We narrowed to 1,975 results for: ipp

1. pET28a-ybbR-HIS-sfGFP-DocI

   Plasmid

   #58708

   Purpose

   Characteristic fingerprint molecule to perform single molecule force spectroscopy experiments; covalently immobilized via ybbr tag

   Depositor

   Hermann Gaub

   Article

   Otten et al Nat Methods. 2014 Sep 7. doi: 10.1038/nm

   Insert

   sfGFP-DocI
   

   Use

   Tags

   HIS, HRV 3C, and ybbR

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFIN-NRLL-tdTOM

   Plasmid

   #44355

   Depositor

   Susan Semple-Rowland

   Article

   Semple-Rowland et al Mol Vis. 2010 May 27;16:916-34.

   Insert

   NRLL-tdTOM
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   mouse neural leucine zipper (pos 3127-5587)

   Available since

   May 8, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCR8-Magneto2.0

   Plasmid

   #74333

   Purpose

   For gateway cloning Magneto2.0 into destination vector

   Depositor

   Ali Guler

   Article

   Wheeler et al Nat Neurosci. 2016 Mar 7. doi: 10.1038/n

   Insert

   Magneto2.0
   

   Use

   Gateway vector

   Tags

   Flag

   Expression

   Mutation

   TRPV4: delta 760-871

   Promoter

   none

   Available since

   July 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. HA-AMOT p130 deltaCC

   Plasmid

   #32827

   Depositor

   Kunliang Guan

   Article

   Zhao et al Genes Dev. 2011 Jan 1;25(1):51-63.

   Insert

   AMOT p130 (AMOT Human)
   

   Use

   Tags

   2xHA

   Expression

   Mammalian

   Mutation

   E5D, G858D, deletion of amino acids 410-751

   Promoter

   CMV

   Available since

   Jan. 11, 2012

   Availability

   Academic Institutions and Nonprofits only

5. pMH4

   Plasmid

   #52529

   Purpose

   serves as PCR-template to generate HR donors for C-terminal 2xFLAG-tag

   Depositor

   Klaus Foerstemann

   Article

   Bottcher et al Nucleic Acids Res. 2014 Apr 19.

   Insert

   2x FLAG-tag
   

   Use

   Blunt cloning kit

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 14, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDV05.1_pEF1a-iRFP720_frameshifted

   Plasmid

   #200408

   Purpose

   Expresses a mutant iRFP720 mRNA constitutively in mammalian cells. A frameshift deletion in the ORF (compared to pDV05.0) prevents the correct translation of the fluorescent protein

   Depositor

   James Collins

   Article

   Gayet et al Nat Commun. 2023 Mar 11;14(1):1339. doi:

   Insert

   iRFP720 (frameshift mutant)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCA-G-intron-G

   Plasmid

   #40027

   Depositor

   Liqun Luo

   Article

   Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

   Inserts

   GFP exon 1
   GFP exon 2
   beta-globin intron
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   insertion of a loxP site, nucleotides 1-274, and …

   Promoter

   CAG (chicken beta globin promoter and CMV enhance…

   Available since

   Oct. 12, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSCT-GAL93-PSPC1

   Plasmid

   #46321

   Depositor

   Steven Brown

   Article

   Kowalska et al Mol Cell Biol. 2012 Nov;32(22):4585-94.

   Insert

   PSPC1 (PSPC1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 11, 2013

   Availability

   Academic Institutions and Nonprofits only

9. CMV500 A-SREBP1

   Plasmid

   #33357

   Depositor

   Charles Vinson

   Article

   Rishi et al J Biol Chem. 2004 Mar 19;279(12):11863-7

   Insert

   SREBP1 (Srebf1 Cricetulus griseus (hamster))
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Dominant Negative (see notes)

   Promoter

   CMV

   Available since

   Dec. 14, 2011

   Availability

   Academic Institutions and Nonprofits only

10. pSF4 CMV intron renilla TRICK CTE polyA

    Plasmid

    #84443

    Purpose

    TRICK reporter mRNA

    Depositor

    Jeffrey Chao

    Article

    Halstead et al Science. 2015 Mar 20;347(6228):1367-671.

    Insert

    Renill-TRICK
    

    Use

    Luciferase; Frt

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Tet-CMV

    Available since

    Nov. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBT344_pattB-pCA-GFP-pA-FRT5

    Plasmid

    #52550

    Purpose

    for Phic31 integrase-mediated transgene integration;the bacterial backbone can be subsequently removed by crossing to Flp-expressing mice

    Depositor

    Liqun Luo

    Article

    Tasic et al Proc Natl Acad Sci U S A. 2011 May 10;10

    Inserts

    PhiC31o attB (full length)
    GFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAG-AILR-LplA-ER

    Plasmid

    #42574

    Depositor

    Alice Ting

    Article

    Liu et al ACS Nano. 2012 Dec 21;6(12):11080-7. doi

    Insert

    LplA(AILR) with ER-retention sequence KDEL
    

    Use

    Tags

    FLAG

    Expression

    Mutation

    Compared to wild-type LplA: tryptophan 37 to alan…

    Promoter

    CAG

    Available since

    April 11, 2013

    Availability

    Academic Institutions and Nonprofits only

13. pAAV-Linker-hSyn::mCherry.3xFLAG-WPRE

    Plasmid

    #120391

    Purpose

    pAAV plasmid expressing mCherry.3XFLAG under the hSyn promoter with a Linker containing BstBI, BspQI and SacI sites upstream of the hSyn promoter

    Depositor

    Florian Freudenberg

    Article

    Grunewald et al J Atten Disord. 2019 Jan 9:1087054718822

    Type

    Empty backbone

    Use

    AAV

    Tags

    mCherry.3XFLAG

    Expression

    Mutation

    Promoter

    Available since

    Feb. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. Lenti GFP-AMOT p130 Y242/287A

    Plasmid

    #32829

    Depositor

    Kunliang Guan

    Article

    Zhao et al Genes Dev. 2011 Jan 1;25(1):51-63.

    Insert

    AMOT p130 (AMOT Human)
    

    Use

    Lentiviral

    Tags

    GFP-AMOT

    Expression

    Mammalian

    Mutation

    Y242/287A

    Promoter

    Available since

    Feb. 22, 2012

    Availability

    Academic Institutions and Nonprofits only

15. pCA-T-intron-G

    Plasmid

    #36887

    Depositor

    Liqun Luo

    Article

    Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

    Inserts

    T (i.e., ATG, start codon as a first exon)
    GFP
    beta-globin intron
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    deleted nucleotides before nucleotide 275 and ins…

    Promoter

    CAG (chicken beta actin promoter and CMV enhancer)

    Available since

    July 23, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCA-G-intron-T

    Plasmid

    #36885

    Depositor

    Liqun Luo

    Article

    Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

    Inserts

    GFP
    tdTomato-3Myc
    beta-globin intron
    

    Use

    Tags

    3 Myc tags

    Expression

    Mammalian

    Mutation

    deleted nucleotides after nucleotide 274, inserti…

    Promoter

    CAG (chicken beta actin promoter and CMV enhancer)

    Available since

    Aug. 17, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pIW1

    Plasmid

    #52530

    Purpose

    serves as PCR-template to generate HR donors for C-terminal Twin-Strep tag

    Depositor

    Klaus Foerstemann

    Article

    Bottcher et al Nucleic Acids Res. 2014 Apr 19.

    Insert

    Twin-STREP-tag
    

    Use

    Blunt cloning kit

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 14, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pFA6a-TRP1-PGAL1-GFP

    Plasmid

    #41615

    Depositor

    John Pringle

    Article

    Longtine et al Yeast. 1998 Jul;14(10):953-61.

    Insert

    GAL1 promoter (GAL1 Budding Yeast)
    

    Use

    Yeast genomic targeting

    Tags

    GFP(S65T), TRP1 reverse orientation, and yeast AD…

    Expression

    Mutation

    Promoter

    Available since

    Dec. 11, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pHR'-ROMK1-CITE-GFP

    Plasmid

    #21322

    Depositor

    Jay Nadeau

    Article

    Nadeau et al J Neurophysiol. 2000 Aug . 84(2):1062-75

    Insert

    ROMK1 (Kcnj1 Rat)
    

    Use

    Lentiviral

    Tags

    GFP (bicistronic with CITE/IRES)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 15, 2010

    Availability

    Academic Institutions and Nonprofits only

20. pCA-ATG-intron-tdT3Myc

    Plasmid

    #36883

    Depositor

    Liqun Luo

    Article

    Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

    Insert

    tdTomato-3Myc
    

    Use

    Tags

    3 Myc tags

    Expression

    Mammalian

    Mutation

    beta-globin intron containing a loxP site inserte…

    Promoter

    CAG (chicken beta actin promoter and CMV enhancer)

    Available since

    Aug. 8, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pJP102

    Plasmid

    #176480

    Purpose

    Expresses mScarlet under control of the Capsaspora EF1 promoter and NatR under control of the Capsaspora actin promoter

    Depositor

    Duojia Pan

    Article

    Phillips et al Elife. 2022 Jun 6;11. pii: 77598. doi: 1

    Inserts

    actin > NatR
    EF1 > mScarlet
    

    Use

    Capsaspora owczarzaki expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pJP103

    Plasmid

    #176481

    Purpose

    Expresses mScarlet under control of the Capsaspora EF1 promoter and HygR under control of the Capsaspora actin promoter

    Depositor

    Duojia Pan

    Article

    Phillips et al Elife. 2022 Jun 6;11. pii: 77598. doi: 1

    Inserts

    actin > HygR
    EF1 > mScarlet
    

    Use

    Capsaspora owczarzaki expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pRB20

    Plasmid

    #52559

    Purpose

    serves as PCR-template to generate HR donors for C-terminal YFP-tag

    Depositor

    Klaus Foerstemann

    Article

    Bottcher et al Nucleic Acids Res. 2014 Apr 19.

    Insert

    eYFP and Blasticigin resistance cassette
    

    Use

    Blunt cloning kit

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 23, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. MTK2_024

    Plasmid

    #123716

    Purpose

    Encodes crippled mouse pPGK1 as a Type 2 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    pPGK1c
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Crippled Kozac sequence

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pZF-BCB-GCN

    Plasmid

    #127476

    Purpose

    coding sequence for synthetic zinc finger ZF-BCB-GCN

    Depositor

    Sebastian Maerkl

    Article

    Swank et al Proc Natl Acad Sci U S A. 2019 Mar 26;11

    Insert

    ZF-BCB synthetic transcription factor fused with GCN4 leucine zipper
    

    Use

    Cell-free transcription–translation

    Tags

    GCN4 leucine zipper

    Expression

    Mutation

    Promoter

    T7

    Available since

    July 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCAGGS-Hoxc4

    Plasmid

    #45550

    Depositor

    Jeremy Dasen

    Article

    Lacombe et al PLoS Genet. 2013 Jan;9(1):e1003184. doi:

    Insert

    Hoxc4 (Hoxc4 Mouse)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    CMV / b-actin

    Available since

    Aug. 5, 2013

    Availability

    Academic Institutions and Nonprofits only

27. pET28a-ybbR-HIS-CBM-CohI

    Plasmid

    #58709

    Purpose

    Probing molecule to perform single molecule force spectroscopy experiments

    Depositor

    Hermann Gaub

    Article

    Otten et al Nat Methods. 2014 Sep 7. doi: 10.1038/nm

    Insert

    CBM-CohI
    

    Use

    Tags

    HIS, HRV 3C, and ybbR

    Expression

    Bacterial

    Mutation

    changed Alanine 2 to Cysteine in CBM domain

    Promoter

    T7

    Available since

    Aug. 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pBT241_(pCA-ATG-intron-tTA2)

    Plasmid

    #36875

    Depositor

    Liqun Luo

    Article

    Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

    Insert

    tTA2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    insertion of a beta-globin intron with a loxP sit…

    Promoter

    CAG (chicken beta actin promoter and CMV enhancer)

    Available since

    June 25, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pF(UG) hSyn SYP-SELF

    Plasmid

    #158770

    Purpose

    Expression of synaptophysin 1 variant with self-cleaving c-terminal tail.

    Depositor

    Edwin Chapman

    Article

    Vevea et al eLife. 2020 Jun 9;9. pii: 56469. doi: 10

    Insert

    Synaptophysin 1-NS3/4a (Syt1 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Flexible linkers, ADVVCC/QMSY cleavage site

    Promoter

    Available since

    March 31, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pZF-ADD-GCN

    Plasmid

    #127475

    Purpose

    coding sequence for synthetic zinc finger ZF-ADD-GCN

    Depositor

    Sebastian Maerkl

    Article

    Swank et al Proc Natl Acad Sci U S A. 2019 Mar 26;11

    Insert

    ZF-ADD synthetic transcription factor fused with GCN4 leucine zipper
    

    Use

    Cell-free transcription–translation

    Tags

    GCN4 leucine zipper

    Expression

    Mutation

    Promoter

    T7

    Available since

    July 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge