We narrowed to 10,234 results for: WUS;

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pAAV-CAG-mNeptune2.5-P2A-GFP

Plasmid

#197156

Purpose

Expresses the protein of mNeptune2.5-P2A-GFP in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

mNeptune2.5-P2A-GFP

Use

AAV

Available Since

March 10, 2023

Availability

Academic Institutions and Nonprofits only

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pSIN-LexAOx4-Pmin-mCherry

Plasmid

#192633

Purpose

Lentiviral vector for inducible mCherry expression.

Depositor

Yingxiao Wang

Article

Huang et al Sci Adv. 2020 Feb 19;6(8):eaay9209. doi:

Insert

mCherry

Use

Lentiviral

Expression

Mammalian

Promoter

Minimal promoter

Available Since

Nov. 7, 2022

Availability

Academic Institutions and Nonprofits only

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p5E-pax3a-5.4k

Plasmid

#164966

Purpose

zebrafish pax3a -5.4k promoter

Depositor

GuangJun Zhang

Article

Silic et al Genetics. 2020 Aug;215(4):1067-1084. doi

Insert

pax3a (pax3a Zebrafish)

Use

Gateway 5 end entry vector

Mutation

promoter -5.4k

Available Since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

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pGEM-sgIGR_dual-MTS-3x-Set I

Plasmid

#162764

Purpose

Separately expressing 3 unique sgIGR_dual-MTS (SetI)

Depositor

Antony K Chen

Article

Mao et al Nucleic Acids Res. 2019 Nov 18;47(20):e1

Insert

sgIGR_dual-MTS_1; sgIGR_dual-MTS_2; sgIGR_dual-MTS_3

Expression

Mammalian

Promoter

U6

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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LZF1723: mI12b-EGFP

Plasmid

#104123

Purpose

synOptopatch construct

Depositor

Adam Cohen

Article

Fan et al Nat Methods. 2018 Oct;15(10):823-831. do

Insert

EGFP

Use

Lentiviral

Expression

Mammalian

Promoter

mI12b

Available Since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

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JC031

Plasmid

#104953

Purpose

Methanol inducing hGH, estrogen inducing G-CSF, and IPTG inducing Interferon

Depositor

Timothy Lu

Article

Cao et al Nat Commun. 2018 Jan 8;9(1):77. doi: 10.

Insert

hGH, Interferon and G-CSF

Available Since

June 4, 2018

Availability

Academic Institutions and Nonprofits only

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JC172

Plasmid

#104948

Purpose

Methanol inducing HSA-hGH fusion protein, and estrogen inducing TEV protease

Depositor

Timothy Lu

Article

Cao et al Nat Commun. 2018 Jan 8;9(1):77. doi: 10.

Insert

HSA-hGH fusion protein and TEV protease

Available Since

June 4, 2018

Availability

Academic Institutions and Nonprofits only

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pFA6a-kanMX6-P3nmt1-mEGFP

Plasmid

#105137

Purpose

Yeast gene targeting

Depositor

Jian-Qiu Wu

Article

Laporte et al Mol Biol Cell. 2012 Aug;23(16):3094-110.

Insert

kanMX6-P3nmt1-mEGFP

Tags

3nmt1-mEGFP

Expression

Bacterial and Yeast

Mutation

A206K

Promoter

3nmt1

Available Since

Feb. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX-KG-mTmod3-1-325_domain

Plasmid

#70756

Purpose

mouse Tropomodulin3, 1-325 domain for bacterial expression

Depositor

Weiping Han

Article

Lim et al Nat Commun. 2015 Jan 9;6:5951. doi: 10.1

Insert

Tropomodulin3 1-325 AA fragment (Tmod3 Mouse)

Tags

GST

Expression

Bacterial

Available Since

Feb. 16, 2016

Availability

Academic Institutions and Nonprofits only

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IRS1-A4

Plasmid

#25697

Depositor

Eric Fearon

Article

Bommer et al J Biol Chem. 2010 Jan 15. 285(3):1928-38

Insert

IRS1 (IRS1 Human)

Use

RNAi and Retroviral

Available Since

June 24, 2010

Availability

Academic Institutions and Nonprofits only
pDSM-bc-Ptdh3-CAD-Ttdh1

Plasmid

#127727

Purpose

Yeast pathway position 3. CAD transcription unit with the TDH3 promoter and TDH1 terminator.

