 with HA tag

Depositor

Junying Yuan

Publication

Terui et al J Biol Chem. 2004 Jul 2. 279(27):28257-6

Insert

pcDNA3-HA-Sumo1 (SUMO1 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available since

May 11, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

15 Articles

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mVenus C1

Plasmid

#27794

Depositor

Steven Vogel

Publication

Koushik et al Biophys J. 2006 Dec 15. 91(12):L99-L101.

Insert

mVenus C1

Expression

Mammalian

Available since

April 19, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

31 Articles

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pMKO.1 GFP

Plasmid

#10676

Purpose

Retroviral shRNA vector. Derivative of pMKO.1 puro (Addgene plasmid #8452) with GFP instead of puromycin resistance gene.

Depositor

William Hahn

Type

Empty backbone

Use

RNAi and Retroviral

Expression

Mammalian

Available since

Dec. 7, 2005

Availability

Academic Institutions and Nonprofits only

Cited by

26 Articles

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Lact-C2-GFP

Plasmid

#22852

Depositor

Sergio Grinstein

Publication

Yeung et al Science. 2008 Jan 11. 319(5860):210-3.

Insert

Lactadherin-C2 Domain (MFGE8 Bovine)

Tags

GFP

Expression

Mammalian

Available since

Jan. 15, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

39 Articles

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pENTR1A no ccDB (w48-1)

Plasmid

#17398

Purpose

Gateway entry 1A vector, no ccDB gene

Depositor

Eric Campeau, Paul Kaufman

Publication

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Gateway Cloning

Expression

Mammalian

Available since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

78 Articles

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XLone-GFP

Plasmid

#96930

Purpose

Tunable and temporal expression control of GFP

Depositor

Xiaojun Lian

Publication

Randolph et al Sci Rep. 2017 May 8;7(1):1549. doi: 10.1

Insert

Green Fluorescent Protein

Expression

Mammalian

Promoter

TRE3GS

Available since

Aug. 3, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

38 Articles

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pIRESneo-FLAG/HA Ago2 corrected

Plasmid

#10822

Depositor

Thomas Tuschl

Publication

Meister et al Mol Cell. 2004 Jul 23. 15(2):185-97.

Insert

Argonaute 2 (AGO2 Human)

Tags

FLAG and HA

Expression

Mammalian

Mutation

3 silent mutations in orf

Available since

Nov. 2, 2005

Availability

Academic Institutions and Nonprofits only

Cited by

52 Articles

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NFAT luciferase reporter

Plasmid

#10959

Depositor

Toren Finkel

Publication

Ichida et al J Biol Chem. 2001 Feb 2. 276(5):3524-30.

Insert

IL-2 promoter (IL2 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Available since

Jan. 6, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

23 Articles

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HA-HIF2alpha-pcDNA3

Plasmid

#18950

Depositor

William Kaelin

Publication

Kondo et al Cancer Cell. 2002 Apr . 1(3):237-46.

Insert

Hypoxia inducible factor 2 alpha (EPAS1 Human)

Tags

HA-tag

Expression

Mammalian

Mutation

wt cDNA

Available since

Aug. 20, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

27 Articles

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pAC154-dual-dCas9VP160-sgExpression

Plasmid

#48240

Purpose

Dual expression construct expressing both dCas9VP160 and sgRNA from separate promoters

Depositor

Rudolf Jaenisch

Publication

Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

Insert

dCas9

Use

CRISPR

Tags

HA-Tag, HA-tag, and VP160

Expression

Mammalian

Mutation

D10A H840A

Available since

Sept. 27, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

24 Articles

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p1321 HPV-16 E6/E7

Plasmid

#8641

Depositor

Peter Howley

Publication

Münger et al J Virol. 1989 Oct . 63(10):4417-21.

Insert

HPV-16 E6/E7 (E7, E6 Human papillomavirus)

Expression

Mammalian

Available since

June 16, 2005

Availability

Academic Institutions and Nonprofits only

Cited by

16 Articles

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pSpCas9 (PX165)

Plasmid

#48137

Purpose

Human codon optimized Cas9 nuclease from Streptococcus pyogenes (Cbh-3X-FLAG-NLS-SpCas9-NLS)

Depositor

Feng Zhang

Publication

Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

Insert

hSpCas9

Use

CRISPR

Tags

3XFLAG

Expression

Mammalian

Promoter

Cbh

Available since

Oct. 29, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

35 Articles

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Tet-pLKO-puro-Scrambled

Plasmid

#47541

Purpose

Tet inducible scrambled shRNA.

Depositor

Charles Rudin

Publication

Rudin et al Nat Genet. 2012 Oct;44(10):1111-6. doi:

Insert

Scrambled shRNA

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

H1

Available since

April 28, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

23 Articles

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GFP-Nucleolin

Plasmid

#28176

Depositor

Michael Kastan

Publication

Takagi et al Cell. 2005 Oct 7. 123(1):49-63.

Insert

Nucleolin (NCL Human)

Tags

GFP

Expression

Mammalian

Available since

Feb. 24, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

22 Articles

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pcDNA3 mCherry LIC cloning vector (6B)

Plasmid

#30125

Purpose

Empty LIC vector; adds an mCherry gene to the C-terminus of your protein of interest.

Depositor

Scott Gradia

Type

Empty backbone

Tags

mCherry

Expression

Mammalian

Available since

June 9, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

23 Articles

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