We narrowed to 4,776 results for: Drosophila
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Plasmid#171566PurposeBacterial expression of HA-His-tagged anti-CXCR2 (human) recombinant llama nanobody (Nb127D01-HA-His)DepositorInsertNb127D01-HA-His (CXCR2 Human)
TagsHA-tag/His-tag and PelB signal peptideExpressionBacterialAvailable SinceJune 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET-26b-NbVHH05-ALFA-His
Plasmid#171569PurposeBacterial expression of ALFA-His-tagged anti-UBC6e (human) recombinant alpaca nanobody (NbVHH05-ALFA-His)DepositorInsertNbVHH05-ALFA-His (UBE2J1 Human)
TagsALFA-tag/His-tag and PelB signal peptideExpressionBacterialAvailable SinceJune 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDEST-Tol2-PA2-CMV-AB-mCh
Plasmid#160435PurposeExpresses human Amyloid Beta-mCherry in ZebrafishDepositorInsertHuman Amyloid Beta peptide (1-42) (APP Zebrafish, Human)
UseZebrafish expressionTagsmCherryPromoterCMVAvailable SinceOct. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUCIDT-attL1-Worm ABeta-attR5
Plasmid#160437PurposeEntry vector for cloning nematode-codon-optimized Amyloid Beta(1-42) using 2-fragment gateway recombinationDepositorInsertHomo sapiens amyloid beta precursor protein (APP Nematode, Human)
TagsCleavage signalExpressionBacterialMutationCodon-optimized for expression in C. elegansAvailable SinceOct. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
1073A(HomeR1)=pBac-[Pol-γ35.HL-decPol-γ35-p10]-[U6-gRNA#1(Pol-γ35)]-[3xp3-GFP-SV40]-Pol-γ35.HR-[Opie2-dsRed-SV40]
Plasmid#159676PurposePlasmid provides the HomeR#1 gene drive element harboring a rescue, gRNA#1, and 3xP3-eGFP that can be integrated via pBac and inserted at Pol-γ35 site #1 via HDR.DepositorInsertpBac-[Pol-γ35.HL-decPol-γ35-p10]-[U6-gRNA#1(Pol-γ35)]-[3xp3-GFP-SV40]-Pol-γ35.HR-[Opie2-dsRed-SV40]
ExpressionInsectAvailable SinceMarch 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDmelOR
Plasmid#126476PurposeVector for the functional expression of insect ORs in mammalian cells, with a human codon-optimized version of D.melanogaster Orco (hOrco) with an N-terminal myc tag and a β-globin/IgG chimeric intronDepositorInsertDmelOrco (Orco Fly)
TagsMycExpressionMammalianMutationcodon optimization for H. sapiens; includes a rec…PromoterCMVAvailable SinceJune 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
Donor_ANKRD1_KO_Puro
Plasmid#186667PurposeDonor plasmid to endogenously knock out the ANKRD1 in Hek293 cells. eEF1a promoter, puromycin and polyA sequence is inserted between two homology arms.DepositorInsertANKRD1 KO Puromycin (ANKRD1 Human)
ExpressionMammalianAvailable SinceAug. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBS-KDRT-STOP-loxP-3XP3::dsRed-loxP-STOP-KDRT-smGdP-10XHA
Plasmid#217511PurposeKD Recombinase dependent conditional smGdP-10XHA cassetteDepositorInsertKDRT-STOP-loxP-3XP3::dsRed-loxP-STOP-KDRT-smGdP-10XHA
TagssmGdP-10XHAExpressionInsectAvailable SinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBS-KDRT-STOP-loxP-3XP3::dsRed-loxP-STOP-KDRT-smGdP-10XOllas
Plasmid#217512PurposeKD Recombinase dependent conditional smGdP-10XOllas cassetteDepositorInsertKDRT-STOP-loxP-3XP3::dsRed-loxP-STOP-KDRT-smGdP-10XOllas
TagssmGdP-10XOllasExpressionInsectAvailable SinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
Donor_YTHDF2_KO_Hygro
