We narrowed to 50,873 results for: cas

1. pCAG-All-in-one-hCascade

   Plasmid

   #134919

   Purpose

   Components for genome editing in mammalian cells with pPB-CAG-hCas3 and pBS-U6-crRNA-targeted. This plasmid coexpresses PuroR protein.

   Depositor

   Tomoji Mashimo

   Article

   Morisaka et al Nat Commun. 2019 Dec 6;10(1):5302. doi:

   Insert

   Cascade factors (Cse1(Cas8), Cse2(Cas11), Cas5, Cas6, Cas7) with bpNLS
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Feb. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-flex-taCasp3-TEVp

   Plasmid

   #45580

   Depositor

   Jim Wells, Nirao Shah

   Article

   Yang et al Cell. 2013 May 9;153(4):896-909. doi: 10

   Has Service

   AAV1 and AAV5

   Inserts

   Caspase 3 (CASP3 Synthetic, Human)
   Tobacco Etch Virus protease
   

   Use

   AAV; Adeno associated viral vector

   Tags

   Expression

   Mutation

   Linker replaced with a TEV protease cleavage site

   Promoter

   EF1a

   Available since

   July 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMSCV-F-del Casp9.IRES.GFP

   Plasmid

   #15567

   Depositor

   David Spencer

   Article

   Straathof et al Blood. 2005 Jun 1. 105(11):4247-54.

   Insert

   FKBP12(V36)-p30Caspase9 (FKBP1A Human)
   

   Use

   Retroviral

   Tags

   FKBP12 and HA epitope

   Expression

   Mammalian

   Mutation

   FKBP12 F36 to V Truncated Caspase9 (135-416; lac…

   Promoter

   Available since

   Aug. 22, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGEMS-II-dCas13-APO1-YTH

   Plasmid

   #204767

   Purpose

   GEMS system using dCas13-tethered APO1-YTH (pCMV-EGFP-PEST-m6A sensor-DHFR; EF1a-dCas13-NLS-APO1-YTH; hPGK-DsRed)

   Depositor

   Kate Meyer

   Article

   Marayati et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

   Insert

   dCas13-NLS-APO1-YTH-HA
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Ef1alpha

   Available since

   Jan. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. P3-dCas9-Tet1-GFP-Puro

   Plasmid

   #190728

   Purpose

   Expression of human spdCas9-Tet1 fusion for targeted DNA methylation in mammalian cell. EGFP-puromycin double marker under an independent CMV promoter. Cloning backbone for spgRNA (BbsI)

   Depositor

   Maja Jagodic

   Article

   Pahlevan Kakhki et al Nat Commun. 2024 Jul 30;15(1):6419. doi:

   Insert

   dCas9-tet1
   

   Use

   CRISPR

   Tags

   3XFLAG and SV40 NLS

   Expression

   Mammalian

   Mutation

   Changed Pro434 codon from CCG to CCC and Gly468 f…

   Promoter

   pCAG

   Available since

   July 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET-Cas9-NLS-6xHis

   Plasmid

   #62933

   Purpose

   Expression of Cas9-NLS-6xHis in bacterial cells

   Depositor

   David Liu

   Article

   Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

   Insert

   Cas9-NLS-6xHis
   

   Use

   Tags

   6xHis tag and NLS

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1 iCasper T2A HO1

   Plasmid

   #64278

   Purpose

   Expresses infrared fluorescent executioner caspase reporter (iCasper) plus human heme oxygenase 1

   Depositor

   Xiaokun Shu

   Article

   To et al Proc Natl Acad Sci U S A. 2015 Mar 17;11

   Insert

   Infrared fluorescent executioner caspase reporter (iCasper) T2A heme oxygenase-1: (HMOX1 Synthetic)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV-dCas13-inactive M3M14nes

   Plasmid

   #157855

   Purpose

   Targeted m6A RNA methylation in mammalian cells, methyltransferase-inactive mutant

   Depositor

   David Liu

   Article

   Wilson et al Nat Biotechnol. 2020 Jun 29. pii: 10.103

   Insert

   dCas13-inactive M3M14nes (METTL3, METTL14 Cas13b is from Prevotella sp. P5-125; METTL3 and METTL14 are from H. sapiens)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   PspCas13b Δ984-1090 H133A; METTL3 D395A

