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We narrowed to 10,414 results for: 158

Showing: 6871 - 6900 of 10414 results

  1. pFS_0453_pET30_pCat-tetR-Term-ptetA-FsRT-Cas1(opt)-Cas2(opt)_T7term_ARRAY2_FaqI

    Plasmid
    #117006
    Purpose
    Expresses E. coli codon-optimized FsRT-Cas1-Cas2 under ptetA promoter, encodes FsCRISPR array 2, compatible with SENECA acquisition readout
    Depositor
    Randall Platt
    Article
    Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:
    Insert
    Fusicatenibacter saccharivorans RT-Cas1-Cas2
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Nov. 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    117006-plasmid-map-sequence-id-227036

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  18. pAGK61

    Plasmid
    #228121
    Purpose
    Express node 1467 retron with split retron ncRNA and gRNA to edit (10bp barcode) EMX1 gene in human cells
    Depositor
    Seth Shipman
    Article
    Khan et al Nat Biotechnol. 2024 Sep 17. doi: 10.103
    Insert
    Retron node 1467, split retron ncRNA and gRNA to edit EMX1 gene (10bp barcode) in human cells
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG for RT, U6/H1 for ncRNA/gRNA
    Available since
    Dec. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    228121-plasmid-map-sequence-id-455410

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  19. pY2ShHELIX_sgRNA_entry (CJT30)

    Plasmid
    #181781
    Purpose
    Expresses Y2-ShHELIX containing a nicking Y2 I-AniI fusion to ShTnsB. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).
    Depositor
    Benjamin Kleinstiver
    Article
    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038
    Insert
    Y2-nAniI-ShTnsB, ShTnsC, ShTniQ, ShCas12k
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Y2-nAniI = F13Y, S111Y, K227M, F80K, L232K
    Promoter
    Lac and J23119
    Available since
    Feb. 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    181781-plasmid-map-sequence-id-354678

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  20. FRB-CD28TMD-CTEVp(WT)-CS(M)-tTA

    Plasmid
    #137827
    Purpose
    MESA split-TEV protease chain with FRB Rapamycin ectodomain, CD28 transmembrane domain, C-terminal WT TEV protease, M cleavage sequence, and tTA transcription factor
    Depositor
    Joshua Leonard
    Article
    Dolberg et al Nat Chem Biol. 2021 May;17(5):531-539. d
    Insert
    FRB-CD28TMD-CTEVp(WT)-CS(M)-tTA
    Use
    Tags
    3x FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    137827-plasmid-map-sequence-id-311923

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  21. pETSUMO2_Bacteroidetes-bGSDM-TEV

    Plasmid
    #222101
    Purpose
    For expression of a mutant bacterial gasdermin (bGSDM) encoded by a Bacteroidetes species with a TEV protease cleavage site for controlled activation.
    Depositor
    Philip Kranzusch
    Article
    Johnson et al Nature. 2024 Apr;628(8008):657-663. doi:
    Insert
    Bacteroidetes-bGSDM-TEV ORF
    Use
    Tags
    6xHis-SUMO2
    Expression
    Bacterial
    Mutation
    TEV protease site replaces residues E242–G248
    Promoter
    T7
    Available since
    July 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    222101-plasmid-map-sequence-id-440273

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  22. pcDNA3-nuc-Nb37

    Plasmid
    #205515
    Purpose
    Nuclear-targeted Nb37 nanobody that binds and stabilizes the active form of Gαs G-protein subunit.
    Depositor
    Jin Zhang
    Article
    Kwon et al Nature. 2022 Nov;611(7934):173-179. doi:
    Insert
    nuc-Nb37
    Use
    Tags
    Nuclear localization signal (NLS) and mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    205515-plasmid-map-sequence-id-409490

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  28. PYL1_nTEVp

    Plasmid
    #119213
    Purpose
    Expresses N-terminus of tobacco etch virus protease (TEVp) fused to PYL1-abscisic acid responsive protein domain
    Depositor
    Roman Jerala
    Article
    Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d
    Insert
    split nTEVp in sufion with PYL1
    Use
    Tags
    AU1 tag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    March 1, 2019
    Availability
    Academic Institutions and Nonprofits only
    119213-plasmid-map-sequence-id-232030

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  29. ABI_cTEVp

    Plasmid
    #119214
    Purpose
    Expresses C-terminus of tobacco etch virus protease (TEVp) fused to ABI-abscisic acid responsive protein domain
    Depositor
    Roman Jerala
    Article
    Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d
    Insert
    split cTEVp in fusion with ABI
    Use
    Tags
    Myc tag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 22, 2019
    Availability
    Academic Institutions and Nonprofits only
    119214-plasmid-map-sequence-id-231821

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Showing: 6871 - 6900 of 10414 results