We narrowed to 17,914 results for: REV

1. CS2-Myc-mASUN

   Plasmid

   #47044

   Purpose

   Expression of tagged-mouse ASUN

   Depositor

   Laura Lee

   Article

   Jodoin et al Mol Biol Cell. 2012 Dec;23(24):4713-24.

   Insert

   Asunder (Asun Mouse)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 10, 2013

   Availability

   Academic Institutions and Nonprofits only

2. Neo1.c-AP-His

   Plasmid

   #71965

   Purpose

   Expresses the extracellular region of the Neogenin 1, isoform c protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

   Depositor

   Woj Wojtowicz

   Article

   Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

   Insert

   Neo1.c (Neo1 Mouse)
   

   Use

   Tags

   AP-His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. Plxnc1-AP-His

   Plasmid

   #72005

   Purpose

   Expresses the extracellular region of the PlexinC1 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

   Depositor

   Woj Wojtowicz

   Article

   Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

   Insert

   Plxnc1 (Plxnc1 Mouse)
   

   Use

   Tags

   AP-His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. Unc5c.z-Fc-His

   Plasmid

   #72182

   Purpose

   Expresses the extracellular region of the Unc5C, isoform z protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

   Depositor

   Woj Wojtowicz

   Article

   Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

   Insert

   Unc5c.z (Unc5c Mouse)
   

   Use

   Tags

   Fc-His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. Sdk2-AP-His

   Plasmid

   #72009

   Purpose

   Expresses the extracellular region of the Sdk2 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

   Depositor

   Woj Wojtowicz

   Article

   Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

   Insert

   Sdk2 (Sdk2 Mouse)
   

   Use

   Tags

   AP-His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. Plxnb3-AP-His

   Plasmid

   #72004

   Purpose

   Expresses the extracellular region of the PlexinB3 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

   Depositor

   Woj Wojtowicz

   Article

   Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

   Insert

   Plxnb3 (Plxnb3 Mouse)
   

   Use

   Tags

   AP-His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. KIF5C(1-560)-EF(C)

   Plasmid

   #61675

   Purpose

   Non-fluorescent, truncated kinesin-1 fused to EF Hand linker

   Depositor

   Kristen Verhey

   Article

   Norris et al J Cell Biol. 2014 Nov 10;207(3):393-406.

   Insert

   KIF5C(1-560)-EF(C) (Kif5c Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Unc5d.z-Fc-His

   Plasmid

   #72185

   Purpose

   Expresses the extracellular region of the Unc5D, isoform z protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

   Depositor

   Woj Wojtowicz

   Article

   Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

   Insert

   Unc5d.z (Unc5d Mouse)
   

   Use

   Tags

   Fc-His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pRS424-TUB2 - human TUBB6 Cterminal tail

   Plasmid

   #60403

   Purpose

   Expression of chimeric yeast beta tubulin (TUB2) - human beta5 tubulin Cterminal tail (TUBB6) using a GAL promoter

   Depositor

   Ron Vale

   Article

   Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do

   Insert

   TUB2
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   TUB2 amino acids 429 - end, replaced with human T…

   Promoter

   GAL

   Available since

   May 6, 2015

   Availability

   Academic Institutions and Nonprofits only

10. KIF5C(1-560)-IA

    Plasmid

    #61671

    Purpose

    Non-fluorescent, truncated kinesin-1 fused to IA linker

    Depositor

    Kristen Verhey

    Article

    Norris et al J Cell Biol. 2014 Nov 10;207(3):393-406.

    Insert

    KIF5C(1-560)-IA (Kif5c Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA3-Rog

    Plasmid

    #24429

    Depositor

    Bruce Conklin

    Article

    Scearce-Levie et al BMC Biol. 2005 . 3():3.

    Insert

    RASSL (OPRK1 Human)
    

    Use

    Tags

    FLAG and GFP

    Expression

    Mammalian

    Mutation

    mutations in extracellular loops 2 (see comments …

    Promoter

    Available since

    June 16, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA3.2/V5 hsa-mir-220a

    Plasmid

    #26314

    Depositor

    David Bartel

    Article

    Chiang et al Genes Dev. 2010 May 15. 24(10):992-1009.

