We narrowed to 30,517 results for: Ide

1. INCTbiosyn-pUB-HspINT7

   Plasmid

   #127515

   Purpose

   Plasmid encodes H. sapiens codon optimized Integrase 7.

   Depositor

   Martin Bonamino

   Article

   Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:

   Insert

   Integrase 7 coding sequence codon optimized for H. sapiens expression.
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   In 2126 position an G mutated for an T changed th…

   Promoter

   Available since

   May 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. INCTbiosyn-p35S-GFP(rc)2

   Plasmid

   #127521

   Purpose

   Plasmid has a CaMV 35S promoter in a forward sequence orientation and an inverted EGFP coding sequence (reverse complement) flanked by attB and attP integrase 2 attachment sites.

   Depositor

   Martin Bonamino

   Article

   Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:

   Insert

   EGFP reverse complement coding sequence flanked by attB/attP Integrase 2 attachment sites.
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   May 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. INCTbiosyn-pEF-GFP(rc)4

   Plasmid

   #127505

   Purpose

   Plasmid has an EF1 alpha promoter in a forward sequence orientation and an inverted EGFP coding sequence (reverse complement) flanked by attB and attP integrase 4 attachment sites.

   Depositor

   Martin Bonamino

   Article

   Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:

   Insert

   EGFP reverse complement coding sequence flanked by attB/attP Integrase 4 attachment sites.
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3-Flag-NSF/T646A

   Plasmid

   #74937

   Purpose

   Mammalian expression of human NSF mutant T646A (mutation in the D2 domain of the protein)

   Depositor

   Elisa Greggio

   Article

   Belluzzi et al Mol Neurodegener. 2016 Jan 13;11(1):1. d

   Insert

   NSF (NSF Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   T646A (mutation in the D2 domain of the protein)

   Promoter

   CMV

   Available since

   April 26, 2016

   Availability

   Academic Institutions and Nonprofits only

5. pcDNA3-Flag-NSF/T645A

   Plasmid

   #74936

   Purpose

   Mammalian expression of human NSF mutant T645A (mutation in the D2 domain of the protein)

   Depositor

   Elisa Greggio

   Article

   Belluzzi et al Mol Neurodegener. 2016 Jan 13;11(1):1. d

   Insert

   NSF (NSF Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   T645A (mutation in the D2 domain of the protein)

   Promoter

   CMV

   Available since

   April 20, 2016

   Availability

   Academic Institutions and Nonprofits only

6. INCTbiosyn-p35S(rc)2_4_5-GFP

   Plasmid

   #127520

   Purpose

   Plasmid has an inverted CaMV 35S promoter sequence (reverse complement) flanked by attB and attP attachment sites of integrases 2, 4, and 5. EGFP coding sequence is in the forward orientation.

   Depositor

   Martin Bonamino

   Article

   Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:

   Insert

   CaMV 35S reverse complement promoter sequence flanked by attB/attP Integrase 2, 4 and 5 attachment sites.
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   May 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. INCTbiosyn-pEF-GFP(rc)Bxb1

   Plasmid

   #127511

   Purpose

   Plasmid has an EF1 alpha promoter in a forward sequence orientation and an inverted EGFP coding sequence (reverse complement) flanked by attB and attP integrase Bxb1 attachment sites.

   Depositor

   Martin Bonamino

   Article

   Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:

   Insert

   EGFP reverse complement coding sequence flanked by attB/attP Integrase Bxb1 attachment sites.
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. INCTbiosyn-pEF-GFP(rc)phiC31

   Plasmid

   #127510

   Purpose

   Plasmid has an EF1 alpha promoter in a forward sequence orientation and an inverted EGFP coding sequence (reverse complement) flanked by attB and attP integrase phiC31 attachment sites.

   Depositor

   Martin Bonamino

   Article

   Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:

   Insert

   EGFP reverse complement coding sequence flanked by attB/attP Integrase phiC31 attachment sites.
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1 IGF1R-BirA(R118G)-HA

   Plasmid

   #109232

   Purpose

   This construct can be used in the BioID process to identify proteins that interact with the Insulin-like Growth Factor 1 Receptor (IGF1R).

   Depositor

   Victor Dzau

   Article

   Bareja et al J Biol Chem. 2018 Mar 12. pii: RA118.002

   Insert

   IGF1R (IGF1R Human)
   

   Use

   Tags

   BirA(R118G) (highly promiscuous form) and HA

   Expression

   Mammalian

   Mutation

   Promoter

   T7

   Available since

   May 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. PB-mCherry-DMDEx23-eGFP

    Plasmid

    #211366

    Purpose

    Piggybac transposon plasmid for a DMD exon 23 skipping reporter (mCherry-DMDEx23)

    Depositor

    Ulrich Lächelt

    Article

    Lessl et al Bioconjug Chem. 2023 Dec 20;34(12):2263-

    Insert

    mCherry-DMDEx23-eGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    mCherry interrupted by mdx dystrophin exon 23 bet…

    Promoter

    CAG

    Available since

    Jan. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA.3.1.ADRB2-NP

    Plasmid

    #134376

    Purpose

    Nanoluc complementation assay. Expression of adrenoceptor beta 2 fused at C termimus with Natural peptide (NP) of NanoLuc. Addition of signal sequence and Flag epitope at N terminus of ADRB2.

    Depositor

    Julien Hanson

    Article

    Laschet et al J Biol Chem. 2019 Mar 15;294(11):4079-40

    Insert

    ADRB2-NP (ADRB2 Human)
    

    Use

    Tags

    Flag, natural peptide of nanoluciferase, and sign…

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    T7

    Available since

    Aug. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pelB-MBP-BB0238-ss

    Plasmid

    #137046

    Purpose

    E. coli expression clone (T7lac promoter) for mature BB0238 (aa 21-256) from B. burgdorferi with N-terminal PelB signal peptide + His10 + mature maltose binding protein + TEV protease cleavage

    Depositor

    Debra Hansen

    Article

    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10

    Insert

    AAC66635.2 (BB_0238 Borrelia burgdorferi B31)
    

    Use

    Tags

    PelB signal peptide + His10 + maltose binding pro…

    Expression

    Bacterial

    Mutation

    mature BB0238: lacks the BB0238 signal peptide

    Promoter

    T7lac

    Available since

    April 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3-NES-YFP-CCTc

    Plasmid

    #184328

    Purpose

    Mammalian expression of the cytosolic localized YFP-CCTd(peptide of Cav1.2)

    Depositor

    Xiaodong Liu

    Article

    Yang et al Commun Biol. 2022 May 19;5(1):484. doi:

    Insert

    CCTc (CACNA1C Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pelB-MBP-P66-ss

    Plasmid

    #137045

    Purpose

    E. coli expression clone (T7lac promoter) for mature P66 (aa 22-618) from B. burgdorferi with N-terminal PelB signal peptide + His10 + mature maltose binding protein + TEV protease cleavage

    Depositor

    Debra Hansen

    Article

    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10

    Insert

    AAC66949.1 (p66 Borrelia burgdorferi B31)
    

    Use

    Tags

    PelB signal peptide + His10 + maltose binding pro…

    Expression

    Bacterial

    Mutation

    mature P66: lacks the P66 signal peptide

    Promoter

    T7lac

    Available since

    April 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSc1-DD

    Plasmid

    #80439

    Purpose

    Expresses eGFP with ecDHFR along with an U6 promoter driven gRNA which cleaves the plasmid in-cell

    Depositor

    Ervin Welker

    Article

    Talas et al DNA Res. 2017 Jun 30. doi: 10.1093/dnare

    Inserts

    sp Cas9 gRNA
    eGFP
    

    Use

    CRISPR

    Tags

    E. coli dihydrofolate reductase

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6

    Available since

    Aug. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pelB-MBP-P13-ss

    Plasmid

    #137065

    Purpose

    E. coli expression clone (T7lac promoter) for mature P13 (aa 22-179) from B. burgdorferi with N-terminal PelB signal peptide + His10 + mature maltose binding protein + TEV protease cleavage

    Depositor

    Debra Hansen

    Article

    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10

    Insert

    AAC66426.1 (BB_0034 Borrelia burgdorferi B31)
    

    Use

    Tags

    PelB signal peptide + His10 + maltose binding pro…

    Expression

    Bacterial

    Mutation

    mature P13: lacks the P13 signal peptide

    Promoter

    T7lac

    Available since

    April 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSH-Csy4-T2A-SpRFN

    Plasmid

    #85754

    Purpose

    Expresses S. pyogenes FokI-dCas9-NLS with a 25 amino acid linker (GGGGS)5 fusion. Also expresses Csy4 for cleavage of multiplexed gRNA transcripts. Derived from pSQT1601 (Addgene #53369).

    Depositor

    Lawrence Stanton

    Article

    Havlicek et al Mol Ther. 2017 Feb 1;25(2):342-355. doi:

    Insert

    Csy4-T2A-FokI-dCas9
    

    Use

    CRISPR

    Tags

    Csy4 and FokI fusion via 25 amino acid (GGGGS)5 l…

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    CAG

    Available since

    Jan. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMXS-IRES-Blast POLE1 N-ISCmut

    Plasmid

    #160803

    Purpose

    Express N-terminal ISC mutant POLE1

    Depositor

    Richard Possemato

    Article

    Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

    Insert

    POLE1 N-ISCmut (POLE Human)
    

    Use

    Retroviral

    Tags

    3xFLAG

    Expression

    Mutation

    shPOLE_1 and shPOLE2 resistant, C651S, C654S, C66…

    Promoter

    Available since

    Jan. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSH-Csy4-T2A-SaRFN

    Plasmid

    #85755

    Purpose

    Expresses S.aureus FokI-dCas9-NLS with a 36 amino acid GSAT linker fusion. Also expresses Csy4 for cleavage of multiplexed gRNA transcripts. Backbone derived from pSQT1601 (Addgene #53369).

    Depositor

    Lawrence Stanton

    Article

    Havlicek et al Mol Ther. 2017 Feb 1;25(2):342-355. doi:

    Insert

    Csy4-T2A-FokI-SadCas9
    

    Use

    CRISPR

    Tags

    3xHA tag, Csy4, and FokI fusion via 36 amino acid…

    Expression

    Mammalian

    Mutation

    D10A, N580A

    Promoter

    CAG

    Available since

    Jan. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. INCTbiosyn-p35S-GFP(rc)phiC31

    Plasmid

    #127527

    Purpose

    Plasmid has a CaMV 35S promoter in a forward sequence orientation and an inverted EGFP coding sequence (reverse complement) flanked by attB and attP integrase phiC31 attachment sites.

    Depositor

    Martin Bonamino

    Article

    Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:

    Insert

    EGFP reverse complement coding sequence flanked by attB/attP Integrase phiC31 attachment sites.
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    May 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge