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pB-CAG-FLEX-F14F15-BFP-(mCherry)'

Plasmid

#201831

Purpose

piggyBac transposon plasmid for making Cre and Flpo reporter cell lines using piggyBac transposase

Depositor

Ian Wickersham

Article

Jin et al Cell Rep Methods. 2023 Nov 20;3(11):1006

Inserts

CAG
BFP

Expression

Mammalian

Promoter

CAG

Available Since

April 5, 2024

Availability

Academic Institutions and Nonprofits only

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pQCXIZ GFP C1

Plasmid

#202424

Purpose

Encodes GFP alone

Depositor

David Bryant

Article

Roman-Fernandez et al Sci Adv. 2023 Feb 3;9(5):eabq1858. doi:

Type

Empty backbone

Use

Lentiviral

Available Since

Aug. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pORANGE GFP-Bsn KI

Plasmid

#139664

Purpose

Endogenous tagging of Bassoon: N-terminal (amino acid position: L7)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor

Expression

Mammalian

Promoter

U6 and Cbh

Available Since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

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pENTR223-PLXNB2

Plasmid

#86236

Purpose

PLXNB2 full-length human cDNA CDS in gateway entry plasmid

Depositor

Roland Friedel

Article

Huang et al Commun Biol. 2021 Jan 29;4(1):145. doi:

Insert

PLXNB2 (PLXNB2 Human)

Use

Gateway entry vector

Available Since

Jan. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-eGFP-P2A-LgBiT-NLS

Plasmid

#187834

Purpose

Expression of eGFP-P2A-LgBiT-NLS from human synapsin promoter via adeno-associated virus for neuronal expression.

Depositor

William McEwan

Article

Tuck et al Cell Rep. 2022 May 3;39(5):110776. doi:

Insert

eGFP-P2A-LgBiT-NLS

Use

AAV

Promoter

hSyn

Available Since

Sept. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pORANGE GFP-Pclo KI

Plasmid

#139657

Purpose

Endogenous tagging of Piccolo: N-terminal (amino acid position: G2)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor

Expression

Mammalian

Promoter

U6 and Cbh

Available Since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

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pMXs-IP-EGFP-LC3

Plasmid

#38195

Depositor

Noboru Mizushima

Article

Hara et al J Cell Biol. 2008 May 5. 181(3):497-510.

Insert

microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Available Since

Aug. 16, 2012

Availability

Academic Institutions and Nonprofits only

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pMXs-IP-EGFP-mAtg5

Plasmid

#38196

Depositor

Noboru Mizushima

Article

Hara et al J Cell Biol. 2008 May 5. 181(3):497-510.

Insert

autophagy related genes 5 (Atg5 Mouse)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Available Since

Aug. 16, 2012

Availability

Academic Institutions and Nonprofits only

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pEF1α:flex-mcherry

Plasmid

#222618

Purpose

Cre-dependent expression of the red fluorescent protein mCherry under control of the EF1a promoter

Depositor

Franck Polleux

Article

Blockus et al Cell Rep. 2021 Oct 19;37(3):109828. doi:

Insert

Cre-dependent mCherry

Use

AAV

Available Since

July 9, 2024

Availability

Academic Institutions and Nonprofits only

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pMXs-Lyl1

Plasmid

#131612

Purpose

expression of mouse LyI1 in mammalian cells

Depositor

Kateri Moore, Filipe Pereira

Article

Pereira et al Cell Stem Cell. 2013 Aug 1;13(2):205-18.