Plasmid#186671PurposeDonor plasmid to endogenously knock out the YTHDF2 in Hek293 cells. Hygromycin and polyA sequence is inserted between two homology arms.DepositorInsertYTHDF2 KO Hygromycin (YTHDF2 Human)
ExpressionMammalianAvailable SinceAug. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
Donor_YTHDF2_KO_Puro
Plasmid#186670PurposeDonor plasmid to endogenously knock out the YTHDF2 in Hek293 cells. Puromycin and polyA sequence is inserted between two homology arms.DepositorInsertYTHDF2 KO Puromycin (YTHDF2 Human)
ExpressionMammalianAvailable SinceAug. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(-)-mRho.V5.mER.hOr47a
Plasmid#126472PurposeHuman codon-optimized version of D. melanogaster Or47a (hOr47a) with N-terminal tags derived from human Rhodopsin (mRho) and HCN1 (mER) for improved intracellular trafficking in mammalian cellsDepositorInsertOr47a (Or47a Fly)
TagsH. sapiens HCN1 106VNKFSL111 (mER), H. sapiens Rh…ExpressionMammalianMutationcodon optimization for H. sapiensPromoterCMVAvailable SinceJune 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
Gal4-entry+NLS
Plasmid#128013PurposeVector containing the Gal4-entry expression cassette with SV40 NLS and a separate expression cassette driving mCherry via the traffic jam enhancerDepositorInsertGal4 DBD-SV40NLS-3x-Flag
UseGal4 entry vector with nls to generate tethering …TagsGal4-DBD-SV40NLS-3xFlagAvailable SinceAug. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pENTR-E2F1
Plasmid#37141DepositorInsertE2F1 (E2f1 Fly)
UseGatewayAvailable SinceJune 21, 2012AvailabilityAcademic Institutions and Nonprofits only -
pJC-elav1.8kb-TALE1-VP64-P10
Plasmid#104609PurposeExpresses TALE1 under the control of a 1.8kb elav enhancer elementDepositorAvailable SinceJan. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMT-HGP-v3-NbVHH05-hIgG
Plasmid#171565PurposeExpresses hIgG-tagged anti-UBC6e (human) recombinant alpaca nanobody (NbVHH05-human IgG-TEV-Avi-His) in fly cellsDepositorInsertNbVHH05-human IgG-TEV-Avi-His (UBE2J1 Human)
TagsBiP signal peptide and hIgG1-hinge-N297A-TEV site…ExpressionInsectAvailable SinceJune 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
gRNAs[bTub+Sxl].1046B
Plasmid#112692Purposeexpress two gRNA targeting bTub & Sxl under dU6-3 promoterDepositorInsertU6.3-gRNA[bTub] and U6.3-gRNA[Sxl] (Sxl Fly)
UseCRISPRAvailable SinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJC-mhc2.4kb-TALE3-VP64-P10
Plasmid#104611PurposeExpresses TALE3 under the control of a 2.4kb mhc enhancer elementDepositorAvailable SinceJan. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmThor-V5His6
Plasmid#79408PurposeExpresses EGFP-tagged and C-terminal V5His6-tagged DmThor in S2 cellsDepositorInsertDmThor (Thor Fly)
UseSchneider 2 cell expressionTagsEGFP and V5-His6PromoterAc5 promoterAvailable SinceJuly 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
1073B(HomeR2) =pBac-[Pol-γ35.HL-decPol-γ35-p10]-[U6-gRNA#2(Pol-γ35)]-[3xp3-GFP-SV40]-Pol-γ35.HR-[Opie2-dsRed-SV40]
Plasmid#159677PurposePlasmid provides the HomeR#2 gene-drive element harboring a rescue, gRNA#2, and 3xP3-eGFP that can be integrated via pBac and inserted at Pol-γ35 site #2 via HDR.DepositorInsert[Pol-γ35.HL-decPol-γ35-p10]-[U6-gRNA#2(Pol-γ35)]-[3xp3-GFP-SV40]-Pol-γ35.HR-[Opie2-dsRed-SV40]
ExpressionInsectAvailable SinceMarch 8, 2021AvailabilityAcademic Institutions and Nonprofits only