We narrowed to 4,836 results for: HRE
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Plasmid#215883PurposeThis plasmid encodes luciferase which has microRNA binding sites in 3'UTR for detecting the microRNA silencing activity.DepositorInsertthree tandem repeats of seed-matched (SM) sequence of miR-376a1(3p)-I-type (MIR376A1 Human)
ExpressionMammalianAvailable SinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-1-miR-589(5p)-I-type
Plasmid#215884PurposeThis plasmid encodes luciferase which has microRNA binding sites in 3'UTR for detecting the microRNA silencing activity.DepositorInsertthree tandem repeats of seed-matched (SM) sequence of miR-589(5p)-I-type (MIR589 Human)
ExpressionMammalianAvailable SinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-1-miR-589(3p)-I-type
Plasmid#215885PurposeThis plasmid encodes luciferase which has microRNA binding sites in 3'UTR for detecting the microRNA silencing activity.DepositorInsertthree tandem repeats of seed-matched (SM) sequence of miR-589(3p)-I-type (MIR589 Human)
ExpressionMammalianAvailable SinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFB-D3-ASK1
Plasmid#226619PurposeCo-expression of D3 and ASK1 complex in insect cellsDepositorInsertsTagsHis tag followed with three copies of MsybExpressionInsectMutationThe flexible loop in D3 (Q478-D515) can be remove…PromoterPolyhedrinAvailable SinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
T40A+Q54A
Plasmid#217913PurposeA plasmid encoding the hydrogel-forming protein PXP with both the T40A and Q54A coil mutationsDepositorInsertPXP T40A+Q54A
Tags6x His (N and C term)ExpressionBacterialMutationThreonine at position 41 and 253 mutated to Alani…PromoterT5Available SinceJune 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAS_4xKBstTyrT(CUA)_EF1_sfGFP 102TAG 150TAA
Plasmid#174895Purposeamber and ochre dual suppression reporter sfGFP 102TAG 150 TAA expression, with BstTyr(CUA) amber suppressor tRNA cassetteDepositorInsertsfGFP
ExpressionMammalianMutation102TAG 150TAA in sfGFPPromoterEF1Available SinceNov. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA 3.1 - circMAN2A1
Plasmid#198837PurposeThis contains the 400nt isoform of exons two and three from MAN2A1 circular RNA, 3X Flag tag inserted in exon twoDepositorInsertcircular mannosidase alpha class 2A member 1
ExpressionMammalianMutationcircular RNA composed by 2 exonsPromoterCMVAvailable SinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-RIPK3[V458D][Q459D][V460D][G461D]-FLAG
Plasmid#199327Purposemutated RHIM motive (interaction active)DepositorInsertReceptor-interacting serine/threonine-protein kinase 3 (RIPK3 Human)
TagsFLAGExpressionMammalianMutationchanged V458, Q459,V460 and G461 to aspartic acidAvailable SinceJune 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmNot1-7xmut-dsRNAres_U
Plasmid#147709PurposeInsect Expression of DmNot1-7xmut-dsRNAresDepositorInsertDmNot1-7xmut-dsRNAres (Not1 Fly)
ExpressionInsectMutation10 silent mutations and three non silent N605S, K…Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmNot1-8xmut-dsRNAres_U
Plasmid#147710PurposeInsect Expression of DmNot1-8xmut-dsRNAresDepositorInsertDmNot1-8xmut-dsRNAres (Not1 Fly)
ExpressionInsectMutation10 silent mutations and three non silent N605S, K…Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmeIF4Ga_578-650-CG32016-E_T
Plasmid#147588PurposeInsect Expression of DmeIF4Ga_578-650-CG32016-EDepositorInsertDmeIF4Ga_578-650-CG32016-E (eIF4G1 Fly)
ExpressionInsectMutationCG32016-E 5 silent and three no silent mutations …Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCIneo-lambdaN-HA-HsSmg7-G100E-siRNAres-shRNAres_S
Plasmid#147461PurposeMammalian Expression of HsSmg7-G100E-siRNAres-shRNAresDepositorInsertHsSmg7-G100E-siRNAres-shRNAres (SMG7 Human)
ExpressionMammalianMutationthree silent mutation G216A, T1185C and A1521G an…Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCIneo-lambdaN-HA-HsSmg7-K66ER163E-siRNAres-shRNAres_S
Plasmid#147462PurposeMammalian Expression of HsSmg7-K66ER163E-siRNAres-shRNAresDepositorInsertHsSmg7-K66ER163E-siRNAres-shRNAres (SMG7 Human)
ExpressionMammalianMutationthree silent mutation G216A, T1185C and A1521G an…Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCIneo-lambdaN-HA-HsSmg7-T103E-siRNAres_S
Plasmid#147464PurposeMammalian Expression of HsSmg7-T103E-siRNAres-shRNAresDepositorInsertHsSmg7-T103E-siRNAres-shRNAres (SMG7 Human)
ExpressionMammalianMutationthree silent mutation G216A, T1185C and A1521G an…Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pN1-HsSmg5-Q123E-siRNAres-V5-SBP-MBP_S
Plasmid#147471PurposeMammalian Expression of HsSmg5-Q123E-siRNAresDepositorInsertHsSmg5-Q123E-siRNAres (SMG5 Human)
ExpressionMammalianMutationthree non silent mutation A254G (K85R), T980C (L3…Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGADT7-γ1
Plasmid#197078PurposeExpression of GAL4 transcriptional activation domain (AD)-AP-1 γ1 fusion protein in yeast (yeast two-hybrid or yeast three-hybrid assays)DepositorInsertAP-1 γ1 (Ap1g1 Mouse)
TagsGAL4 transcriptional activation domain (AD) fragm…ExpressionYeastPromoterADH1Available SinceMarch 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCEP4_HLA-cmyc-GRM3 C127A T174A-VC
Plasmid#140457PurposeMammalian expression plasmid for c-myc-tagged mGluR3 C127A and T174A mutant fused to the C-terminal half of split fluorescent Venus (VC)DepositorInsertGRM3 (GRM3 Human)
TagsSignal/leader sequence from HLA class I histocomp…ExpressionMammalianMutationCysteine 127 to alanine, Threonine 174 to alanineAvailable SinceJuly 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS2361_Tier3(SB)-rtTA-YPet_PuroR
Plasmid#169657PurposeTier-3 vector for stable stable integration of up to three cassettes via sleeping beauty transposase including a YPet marker and PuroR resistance gene and a PPGK-driven rtTA transactivator (5'ITR-A1-pA::PPGK-rtTA-pA::PRPBSA-YPet-p2A-PuroR-pA-3'ITR)DepositorInsertPPGK-driven rtTA expression and PRPBSA-driven YPet and PuroR expression
ExpressionMammalianPromoterPRPBSAAvailable SinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS2363_Tier3(PB)-rtTA-YPet_PuroR
Plasmid#169659PurposeTier-3 vector for stable stable integration of up to three cassettes via Piggy Bac transposase including a YPet marker and PuroR resistance gene and a PPGK-driven rtTA transactivator (5'ITR-A1-pA::PPGK-rtTA-pA::PRPBSA-YPet-p2A-PuroR-pA-3'ITR)DepositorInsertPPGK-driven rtTA expression and PRPBSA-driven YPet and PuroR expression
ExpressionMammalianPromoterPRPBSAAvailable SinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmEDC3_102-330-V5His6_D
Plasmid#146129PurposeInsect Expression of DmEDC3_102-330DepositorInsertDmEDC3_102-330 (Edc3 Fly)
ExpressionInsectMutationone silent mutation A456G and three mutations N16…Available SinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only