We narrowed to 5,471 results for: pir

1. pTlpA36-Wasabi

   Plasmid

   #86115

   Purpose

   mWasabi reporter gene gated by TlpA36

   Depositor

   Mikhail Shapiro

   Article

   Piraner et al Nat Chem Biol. 2017 Jan;13(1):75-80. doi

   Inserts

   TlpA36
   mWasabi
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   D341A, P60L, D135V, K187R, K202I, L208Q

   Promoter

   pTlpA and pTlpA, LacI

   Available since

   Feb. 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTlpA-Wasabi

   Plasmid

   #85455

   Purpose

   mWasabi reporter gene gated by wild type TlpA

   Depositor

   Mikhail Shapiro

   Article

   Piraner et al Nat Chem Biol. 2017 Jan;13(1):75-80. doi

   Inserts

   TlpA
   mWasabi
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   D341A

   Promoter

   TlpA and TlpA, LacI

   Available since

   Feb. 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pTcI42-Wasabi

   Plasmid

   #86118

   Purpose

   mWasabi reporter gene gated by temperature-sensitive Lambda repressor TcI42

   Depositor

   Mikhail Shapiro

   Article

   Piraner et al Nat Chem Biol. 2017 Jan;13(1):75-80. doi

   Inserts

   TcI42
   mWasabi
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   A67T, K6N, S33T, Y61H, L119P, F122C

   Promoter

   pR/pL and pR/pL, LacI

   Available since

   Feb. 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pTcI-Wasabi

   Plasmid

   #86101

   Purpose

   mWasabi reporter gene gated by temperature-sensitive Lambda repressor TcI

   Depositor

   Mikhail Shapiro

   Article

   Piraner et al Nat Chem Biol. 2017 Jan;13(1):75-80. doi

   Inserts

   TcI
   mWasabi
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   A67T

   Promoter

   pR/pL and pR/pL, LacI

   Available since

   Feb. 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pTlpA-Wasabi-NF

   Plasmid

   #86100

   Purpose

   Nonfluorescent variant of mWasabi reporter gene gated by wild type TlpA

   Depositor

   Mikhail Shapiro

   Article

   Piraner et al Nat Chem Biol. 2017 Jan;13(1):75-80. doi

   Inserts

   TlpA
   mWasabi
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   D341A and S71T, G73A

   Promoter

   TlpA and TlpA, LacI

   Available since

   May 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pTetR193-Wasabi

   Plasmid

   #86105

   Purpose

   mWasabi reporter gene gated by temperature-sensitive TetR I193N

   Depositor

   Mikhail Shapiro

   Article

   Piraner et al Nat Chem Biol. 2017 Jan;13(1):75-80. doi

   Inserts

   TetRts
   mWasabi
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   I193N

   Promoter

   ptetA and ptetR

   Available since

   Feb. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLacI241-Wasabi

   Plasmid

   #86102

   Purpose

   mWasabi reporter gene gated by temperature-sensitive LacI A241T

   Depositor

   Mikhail Shapiro

   Article

   Piraner et al Nat Chem Biol. 2017 Jan;13(1):75-80. doi

   Inserts

   LacIts
   mWasabi
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   A241T

   Promoter

   T7A1O4/O3 and pLacI

   Available since

   Feb. 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pTlpAreverse-Wasabi

   Plasmid

   #86114

   Purpose

   mWasabi reporter gene driven by TlpA promoter in reverse orientation

   Depositor

   Mikhail Shapiro

   Article

   Piraner et al Nat Chem Biol. 2017 Jan;13(1):75-80. doi

   Inserts

   TlpA
   mWasabi
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   D341A

   Promoter

   TlpA (in reverse orientation)

   Available since

   May 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLacI265-Wasabi

   Plasmid

   #86103

   Purpose

   mWasabi reporter gene gated by temperature-sensitive LacI G265D

   Depositor

   Mikhail Shapiro

   Article

   Piraner et al Nat Chem Biol. 2017 Jan;13(1):75-80. doi

   Inserts

   LacIts
   mWasabi
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   G265D

   Promoter

   T7A1O4/O3 and pLacI

   Available since

   Feb. 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pTetR89-Wasabi

    Plasmid

    #86104

    Purpose

    mWasabi reporter gene gated by temperature-sensitive TetR A89D

    Depositor

    Mikhail Shapiro

    Article

    Piraner et al Nat Chem Biol. 2017 Jan;13(1):75-80. doi

    Inserts

    TetRts
    mWasabi
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    A89D

    Promoter

    ptetA and ptetR

    Available since

    Feb. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pTcI38-Wasabi

    Plasmid

    #86117

    Purpose

    mWasabi reporter gene gated by temperature-sensitive Lambda repressor TcI38

    Depositor

    Mikhail Shapiro

    Article

    Piraner et al Nat Chem Biol. 2017 Jan;13(1):75-80. doi

    Inserts

    TcI38
    mWasabi
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    A67T, M1V, L65S, K68R, F115L, D126G, D188G

