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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 25,508 results for: crispr

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Showing: 4481 - 4500 of 25508 results

  1. pLCHKO_TK1_HPRT1_Lb_3

    Plasmid
    #155061
    Purpose
    Lentiviral plasmid for expressing U6 driven multiplexed hybrid guide (hg)RNA targeting TK1 using SpCas9 and two intergenic sites & HPRT1 using LbCas12a (CHyMErA system)
    Depositor
    Benjamin Blencowe, Jason Moffat
    Publication
    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.
    Insert
    (hg)RNA targeting TK1 using SpCas9 and two intergenic sites & HPRT1 using LbCas12a
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6
    Available since
    Aug. 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    155061-plasmid-map-sequence-id-308580

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  2. pLCHKO_TK1_HPRT1_Lb_2

    Plasmid
    #155060
    Purpose
    Lentiviral plasmid for expressing U6 driven multiplexed hybrid guide (hg)RNA targeting TK1 using SpCas9 and an intergenic site & HPRT1 using LbCas12a (CHyMErA system)
    Depositor
    Benjamin Blencowe, Jason Moffat
    Publication
    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.
    Insert
    (hg)RNA targeting TK1 using SpCas9 and two intergenic sites & HPRT1 using LbCas12a
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6
    Available since
    Aug. 12, 2020
    Availability
    Academic Institutions and Nonprofits only
    155060-plasmid-map-sequence-id-308523

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  7. M-SP-sgRNA

    Plasmid
    #48671
    Purpose
    Mammalian U6-driven sgRNA (SPm) targeting GTCCCCTCCACCCCACAGTG
    Depositor
    George Church
    Publication
    Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    sgRNA targeting GTCCCCTCCACCCCACAGTG, compatible with S. pyogenes Cas9, hU6 promoter
    Use
    CRISPR
    Promoter
    hU6
    Available since
    Nov. 22, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    48671-plasmid-map-sequence-id-71841

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  13. iSpyMac

    Plasmid
    #155014
    Purpose
    Expresses the codon-optimized, engineered iSpyMac Cas9 nuclease in mammalian cells
    Depositor
    Joseph Jacobson
    Publication
    Chatterjee et al Nat Commun. 2020 May 18;11(1):2474. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    iSpyMac
    Expression
    Mammalian
    Mutation
    PID grafted onto SpyCas9 N-terminal domain, R221K…
    Promoter
    EF1ɑ
    Available since
    Sept. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    155014-plasmid-map-sequence-id-308820

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  14. pGNW2

    Plasmid
    #122086
    Purpose
    suicide vector for integration into Pseudomonas genome after insertion of homologous regions at the multiple cloning site; successful integration causes green fluorescence in cells
    Depositor
    Pablo Ivan Nikel
    Publication
    Wirth et al Microb Biotechnol. 2019 Mar 12. doi: 10.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Bacterial
    Available since
    April 10, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    122086-plasmid-map-sequence-id-451911

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  15. M-SPn-VP64

    Plasmid
    #48674
    Purpose
    Mammalian SP-VP64 nuclease-null Cas9 activator expression, human optimized
    Depositor
    George Church
    Publication
    Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Cas9-VP64, nuclease-null
    Use
    CRISPR
    Tags
    NLS and VP64
    Mutation
    nuclease-null
    Promoter
    CMV
    Available since
    Dec. 9, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by

  20. pCMV-dCpf1-eBE

    Plasmid
    #107688
    Purpose
    Expresses dLbCpf1-eBE in mammalian cells
    Depositor
    Jia Chen
    Publication
    Li et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dLbCpf1-eBE
    Expression
    Mammalian
    Mutation
    dLbCpf1(D832A/E925A/D1148A)
    Promoter
    CMV
    Available since
    April 25, 2018
    Availability
    Academic Institutions and Nonprofits only
    107688-plasmid-map-sequence-id-207603

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Showing: 4481 - 4500 of 25508 results