   Promoter

   Available since

   Aug. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMSCV-dCasRx-ALKBH5-PURO

   Plasmid

   #175582

   Purpose

   Expresses dCasRx-ALKBH5 in mammalian cell

   Depositor

   Qi Xie

   Article

   Xia et al Nucleic Acids Res. 2021 Jul 21;49(13):73

   Insert

   dCasRx-ALKBH5
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG-HA

   Expression

   Mammalian

   Mutation

   RfxCas13d R239A/H244A/R858A/H863A

   Promoter

   pMSCV

   Available since

   Nov. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMSCV-dCasRx-METTL3-PURO

    Plasmid

    #174218

    Purpose

    Expresses dCasRx-METTL3 in mammalian cell

    Depositor

    Qi Xie

    Article

    Xia et al Nucleic Acids Res. 2021 Jul 21;49(13):73

    Insert

    dCasRx-METTL3
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pMSCV

    Available since

    Nov. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. Dual-CRISPR-Cas9-U6-H1 (pBP48)

    Plasmid

    #218932

    Purpose

    The backbone dual-CRISPR plasmid containing Cas9 that is used for making dual-CRISPR plasmids.

    Depositor

    Baoxu Pang

    Article

    Li et al Nat Biomed Eng. 2024 May 22. doi: 10.103

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    2A-Puro and 3XHA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV-SpCas9-P2A-puro

    Plasmid

    #214358

    Purpose

    Expresses SpCas9 and puromycin resistance genes

    Depositor

    Yongming Wang

    Article

    Qi et al Nat Chem Biol. 2023 Dec 5. doi: 10.1038/

    Insert

    SpCas9 and puromycin resistance genes
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSpCas9(BB)-2A-Puro G3BP1-sgRNA

    Plasmid

    #196197

    Purpose

    Cas9 + gRNA expression to edit G3BP1 locus

    Depositor

    Jürgen Knoblich

    Article

    Sidhaye et al Elife. 2023 Mar 29;12:e85135. doi: 10.75

    Insert

    G3BP1 (G3BP1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET-28b-Cas9-His

    Plasmid

    #47327

    Purpose

    For in vitro expression and purification of Cas9 protein

    Depositor

    Alex Schier

    Article

    Gagnon et al PLoS One. 2014 May 29;9(5):e98186. doi:

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. Lenti-dCas9-KRAB-blast

    Plasmid

    #89567

    Purpose

    Plasmid expression dCas9 protein in fusion with KRAB domain

    Depositor

    Gary Hon

    Article

    Xie et al Mol Cell. 2017 Apr 20;66(2):285-299.e5.

    Insert

    dCas9
    

    Use

    Lentiviral

    Tags

    Blasticidin resistance gene and KRAB

    Expression

    Mutation

    D10A and H840A

    Promoter

    Available since

    June 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pC0042-PguCas13b crRNA backbone

    Plasmid

    #103853

    Purpose

    For cloning of guide RNAs compatible with PguCas13b. Contains a 3' direct repeat. Clone using BbsI (BpiI). F overhang cacc. R overhang caac

    Depositor

    Feng Zhang

    Article

    Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

    Insert

    U6-PguCas13b DR-BbsI-BbsI-polyT
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET-dCas9-VP64-6xHis

    Plasmid

    #62935

    Purpose

    Expression of dCas9-VP64-6xHis in bacterial cells

    Depositor

    David Liu

    Article

    Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

    Insert

    dCas9-VP64
    

    Use

    Tags

    3xFLAG, 6xHis, NLS, and VP64

    Expression

    Bacterial

    Mutation

    D10A and H840A amino acid changes render Cas9 nuc…

    Promoter

    T7

    Available since

    May 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. PB-TRE-dCas9-KRAB-MeCP2

    Plasmid

    #122267

    Purpose

    SP-dCas9-KRAB-MeCP2 with doxycycline-inducible expression

    Depositor

    Andrea Califano

    Article

    Tan et al bioRxiv 2021.06.28.449297

    Insert

    dCas9-KRAB-MeCP2
    

    Use

    Tags

    dCas9-KRAB-MeCP2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pC0041-RanCas13b crRNA backbone

    Plasmid

    #103852

    Purpose

    For cloning of guide RNAs compatible with RanCas13b. Contains a 3' direct repeat. Clone using BbsI (BpiI). F overhang cacc. R overhang caac.

    Depositor

    Feng Zhang

    Article

    Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

    Insert

    U6-RanCas13b DR-BbsI-BbsI-polyT
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. HiFi-nCas9-NG_P2A-mCherry

    Plasmid

    #219936

    Purpose

    Mammalian expression of Sp-HiFi-nCas9-NG with P2A-mCherry marker for use with MOBE systems

    Depositor

    Alexis Komor

    Article

    Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

    Insert

    Sp-HiFi-nCas9(D10A)-NG_P2A-mCherry
    

    Use

    CRISPR

    Tags

    SV40 bpNLS and SV40 bpNLS-P2A-mCherry

    Expression

    Mammalian

    Mutation

    HiFi-nCas9(D10A/R691A)

    Promoter

    CMV

    Available since

    June 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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