We narrowed to 59,444 results for: cloning

1. p663-UBC-miniTurbo-V5-NIM1K_IDG-K

   Plasmid

   #189892

   Purpose

   Gateway destination clone of NIM1K (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

   Depositor

   Ben Major

   Insert

   NIM1K (NIM1 Human)
   

   Use

   Lentiviral; Gateway destination

   Tags

   miniTurbo-V5

   Expression

   Mammalian

   Promoter

   Ubiquitin

   Available Since

   Oct. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFH17

   Plasmid

   #125803

   Purpose

   Level0 LbCas12a, rice codon optimized

   Depositor

   Vladimir Nekrasov

   Article

   Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

   Insert

   LbCas12a, rice codon optimized
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Plant

   Available Since

   Aug. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. mChT pcDNA3

   Plasmid

   #15765

   Depositor

   Randy Blakely

   Article

   Apparsundaram et al Biochem Soc Trans. 2001 Nov . 29(Pt 6):7

   Insert

   hemicholinium-3-sensitive choline transporter (Slc5a7 Mouse)
   

   Expression

   Mammalian

   Available Since

   Sept. 13, 2007

   Availability

   Academic Institutions and Nonprofits only

4. FEM1C

   Plasmid

   #167842

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   FEM1C (FEM1C Human)
   

   Tags

   6xHis-TEV

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   April 22, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pJMP2849

   Plasmid

   #160677

   Purpose

   Mobile CRISPRi empty sgRNA (BsaI) for cloning new sgRNAs

   Depositor

   Jason Peters

   Article

   Banta et al Curr Protoc Microbiol. 2020 Dec;59(1):e1

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   Jan. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. L3MBTL2

   Plasmid

   #25244

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   L3MBTL2_14 (L3MBTL2 Human)
   

   Tags

   His

   Expression

   Bacterial

   Available Since

   Aug. 13, 2010

   Availability

   Industry, Academic Institutions, and Nonprofits

7. p663-UBC-miniTurbo-V5-PIP5K1B_IDG-K

   Plasmid

   #170599

   Purpose

   Gateway destination clone of PIP5K1B (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

   Depositor

   Ben Major

   Insert

   miniTurbo-V5-PIP5K1B (PIP5K1B Human)
   

   Use

   Lentiviral; Gateway destination

   Tags

   miniTurbo-V5

   Expression

   Mammalian

   Promoter

   Ubiquitin

   Available Since

   Sept. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. p667-UBC-TLK2-V5_IDG-K

   Plasmid

   #135251

   Purpose

   Gateway destination clone of TLK2 (human) tagged with C-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

   Depositor

   Ben Major

   Insert

   TLK2-V5 (TLK2 Human)
   

   Use

   Lentiviral; Gateway destination

   Tags

   V5

   Expression

   Mammalian

   Promoter

   Ubiquitin

   Available Since

   Jan. 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. DLC1A

   Plasmid

   #25333

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   DLC1:S1074-L1283 (DLC1 Human)
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   Contains amino acids S1074-L1283

   Available Since

   Aug. 17, 2010

   Availability

   Industry, Academic Institutions, and Nonprofits

10. prGT3

    Plasmid

    #15993

    Depositor

    Morris Birnbaum

    Article

    Birnbaum et al Proc Natl Acad Sci U S A. 1986 Aug . 83(

    Insert

    GLUT1 (Slc2a1 Rat)
    

    Expression

    Bacterial

    Available Since

    Oct. 26, 2007

    Availability

    Academic Institutions and Nonprofits only

11. pFH22

    Plasmid

    #125808

    Purpose

    Level0 xCas9 3.7

    Depositor

    Vladimir Nekrasov

    Article

    Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

    Insert

    xCas9 3.7
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Plant

    Available Since

    Aug. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. HOXA2 (murine) HIS-tag pET

    Plasmid

    #8557

    Depositor

    Corey Largman

    Insert

    HOXA2 (Hoxa2 Mouse)
    

    Tags

    His and T7

    Expression

    Bacterial

    Mutation

    contains 5 additional amino acids from 5' un…

    Available Since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only

13. pDD372

    Plasmid

    #91824

    Purpose

    Codon-optimized GFP donor for the SapTrap cloning system

    Depositor

    Bob Goldstein

    Article

    Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

    Insert

    GFP-C1
    

    Use

    CRISPR

    Expression

    Worm

    Available Since

    June 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRMA154

    Plasmid

    #167554

    Purpose

    S. flexneri O antigen genes cloned from pPM2213 into pJRD215

    Depositor

    Renato Morona

    Article

    Morona et al J Bacteriol. 1994 Feb;176(3):733-47. doi

    Insert

    S. flexneri O antigen genes cloned from pPM2213 (ClaI - KpnI)
    

    Use

    Broad host range mobilisable

    Available Since

    May 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. P-BT1763

    Plasmid

    #188111

    Purpose

    pBolux harboring the promoter region upstream of BT1763

    Depositor

    Guy Townsend

    Article

    Modesto et al Nat Commun. 2023 Jan 17;14(1):275. doi:

    Insert

    The B. thetaiotaomicron BT1763 promoter cloned into pBolux
    

    Expression

    Bacterial

    Promoter

    BT1763

    Available Since

    Jan. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDD377

    Plasmid

    #91823

    Purpose

    Codon-optimized mTurquoise2 donor for the SapTrap cloning system

    Depositor

    Bob Goldstein

    Article

    Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

    Insert

    mTurquoise2-C1
    

    Use

    CRISPR

    Expression

    Worm

    Available Since

    June 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pFH56

    Plasmid

    #125882

    Purpose

    Level0 Rice OsACT1 Pol II promoter

    Depositor

    Vladimir Nekrasov

    Article

    Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

    Insert

    Level0 Rice OsAct1 Pol II promoter
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Plant

    Available Since

    Aug. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. p663-UBC-miniTurbo-V5-RIOK2_IDG-K

    Plasmid

    #170586

    Purpose

    Gateway destination clone of RIOK2 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    miniTurbo-V5-RIOK2 (RIOK2 Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    miniTurbo-V5

    Expression

    Mammalian

    Promoter

    Ubiquitin

    Available Since

    Sept. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. p663-UBC-miniTurbo-V5-STK32A_IDG-K

    Plasmid

    #170608

    Purpose

    Gateway destination clone of STK32A (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    miniTurbo-V5-STK32A (STK32A Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    miniTurbo-V5

    Expression

    Mammalian

    Promoter

    Ubiquitin

    Available Since

    June 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. p667-UBC-PKMYT1-V5-TurboID_IDG-K

    Plasmid

    #135247

    Purpose

    Gateway destination clone of PKMYT1 (human) tagged with C-terminal V5-TurboID for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    PKMYT1-V5-TurboID (PKMYT1 Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    V5-TurboID

    Expression

    Mammalian

    Promoter

    Ubiquitin

    Available Since

    Sept. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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