We narrowed to 8,355 results for: construct

1. pUC19-B5XLC1

   Plasmid

   #103088

   Purpose

   Cas9 coding gene template with optimized sequence for human codon usage

   Depositor

   Duhee Bang

   Article

   Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55

   Insert

   B5XLC1(Cas9 coding gene from Streptococcus pyogenes serotype M49 (strain NZ131))
   

   Use

   CRISPR; Cloning vector

   Tags

   Expression

   Mutation

   human codon-optimized

   Promoter

   Available since

   April 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUC19-C4ZA16

   Plasmid

   #103090

   Purpose

   Cas9 coding gene template with optimized sequence for human codon usage

   Depositor

   Duhee Bang

   Article

   Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55

   Insert

   C4ZA16(Cas9 coding gene from Eubacterium rectale (strain ATCC 33656 / VPI 0990))
   

   Use

   CRISPR; Cloning vector

   Tags

   Expression

   Mutation

   human codon-optimized

   Promoter

   Available since

   April 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. KS6A1_HUMAN_D1

   Plasmid

   #79720

   Purpose

   This plasmid encodes the kinase domain of KS6A1. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

   Depositor

   John Chodera, Markus Seeliger, Nicholas Levinson

   Article

   Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

   Insert

   KS6A1 (RPS6KA1 Human)
   

   Use

   Tags

   His10-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

4. KC1G3_HUMAN_D0

   Plasmid

   #79685

   Purpose

   This plasmid encodes the kinase domain of KC1G3. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

   Depositor

   John Chodera, Markus Seeliger, Nicholas Levinson

   Article

   Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

   Insert

   KC1G3 (CSNK1G3 Human)
   

   Use

   Tags

   His10-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

5. 1203A-CMV-5’DR-EGFP-bGHpolyA

   Plasmid

   #221452

   Purpose

   EGFP reporter construct with single unprocessed direct repeat (35nt) at 5' end

   Depositor

   Omar Akbari

   Insert

   eGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. 1203B-CMV-3’DR-EGFP-bGHpolyA

   Plasmid

   #221453

   Purpose

   EGFP reporter construct with single unprocessed direct repeat (35nt) at 3' end

   Depositor

   Omar Akbari

   Insert

   eGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pIVTR(T7G)-PuroR-F2A-mClover3

   Plasmid

   #172291

   Purpose

   In vitro transcription of PuroR-F2A-mClover3 synthetic construct

   Depositor

   Patrik Verstreken

   Insert

   PuroR-F2A-mClover3
   

   Use

   Other

   Tags

   Expression

   Mutation

   Promoter

   T7 promoter G-initiating

   Available since

   Sept. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pANY4

   Plasmid

   #164897

   Purpose

   Two valuable constructs for either IPTG-induced or temperature-induced expression can be simultaneously obtained in a single cloning process by utilizing plasmid pANY4.

   Depositor

   Yingfeng An

   Insert

   ccdB cassette
   

   Use

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pNP172

   Plasmid

   #106363

   Purpose

   To insert a single copy of a construct at a site on Chr II, corresponding to mKate expressed specifically in the epidermis; can be used for N- or C-terminal fusion.

   Depositor

   Jonathan Ewbank

   Insert

   ttTi5605_SEC_col-12p_mkate2 x _3'tbb-2
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   July 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. C321.Ub-UAG-sfGFP

    Bacterial Strain

    #98564

    Purpose

    Recoded strain C321.ΔA (MutS inactivated) with genomically integrated Ubiquitin-UAG-sfGFP reporter

    Depositor

    George Church

    Article

    Kunjapur et al Proc Natl Acad Sci U S A. 2018 Jan 16;11

    Bacterial Resistance

    Ampicillin

    Available since

    Feb. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

11. MZB209

    Plasmid

    #228492

    Purpose

    BirA encoded pACYC vector for co-expression with Avi-tagged constructs (Bacterial Expression)

    Depositor

    Richard Baker

    Insert

    BirA (birA )
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7 Promoter

    Available since

    Jan. 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGL3-U6-XsgRNA-ccdB-EF1a-Puromycin

    Plasmid

    #115519

    Purpose

    To construct multiple sgRNA expression vector for CRISPR

    Depositor

    Xingxu Huang

    Insert

    ccdB-sgRNA scaffold
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCASCADE

    Plasmid

    #65821

    Purpose

    Control guide RNA plasmid E. coli , low phosphate induction

    Depositor

    Michael Lynch

    Insert

    control guideRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    E . coli ugpB gene promoter, low phosphate induc…

    Available since

    July 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBsd-P2a-Fkbp-3xF-GGSG

    Plasmid

    #235514

    Purpose

    N-term tagging construct: clone into ZraI and AfeI sites

    Depositor

    Brian Hendrich

    Insert

    FKBP
    

    Use

    Mouse Targeting

    Tags

    Bsd, FKBP, 3xFLAG

    Expression

    Mutation

    None

    Promoter

    Available since

    June 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGGSG-FKBP-2HA-P2a-Pac

    Plasmid

    #235521

    Purpose

    C-term tagging construct: clone into PmlI and AfeI sites

    Depositor

    Brian Hendrich

    Insert

    FKBP
    

    Use

    Mouse Targeting

    Tags

    FKBP, 2xHA, Puro

    Expression

    Mutation

    None

    Promoter

    Available since

    May 29, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBsd-T2a-Fkbp-3xF-GGSG

    Plasmid

    #235515

    Purpose

    N-term tagging construct: clone into ZraI and AfeI sites

    Depositor

    Brian Hendrich

    Insert

    FKBP
    

    Use

    Mouse Targeting

    Tags

    Bsd, FKBP, 3xFLAG

    Expression

    Mutation

    None

    Promoter

    Available since

    May 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSox2_Halo_Ty2_BactBsd

    Plasmid

    #235513

    Purpose

    C-term HALO targeting construct

    Depositor

    Brian Hendrich

    Insert

    Sox2 (Sox2 Mouse)
    

    Use

    Mouse Targeting

    Tags

    HALO, 2xTy

    Expression

    Mutation

    None

    Promoter

    beta actin

    Available since

    May 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. SARS_pNIC-CTB10H

    Plasmid

    #153056

    Purpose

    Expression construct for producing Avi tagged Seryl-tRNA synthetase

    Depositor

    Susanne Gräslund

    Insert

    SARS (SARS1 Human)
    

    Use

    Tags

    Avi

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. Hsp68-CreER(TM) (DM#264)

    Plasmid

    #15028

    Depositor

    Douglas Melton

    Insert

    Cre-ER(TM)
    

    Use

    Cre/Lox; For making transgenic constructs

    Tags

    Hsp68 promoter and polyA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 18, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDUAL OCLN (GFP)

    Plasmid

    #86982

    Purpose

    Lentiviral expression construct encoding Occludin and GFP from separate promoters

    Depositor

    Joe Grove

    Insert

    OCLN (OCLN Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV/hPGK

    Available since

    Feb. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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