    Promoter

    pR/pL and pR/pL, LacI

    Available since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCali2

    Plasmid

    #86119

    Purpose

    Multiplex temperature-gated expression of mWasabi and mCherry reporters

    Depositor

    Mikhail Shapiro

    Article

    Piraner et al Nat Chem Biol. 2017 Jan;13(1):75-80. doi

    Inserts

    TlpA36
    mWasabi
    TcI
    mCherry
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    A67T and D341A, P60L, D135V, K187R, K202I, L208Q

    Promoter

    pR/pL, pR/pL, pLacI, pTlpA, and pTlpA, pLacI

    Available since

    Feb. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pThermeleon

    Plasmid

    #86120

    Purpose

    Bandpass temperature-gated expression of mWasabi and mCherry reporters

    Depositor

    Mikhail Shapiro

    Article

    Piraner et al Nat Chem Biol. 2017 Jan;13(1):75-80. doi

    Inserts

    TlpA
    mWasabi
    TcI38
    mCherry
    cI
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    A67T, M1V, L65S, K68R, F115L, D126G, D188G and D3…

    Promoter

    pR/pL, pR/pL, pLacI, and pTlpA

    Available since

    March 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pVRa37_3390

    Plasmid

    #49697

    Depositor

    Christopher Voigt

    Article

    Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

    Insert

    ECF37_3390
    

    Use

    Synthetic Biology

    Tags

    His6-PreScission

    Expression

    Bacterial

    Mutation

    Codon optimized for E.coli

    Promoter

    pT7-lacO

    Available since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pWHI2-HYG

    Plasmid

    #80589

    Purpose

    CEN plasmid expressing full-length WHI2 from S.cerevisiae S288C background under its native promoter

    Depositor

    John McCusker

    Article

    Vijayraghavan et al Genetics. 2018 Nov 29. pii: genetics.118

    Insert

    WHI2 (WHI2 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    WHI2 native promoter

    Available since

    July 28, 2016

    Availability

    Academic Institutions and Nonprofits only

16. pWHI2-NAT

    Plasmid

    #80588

    Purpose

    CEN plasmid expressing full-length WHI2 from S.cerevisiae S288C background under its native promoter

    Depositor

    John McCusker

    Article

    Vijayraghavan et al Genetics. 2018 Nov 29. pii: genetics.118

    Insert

    WHI2 (WHI2 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Native WHI2 promoter

    Available since

    July 28, 2016

    Availability

    Academic Institutions and Nonprofits only

17. pCSN067

    Plasmid

    #101748

    Purpose

    Single copy yeast plasmid expressing Cpf1 from Lachnospiraceae bacterium ND2006 (LbCpf1), codon optimized for expression in Saccharomyces cerevisiae.

    Depositor

    Rene Verwaal

    Article

    Verwaal et al Yeast. 2017 Sep 8. doi: 10.1002/yea.3278

    Inserts

    Cpf1 from Lachnospiraceae bacterium ND2006 (LbCpf1) codon optimized for expression in S. cerevisiae.
    KanMX marker expression cassette.
    

    Use

    CRISPR

    Tags

    SV40 NLS

    Expression

    Yeast

    Mutation

    Promoter

    Heterologous TEF1 promoter from A. gossypii. and …

    Available since

    Dec. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pMal-E6LLO

    Plasmid

    #51281

    Purpose

    Expresses a fibronectin fusion with listeriolysin targeting EGFR in bacteria

    Depositor

    Dane Wittrup

    Article

    Pirie et al Mol Cancer Ther. 2013 Sep;12(9):1774-82.

    Insert

    E6LLO
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pTHSSd_8

    Plasmid

    #59948

    Purpose

    PT7 GFP reporter

    Depositor

    Christopher Voigt

    Article

    Segall-Shapiro et al Mol Syst Biol. 2014 Jul 30;10(7):742.

    Insert

    sfGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    N/A

    Promoter

    PT7

    Available since

    Oct. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pTHSSd_34

    Plasmid

    #59960

    Purpose

    PTac GFP expression

    Depositor

    Christopher Voigt

    Article

    Segall-Shapiro et al Mol Syst Biol. 2014 Jul 30;10(7):742.

    Insert

    sfGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    N/A

    Promoter

    PTac

    Available since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge