We narrowed to 54,790 results for: uros

1. pLVX-Tight-Puro FLAG-SMAD4 TetON

   Plasmid

   #107129

   Purpose

   Inducible expression of Flag-tagged SMAD4 in mammalian cells

   Depositor

   Joan Massague

   Article

   David et al Cell. 2016 Feb 25;164(5):1015-30. doi: 1

   Insert

   SMAD4
   

   Use

   Lentiviral

   Tags

   FLAG

   Available Since

   Feb. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCK002_U6-Sa-sgRNA(mod)_EFS-SaCas9-2A-Puro_WPRE

   Plasmid

   #85452

   Purpose

   Lentiviral vector encoding modified SaCas9 system backbone bearing BsmBI site for new guide RNAs and puromycin selection marker.

   Depositor

   Aviv Regev

   Article

   Singer et al Cell. 2016 Sep 8;166(6):1500-1511.e9. do

   Insert

   U6-modified-sgRNA-Backbone-EFS-hSaCas9-2xNLS-2A-Puro
   

   Use

   CRISPR and Lentiviral

   Tags

   NLS

   Expression

   Mammalian

   Available Since

   April 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. MelanoTag-PURO (pLJC5-GPR143-mScarlet-3xHA)

   Plasmid

   #160369

   Purpose

   Stable expression of GPR143-mScarlet-3xHA fusion protein for the isolation of melanosomes.

   Depositor

   David Sabatini

   Article

   Adelmann et al Nature. 2020 Nov 18. pii: 10.1038/s41586

   Insert

   GPR143 (GPR143 Human)
   

   Use

   Lentiviral

   Tags

   mScarlet-3xHA

   Available Since

   Nov. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pLVX-EF1a-LAMP-1-mGFP-IRES-Puromycin

   Plasmid

   #134868

   Purpose

   Expresses EGFP-tagged lysosomal marker

   Depositor

   David Andrews

   Article

   Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

   Insert

   Lysosomal-associated membrane protein 1 (LAMP1 Human)
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   March 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. tetO-GFPnls-mPGK-rtTA-IRES-Puro

   Plasmid

   #69545

   Purpose

   Tet-On system expressing GFP tagged with 2x nuclear localization signals.

   Depositor

   Charles Gersbach

   Insert

   eGFP
   

   Use

   Lentiviral

   Tags

   2x nuclear localization sequence and T2A "sk…

   Available Since

   April 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pBABE-MCS(extended)-Puro (empty vector)

   Plasmid

   #172603

   Purpose

   Empty retroviral vector for the constitutive, near-physiological expression of a gene of interest and a puromycin resistance marker in mammalian cells.

   Depositor

   Michele Pagano

   Article

   Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

   Type

   Empty backbone

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   The multiple cloning site (MCS) has been edited/e…

   Available Since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA-puro-TagBFP-β-ACTIN-3′UTR-24xMS2

   Plasmid

   #205161

   Purpose

   express β-ACTIN-3′UTR-24xMS2

   Depositor

   Weirui Ma

   Article

   Hu et al Elife. 2023 Mar 3;12:e82178. doi: 10.755

   Insert

   β-ACTIN-3′UTR-24xMS2
   

   Tags

   tagBFP

   Expression

   Bacterial and Mammalian

   Promoter

   CMV

   Available Since

   Oct. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pU6-sgRNA Ef1alpha Puro-T2A-GFP

   Plasmid

   #111596

   Purpose

   sgRNA Ef1alpha Puro-T2A-GFP

   Depositor

   Jonathan Weissman

   Article

   Horlbeck et al Cell. 2018 Aug 9;174(4):953-967.e22. doi

   Insert

   mU6-sgRNA
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Feb. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLVX-EF1a-EGFP-RAB7A-IRES-Puromycin

   Plasmid

   #133027

   Purpose

   Expresses EGFP-tagged late endosome marker

   Depositor

   David Andrews

   Article

   Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

   Insert

   RAB7A (RAB7A Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   Human elongation factor 1 alpha

   Available Since

   Feb. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLV-EF1a-IRES-Puro GFP-3xFLAG

    Plasmid

    #201636

    Purpose

    Over-expression of 3xFLAG-tagged GFP

    Depositor

    Vamsi Mootha, Alexis Jourdain

    Article

    Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

    Insert

    GFP
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Available Since

    July 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLVX-EF1a-EGFP-RAB5A-IRES-Puromycin

    Plasmid

    #134858

    Purpose

    Expresses EGFP-tagged early endosome marker

    Depositor

    David Andrews

    Article

    Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

    Insert

    Ras-related protein 5A
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    March 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLenti-PGK-Puro-TK-NLS-mTurq2-PCNA

    Plasmid

    #118617

    Purpose

    Lentiviral expression of mTurq2 tagged PCNA

    Depositor

    Jean Cook

    Article

    Grant et al Cell Cycle. 2018;17(21-22):2496-2516. do

    Insert

    TK-NLS-mTurq2-PCNA (PCNA Human)
    

    Use

    Lentiviral

    Tags

    mTurq2

    Expression

    Mammalian

    Promoter

    hPGK

    Available Since

    May 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. PB/pHSP-tTA-TRE-tTA-pEF1α-PuroR

    Plasmid

    #206213

    Purpose

    PiggyBac-based transposon vector plasmid which encodes an expression unit of tetracycline-responsive transactivator under control of HSP promoter with transcriptional amplification

    Depositor

    Masamichi Kamihira

    Article

    Kitano et al Cytotechnology. 2021 Jun;73(3):353-362.

    Insert

    tetracycline-responsive transactivator
    

    Expression

    Mammalian

    Available Since

    Oct. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. RRL.sin.cPPT.SFFV/Flag-Firefly luciferase.IRES-puro.WPRE (MT05)

    Plasmid

    #139446

    Purpose

    Lentiviral vector genome to ectopically express Firefly luciferase

    Depositor

    Caroline Goujon

    Article

    Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

    Insert

    FLAG-tag Firefly luciferase
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    SFFV

    Available Since

    Aug. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pBabe-Puro-IKBalpha-mut (super repressor)

    Plasmid

    #15291

    Depositor

    William Hahn

    Article

    Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

    Insert

    inhibitor of Kappa B alpha (NFKBIA Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    S32A/S36A: “super-repressor” allele (resistant to…

    Available Since

    July 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pKLV2.2-h7SKgRNA5(SapI)-hU6gRNA5(BbsI)-PGKpuroBFP-W

    Plasmid

    #72666

    Purpose

    Lentiviral dual CRISPR gRNA expression vector (human 7SK and human U6 promoters)

    Depositor

    Kosuke Yusa

    Article

    Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

    Insert

    h7SKgRNA cassette, hU6gRNA cassette, PGKpuroBFP cassette, WPRE
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Oct. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLVX-EF1a-EGFP-TA Golgin84-IRES-Puromycin

    Plasmid

    #133028

    Purpose

    Expresses EGFP-tagged Golgi apparatus marker

    Depositor

    David Andrews

    Article

    Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

    Insert

    Golgin84 (GOLGA5 Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    deleted amino acids 1-673

    Promoter

    Human elongation factor 1 alpha

    Available Since

    Feb. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLenti-C-Myc-DDK-IRES-Puro HNRNPQ

    Plasmid

    #200729

    Purpose

    Lentiviral expression of Flag-Tagged human HNRNPQ

    Depositor

    Claudio Alarcón

    Insert

    HNRNPQ (SYNCRIP Human)
    

    Use

    Lentiviral

    Tags

    Myc-DDK from backbone plasmid

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLVX-EF1a-EGFP-RAB11B-IRES-Puromycin

    Plasmid

    #134860

    Purpose

    Expresses EGFP-tagged recycling endosome marker

    Depositor

    David Andrews

    Article

    Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

    Insert

    RAB11B (RAB11B Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    March 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. TLCV2-ApaLI-FLAG-T2A-mTagBFP2-Puro

    Plasmid

    #211462

    Purpose

    Lentivirus backbone expressing a doxycycline-inducible mitochondrial localized ApaLI

    Depositor

    Agnel Sfeir

    Article

    Fu et al Mol Cell. 2023 Oct 8:S1097-2765(23)00753

    Insert

    ApaLI
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    TRE

    Available Since

    Dec. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge