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We narrowed to 17,478 results for: IGH@

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Showing: 11151 - 11200 of 17478 results

  3. pYPQ-dAaCas12b-Act3.0

    Plasmid
    #158413
    Purpose
    CRISPR-Act3.0 system containing dAaCas12b-VP64 fusion protein, T2A linked MS2-SunTag fusion protein, and ScFv-sfGFP-2xTAD activator.
    Depositor
    Yiping Qi
    Article
    Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:
    Insert
    dAaCas12b-VP64-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
    Use
    CRISPR
    Expression
    Plant
    Available Since
    July 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    158413-plasmid-map-sequence-id-337077

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  7. MSP2372

    Plasmid
    #73196
    Purpose
    Human expression plasmid for SpCas9(R661A/Q695A/Q926A) variant: CMV-T7-humanSpCas9(R661A, Q695A, Q926A)-NLS-3xFLAG
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1
    Insert
    mammalian codon-optimized Streptococcus pyogenes Cas9 (R661A/Q695A/Q926A)-NLS-3xFlag
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Mammalian
    Mutation
    R661A, Q695A, and Q926A in SpCas9
    Promoter
    CMV
    Available Since
    Feb. 22, 2016
    Availability
    Academic Institutions and Nonprofits only
    73196-plasmid-map-sequence-id-134659

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  10. TALEN-CCR5R18-CtermQ7

    Plasmid
    #51443
    Purpose
    Expresses TALEN targeting right CCR5A site (R18) with Q7 C-terminal domain
    Depositor
    David Liu
    Article
    Guilinger et al Nat Methods. 2014 Apr;11(4):429-35. doi:
    Insert
    TALEN CCR5 Right (R18) Q7 C-term
    Use
    TALEN
    Tags
    3xFLAG and Fok1 KK variant
    Expression
    Mammalian
    Mutation
    K777Q, K778Q, K788Q, R789Q, R792Q, R793Q and R801Q
    Promoter
    CMV
    Available Since
    April 1, 2014
    Availability
    Academic Institutions and Nonprofits only
    51443-plasmid-map-sequence-id-78480

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  12. p1.1-eGFP

    Plasmid
    #162738
    Purpose
    Fluorescent reporter for CHO cells expression studies
    Depositor
    Ivan Vorobiev
    Article
    Orlova et al BMC Biotechnol. 2014 Jun 14;14:56. doi:
    Insert
    enhanced green fluorescent protein
    Expression
    Mammalian
    Mutation
    consensus Kozak sequence (GCCGCCATGG) added befor…
    Promoter
    CHO EEF1A1 (Translation Elongation Factor 1 Alpha…
    Available Since
    July 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    162738-plasmid-map-sequence-id-337724

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  16. MBC1-PuroIII

    Plasmid
    #153419
    Purpose
    MSCV backbone with murine TCR-alpha signal peptide and murine TCR-beta1 constant region (TRBC1) for expression of TCR genes
    Depositor
    Maki Nakayama
    Article
    Anderson et al Proc Natl Acad Sci U S A. 2021 Oct 12;11
    Insert
    murine TCR-alpha signal peptide and murine TCR-beta1 constant region (TRBC1)
    Use
    Retroviral
    Expression
    Mammalian
    Available Since
    Aug. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    153419-plasmid-map-sequence-id-306221

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  26. pET-30a(+)-Syk-tSH2-PM

    Plasmid
    #111271
    Purpose
    express murine Syk tandem SH2 domains (Ser 8 to Gln 264), with Y130E mutation to mimic phosphorylation, in E.coli strain Rosetta 2 (DE3)
    Depositor
    Carol Post
    Article
    Feng et al Phys Chem Chem Phys. 2016 Feb 17;18(8):5
    Insert
    murine Syk tandem SH2 domains (Ser 8 to Gln 264), with Y130E mutation to mimic phosphorylation (Syk Mouse)
    Expression
    Bacterial
    Mutation
    Y130E mutation to mimic phosphorylation
    Promoter
    T7 promoter
    Available Since
    July 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    111271-plasmid-map-sequence-id-215610

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  29. pX330_ACTB-i1 sgRNA / hSpCas9

    Plasmid
    #172825
    Purpose
    Mammalian expression of a sgRNA targeting the intron 1 position 1 of ACTB (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.
    Depositor
    Haining Zhong
    Article
    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1
    Insert
    sgRNA targeting the intron 1 of ACTB under the U6 promotor and hSpCas9 under the CAG promotor
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Aug. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    172825-plasmid-map-sequence-id-340913

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  33. pBT3019

    Plasmid
    #122541
    Purpose
    Expression of a C. elegan codon optimized florescent protein (CemOrange2) fused to a peroxisome target peptide (SKL) under the intestinal specific promoter nhx-2
    Depositor
    Gary Silverman
    Article
    Thomas et al PLoS One. 2019 Mar 26;14(3):e0214257. do
    Insert
    Pnhx-2_CemOrange2::SKL
    Tags
    SKL
    Expression
    Bacterial
    Promoter
    Pnhx-2
    Available Since
    March 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    122541-plasmid-map-sequence-id-238135

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  36. pYPQ269C2

    Plasmid
    #164716
    Purpose
    Gateway entry clone (attL1 & attR5) hAID-CBE_V04 for C-T base editing
    Depositor
    Yiping Qi
    Article
    Ren et al Plant Biotechnol J. 2021 May 27. doi: 10
    Insert
    hAID-zCas9(D10A)-UGI-T2A-MS2-UGI
    Use
    CRISPR; Gateway compatible haid-cbe_v04 entry clo…
    Expression
    Plant
    Available Since
    May 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    164716-plasmid-map-sequence-id-325386

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  40. pX330 _HITI donor B6

    Plasmid
    #172847
    Purpose
    DRS-2 sgRNA expression under a U6 promotor and HITI donor B6 (Zhong et al, eLife 2021), mEGFP translational phase (1-1), excised by DRS-2 sgRNA (with SpCas9).
    Depositor
    Haining Zhong
    Article
    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1
    Insert
    DRS-2 sgRNA and HITI donor (phase 1) encoding mEGFP
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Aug. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    172847-plasmid-map-sequence-id-341030

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  44. GFP-BICD2N

    Plasmid
    #64070
    Purpose
    N-terminal 400 amino acids of mouse BICD2 codon optimized for Sf9 expression with a N-terminal GFP tag and a TEV-cleavable His-ZZ tag for purification (GFP-BICD2N)
    Depositor
    Andrew Carter
    Article
    Schlager et al EMBO J. 2014 Sep 1;33(17):1855-68. doi:
    Insert
    BICD2N (1-400) (Bicd2 Mouse)
    Tags
    GFP and His-ZZ-TCS
    Expression
    Insect
    Mutation
    Codon optimized for Sf9 expression
    Available Since
    Sept. 9, 2015
    Availability
    Academic Institutions and Nonprofits only
    64070-plasmid-map-sequence-id-123551

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Showing: 11151 - 11200 of 17478 results