    Insert

    hsa-mir-220a (MIR220A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 29, 2010

    Availability

    Academic Institutions and Nonprofits only

13. pBB3-ScVip1-536-1107-H548A

    Plasmid

    #238319

    Purpose

    ScVip1-536-1107-H548A protein expression in insect cells

    Depositor

    Michael Hothorn

    Article

    Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

    Insert

    VIP1 (VIP1 Budding Yeast)
    

    Use

    Tags

    His10-Tag, Twin-Strep-tag, TEV

    Expression

    Insect

    Mutation

    ScVip1-536-1107-H548A

    Promoter

    Available since

    June 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. TDH3pro-rtTA-tetO7pro-T7pol-pRS413

    Plasmid

    #239293

    Purpose

    Expresses the reverse tetracycline activator (rtTA) and T7 polymerase; Plasmid #1 of the tet-on overexpression system

    Depositor

    Anita Hopper

    Article

    Nostramo et al Mol Cell. 2025 Feb 20;85(4):726-741.e6.

    Inserts

    T7 polymerase with NLS and P278L
    reverse tetracycline transactivator (rtTA)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Contains M2-SE-G72P mutations, described in Addge…

    Promoter

    TDH3 and tetracycline-responsive element (TRE) an…

    Available since

    June 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCDF_AaRaf1/Raf2/BSD2

    Plasmid

    #229515

    Purpose

    Expresses Anthoceros agrestis chaperones: Raf1,Raf2,BSD2

    Depositor

    Laura Gunn

    Article

    Oh et al Mol Plant. 2024 Dec 2;17(12):1833-1849.

    Inserts

    Rubisco accumulation factor 1
    Rubisco accumulation factor 2
    BSD2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    N terminus chloroplast transit peptide truncation

    Promoter

    T7

    Available since

    June 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pBB3-ScVip1-536-1107

    Plasmid

    #238312

    Purpose

    ScVip1-536-1107 protein expression in insect cells

    Depositor

    Michael Hothorn

    Article

    Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

    Insert

    VIP1 (VIP1 Budding Yeast)
    

    Use

    Tags

    His10-Tag, Twin-Strep-tag, TEV

    Expression

    Insect

    Mutation

    ScVip1-536-1107

    Promoter

    Available since

    June 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pBB3-ScVip1-536-1107-K554A

    Plasmid

    #238329

    Purpose

    ScVip1-536-1107-K554A protein expression in insect cells

    Depositor

    Michael Hothorn

    Article

    Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

    Insert

    VIP1 (VIP1 Budding Yeast)
    

    Use

    Tags

    His10-Tag, Twin-Strep-tag, TEV

    Expression

    Insect

    Mutation

    ScVip1-536-1107-K554A

    Promoter

    Available since

    June 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pBB3-ScVip1-536-1107-R547A

    Plasmid

    #238318

    Purpose

    ScVip1-536-1107-R547A protein expression in insect cells

    Depositor

    Michael Hothorn

    Article

    Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

    Insert

    VIP1 (VIP1 Budding Yeast)
    

    Use

    Tags

    His10-Tag, Twin-Strep-tag, TEV

    Expression

    Insect

    Mutation

    ScVip1-536-1107-R547A

    Promoter

    Available since

    June 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pBB3-ScVip1-536-1107-R551A

    Plasmid

    #238320

    Purpose

    ScVip1-536-1107-R551A protein expression in insect cells

    Depositor

    Michael Hothorn

    Article

    Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

    Insert

    VIP1 (VIP1 Budding Yeast)
    

    Use

    Tags

    His10-Tag, Twin-Strep-tag, TEV

    Expression

    Insect

    Mutation

    ScVip1-536-1107-R551A

    Promoter

    Available since

    June 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBB3-ScVip1-536-1107-E991A

    Plasmid

    #238321

    Purpose

    ScVip1-536-1107-E991A protein expression in insect cells

    Depositor

    Michael Hothorn

    Article

    Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

    Insert

    VIP1 (VIP1 Budding Yeast)
    

    Use

    Tags

    His10-Tag, Twin-Strep-tag, TEV

    Expression

    Insect

    Mutation

    ScVip1-536-1107-E991A

    Promoter

    Available since

    June 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pBB3-ScVip1-536-1107-H651A

    Plasmid

    #238323

    Purpose

    ScVip1-536-1107-H651A protein expression in insect cells

    Depositor

    Michael Hothorn

    Article

    Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

    Insert

    VIP1 (VIP1 Budding Yeast)
    

    Use

    Tags

    His10-Tag, Twin-Strep-tag, TEV

    Expression

    Insect

    Mutation

    ScVip1-536-1107-H651A

    Promoter

    Available since

    June 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pAL007

    Plasmid

    #233866

    Purpose

    MoClo-YTK URA multigene plasmid with MCS; NS3 systems; pTDH3-LexA-NLS-NS3-V1-VP48-2xOaf1-tADH1; lexAO6-pLEU2m-MCS-tENO2

    Depositor

    Jeff Hasty

    Article

    O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

    Insert

    pTDH3-LexA-NLS-NS3-V1-VP48-2xOaf1-tADH1; lexAO6-pLEU2m-MCS-tENO2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pAX1_HS_gpN:SnoopTag

    Plasmid

    #225195

    Purpose

    Used for adding C-terminal SnoopTag to the E. coli HS P2 phage major capsid protein

    Depositor

    Travis Wiles

    Article

    Ortiz de Ora et al Nat Microbiol. 2025 Apr 18. doi: 10.1038

    Insert

    gpN homology arms and SnoopTag
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pAX1_Z11 gpN:SpyTag

    Plasmid

    #225196

    Purpose

    Used for adding C-terminal SpyTag to the Plesiomonas ZOR0011 P2 phage major capsid protein

    Depositor

    Travis Wiles

    Article

    Ortiz de Ora et al Nat Microbiol. 2025 Apr 18. doi: 10.1038

    Insert

    gpN homology arms and SpyTag
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pAX1_HS∆tum

    Plasmid

    #225198

    Purpose

    Used to create a markerless deletion of the E. coli HS tum gene

    Depositor

    Travis Wiles

    Article

    Ortiz de Ora et al Nat Microbiol. 2025 Apr 18. doi: 10.1038

    Insert

    tum homology arms
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pAX1_HS_lexA(Ind-)

    Plasmid

    #225199

    Purpose

    Used to create a non-inducible lexA by introducing a G85D mutation in E. coli HS

    Depositor

    Travis Wiles

    Article

    Ortiz de Ora et al Nat Microbiol. 2025 Apr 18. doi: 10.1038

    Insert

    lexA homology arms
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    lexA G85D

    Promoter

    Available since

    April 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pAX1_HS_gpN:SpyTag

    Plasmid

    #225193

    Purpose

    Used for adding C-terminal SpyTag to the E. coli HS P2 phage major capsid protein

    Depositor

    Travis Wiles

    Article

    Ortiz de Ora et al Nat Microbiol. 2025 Apr 18. doi: 10.1038

    Insert

    gpN homology arms and SpyTag
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pAX1_HS_gpN:SpyTag003

    Plasmid

    #225194

    Purpose

    Used for adding C-terminal SpyTag003 to the E. coli HS P2 phage major capsid protein

    Depositor

    Travis Wiles

    Article

    Ortiz de Ora et al Nat Microbiol. 2025 Apr 18. doi: 10.1038

    Insert

    gpN homology arms and SpyTag003
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. ER-NFAST

    Plasmid

    #233596

    Purpose

    Expression of NFAST on the ER membrane

    Depositor

    Riccardo Filadi

    Article

    Garcia Casas et al Nat Commun. 2024 Nov 12;15(1):9775. doi:

    Insert

    NFAST
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. ER-frNFAST

    Plasmid

    #233599

    Purpose

    Expression of frNFAST on the ER membrane

    Depositor

    Riccardo Filadi

    Article

    Garcia Casas et al Nat Commun. 2024 Nov 12;15(1):9775. doi:

    Insert

    frNFAST
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. PM-frNFAST

    Plasmid

    #233602

    Purpose

    Expression of frNFAST on the PM facing the cytosol

    Depositor

    Riccardo Filadi

    Article

    Garcia Casas et al Nat Commun. 2024 Nov 12;15(1):9775. doi:

    Insert

    frNFAST
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. UBQ10pro-nlsABACUS2-400n-NosT

    Plasmid

    #203728

    Purpose

    Binary vector for the transformation of the nlsABACUS2-400n biosensor for (+)-Abscisic acid into Arabidopsis