Depositor

Christopher Voigt

Article

Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

Insert

cis-aconitate decarboxylase

Use

Synthetic Biology

Expression

Yeast

Promoter

Ptdh3

Available Since

March 1, 2023

Availability

Academic Institutions and Nonprofits only

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pDM_noro_S6_lacZA

Plasmid

#118817

Purpose

T7 RNAP-driven expression of norovirus sense orientation toehold switches (S6) with a lacZ alpha subunit reporter.

Depositor

Alexander Green

Article

Ma et al Synth Biol (Oxf). 2018;3(1):ysy018. doi:

Insert

Norovirus toehold switch S6

Expression

Bacterial

Available Since

Jan. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pYPQ292-D570A

Plasmid

#129679

Purpose

Gateway entry clone for CRISPR-Cas12b systems

Depositor

Yiping Qi

Article

Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

Insert

AaCas12b

Use

CRISPR; Gateway compatible aacas12b entry clone

Expression

Plant

Mutation

D570A; AaCas12b is rice codon optimized

Available Since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

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pFastBac1-LRRK2 WD40

Plasmid

#139211

Purpose

Human LRRK2 WD40 domain (residues 2142–2527) was expressed in Sf9 insect cells via baculovirus infection and purified to homogeneity.

Depositor

Hao Wu

Article

Zhang et al Proc Natl Acad Sci U S A. 2019 Jan 29;11

Insert

LRRK2 (LRRK2 Human)

Tags

N-His and MBP

Expression

Insect

Available Since

March 11, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Flag-PGC-1alpha2

Plasmid

#45502

Depositor

Jorge Ruas, Bruce Spiegelman

Article

Ruas et al Cell. 2012 Dec 7;151(6):1319-31. doi: 10

Insert

PGC-1alpha Isoform2 (Ppargc1a Mouse)

Tags

6xHis and Flag

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pLN481 (FuGW-G8p-IL12-1Pe)

Plasmid

#105191

Purpose

Module 3 - GAL4 synthetic promoter (with eight binding sites) drives the expression of IL12 transcripts containing miR1-Bs(Pe)

Depositor

Timothy Lu

Article

Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

Insert

G8p-IL12-1Pe

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available Since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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pET28A-p53 (1-73) (P to A)

Plasmid

#62069

Purpose

Expression of human p53 (1-73) (all P to A) in e. coli

Depositor

Gary Daughdrill

Article

Borcherds et al Nat Chem Biol. 2014 Dec;10(12):1000-2. d

Insert

p53 (TP53 Human)

Tags

His

Expression

Bacterial

Mutation

Contains TAD residues 1-73; all Prolines changed …

Promoter

T7

Available Since

Jan. 29, 2015

Availability

Academic Institutions and Nonprofits only
pEYFP-N1-hRIP3-FL V460P

Plasmid

#61379

Purpose

Expresses Human full length RIPK3 with V460P mutation in the mammalian cells

Depositor

Francis Chan

Article

Li et al Cell. 2012 Jul 20;150(2):339-50. doi: 10

Insert

Full length human RIP3 with V460Pmutation (RIPK3 Human)

Tags

EYFP

Expression

Mammalian

Mutation

changed V460 to P

Available Since

Jan. 15, 2015

Availability

Academic Institutions and Nonprofits only
pEYFP-N1-hRIP3-FL V458P

Plasmid

#61377

Purpose

Expresses Human full length RIPK3 with V458P mutation in the mammalian cells

Depositor

Francis Chan

Article

Li et al Cell. 2012 Jul 20;150(2):339-50. doi: 10

Insert

Full length human RIP3 with V458Pmutation (RIPK3 Human)

Tags

EYFP

Expression

Mammalian

Mutation

changed V458 to P

Available Since

Jan. 15, 2015

Availability

Academic Institutions and Nonprofits only
pMyc-CMV1-huTLR4mut-C2141A

Plasmid

#53527

Purpose

expression of mutant TLR4 in mammalian cells

Depositor

Linda Yu

Article

Kuo et al Cell Death Differ. 2015 Jan 30. doi: 10.

Insert

TLR4 C2141A (TLR4 Human)

Tags

Myc

Expression

Mammalian

Mutation

C2141A

Promoter

CMV

Available Since

July 25, 2014

Availability

Academic Institutions and Nonprofits only

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