We narrowed to 90,020 results for: Mal;

1. pAG156 Mito-FAST

   Plasmid

   #130723

   Purpose

   Expresses FAST (also called YFAST) fused to a mitochondria targeting sequence (mito) in mammalian cells

   Depositor

   Arnaud Gautier

   Article

   Plamont et al Proc Natl Acad Sci U S A. 2016 Jan 19;11

   Insert

   Mito-FAST
   

   Tags

   Myc-tag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-sPDGFRalpha-Fc

   Plasmid

   #136438

   Purpose

   Mammalian expression plasmid for soluble ectodomain of PDGFR-alpha fused to IgG1 Fc

   Depositor

   Erik Procko

   Article

   Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.

   Insert

   PDGFRalpha (PDGFRA Human)
   

   Tags

   Fc region of IgG1 (a.a. Asp-221 to Lys-447) and L…

   Expression

   Mammalian

   Mutation

   Extracellular domain of PDGFR-alpha (a.a. 1-524)

   Promoter

   CMV

   Available Since

   July 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCEP4-myc-S (Wuhan variant)

   Plasmid

   #169846

   Purpose

   Mammalian expression plasmid for S of SARS-CoV-2 (Wuhan variant) with N-terminal c-myc tag

   Depositor

   Erik Procko

   Article

   Zhang et al Nat Chem Biol. 2022 Mar;18(3):342-351. d

   Insert

   Protein S
   

   Tags

   HA leader and c-myc tag

   Expression

   Mammalian

   Mutation

   Codon-optimized for human cell expression.

   Promoter

   CMV

   Available Since

   Jan. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAG109 H2B-FAST

   Plasmid

   #130722

   Purpose

   Expresses FAST (also called YFAST) fused to zebrafish histone H2B in mammalian cells

   Depositor

   Arnaud Gautier

   Article

   Plamont et al Proc Natl Acad Sci U S A. 2016 Jan 19;11

   Insert

   H2B-FAST
   

   Tags

   myc-tag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTBL210

   Plasmid

   #126451

   Purpose

   Expresses Cas9-XTEN-TdT in mammalian cells.

   Depositor

   Chang Liu

   Article

   Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

   Insert

   Cas9-XTEN-TdT (DNTT Streptococcus pyogenes, Human)
   

   Expression

   Mammalian

   Mutation

   See depositor comments below

   Promoter

   CMV

   Available Since

   June 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. dCas9-NLS-gs10-cLYN268

   Plasmid

   #188259

   Purpose

   Plasmid ensures constitutive expression of the C-terminal fragment of split LYN kinase domain (aa 239-267), fused to the catalytically inactive Cas9 (dCas9) protein, a flexible GS linker and the SV40 NLS signal at the N-terminus

   Depositor

   Roman Jerala

   Article

   Rihtar et al Nat Chem Biol. 2022 Sep 26. doi: 10.1038

   Insert

   cLYN268 (LYN Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Oct. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pINDUCER-21-LCOR

   Plasmid

   #97038

   Purpose

   Expresses LCOR in mammalian cells

   Depositor

   George Daley

   Article

   Sugimura et al Nature. 2017 May 17. doi: 10.1038/nature

   Insert

   Ligand Dependent Nuclear Receptor Corepressor (LCOR Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   Tet on

   Available Since

   Sept. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. LV_EF1a_MEK1(S218D,S222D)-P2A-Hygro_Barcode

   Plasmid

   #170240

   Purpose

   Barcoded lentiviral vector to express MEK1 (S218D, S222D) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

   Depositor

   Prashant Mali

   Article

   Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

   Insert

   MAP2K1 (MAP2K1 Human)
   

   Use

   Lentiviral

   Mutation

   S218D, S222D

   Promoter

   EF1a

   Available Since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. EF1a_MYC Thr58Ala_P2A_Hygro_Barcode

   Plasmid

   #120513

   Purpose

   Barcoded lentiviral vector to express MYC Thr58Ala in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

   Depositor

   Prashant Mali

   Article

   Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

   Insert

   MYC Thr58Ala (MYC Human)
   

   Use

   Lentiviral

   Mutation

   Point mutation changing Threonine to Alanine at a…

   Promoter

   EF1a

   Available Since

   Feb. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. EF1a_MYC deltaNLS_P2A_Hygro_Barcode

    Plasmid

    #120506

    Purpose

    Barcoded lentiviral vector to express c-MYC deltaNLS in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Insert

    MYC H84NLS (MYC Human)
    

    Use

    Lentiviral

    Mutation

    Deletion of nuclear localization signal sequence

    Promoter

    EF1a

    Available Since

    Oct. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1(-)-C2C1-ErNaR-TS-EYFP-ES