    Depositor

    Alexander Jones

    Article

    Rowe et al Nat Plants. 2023 Jun 26. doi: 10.1038/s4

    Insert

    nlsABACUS2-400n
    

    Use

    Synthetic Biology

    Tags

    Myc

    Expression

    Plant

    Mutation

    Promoter

    AtUBQ10pro 1500bp

    Available since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pRS413-TEF1pr-MFA-NFAPoptim

    Plasmid

    #221095

    Purpose

    FAP insert for N-terminally tagging genes of interest (optimized FAP for yeast expression + 2xMYC tag) + MFA1 signal sequence to help target constructs to the ER

    Depositor

    Allyson O’Donnell

    Article

    Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pCARGO-RMCE-Airn

    Plasmid

    #233266

    Purpose

    CARGO plasmid containing ~90kb dox-inducible Airn transgene

    Depositor

    Mauro Calabrese

    Article

    Trotman et al Mol Cell. 2025 Mar 20;85(6):1117-1133.e1

    Insert

    Airn (Airn Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pHAGE-FRB-Fis1 (no mt-mKeima)

    Plasmid

    #223795

    Purpose

    Stable expression of protein in mammalian cell culture

    Depositor

    Sascha Martens

    Article

    Adriaenssens et al bioRxiv [Preprint]. 2024 Aug 28:2024.08.

    Insert

    FRB-Fis1
    

    Use

    Lentiviral

    Tags

    FRB

    Expression

    Mutation

    Promoter

    Available since

    March 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pCAG_WIPI2d-TEV-GST

    Plasmid

    #223799

    Purpose

    Expression of recombinant protein for purification

    Depositor

    James Hurley, Sascha Martens

    Article

    Adriaenssens et al bioRxiv [Preprint]. 2024 Aug 28:2024.08.

    Insert

    WIPI2d (WIPI2 Human)
    

    Use

    Tags

    TEV-GST

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pCAG_GST-TEV-WIPI3

    Plasmid

    #223800

    Purpose

    Expression of recombinant protein for purification

    Depositor

    James Hurley, Sascha Martens

    Article

    Adriaenssens et al bioRxiv [Preprint]. 2024 Aug 28:2024.08.

    Insert

    WIPI3 (WDR45B Human)
    

    Use

    Tags

    GST-TEV

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pscAAV.CAG-EGFP-SV40pA-T7-T3-BC(p1-14)

    Plasmid

    #231346

    Purpose

    Self complementary AAV genome with components for tracking self-complementary AAV genomes via AAV-Zombie. Also expresses EGFP from CAG promoter.

    Depositor

    Viviana Gradinaru

    Article

    Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

    Insert

    T7/T3-BC(p1-14)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pscAAV.BC(p1-6)-CAG-EGFP-SV40pA-BC(p1-6)

    Plasmid

    #231349

    Purpose

    Self complementary AAV genome with concatemerization-dependent barcodes, for tracking AAV concatemers via SpECTr. Also expresses EGFP from CAG promoter.

    Depositor

    Viviana Gradinaru

    Article

    Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

    Insert

    BC(p1-10)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pEW66

    Plasmid

    #232794

    Purpose

    COMPASS Fragment 1 (Cps60, Cps50, Cps35, Cps25, Cps15) in PBIG1a

    Depositor

    Cynthia Wolberger

    Article

    Worden et al Elife. 2020 Jan 10;9. pii: 53199. doi: 1

    Insert

    COMPASS Fragment 1 (Cps60, Cps50, Cps35, Cps25, Cps15) (SWD1, BRE2, SWD2, SDC1, SHG1 Budding Yeast)
    

    Use

    Tags

    No tags

    Expression

    Insect

    Mutation

    Promoter

    polyhedrin promoter

    Available since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pCY5h

    Plasmid

    #160295

    Purpose

    Yeast CRISPR plasmid targeting the hphMX cassette

    Depositor

    Amir Aharoni

    Article

    Soreanu et al Curr Genet. 2018 Oct;64(5):1129-1139. do

    Inserts

    sgRNA expression cassette (with guide targeting hph: GACCTGATGCAGCTCTCGGA)
    URA3 selection cassette
    sgRNA expression cassette (without guide)
    Cas9
    

