We narrowed to 97,531 results for: ANG

1. PGL3-U6-RNF2_pegRNA-Csy4RS-nick_sgRNA-EGFP

   Plasmid

   #172669

   Purpose

   For expression of transcript containing pegRNA and nick-sgRNA targeting human RNF2 gene from the U6 promoter with pegRNA flanked by Csy4 recognition site

   Depositor

   Xiaolong Wang

   Article

   Liu et al Cell Res. 2021 Jun 8. pii: 10.1038/s4142

   Insert

   RNF2 pegRNA and RNF2_nick-sgRNA
   

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Aug. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV-SpCas9HF1-P2A-puro

   Plasmid

   #214357

   Purpose

   Expresses SpCas9HF1 and puromycin resistance genes

   Depositor

   Yongming Wang

   Article

   Qi et al Nat Chem Biol. 2023 Dec 5. doi: 10.1038/

   Insert

   SpCas9HF1 and puromycin resistance genes
   

   Expression

   Mammalian

   Mutation

   N497A/R661A/Q695A/Q926A

   Available Since

   Feb. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-ICUE4

   Plasmid

   #181846

   Purpose

   Fourth-generation FRET-based Indicator of cAMP Using Epac. Genetically encoded cAMP indicator. Contains high-sensitivity Epac Q270E mutation.

   Depositor

   Jin Zhang

   Article

   Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

   Insert

   ICUE4 (RAPGEF3 Human)
   

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Mutation

   Glutamine 122 in Epac1 domain mutated to glutamat…

   Promoter

   CMV

   Available Since

   June 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-TNT4-Zfr-P2A-HA-YAP5SA-3xMIR122

   Plasmid

   #223186

   Purpose

   Plasmid containing the Zfr/YAP5SA/3xMIR122 cassette. Translation of YAP5SA is controlled by splicing modulation of the Zfr specifically in cardiomyocytes by risdiplam in vivo.

   Depositor

   Yuxuan Guo

   Article

   Chen et al Nat Cardiovasc Res. 2025 Jun 13. doi: 10

   Inserts

   YAP5SA (Yap1 Mouse)
   mir122 (Mir122 Mouse)
   

   Use

   AAV

   Tags

   HA and engineered P2A

   Mutation

   S61A, S109A, S127A, S164A, S381A

   Promoter

   TNNT2

   Available Since

   June 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-RIα-ICUE4

   Plasmid

   #181848

   Purpose

   ICUE4 cAMP sensor tethered to the PKA RIα subunit.

   Depositor

   Jin Zhang

   Article

   Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

   Insert

   RIα-ICUE4 (RAPGEF3, PRKAR1A Human)
   

   Tags

   ECFP, PKA RIα, and cpVenus(L194)

   Expression

   Mammalian

   Mutation

   Glutamine 122 in Epac1 domain mutated to glutamat…

   Promoter

   CMV

   Available Since

   June 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1(+)-Myr-DnaJB1-PKAcat-mCherry

   Plasmid

   #181853

   Purpose

   mCherry-tagged DnaJB1-PKA catalytic subunit fusion with N-terminal myristoylation site added; for mammalian expression.

   Depositor

   Jin Zhang

   Article

   Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

   Insert

   Myr-DnaJB1-PKAcat-mCherry (PRKACA, DNAJB1 Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMXs-KMS

   Plasmid

   #188038

   Purpose

   Polycistronic human KLF4, MYC and SOX2 were cloned into the pMXs vector

   Depositor

   Young Tang

   Article

   Wang et al Stem Cell Res. 2017 Dec;25:88-97. doi: 1

   Insert

   KLF4, MYC, and SOX2 separated by 2A sequences (KLF4, MYC, SOX2 Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   Oct. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. PCDNA3-FlipGFP(Casp3 cleavage seq) T2A mCherry

   Plasmid

   #124428

   Purpose

   Expresses FlipGFP (caspase-3 cleavage sequence) and T2A mCherry in mammalian cells

   Depositor

   Xiaokun Shu

   Article

   Zhang et al J Am Chem Soc. 2019 Mar 1. doi: 10.1021/

   Insert

   FlipGFP(Casp3 cleavage seq) T2A mCherry
   

   Expression

   Mammalian

   Available Since

   April 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pU6-cj-E sgRNA

   Plasmid

   #169915

   Purpose

   Expression of sgRNA on an optimized gRNA scaffold contains A-U pair flip stabilize the CjCas9/sgRNA complex.

   Depositor

   Zhili Rong

   Article

   Zhang et al Nucleic Acids Res. 2021 Mar 22. pii: 617

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   June 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pST1374-N-NLS-flag-linker-Cas9-D10A

    Plasmid

    #51130

    Purpose

    Cas9 nickase optimized for nuclear import.

    Depositor

    Xingxu Huang

    Article

    Shen et al Nat Methods. 2014 Mar 2. doi: 10.1038/nm

    Insert

    Cas9 nickase
    

    Use

    CRISPR

    Tags

    Flag and NLS

    Expression

    Mammalian

    Mutation

    D10A (nickase mutant)

    Promoter

    CMV

    Available Since

    Feb. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pIHEU-FlipGFP(Casp3 cleavage seq) T2A mCherry

    Plasmid

    #124434

    Purpose

    Expresses FlipGFP (caspase-3 cleavage sequence) and T2A mCherry in Drosophila

    Depositor

    Xiaokun Shu

    Article

    Zhang et al J Am Chem Soc. 2019 Mar 1. doi: 10.1021/

    Insert

    FlipGFP(Casp3 cleavage seq) T2A mCherry
    

    Expression

    Insect

    Available Since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-CAG-C-intein-C-spC9-H840A-N863A-2xNLS-hGH

    Plasmid

    #112211

    Purpose

    Targeted DNA methylation

    Depositor

    Xingxu Huang

    Article

    Lu et al Cell Death Dis. 2020 Feb 3;11(2):85. doi

    Insert

    C-terminus of dCas9
    

    Use

    AAV

    Mutation

    D10A

    Available Since

    Oct. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. PGKp-GFP-YAP (5SA)

    Plasmid

    #174174

    Purpose

    Lentiviral vector to constitutively express overactive YAP (5 LATS phosporylation sites mutated to Ala) under control of the human PGK promoter.

    Depositor

    Rod Bremner

    Article

    Pearson et al Cancer Cell. 2021 Jul 13. pii: S1535-610

    Insert

    YAP (YAP1 Human)
    

    Use

    Lentiviral

    Tags

    3x FLAG tag

    Expression

    Mammalian

    Mutation

    Five LATS phosphorylation sites (S61, S109, S127,…

    Promoter

    PGK

    Available Since

    Aug. 19, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. EFSp-GFP-YAP (5SA)

    Plasmid

    #174170

    Purpose

    Lentiviral vector to constitutively express overactive YAP (5 LATS phosporylation sites mutated to Ala) under control of the EFS promoter.

    Depositor

    Rod Bremner

    Article

    Pearson et al Cancer Cell. 2021 Jul 13. pii: S1535-610

    Insert

    YAP (YAP1 Human)
    

    Use

    Lentiviral

    Tags

    3x FLAG tag

    Expression

    Mammalian

    Mutation

    Five LATS phosphorylation sites (S61, S109, S127,…

    Promoter

    EFS

    Available Since

    Sept. 28, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. LVXN-Neo-NSD2-E1099K

    Plasmid

    #86011

    Purpose

    express NSD2 E1099K mutant in mammalian cells

    Depositor

    Darrin Stuart

    Article

    Jaffe et al Nat Genet. 2013 Nov;45(11):1386-91. doi:

    Insert

    NSD2 E1099K mutant (NSD2 Human)
    

    Tags

    Flag tag  D Y K D D D D K

    Expression

    Mammalian

    Mutation

    E1099K; K1002R (please see depositor comments bel…

    Promoter

    CMV

    Available Since

    Jan. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

16. pcDNA3.1(+) Laccase2 MCS-mFndc3b Mutant miRNAs

    Plasmid

    #126444

    Purpose

    Expresses a mutated version of mouse Fndc3b circular RNA with the miR-93-3p, miR-258-5p, and miR-412-3p target sites all mutated

    Depositor

    Jeremy Wilusz

    Article

    Garikipati et al Nat Commun. 2019 Sep 20;10(1):4317. doi:

    Insert

    Fndc3b (Fndc3b Mouse)
    

    Expression

    Mammalian

    Mutation

    circFndc3b exons are separated by a miniature int…

    Promoter

    CMV

    Available Since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAdeasy-CMV-AE-EF1α-R2

    Plasmid

    #194928

    Purpose

    expressing two fusion proteins of Ag85B-ESAT6 and Rv2031c-Rv2626c from multi-stage antigens of Mycobacterium tuberculosis

    Depositor

    Limei Wang

    Article

    Wang et al Vaccines (Basel). 2024 Sep 6;12(9):1022.

    Inserts

    AE
    EEF1A1 (EEF1A1 Human)
    R2
    

    Use

    Adenoviral

    Tags

    Ag85B-ESAT6, EF1a, and Rv2031c-Rv2626c

    Mutation

    a 48 bp linker (Gly3ser)4 was added between the t…

    Promoter

    CMV and EF1a

    Available Since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLVX-M-puro

    Plasmid

    #125839

    Purpose

    modifying MCS, replacing EcoRI to BamHI region with BamHI and EcoRI

    Depositor

    Boyi Gan

    Article

    Zhang et al Nat Cell Biol. 2018 Oct;20(10):1181-1192

    Type

    Empty backbone

    Use

    Lentiviral

    Available Since

    June 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1(+)-PKI-GFP

    Plasmid

    #118482

    Purpose

    GFP-tagged Protein Kinase A inhibitor peptide.

    Depositor

    Jin Zhang

    Article

    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

    Insert

    PKI-GFP
    

    Tags

    mEGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDisplay-FRISZ

    Plasmid

    #176887

    Purpose

    Far-red fluorescent zinc indicator FRISZ in pDisplay

    Depositor

    Huiwang Ai

    Article

    Wu et al Sci Adv. 2023 Mar;9(9):eadd2058. doi: 10

    Insert

    FRISZ
    

    Tags

    pDisplay backbone elements for export and membran…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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