    Plasmid

    #209851

    Purpose

    Expression of human codon-optimized light-driven sodium pump ErNaR in mammalian cells

    Depositor

    Volker Busskamp

    Article

    Podoliak et al Nat Commun. 2024 Apr 10;15(1):3119. doi:

    Insert

    light-driven sodium pump ErNaR
    

    Tags

    EYFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEGFP C1-Eps8 ∆cap

    Plasmid

    #74786

    Purpose

    mammalian expression of the capping activity mutant Eps8 fused to GFP

    Depositor

    Giorgio Scita

    Article

    Hertzog et al PLoS Biol. 2010 Jun 1;8(6):e1000387. doi

    Insert

    Eps8 (Eps8 Mouse)
    

    Expression

    Mammalian

    Mutation

    V689D, L693D

    Available Since

    May 3, 2016

    Availability

    Academic Institutions and Nonprofits only

13. pTE4889

    Plasmid

    #88905

    Purpose

    Expresses FnCpf1 crRNA and inactive/dead, humanized FnCpf1 nuclease

    Depositor

    Ervin Welker

    Article

    Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

    Inserts

    Fn crRNA
    hFnCpf1(D917A)
    

    Use

    CRISPR

    Tags

    3xHA and NLS

    Expression

    Mammalian

    Promoter

    CMV and human U6

    Available Since

    April 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLEX-uORF-HA-birA*-H-Ras(G12V)-IRES-Puro

    Plasmid

    #120560

    Purpose

    Expresses HA-birA*-H-Ras G12V mutant fusion protein in mammalian cells & for virus production

    Depositor

    Paul Khavari

    Article

    Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

    Insert

    HRAS (HRAS Human)
    

    Use

    Lentiviral

    Tags

    HA-birA*

    Expression

    Mammalian

    Mutation

    G12V

    Promoter

    CMV

    Available Since

    Jan. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGEX2T-TUBG1

    Plasmid

    #171967

    Purpose

    pGEX2T-TUBG1 bacterial expression plasmid for production of recombinant GST fusion protein

    Depositor

    Maria Alvarado-Kristensson

    Article

    Corvaisier et al Commun Biol. 2021 Jun 22;4(1):767. doi:

    Insert

    TUBG1 (TUBG1 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    R275H (Please see depositor comments)

    Available Since

    July 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEGFP C1-Eps8 ∆bund

    Plasmid

    #74889

    Purpose

    mammalian expression of the bundling activity mutant Eps8 fused to GFP

    Depositor

    Giorgio Scita

    Article

    Hertzog et al PLoS Biol. 2010 Jun 1;8(6):e1000387. doi

    Insert

    Eps8 (Eps8 Mouse)
    

    Expression

    Mammalian

    Mutation

    L757A, K759A

    Available Since

    May 3, 2016

    Availability

    Academic Institutions and Nonprofits only

17. pTBL469

    Plasmid

    #126458

    Purpose

    Expresses Cas9-15xFlag-TdT in mammalian cells.

    Depositor

    Chang Liu

    Article

    Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

    Inserts

    Cas9
    15x Flag
    TdT (DNTT Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. EF1a_MYC Ser62Ala_P2A_Hygro_Barcode

    Plasmid

    #120514

    Purpose

    Barcoded lentiviral vector to express MYC Ser62Ala in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Insert

    MYC Ser62Ala (MYC Human)
    

    Use

    Lentiviral

    Mutation

    Point mutation changing Serine to Alanine at amin…

    Promoter

    EF1a

    Available Since

    Feb. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. LV_EF1a_Stat3(A662C,N664C,V667L)-P2A-Hygro_Barcode

    Plasmid

    #170254

    Purpose

    Barcoded lentiviral vector to express Stat3 (A662C, N664C, V667L) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

    Depositor

    Prashant Mali

    Article

    Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

    Insert

    STAT3 (STAT3 Human)
    

    Use

    Lentiviral

    Mutation

    A662C, N664C, V667L

    Promoter

    EF1a

    Available Since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. Mouse DsRed-Monomer CASP9 C325A Mutant

    Plasmid

    #198401

    Purpose

    Expresses Mouse DsRed-Monomer CASP9 C325A Mutant Fusion Protein in Mammalian Cells

    Depositor

    Hannah Rabinowich

    Article

    Han et al Autophagy. 2021 Jun;17(6):1393-1409. doi

    Insert

    Mouse Caspase-9 C325A Mutant (Casp9 Mouse)
    

    Tags

    DsRed-monomer

    Expression

    Mammalian

    Mutation

    Changed Cysteine 325 to Alanine to eliminate prot…

    Promoter

    CMV

    Available Since

    June 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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