Insert

Lyl1 (Lyl1 Mouse)

Use

Retroviral

Expression

Mammalian

Available Since

Dec. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pORANGE Rims1-GFP KI

Plasmid

#131494

Purpose

Endogenous tagging of RIM1: C-terminal (amino acid position: S1615)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor (Rims1 Rat)

Expression

Mammalian

Promoter

U6 and Cbh

Available Since

Sept. 25, 2019

Availability

Academic Institutions and Nonprofits only

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ERG_pCSdest

Plasmid

#53864

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

ERG (ERG Human)

Expression

Mammalian

Promoter

SP6 and CMV

Available Since

March 27, 2018

Availability

Industry, Academic Institutions, and Nonprofits
pB-Ric8b-Integration

Plasmid

#129457

Purpose

piggyBac transposon vector that Inducibly (Tet-On) expresses Ric8b

Depositor

Sriram Kosuri

Article

Jones et al Cell Syst. 2019 Mar 27;8(3):254-260.e6.

Insert

Ric8b (Ric8b Mouse)

Use

Synthetic Biology

Expression

Mammalian

Promoter

TRE (Tet-On)

Available Since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pMXs-Tcf3

Plasmid

#131598

Purpose

expression of mouse Tcf3 in mammalian cells

Depositor

Kateri Moore, Filipe Pereira

Article

Pereira et al Cell Stem Cell. 2013 Aug 1;13(2):205-18.

Insert

Tcf3 (Tcf3 Mouse)

Use

Retroviral

Expression

Mammalian

Available Since

Dec. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti Dest PuroR R1-R2

Plasmid

#84575

Purpose

Gateway destination vector expressing Puromycin resistance under a PGK promoter

Depositor

Ghassan Mouneimne

Article

Padilla-Rodriguez et al Nat Commun. 2018 Jul 30;9(1):2980. doi:

Type

Empty backbone

Use

Lentiviral; Gateway

Expression

Mammalian

Promoter

PGK

Available Since

Jan. 5, 2017

Availability

Academic Institutions and Nonprofits only

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CSIV-TRE-ASCL1

Plasmid

#154883

Purpose

Doxycycline-dependently expresses human ASCL1.

Depositor

Hideyuki Okano

Article

Nemoto et al Cells. 2020 Dec 22;10(1). pii: cells1001

Insert

ASCL1 (ASCL1 Human)

Use

Lentiviral

Available Since

Jan. 5, 2021

Availability

Academic Institutions and Nonprofits only

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IL10RB_pCSdest

Plasmid

#53799

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

IL10RB (IL10RB Human)

Expression

Mammalian

Promoter

SP6 and CMV

Available Since

March 27, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pET29a-hNABP2

Plasmid

#128307

Purpose

Express hNABP2 (a.k.a hSSB1 or SOSSB1) in E. coli with a C-terminal His-tag

Depositor

Yuliang Wu

Article

Vidhyasagar et al Biochim Biophys Acta. 2016 Feb;1860(2):3

Insert

Human Nucleic Acid Binding Protein 2 (hNABP2) (NABP2 Human)

Tags

His

Expression

Bacterial

Mutation

Y85C- please see depositor comments below

Promoter

T7

Available Since

Aug. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pMSCV-mCherry-Vav1

Plasmid

#50044

Purpose

Expression of mCherry-Vav1 in mammalian cells

Depositor

Hidde Ploegh

Article

Strijbis et al PLoS Pathog. 2013 Jun;9(6):e1003446. doi

Insert

Vav1 (Vav1 Mouse)

Use

Retroviral

Tags

mCherry

Expression

Mammalian

Promoter

pCMV

Available Since

Jan. 13, 2014

Availability

Academic Institutions and Nonprofits only

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pEVOL-AcKRS-CloDF

Plasmid

#127415

Purpose

Mutant of mmPylRS designed for incorporation of non-canonical amino acids (acyl-lysine derivatives) in to M13 bacteriophage

Depositor

Wenshe Liu

Article

Wang et al Angew Chem Int Ed Engl. 2019 Oct 28;58(4

Insert

Pyrrolysyl-tRNA synthetase/pyrrolysyl -tRNA pair

Expression

Bacterial

Promoter

araBAD

Available Since

April 15, 2022

Availability

Academic Institutions and Nonprofits only

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