    Use

    CRISPR

    Tags

    8xHis-tag and SV40 NLS

    Expression

    Mutation

    Promoter

    Available since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pCY5n

    Plasmid

    #160296

    Purpose

    Yeast CRISPR plasmid targeting the natMX cassette

    Depositor

    Amir Aharoni

    Article

    Soreanu et al Curr Genet. 2018 Oct;64(5):1129-1139. do

    Inserts

    sgRNA expression cassette (with guide targeting nat: GTACCGCACCAGTGTCCCGG)
    URA3 selection cassette
    sgRNA expression cassette (without guide)
    Cas9
    

    Use

    CRISPR

    Tags

    8xHis-tag and SV40 NLS

    Expression

    Mutation

    Promoter

    Available since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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43. pRS415-TEF1pr-MFA-NFAPoptim

    Plasmid

    #221090

    Purpose

    FAP insert for N-terminally tagging genes of interest (optimized FAP for yeast expression + 2xMYC tag) + MFA1 signal sequence to help target constructs to the ER

    Depositor

    Allyson O’Donnell

    Article

    Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pCY5k

    Plasmid

    #160294

    Purpose

    Yeast CRISPR plasmid targeting the kanMX cassette

    Depositor

    Amir Aharoni

    Article

    Soreanu et al Curr Genet. 2018 Oct;64(5):1129-1139. do

    Inserts

    sgRNA expression cassette (with guide targeting kan: TGTTTTGCCGGGGATCGCAG)
    URA3 selection cassette
    sgRNA expression cassette (without guide)
    Cas9
    

    Use

    CRISPR

    Tags

    8xHis-tag and SV40 NLS

    Expression

    Mutation

    Promoter

    Available since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pLentiCRISPR-v1-sgMTHFD1

    Plasmid

    #217436

    Purpose

    Lentiviral vector expressing Cas9 and an sgRNA targeting human MTHFD1

    Depositor

    Jason Cantor

    Article

    Flickinger et al Sci Adv. 2024 Oct 4;10(40):eadq3591. doi

    Insert

    sgRNA targeting MTHFD1 (MTHFD1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Jan. 31, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pLJC2-TK2-3xFLAG

    Plasmid

    #217427

    Purpose

    Lentiviral overexpression of human TK2

    Depositor

    Jason Cantor

    Article

    Flickinger et al Sci Adv. 2024 Oct 4;10(40):eadq3591. doi

    Insert

    TK2 (TK2 Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Insert sequence is a codon-optimized gBLOCK (IDT)

    Promoter

    CMV

    Available since

    Jan. 31, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pLentiCRISPR-v1-sgTYMS

    Plasmid

    #217430

    Purpose

    Lentiviral vector expressing Cas9 and an sgRNA targeting human TYMS

    Depositor

    Jason Cantor

    Article

    Flickinger et al Sci Adv. 2024 Oct 4;10(40):eadq3591. doi

    Insert

    sgRNA targeting TYMS (TYMS Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Jan. 31, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pLentiCRISPR-v1-sgTK2_4

    Plasmid

    #217431

    Purpose

    Lentiviral vector expressing Cas9 and an sgRNA targeting human TK2

    Depositor

    Jason Cantor

    Article

    Flickinger et al Sci Adv. 2024 Oct 4;10(40):eadq3591. doi

    Insert

    sgRNA 4 targeting TK2 (TK2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Jan. 31, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pLentiCRISPR-v1-sgTK2_5

    Plasmid

    #217432

    Purpose

    Lentiviral vector expressing Cas9 and an sgRNA targeting human TK2

    Depositor

    Jason Cantor

    Article

    Flickinger et al Sci Adv. 2024 Oct 4;10(40):eadq3591. doi

    Insert

    sgRNA 5 targeting TK2 (TK2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Jan. 31, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pLentiCRISPR-v1-sgSHMT2

    Plasmid

    #217435

    Purpose

    Lentiviral vector expressing Cas9 and an sgRNA targeting human SHMT2

    Depositor

    Jason Cantor

    Article

    Flickinger et al Sci Adv. 2024 Oct 4;10(40):eadq3591. doi

    Insert

    sgRNA targeting SHMT2 (SHMT2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Jan. 31, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge